Process for the production of fine chemicals

ABSTRACT

The present invention relates to a process for the production of methionine in an organism such as a microorganism, a non-human animal or plant. The invention furthermore relates to nucleic acid molecules, polypeptides, nucleic acid constructs, vectors, antisense molecules, antibodies, host cells, plant tissue, propagation material, harvested material, plants, microorganisms as well as agricultural compositions and to their use.

RELATED APPLICATIONS

This application is a national stage application (under 35 U.S.C. 371)of PCT/EP2005/003297 filed Mar. 30, 2005, which claims benefit ofEuropean application 04008291.9 filed Apr. 6, 2004.

SUBMISSION ON COMPACT DISC

The contents of the following submission on compact discs areincorporated herein by reference in its entirety: two copies of theSequence Listing (COPY 1 and COPY 2) and a computer readable form copyof the Sequence Listing (CRF COPY), all on compact disc, eachcontaining: file name: Sequence Listing -13195-00013-US, date recorded:Oct. 4, 2006, size: 2.05 MB.

The present invention relates to a process for the production ofmethionine in an organism such as a microorganism, a non-human animal orplant. The invention furthermore relates to nucleic acid molecules,polypeptides, nucleic acid constructs, vectors, antisense molecules,antibodies, host cells, plant tissue, propagation material, harvestedmaterial, plants, microorganisms as well as agricultural compositionsand to their use.

Amino acids are used in many branches of industry, including the food,animal feed, cosmetics, pharmaceutical and chemical industries. Aminoacids such as D,L-methionine, L-lysine or L-threonine are used in theanimal feed industry. The essential amino acids valine, leucine,isoleucine, lysine, threonine, methionine, tyrosine, phenylalanine andtryptophan are particularly important for the nutrition of humans and anumber of livestock species. Glycine, L-methionine and tryptophan areall used in the pharmaceutical industry. Glutamine, valine, leucine,isoleucine, histidine, arginine, proline, serine and alanine are used inthe pharmaceutical and cosmetics industries. Threonine, tryptophan andD,L-methionine are widely used feed additives (Leuchtenberger, W. (1996)Amino acids—technical production and use, pp. 466-502 in Rehm et al.,(Ed.) Biotechnology vol. 6, chapter 14a, VCH Weinheim). Moreover, aminoacids are suitable for the chemical industry as precursors for thesynthesis of synthetic amino acids and proteins, such asN-acetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophanand other substances described in Ullmann's Encyclopedia of IndustrialChemistry, vol. A2, pp. 57-97, VCH Weinheim, 1985.

Over one million tonnes of amino acids are currently produced annually;their market value amounts to over 2.5 billion US dollars. They arecurrently produced by four competing processes: Extraction from proteinhydrolysates, for example L-cystine, L-leucine or L-tyrosine, chemicalsynthesis, for example of D,L-methionine, conversion of chemicalprecursors in an enzyme or cell reactor, for example L-phenylalanine,and fermentative production by growing, on an industrial scale, bacteriawhich have been developed to produce and secrete large amounts of thedesired molecule in question. An organism, which is particularlysuitable for this purpose is Corynebacterium glutamicum, which is usedfor example for the production of L-lysine or L-glutamic acid. Otheramino acids which are produced by fermentation are, for example,L-threonine, L-tryptophan, L-aspartic acid and L-phenylalanine.

The biosynthesis of the natural amino acids in organisms capable ofproducing them, for example bacteria, has been characterized thoroughly;for a review of the bacterial amino acid biosynthesis and itsregulation, see Umbarger, H. E. (1978) Ann. Rev. Biochem. 47: 533-606].

It is known that amino acids are produced by fermentation of strains ofcoryneform bacteria, in particular Corynebacterium glutamicum. Due totheir great importance, the production processes are constantly beingimproved. Process improvements can relate to measures regardingtechnical aspects of the fermentation, such as, for example, stirringand oxygen supply, or the nutrient media composition, such as, forexample, the sugar concentration during fermentation, or to the work-upto give the product, for example by ion exchange chromatography, or tothe intrinsic performance properties of the microorganism itself.Bacteria from other genera such as Escherichia or Bacillus are also usedfor the production of amino acids. A number of mutant strains, whichproduce an assortment of desirable compounds from the group of thesulfur-containing fine chemicals have been developed via strainselection. The performance properties of said microorganisms areimproved with respect to the production of a particular molecule byapplying methods of mutagenesis, selection and mutant selection. Methodsfor the production of methionine have also been developed. In thismanner, strains are obtained which are, for example, resistant toantimetabolites, such as, for example, the methionine analoguesα-methylmethionine, ethionine, norleucine, N-acetylnorleucine,S-trifluoromethylhomocysteine, 2-amino-5-heprenoitic acid,selenomethionine, methionine sulfoximine, methoxine,1-aminocyclo-pentanecarboxylic acid or which are auxotrophic formetabolites with regulatory importance and which producesulfur-containing fine chemicals such as, for example, L-methionine.However, such processes developed for the production of methionine havethe disadvantage that their yields are too low for being economicallyexploitable and that they are therefore not yet competitive with regardto chemical synthesis.

Zeh (Plant Physiol., Vol. 127, 2001: 792-802) describes increasing themethionine content in potato plants by inhibiting threonine synthase bywhat is known as antisense technology. This leads to a reduced threoninesynthase activity without the threonine content in the plant beingreduced. This technology is highly complex; the enzymatic activity mustbe inhibited in a very differentiated manner since otherwiseauxotrophism for the amino acid occurs and the plant will no longergrow.

U.S. Pat. No. 5,589,616 teaches another attempt to produce higheramounts of amino acids in plants by overexpressing a monocot storageprotein in dicots. WO 96/38574, WO 97/07665, WO 97/28247, U.S. Pat. Nos.4,886,878, 5,082,993 and U.S. Pat. No. 5,670,635 are following thisapproach. That means in all the aforementioned intellectual propertyrights different proteins or polypeptides are expressed in plants. Saidproteins or polypeptides should function as amino acid sinks. Othermethods for increasing amino acids such as lysine are disclosed in WO95/15392, WO 96/38574, WO 89/11789 or WO 93/19190. In this casesspecific enzymes in the amino acid biosynthetic pathway such as thediphydrodipicolinic acid synthase are deregulated. This leads to anincrease in the production of lysine in the different plants. Anotherapproach to increase the level of amino acids in plants is disclosed inEP-A-0 271 408. EP-A-0 271 408 teaches the mutagenensis of plant andselection afterwards with inhibitors of certain enzymes of amino acidbiosynthetic pathway.

Methods of recombinant DNA technology have also been used for some yearsto improve Corynebacterium strains producing L-amino acids by amplifyingindividual amino acid biosynthesis genes and investigating the effect onamino acid production.

As described above, the essential amino acids are necessary for humansand many mammals, for example for livestock. L-methionine is importantas methyl group donor for the biosynthesis of, for example, choline,creatine, adrenaline, bases and RNA and DNA, histidine, and for thetransmethylation following the formation of S-adenosylmethionine or as asulfhydryl group donor for the formation of cysteine. Moreover,L-methionine appears to have a positive effect in depression.

Improving the quality of foodstuffs and animal feeds is an importanttask of the food-and-feed industry. This is necessary since, forexample, certain amino acids, which occur in plants are limited withregard to the supply of mammals. Especially advantageous for the qualityof foodstuffs and animal feeds is as balanced as possible an amino acidprofile since a great excess of an amino acid above a specificconcentration in the food has no further positive effect on theutilization of the food since other amino acids suddenly becomelimiting. A further increase in quality is only possible via addition offurther amino acids, which are limiting under these conditions. Thetargeted addition of the limiting amino acid in the form of syntheticproducts must be carried out with extreme caution in order to avoidamino acid imbalance. For example, the addition of an essential aminoacid stimulates protein digestion, which may cause deficiency situationsfor the second or third limiting amino acid, in particular. In feedingexperiments, for example casein feeding experiments, the additionalprovision of methionine, which is limiting in casein, has revealed thefatty degeneration of liver, which could only be alleviated after theadditional provision of tryptophan.

To ensure a high quality of foods and animal feeds, it is thereforenecessary to add a plurality of amino acids in a balanced manner to suitthe organism.

It is an object of the present invention to develop an inexpensiveprocess for the synthesis of L-methionine. L-methionine is together withlysine or threonine depending on the organism one of the two aminoacids, which are most frequently limiting.

It was now found that this object is achieved by providing the processaccording to the invention described herein and the embodimentscharacterized in the claims.

Accordingly, in a first embodiment, the invention relates to a processfor the production of a fine chemical, whereby the fine chemical ismethionine. Accordingly, in the present invention, the term “the finechemical” as used herein relates to “methione”. Further, the term “thefine chemicals” as used herein also relates to fine chemicals comprisingmethionine.

In one embodiment, the term “the fine chemical” means L-methionine.Throughout the specification the term “the fine chemical” meansL-methionine, its salts, ester or amids in free form or bound toproteins. In a preferred embodiment, the term “the fine chemical” meansL-methionine in free form or its salts or bound to proteins.

Accordingly, the invention relates to a process for the production ofmethionine, which comprises the following steps:

-   a) reduction or deletion of the biological activity represented by    the protein as depicted in SEQ ID NO: 2, SEQ ID NO: 8, SEQ ID NO:    20, SEQ ID NO: 64, SEQ ID NO: 65, SEQ ID NO: 152, SEQ ID NO: 229 or    SEQ ID NO: 283 in a non-human organism, and-   b) growing the organism under conditions which permit the production    of methionine in said organism.

Advantageously the process for the production of the fine chemical leadsto an enhanced production of said compound. The terms “enhanced” or“increase” mean at least a 10%, 20%, 30%, 40% or 50%, preferably atleast 60%, 70%, 80%, 90% or 100%, more preferably 150%, 200%, 300%, 400%or 500% higher production of the fine chemical in comparison to thereference as defined below that means in comparison to the organismwithout the aforementioned modification of the biological activity ofprotein of the invention.

Preferably, this process further includes the step of recovering thefine chemical, which is synthesized by the organism from the organismand/or from the culture medium used for the growth or maintenance of theorganism.

The term “recovering” means the isolation of the fine chemical indifferent purities, that means on the one hand harvesting of thebiological material, which contains the fine chemical without furtherpurification and on the other hand purities of the fine chemical between5% and 100% purity, preferred purities are in the range of 10% and 95%,more preferred between 20%, 30%, 40% or 50% and 60%, 70%, 80% or 90%.

Surprisingly, the transgenic reduction or deletion of the expression ofthe protein of the invention in Arabidopsis thaliana conferred anincrease in the fine chemical content of the transformed plants.

In accordance with the invention, the term “organism” as understoodherein relates always to a non-human organism, in particular to ananimal or plant organism, the whole organism or cell(s) thereof, or to amicroorganism. Further, the term “animal” as understood herein relatesalways to a non-human animal.

In accordance with the invention, a protein or polypeptide has the“activity or preferably biological activity of the protein as depictedin SEQ ID NO: 2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO:152, SEQ ID NO: 229 or SEQ ID NO: 283” if in the event its de novoactivity or biological activity is reduced or deleted leads to anincreased of the fine chemical. That means the reduction or deletion ofits biological activity for example its enzymatic activity is somehowrelated to the production of the fine chemical. Throughout thespecification the reduction or deletion of the biological activity ofsuch a aforementioned protein or polypeptide or a nucleic acid moleculeor sequence encoding such protein or polypeptide means a reduction ofits biological activity for example its enzymatic activity of at least10% preferably 20%, 30%, 40% or 50%, particularly preferably 60% 70% or80%, most particularly preferably 90%, 91%, 92%, 93%, 94%, 95%, 96%,97%, 98% or 99% in comparison to the biological activity of the proteinas depicted in SEQ ID NO: 2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO: 65,SEQ ID NO: 152, SEQ ID NO: 229 or SEQ ID NO: 283. Throughout thespecification a deletion of the biological activity of the protein asdepicted in SEQ ID NO: 2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO: 65,SEQ ID NO: 152, SEQ ID NO: 229 or SEQ ID NO: 283 means a total loss ofthe activity. The reduction or deletion of the biological activity leadsto an increase of the fine chemical of at least 10%, 20%, 30%, 40%, 50%,100%, 150% or 200%, preferably of at least 250% or 300%, particularlypreferably of at least 350% or 400%, most particularly preferably of atleast 500% or 600% or more.

The terms “reduction”, “decrease” or “deletion” relate to acorresponding change of a property in an organism, a part of an organismsuch as a tissue, seed, root, leave, flower etc. or in a cell. Under“change of a property” it is understood that the activity, expressionlevel or amount of a gene product or the metabolite content is changedin a specific volume or in a specific amount of protein relative to acorresponding volume or amount of protein of a control, reference orwild type. Preferably, the overall activity in the volume is reduced,decreased or deleted in cases if the reduction, decrease or deletion isrelated to the reduction, decrease or deletion of an activity of a geneproduct, independent whether the amount of gene product or the specificactivity of the gene product or both is reduced, decreased or deleted orwhether the amount, stability or translation efficacy of the nucleicacid sequence or gene encoding for the gene product is reduced,decreased or deleted.

The terms “reduction”, “decrease” or “deletion” include the change ofsaid property in only parts of the subject of the present invention, forexample, the modification can be found in compartment of a cell, like anorganelle, or in a part of a plant, like tissue, seed, root, leave,flower etc. but is detectable if the overall subject, i.e. complete cellor plant, is tested. Preferably, the “reduction”, “decrease” or“deletion” is found cellular, thus the term “reduction, decrease ordeletion of an activity” or “reduction, decrease or deletion of ametabolite content” relates to the cellular reduction, decrease ordeletion compared to the wild type cell. In addition the terms“reduction”, “decrease” or “deletion” include the change of saidproperty only during different growth phases of the organism used in theinventive process, for example the reduction, decrease or deletion takesplace only during the seed growth or during blooming. Furthermore theterms include a transitional reduction, decrease or deletion for examplebecause the used RNAi is not stable integrated in the genome of theorganism and has therefore only a transient effect or is for examplecontrolled by an inducible promoter.

Accordingly, the term “reduction”, “decrease” or “deletion” means thatthe specific activity of an enzyme or other protein or regulatory RNA aswell as the amount of a compound or metabolite, e.g. of a polypeptide, anucleic acid molecule or the fine chemical of the invention or anencoding mRNA or DNA, can be reduced, decreased or deleted in a volume.

The terms “wild type”, “control” or “reference” are exchangeable and canbe a cell or a part of organisms such as an organelle or tissue, or anorganism, in particular a microorganism or a plant, which was notmodified or treated according to the herein described process accordingto the invention. Accordingly, the cell or a part of organisms such asan organelle or a tissue, or an organism, in particular a microorganismor a plant used as wild type, control or reference corresponds to thecell, organism or part thereof as much as possible and is in any otherproperty but in the result of the process of the invention as identicalto the subject matter of the invention as possible. Thus, the wild type,control or reference is treated identically or as identical as possible,saying that only conditions or properties might be different which donot influence the quality of the tested property.

Preferably, any comparison is carried out under analogous conditions.The term “analogous conditions” means that all conditions such as, forexample, culture or growing conditions, assay conditions (such as buffercomposition, temperature, substrates, pathogen strain, concentrationsand the like) are kept identical between the experiments to be compared.

The “reference”, “control”, or “wild type” is preferably a subject, e.g.an organelle, a cell, a tissue, an organism, in particular a plant or amicroorganism, which was not modified or treated according to the hereindescribed process of the invention and is in any other property assimilar to the subject matter of the invention as possible. Thereference, control or wild type is in its genome, transcriptome,proteome or metabolome as similar as possible to the subject of thepresent invention. Preferably, the term “reference-” “control-” or “wildtype-”-organelle, -cell, -tissue or -organism, in particular plant ormicroorganism, relates to an organelle, cell, tissue or organism, inparticular plant or microorganism, which is nearly genetically identicalto the organelle, cell, tissue or organism, in particular microorganismor plant, of the present invention or a part thereof preferably 95%,more preferred are 98%, even more preferred are 99.00%, in particular99.10%, 99.30%, 99.50%, 99.70%, 99.90%, 99.99%, 99.999% or more. Mostpreferable the “reference”, “control”, or “wild type” is preferably asubject, e.g. an organelle, a cell, a tissue, an organism, which isgenetically identical to the organism, cell organelle used according tothe process of the invention except that nucleic acid molecules or thegene product encoded by them are changed according to the inventiveprocess.

Preferably, the reference, control or wild type differs form the subjectof the present invention only in the cellular activity of thepolypeptide or RNA of the invention, e.g. as result of a reduction,decrease or deletion in the level of the nucleic acid molecule of thepresent invention or a reduction, decrease or deletion of the specificactivity of the polypeptide or RNA of the invention, e.g. by theexpression level or activity of protein or RNA that means its biologicalactivity and/or its biochemical or genetical causes.

The term “expression” means the transcription of a gene into structuralRNA (rRNA, tRNA, miRNA) or messenger RNA (mRNA) with the subsequenttransaction of the latter into a protein. Experimentally, expression canbe detected by e.g. Northern, qRT PCR, transcriptional run-on assays orWestern blotting and other immuno assays. As consequence of thereduction, decrease or deletion of the expression that means asconsequence of the reduced, decreased or deleted transcription of a genea related phenotypic trait appears such as the enhanced or increasedproduction of the fine chemical.

Accordingly, preferred reference subject is the starting subject of thepresent process of the invention. Preferably, the reference and thesubject matter of the invention are compared after standardization andnormalization, e.g. to the amount of total RNA, DNA, or Protein oractivity or expression of reference genes, like housekeeping genes, suchas ubiquitin.

A series of mechanisms exists via which a modification in thepolypeptide of the invention can directly or indirectly affect theyield, production and/or production efficiency of the amino acid. Forexample, the molecule number or the specific activity of the polypeptideof the invention or the number of expression of the nucleic acidmolecule of the invention may be reduced, decreased or deleted. However,it is also possible to reduce, decrease or delete the expression of thegene which is naturally present in the organisms, for example bymodifying the regulation of the gene, or by reducing or decreasing thestability of the mRNA or of the gene product encoded by the nucleic acidmolecule of the invention.

This also applies analogously to the combined reduction, decrease ordeletion of the expression of the nucleic acid molecule of the presentinvention or its gene product together with the manipulation of furtheractivities such as enzymes of the amino acid biosynthesis pathways.

The reduction, decrease, deletion or modulation according to thisinvention can be constitutive, e.g. due to a stable permanent transgenicexpression or to a stable mutation in the corresponding endogenous geneencoding the nucleic acid molecule of the invention or to a modulationof the expression or of the behaviour of a gene conferring theexpression of the polypeptide of the invention, or transient, e.g. dueto an transient transformation, a transiently active promotor ortemporary addition of a modulator such as an antagonist or inductor,e.g. after transformation with a inducible construct carrying adouble-stranded RNA nucleic acid molecule, an antisense nucleic acidmolecule, a ribozyme of the invention etc. under control of a induciblepromoter and adding the inducer, e.g. tetracycline or as describedherein below.

The reduction, decrease or deletion in activity amounts preferably by atleast 10%, preferably by at least 30% or at least 60%, especiallypreferably by at least 70%, 80%, 85%, 90% or more, very especiallypreferably are at least 95%, more preferably are at least 99% or more incomparison to the control, reference or wild type. Most preferably thereduction, decrease or deletion in activity amounts to 100%.

The specific activity of a polypeptide encoded by a nucleic acidmolecule of the present invention or of the polypeptide of the presentinvention can be tested as described in the examples. In particular, thereduction, decrease or deletion of the expression of a protein inquestion in a cell, e.g. a plant cell or a microorganism and thedetection of an increase of the fine chemical level as well as incomparison to a control is an easy test and can be performed asdescribed in the examples.

The term “reduction”, “decrease” or “deletion” includes, that the reasonfor said “reduction”, “decrease” or “deletion is a chemical compound,which is administered to the organism.

Accordingly, in the following, the term “reducing”, “decreasing” or“deleting” also comprises the term “debasing”, “depleting”, diminishing”or “bringing down”. The decreased or reduced activity manifests itselfin an increase in the fine chemical. In this context, the fine chemicalamount in a cell, preferably in a tissue, more preferred in an organismas a plant or a microorganism or part thereof, is increased by 3% ormore, especially preferably are 10% or more, very especially preferablyare more than 30% and most preferably are 70% or more, such as 100%,300% or 500% or more. The fine chemical can be contained in the organismeither in its free form and/or bound to proteins or polypeptides ormixtures thereof or can be advantageously excreated.

A protein having a biological activity of the proteins used in theinventive process preferably has the structure of the polypeptidedescribed herein, in particular of the polypeptides shown in SEQ ID NO:2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO:12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ IDNO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73,SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO:83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ IDNO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO:111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO:129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO:154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO:172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO:190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO:208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO:256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO:283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO:301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO:319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO:337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO:355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO:373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO:391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO:409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 orSEQ ID NO: 441 or the functional homologues thereof as described herein,or is encoded by the nucleic acid molecule characterized herein or thenucleic acid molecule according to the invention, for example by thenucleic acid molecule shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5,SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15,SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO:27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ IDNO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86,SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO:96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO:114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO:132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155,SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ IDNO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173,SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ IDNO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191,SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ IDNO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209,SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ IDNO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234,SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ IDNO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257,SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ IDNO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284,SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ IDNO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302,SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ IDNO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320,SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ IDNO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338,SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ IDNO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356,SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ IDNO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374,SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ IDNO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392,SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ IDNO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410,SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ IDNO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428,SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or itsherein described functional homologues and has the abovementionedactivity.

For the purposes of the present invention, the terms “L-methionine” ormethionine” also encompass the corresponding salts, such as, forexample, methionine hydrochloride or methionine sulfate. Preferably theterm methionine is intended to encompass the term L-methionine.

Owing to the biological activity of the proteins which are used in theprocess according to the invention and which are encoded by nucleic acidmolecules according to the invention, it is possible to produce aminoacid compositions. Depending on the choice of the organism used for theprocess according to the present invention, for example a microorganismor a plant, compositions or mixtures of various amino acids can beproduced.

The term “expression” refers to the transcription and/or translation ofa codogenic gene segment or gene. As a rule, the resulting product is amRNA or a protein. However, expression products can also includefunctional RNAs such as, for example, antisense, tRNAs, snRNAs, rRNAs,dsRNA, siRNA, miRNAs, ribozymes etc. Expression may be systemic, localor temporal, for example limited to certain cell types, tissues organsor time periods.

In one embodiment, the process of the present invention comprises one ormore of the following steps

-   a) destabilizing a protein enabling the reduced, decreased or    deleted expression of a protein encoded by the nucleic acid molecule    of the invention or of the polypeptide of the invention, e.g. of a    polypeptide having the biological activity of a protein as depicted    in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID    NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20,    SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID    NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,    SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID    NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87,    SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID    NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO:    105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113,    SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ    ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID    NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:    139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154,    SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ    ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID    NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO:    180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188,    SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ    ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID    NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO:    214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229,    SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ    ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID    NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:    260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268,    SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ    ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID    NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO:    303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311,    SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ    ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID    NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO:    337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345,    SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ    ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID    NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO:    371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379,    SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ    ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID    NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:    405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,    SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ    ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID    NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 leading to    the herein-mentioned fine chemical increasing activity; or-   b) destabilizing a mRNA enabling the reduced, decreased or deleted    expression of a protein encoded by the nucleic acid molecule of the    invention, e.g. of a polypeptide having the biological activity of    protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ    ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:    16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ    ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO:    67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ    ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO:    85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ    ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:    103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111,    SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ    ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID    NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO:    137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152,    SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ    ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID    NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO:    178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186,    SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ    ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID    NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO:    212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,    SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ    ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID    NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO:    258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266,    SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ    ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID    NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO:    301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309,    SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ    ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID    NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO:    335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343,    SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ    ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID    NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:    369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,    SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ    ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID    NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO:    403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411,    SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ    ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID    NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID    NO: 441 or of a mRNA encoding the polypeptide of the present    invention leading to the herein-mentioned fine chemical increasing    activity; or-   c) Increasing the biological activity of a protein or RNA e.g.    increasing the biological activity of a repressor enabling the    reduced, decreased or deleted expression of a protein encoded by the    nucleic acid molecule of the invention or of the polypeptide of the    present invention leading to the herein-mentioned fine chemical    increasing activity, e.g. of a polypeptide having the biological    activity of the protein as depicted SEQ ID NO: 2, SEQ ID NO: 4, SEQ    ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,    SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID    NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,    SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID    NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83,    SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID    NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101,    SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ    ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID    NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:    127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,    SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ    ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID    NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:    168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,    SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ    ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID    NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:    202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,    SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ    ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID    NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:    243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256,    SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ    ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID    NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:    291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,    SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ    ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID    NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:    325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333,    SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ    ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID    NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:    359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367,    SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ    ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID    NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:    393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,    SEQ ID NO: 40.3, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ    ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID    NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:    427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435    or SEQ ID NO: 441, or increasing the inhibitory regulation of the    polypeptide of the invention; or-   d) Increasing the biological activity of a protein or RNA e.g.    increasing the biological activity of a repressor enabling the    reduced, decreased or deleted expression of a protein encoded by the    nucleic acid molecule of the present invention or a polypeptide of    the present invention leading to the herein-mentioned fine chemical    increasing activity, e.g. of a polypeptide having the biological    activity of the protein as depicted SEQ ID NO: 2, SEQ ID NO: 4, SEQ    ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,    SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID    NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,    SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID    NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83,    SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID    NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101,    SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ    ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID    NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:    127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,    SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ    ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID    NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:    168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,    SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ    ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID    NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:    202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,    SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ    ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID    NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:    243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256,    SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ    ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID    NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:    291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,    SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ    ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID    NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:    325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333,    SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ    ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID    NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:    359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367,    SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ    ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID    NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:    393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,    SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ    ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID    NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:    427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435    or SEQ ID NO: 441, by adding one or more exogenous repression    factors such as a chemical compound for example an inhibitor to the    organisms or parts thereof; or-   e) reducing, decreasing or deleting the copy number of a gene e.g.    reducing, decreasing or deleting the copy number of a gene encoding    an activator enabling the increased expression of a nucleic acid    molecule encoding a polypeptide encoded by the nucleic acid molecule    of the invention or the polypeptide of the invention having    herein-mentioned fine chemical increasing activity, e.g. of a    polypeptide having the biological activity of the protein as    depicted SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ    ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO:    20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ    ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:    69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ    ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:    87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ    ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO:    105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113,    SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ    ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID    NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:    139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154,    SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ    ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID    NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO:    180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188,    SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ    ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID    NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO:    214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229,    SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ    ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID    NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:    260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268,    SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ    ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID    NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO:    303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311,    SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ    ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID    NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO:    337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345,    SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ    ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID    NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO:    371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379,    SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ    ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID    NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:    405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,    SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ    ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID    NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441; or-   f) reducing, decreasing or deleting the expression of the endogenous    gene encoding the polypeptide of the invention, e.g. a polypeptide    having the biological activity of the protein used in the inventive    process such as the protein as depicted SEQ ID NO: 2, SEQ ID NO: 4,    SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:    14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ    ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:    65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ    ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO:    83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ    ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO:    101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,    SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ    ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID    NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO:    135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143,    SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ    ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID    NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO:    176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,    SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ    ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID    NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO:    210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,    SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ    ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID    NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO:    256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264,    SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ    ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID    NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO:    299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307,    SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ    ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID    NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:    333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,    SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ    ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID    NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:    367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375,    SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ    ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID    NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:    401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409,    SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ    ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID    NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO:    435 or SEQ ID NO: 441, by adding for example an antisense molecule    or RNAi or improving the activity of negative expression elements.    This can be achieved for example through the expression of the    nucleic acids of the invention or parts of it in antisense    orientation or by the expression of hairpin RNAi constructs or the    simultaneous expression of sense and antisense RNA for the nucleic    acids of the invention. Details are described later in the    description or in the examples; or-   g) Increasing the biological activity of a protein or RNA leading to    a dominant negative phenotype of the biological activity of the    nucleic acid molecule of the invention or the protein of the    invention such as a protein as depicted SEQ ID NO: 2, SEQ ID NO: 4,    SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:    14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ    ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:    65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ    ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO:    83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ    ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO:    101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,    SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ    ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID    NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO:    135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143,    SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ    ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID    NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO:    176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,    SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ    ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID    NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO:    210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,    SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ    ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID    NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO:    256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264,    SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ    ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID    NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO:    299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307,    SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ    ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID    NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:    333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,    SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ    ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID    NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:    367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375,    SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ    ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID    NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:    401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409,    SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ    ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID    NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO:    435 or SEQ ID NO: 441. Advantageously this can be achieved for    example through the expression of the nucleic acids encoding a    protein, which has lost its biological activity and which binds to    another protein in a multimeric complex and thereby decreasing or    delting the activity of said complex or which binds for example as a    transcription factor to DNA and thereby decreasing or deleting the    activity of the translated protein; or-   h) expression of an antibody or aptamer, which binds to the nucleic    acid molecule of the invention or the protein of the invention such    as a protein as depicted SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,    SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID    NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26,    SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID    NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75,    SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID    NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93,    SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID    NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO:    111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119,    SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ    ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID    NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO:    152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160,    SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 163, SEQ    ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID    NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:    186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,    SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ    ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID    NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO:    220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235,    SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ    ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID    NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO:    266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283,    SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ    ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID    NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO:    309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317,    SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ    ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID    NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO:    343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351,    SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ    ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID    NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:    377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385,    SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ    ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID    NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO:    411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419,    SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ    ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID    NO: 441 and thereby reducing, decreasing or deleting its biological    activity; and/or-   i) modulating growth conditions of an organism in such a manner,    that the expression or activity of the nucleic acid molecule    encoding the protein of the invention or the protein itself is    reduced, decreased or deleted. This can be achieved by e.g.    modulating light and/or nutrient conditions, which in terms    modulated the expression of the gene or protein of the invention.

Preferably, said mRNA is one kind of the nucleic acid molecule of thepresent invention and/or the protein enabling the reduced or decreasedexpression of a protein encoded by the nucleic acid molecule of thepresent invention or the polypeptide having the herein mentionedbiological activity of the polypeptide of the present invention, e.g.conferring the increase of production of the fine chemical afterdecreasing the expression or activity of the encoded polypeptide orhaving the biological activity of a polypeptide having the biologicalactivity of the protein of the invention.

In general, the amount of mRNA, polynucleotide or nucleic acid moleculein a cell or a compartment of an organism correlates to the amount ofencoded protein and thus with the overall activity of the encodedprotein in said volume. Said correlation is not always linear, theactivity in the volume is dependent on the stability of the molecules,the degradation of the molecules or the presence of activating orinhibiting co-factors. Further, product and educt inhibitions of enzymesare well known.

The activity of the abovementioned proteins and/or polypeptide encodedby the nucleic acid molecule of the present invention can be reduced,decreased or deleted in various ways. For example, the activity in anorganism or in a part thereof, like a cell, is reduced or decreased viareducing or decreasing the gene product number, e.g. by reducing ordecreasing the expression rate, like mutating the natural promoter to alower activity, or by reducing or decreasing the stability of the mRNAexpressed, thus reducing or decreasing the translation rate, and/orreducing or decreasing the stability of the gene product, thusincreasing the proteins decay. Further, the activity or turnover ofenzymes or channels or carriers, transcription factors, and similaractive proteins can be influenced in such a manner that a reduction ofthe reaction rate or a modification (reduction, decrease or deletion) ofthe affinity to the substrate results, is reached. A mutation in thecatalytic centre of an polypeptide of the invention, e.g. as enzyme, canmodulate the turn over rate of the enzyme, e.g. a knock out of anessential amino acid can lead to a reduced or complete knock out of theactivity of the enzyme, or the deletion of regulator binding sites canreduce a negative regulation like a feedback inhibition (or a substrateinhibition, if the substrate level is also increased). The specificactivity of an enzyme of the present invention can be decreased suchthat the turn over rate is decreased or the binding of a co-factor isreduced. Reducing the stability of the encoding mRNA or the protein canalso decrease the activity of a gene product. The reduction of theactivity is also under the scope of the term “reduced, decreased ordeleted activity”. Beside this, advantageously the reduction of theactivity in cis, eg. mutating the promotor including othercis-regulatory elements, or the transcribed or coding parts of the gene,inhibition can be achieved in trans, eg. by transfactors like chimerictranscription factor, ribozymes, antisense RNAs, dsRNAs or dominantnegative proteins versions, which interfere with various stages ofexpression, eg the transcription, the translation or the activity of theprotein or protein complex itself.

In the inventive process as mentioned above preferably the reduction,decrease or deletion of the biological activity represented by proteinof the invention is achieved by reducing, decreasing or delting theexpression of at least one nucleic acid molecule, wherein the nucleicacid molecule is selected from the group consisting of:

-   a) nucleic acid molecule encoding the polypeptide shown in SEQ ID    NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ    ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:    22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ    ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:    71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ    ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO:    89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ    ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID    NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:    115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,    SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ    ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID    NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO:    156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,    SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ    ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID    NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO:    190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198,    SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ    ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID    NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:    231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239,    SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ    ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID    NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:    270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,    SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ    ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID    NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:    313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321,    SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ    ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID    NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:    347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355,    SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ    ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID    NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO:    381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389,    SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ    ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID    NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO:    415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423,    SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ    ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   b) nucleic acid molecule comprising the nucleic acid molecule shown    in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID    NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19,    SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID    NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68,    SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID    NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86,    SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID    NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO:    104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112,    SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ    ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID    NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO:    138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151,    SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ    ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID    NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO:    177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185,    SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ    ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID    NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO:    211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,    SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ    ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID    NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO:    257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265,    SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ    ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID    NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO:    300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308,    SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ    ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID    NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO:    334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342,    SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ    ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID    NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO:    368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376,    SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ    ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID    NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO:    402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410,    SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ    ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID    NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID    NO: 440;-   c) nucleic acid molecule comprising a nucleic acid sequence, which,    as a result of the degeneracy of the genetic code, can be derived    from a polypeptide sequence depicted in SEQ ID NO: 2, SEQ ID NO: 4,    SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:    14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ    ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:    65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ    ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO:    83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ    ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO:    101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,    SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ    ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID    NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO:    135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143,    SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ    ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID    NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO:    176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,    SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ    ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID    NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO:    210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,    SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ    ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID    NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO:    256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264,    SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ    ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID    NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO:    299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307,    SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ    ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID    NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:    333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,    SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ    ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID    NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:    367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375,    SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ    ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID    NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:    401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409,    SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ    ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID    NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO:    435 or SEQ ID NO: 441;-   d) nucleic acid molecule encoding a polypeptide having at least 50%    identity with the amino acid sequence of the polypeptide encoded by    the nucleic acid molecule of (a) to (c) and having the biological    activity represented by protein as depicted in SEQ ID NO: 2, SEQ ID    NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ    ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO:    24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ    ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO:    73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ    ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO:    91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ    ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID    NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO:    117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125,    SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ    ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID    NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO:    158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,    SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ    ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID    NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO:    192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,    SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ    ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID    NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO:    233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241,    SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ    ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID    NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO:    272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289,    SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ    ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID    NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:    315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,    SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ    ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID    NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO:    349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357,    SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ    ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID    NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO:    383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391,    SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ    ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID    NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO:    417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425,    SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ    ID NO: 435 or SEQ ID NO: 441;-   e) nucleic acid molecule which comprises a polynucleotide which is    obtained by amplifying a cDNA library or a genomic library using the    primers depicted in SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ    ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 144, SEQ ID NO:    145, SEQ ID NO: 221, SEQ ID NO: 222, SEQ ID NO: 244, SEQ ID NO: 245,    SEQ ID NO: 436 or SEQ ID NO: 437 and having the biological activity    represented by protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4,    SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:    14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ    ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:    65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ    ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO:    83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ    ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO:    101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,    SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ    ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID    NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO:    135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143,    SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ    ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID    NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO:    176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,    SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ    ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID    NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO:    210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,    SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ    ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID    NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO:    256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264,    SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ    ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID    NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO:    299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307,    SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ    ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID    NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:    333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,    SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ    ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID    NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:    367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375,    SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ    ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID    NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:    401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409,    SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ    ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID    NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO:    435 or SEQ ID NO: 441;-   f) nucleic acid molecule encoding a polypeptide which is isolated    with the aid of monoclonal or polyclonal antibodies against a    polypeptide encoded by one of the nucleic acid molecules of (a) to    (d or e) and having the biological activity represented by the    protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ    ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:    16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ    ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO:    67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ    ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO:    85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ    ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:    103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111,    SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ    ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID    NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO:    137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152,    SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ    ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID    NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO:    178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186,    SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ    ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID    NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO:    212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,    SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ    ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID    NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO:    258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266,    SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ    ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID    NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO:    301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309,    SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ    ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID    NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO:    335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343,    SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ    ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID    NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:    369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,    SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ    ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID    NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO:    403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411,    SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ    ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID    NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID    NO: 441;-   g) nucleic acid molecule encoding a polypeptide comprising the    consensus sequence shown in SEQ ID NO: 33 or SEQ ID NO: 34 and    having the biological activity represented by the protein as    depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8,    SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID    NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,    SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID    NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,    SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID    NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95,    SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID    NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO:    113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,    SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ    ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID    NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO:    154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162,    SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ    ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID    NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO:    188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196,    SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ    ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID    NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:    229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237,    SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ    ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID    NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO:    268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285,    SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ    ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID    NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:    311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319,    SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ    ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID    NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO:    345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353,    SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ    ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID    NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO:    379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387,    SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ    ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID    NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:    413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421,    SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ    ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   h) nucleic acid molecule encoding a polypeptide having the    biological activity represented by the protein as depicted in SEQ ID    NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ    ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:    22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ    ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:    71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ    ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO:    89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ    ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID    NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:    115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,    SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ    ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID    NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO:    156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,    SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ    ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID    NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO:    190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198,    SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ    ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID    NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:    231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239,    SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ    ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID    NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:    270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,    SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ    ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID    NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:    313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321,    SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ    ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID    NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:    347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355,    SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ    ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID    NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO:    381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389,    SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ    ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID    NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO:    415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423,    SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ    ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   i) nucleic acid molecule which is obtainable by screening a suitable    nucleic acid library under stringent hybridisation conditions with a    probe comprising one of the sequences of the nucleic acid molecule    of (a) or (b) or with a fragment thereof having at least 15 nt,    preferably 20 nt, 30 nt, 50 nt, 100 nt, 200 nt or 500 nt of the    nucleic acid molecule characterized in (a) to (c) and encoding a    polypeptide having the biological activity represented by protein of    the invention or which comprises a sequence which is complementary    thereto.

Moreover, the regulation of the abovementioned nucleic acid sequencesmay be modified so that gene expression is decreased. This reduction,decrease or deletion (reduction, decrease, deletion, inactivation ordown-regulation shall be used as synonyms throughout the specification)can be achieved as mentioned above by all methods known to the skilledperson, preferably by double-stranded RNA interference (dsRNAi),introduction of an antisense nucleic acid, a ribozyme, an antisensenucleic acid combined with a ribozyme, a nucleic acid encoding aco-suppressor, a nucleic acid encoding a dominant negative protein, DNA-or protein-binding factors targeting said gene or -RNA or -proteins, RNAdegradation inducing viral nucleic acids and expression systems, systemsfor inducing a homolog recombination of said genes, mutations in saidgenes or a combination of the above.

In general, an activity of a gene product in an organism or partthereof, in particular in a plant cell, a plant, or a plant tissue or apart thereof or in a microorganism can be decreased by decreasing theamount of the specific encoding mRNA or the corresponding protein insaid organism or part thereof. “amount of protein or mRNA” is understoodas meaning the molecule number of polypeptides or mRNA molecules in anorganism, a tissue, a cell or a cell compartment. “Decrease” in theamount of a protein means the quantitative decrease of the moleculenumber of said protein in an organism, a tissue, a cell or a cellcompartment or part thereof—for example by one of the methods describedherein below—in comparison to a wild type, control or reference.

In this context, inactivation means that the enzymatic or biologicalactivity of the polypeptides encoded is no longer detectable in theorganism or in the cell such as, for example, within the plant or plantcell. For the purposes of the invention, downregulation (=reduction)means that the enzymatic or biological activity of the polypeptidesencoded is partly or essentially completely reduced in comparison withthe activity of the untreated organism. This can be achieved bydifferent cell-biological mechanisms. In this context, the activity canbe downregulated in the entire organism or, in the case of multi-celledorganisms, in individual parts of the organism, in the case of plantsfor example in tissues such as the seed, the leaf, the root or otherparts. In this context, the enzymatic activity or biological activity isreduced by at least 10%, advantageously at least 20%, preferably atleast 30%, especially preferably at least 40%, 50% or 60%, veryespecially preferably at least 70%, 80%, 85% or 90% or more, veryespecially preferably are at least 95%, more preferably are at least 99%or more in comparison to the control, reference or wild type. Mostpreferably the reduction, decrease or deletion in activity amounts to100%.

Various strategies for reducing the quantity, the expression, theactivity or the function of proteins encoded by the nucleic acids or thenucleic acid sequences itself according to the invention are encompassedin accordance with the invention. The skilled worker will recognize thata series of different methods are available for influencing the quantityof a protein, the activity or the function in the desired manner.

The term “biological activity” means the biological function of theprotein of the invention. In contrast to the term “biological activity”the term “activity” means the increase in the production of the compoundproduced by the inventive process. The term “biological activity”preferably refers to for example the enzymatic function, transporter orcarrier function, DNA-packaging function, heat shock protein function,recombination protein function or regulatory function of a peptide orprotein in an organism, a tissue, a cell or a cell compartment. Suitablesubstrates are low-molecular-weight compounds and also the proteininteraction partners of a protein. The term “reduction” of thebiological function refers, for example, to the quantitative reductionin binding capacity or binding strength of a protein for at least onesubstrate in an organism, a tissue, a cell or a cell compartment—forexample by one of the methods described herein below—in comparison withthe Wild type of the same genus and species to which this method has notbeen applied, under otherwise identical conditions (such as, forexample, culture conditions, age of the plants and the like). Reductionis also understood as meaning the modification of the substratespecificity as can be expressed for example, by the kcat/Km value. Inthis context, a reduction of the function of at least 10%,advantageously of at least 20%, preferably at least 30%, especiallypreferably of at least 40%, 50% or 60%, very especially preferably of atleast 70%, 80%, 90% or 95%, in comparison with the untreated organism isadvantageous. A particularly advantageous embodiment is the inactivationof the function. Binding partners for the protein can be identified inthe manner with which the skilled worker is familiar, for example by theyeast 2-hybrid system.

A modification, i.e. a decrease, can be caused by endogenous orexogenous factors. For example, a decrease in activity in an organism ora part thereof can be caused by adding a chemical compound such as anantagonist to the media, nutrition, soil of the plants or to the plantsthemselves.

In one embodiment the increase in the fine chemical can be achieved bydecreasing the endogenous level of the polypeptide of the invention.Accordingly, in an embodiment of the present invention, the presentinvention relates to a process, wherein the gene copy number of a geneencoding the polynucleotide or nucleic acid molecule of the invention isdecreased. Further, the endogenous level of the polypeptide of theinvention can for example be decreased by modifying the transcriptionalor translational regulation of the polypeptide.

A further embodiment of the inventive process is a process, whereby thereduction or deletion of the biological activity represented by theprotein used in the inventive process is achieved by a processcomprising a step selected from the group consisting of:

-   (a) introducing of a nucleic acid molecule encoding a ribonucleic    acid sequence, which are able to form double-stranded ribonucleic    acid molecules, whereby the sense strand of said double-stranded    ribonucleic acid molecules has a homology of at least 30% to a    nucleic acid molecule conferring the expression of or encoding a    protein having the biological activity of the protein as depicted in    SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO:    10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ    ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO.    30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ    ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:    79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ    ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO:    97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,    SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ    ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID    NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO:    131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139,    SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ    ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID    NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO:    172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180,    SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ    ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID    NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO:    206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214,    SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ    ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID    NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:    252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,    SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ    ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID    NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO:    295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303,    SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ    ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID    NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO:    329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337,    SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ    ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID    NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO:    363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371,    SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ    ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID    NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO:    397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405,    SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ    ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID    NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:    431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 or comprising    a fragment of at least 17, 18, 19, 20, 21, 22, 23, 24 or 25 base    pairs of a nucleic acid molecule with a homology of at least 50% to    a nucleic acid molecule conferring the expression of a protein    having the biological activity of the protein as depicted in SEQ ID    NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ    ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:    22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ    ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:    71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ    ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO:    89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ    ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID    NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:    115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,    SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ    ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID    NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO:    156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,    SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ    ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID    NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO:    190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198,    SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ    ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID    NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:    231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239,    SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ    ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID    NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:    270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,    SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ    ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID    NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:    313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321,    SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ    ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID    NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:    347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355,    SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ    ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID    NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO:    381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389,    SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ    ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID    NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO:    415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423,    SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ    ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   (b) introducing an antisense nucleic acid molecule, whereby the    antisense nucleic acid molecule has a homology of at least 30% to a    nucleic acid molecule antisense to a nucleic acid molecule encoding    a protein having the biological activity of the protein as depicted    in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID    NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20,    SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID    NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,    SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID    NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87,    SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID    NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO:    105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113,    SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ    ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID    NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:    139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154,    SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ    ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID    NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO:    180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188,    SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ    ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID    NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO:    214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229,    SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ    ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID    NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:    260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268,    SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ    ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID    NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO:    303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311,    SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ    ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID    NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO:    337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345,    SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ    ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID    NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO:    371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379,    SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ    ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID    NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:    405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,    SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ    ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID    NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 or    conferring the expression of a protein having the biological    activity of a protein having the biological activity of the protein    as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:    8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ    ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO:    28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ    ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO:    77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ    ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO:    95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103,    SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ    ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID    NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO:    129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137,    SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ    ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID    NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO:    170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178,    SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ    ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID    NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:    204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,    SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ    ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID    NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO:    250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258,    SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ    ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID    NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO:    293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301,    SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ    ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID    NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO:    327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335,    SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ    ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID    NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO:    361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369,    SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ    ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID    NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:    395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403,    SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ    ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID    NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO:    429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:    441 or introducing an antisense nucleic acid molecule comprising a    fragment of at least 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25    base pairs of a nucleic acid molecule with a homology of at least    50% to an antisense nucleic acid molecule to a nucleic acid molecule    conferring the expression of a protein having the biological    activity of protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ    ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,    SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID    NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,    SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID    NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83,    SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID    NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101,    SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ    ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID    NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:    127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,    SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ    ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID    NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:    168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,    SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ    ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID    NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:    202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,    SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ    ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID    NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:    243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256,    SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ    ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID    NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:    291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,    SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ    ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID    NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:    325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333,    SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ    ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID    NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:    359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367,    SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ    ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID    NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:    393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,    SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ    ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID    NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:    427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435    or SEQ ID NO: 441;-   (c) introducing of a ribozyme which specifically cleaves a nucleic    acid molecule conferring expression of a protein having the    biological activity of protein as depicted in SEQ ID NO: 2, SEQ ID    NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ    ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO:    24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ    ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO:    73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ    ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO:    91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ    ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID    NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO:    117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125,    SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ    ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID    NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO:    158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,    SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ    ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID    NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO:    192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,    SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ    ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID    NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO:    233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241,    SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ    ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID    NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO:    272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289,    SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ    ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID    NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:    315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,    SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ    ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID    NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO:    349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357,    SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ    ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID    NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO:    383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391,    SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ    ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID    NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO:    417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425,    SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ    ID NO: 435 or SEQ ID NO: 441;-   (d) introducing of the antisense nucleic acid molecule characterized    in (b) and the ribozyme characterized in (c);-   (e) introducing of a sense nucleic acid molecule conferring the    expression of a nucleic acid molecule as depicted in SEQ ID NO: 1,    SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO:    11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ    ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO:    31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ    ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO:    80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ    ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO:    98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106,    SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ    ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID    NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO:    132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140,    SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID    NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO:    163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171,    SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ    ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID    NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO:    197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205,    SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ    ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID    NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO:    238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251,    SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ    ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID    NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO:    286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,    SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ    ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID    NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO:    320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328,    SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ    ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID    NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO:    354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362,    SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ    ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID    NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO:    388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396,    SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ    ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID    NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO:    422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430,    SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or a nucleic acid    molecule encoding a polypeptide or part of it (need not to encode a    functional protein) having at least 50% identity with the amino acid    sequence of the polypeptide encoded by the nucleic acid molecule    used in the inventive process and having the biological activity    represented by the protein as depicted in SEQ ID NO: 2, SEQ ID NO:    4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID    NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24,    SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID    NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73,    SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID    NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91,    SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID    NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO:    109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117,    SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ    ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID    NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:    143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,    SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ    ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID    NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO:    184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192,    SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ    ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID    NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO:    218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233,    SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ    ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID    NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO:    264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272,    SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ    ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID    NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO:    307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315,    SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ    ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID    NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:    341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349,    SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ    ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID    NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO:    375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383,    SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ    ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID    NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO:    409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417,    SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ    ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID    NO: 435 or SEQ ID NO: 441 for inducing a co-suppression of the    endogenous protein having a biological activity of the protein as    depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8,    SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID    NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,    SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID    NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,    SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID    NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95,    SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID    NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO:    113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,    SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ    ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID    NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO:    154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162,    SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ    ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID    NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO:    188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196,    SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ    ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID    NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:    229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237,    SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ    ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID    NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO:    268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285,    SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ    ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID    NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:    311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319,    SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ    ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID    NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO:    345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353,    SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ    ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID    NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO:    379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387,    SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ    ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID    NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:    413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421,    SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ    ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   (f) introducing a nucleic acid molecule conferring the expression of    a dominant-negative mutant of a protein having the biological    activity of a protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ    ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,    SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID    NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,    SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID    NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83,    SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID    NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101,    SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ    ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID    NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:    127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,    SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ    ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID    NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:    168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,    SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ    ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID    NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:    202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,    SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ    ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID    NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:    243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256,    SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ    ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID    NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:    291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,    SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ    ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID    NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:    325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333,    SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ    ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID    NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:    359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367,    SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ    ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID    NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:    393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,    SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ    ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID    NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:    427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435    or SEQ ID NO: 441, that means by expressing said sequence leading to    the dominant-negative mutant protein thereby the biological activity    of the protein used in the inventive process is reduced, decreased    or deleted and therefore the production of the fine chemical is    increased;-   (g) introducing a nucleic acid molecule encoding a factor, which    binds to a nucleic acid molecule conferring the expression of a    protein having the biological activity of a protein as depicted in    SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO:    10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ    ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO.    30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ    ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:    79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ    ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO:    97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,    SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ    ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID    NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO:    131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139,    SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ    ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID    NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO:    172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180,    SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ    ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID    NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO:    206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214,    SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ    ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID    NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:    252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,    SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ    ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID    NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO:    295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303,    SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ    ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID    NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO:    329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337,    SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ    ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID    NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO:    363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371,    SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ    ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID    NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO:    397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405,    SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ    ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID    NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:    431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   (h) introducing a viral nucleic acid molecule conferring the decline    of a RNA molecule conferring the expression of a protein having the    biological activity of a protein used in the inventive process    especially a protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ    ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,    SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID    NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,    SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID    NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83,    SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID    NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101,    SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ    ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID    NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:    127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,    SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ    ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID    NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:    168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,    SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ    ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID    NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:    202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,    SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ    ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID    NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:    243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256,    SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ    ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID    NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:    291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,    SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ    ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID    NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:    325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333,    SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ    ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID    NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:    359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367,    SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ    ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID    NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:    393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,    SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ    ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID    NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:    427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435    or SEQ ID NO: 441;-   (i) introducing a nucleic acid construct capable to recombinant with    a endogenous gene conferring the expression of a protein having the    biological activity of a protein used in the inventive process    especially a protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ    ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,    SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID    NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,    SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID    NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83,    SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID    NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101,    SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ    ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID    NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:    127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,    SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ    ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID    NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:    168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,    SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ    ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID    NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:    202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,    SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ    ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID    NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:    243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256,    SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ    ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID    NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:    291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,    SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ    ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID    NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:    325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333,    SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ    ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID    NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:    359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367,    SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ    ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID    NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:    393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,    SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ    ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID    NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:    427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435    or SEQ ID NO: 441;-   (j) introducing a non-silent mutation in an endogenous gene    conferring the expression of a protein having the biological    activity of a protein used in the inventive process especially a    protein as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ    ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:    16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ    ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO:    67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ    ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO:    85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ    ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:    103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111,    SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ    ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID    NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO:    137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152,    SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ    ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID    NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO:    178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186,    SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ    ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID    NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO:    212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,    SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ    ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID    NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO:    258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266,    SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ    ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID    NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO:    301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309,    SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ    ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID    NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO:    335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343,    SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ    ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID    NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:    369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,    SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ    ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID    NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO:    403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411,    SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ    ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID    NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID    NO: 441;-   (k) selecting of a non-silent mutation in a nucleic acid sequence    encoding a protein having the biological activity of a protein used    in the inventive process especially a protein as depicted in SEQ ID    NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ    ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:    22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ    ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:    71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ    ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO:    89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ    ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID    NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:    115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,    SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ    ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID    NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO:    156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,    SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ    ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID    NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO:    190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198,    SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ    ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID    NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:    231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239,    SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ    ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID    NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:    270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,    SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ    ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID    NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:    313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321,    SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ    ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID    NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:    347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355,    SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ    ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID    NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO:    381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389,    SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ    ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID    NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO:    415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423,    SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ    ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 from a randomly    mutagenized population of organisms used in the inventive process;    and/or-   (l) introducing an expression construct conferring the expression of    nucleic acid molecule characterized in any one of (a) to (k).

As the skilled person knows it is possible starting from the nucleicacid sequences mentioned under point (a) to (j) above it is easypossible to isolate the 5′- and/or 3′-regions of said nucleic acidsequences and to use said 5′- and/or 3′-sequences for the reduction,decrease or deletion of the nucleic acid sequences used in the inventiveprocess according to the different process steps (a) to (j) mentionedabove. Preferably less than 1000 bp, 900 bp, 800 bp or 700 bp,particular preferably less than 600 bp, 500 bp, 400 bp, 300 bp, 200 bpor 100 bp of the 5′- and/or 3′-region of the said nucleic acid sequenceare used.

The aforementioned process steps of the reduction or deletion of thebiological activity represented by the protein of the invention lead toan increase of the production of the fine chemical.

A reduction in the activity or the function is preferably achieved by areduced expression of a gene encoding the protein of the inventiveprocess.

Further preferred embodiments of the invention to reduce the biologicalactivity of the protein of the inventive process, said reduction of theactivity or function can be achieved using the following methods:

-   a) introduction of a double-stranded RNA nucleic acid sequence    (dsRNA) as described above or of an expression cassette, or more    than one expression cassette, ensuring the expression of the latter;-   b) introduction of an antisense nucleic acid sequence or of an    expression cassette ensuring the expression of the latter.    Encompassed are those methods in which the antisense nucleic acid    sequence is directed against a gene (i.e. genomic DNA sequences) or    a gene transcript (i.e. RNA sequences) including the 5′ and    3′non-translated regions. Also encompassed are α-anomeric nucleic    acid sequences;-   c) introduction of an antisense nucleic acid sequence in combination    with a ribozyme or of an expression cassette ensuring the expression    of the former;-   d) introduction of sense nucleic acid sequences for inducing    cosuppression or of an expression cassette ensuring the expression    of the former;-   e) introduction of a nucleic acid sequence encoding    dominant-negative protein or of an expression cassette ensuring the    expression of the latter;-   f) introduction of DNA-, RNA- or protein-binding factors against    genes, RNA's or proteins or of an expression cassette ensuring the    expression of the latter;-   g) introduction of viral nucleic acid sequences and expression    constructs which bring about the degradation of RNA, or of an    expression cassette ensuring the expression of the former;-   h) introduction of constructs for inducing homologous recombination    on endogenous genes, for example for generating knockout mutants;-   i) introduction of mutations into endogenous genes for generating a    loss of function (e.g. generation of stop codons, reading-frame    shifts and the like); and/or-   j) identifying a non silent mutation e.g. generation of stop codons,    reading-frame shifts, inversions and the like in random mutagenized    population according to the so called tilling method.

Each of these methods may bring about a reduction in the expression, theactivity or the function for the purposes of the invention. A combineduse is also feasible. Further methods are known to the skilled workerand may encompass hindering or preventing processing of the protein,transport of the protein or its mRNA, inhibition of ribosomalattachment, inhibition of RNA splicing, induction of an enzyme whichdegrades RNA or the protein of the invention and/or inhibition oftranslational elongation or termination.

What follows is a brief description of the individual preferred methods:

A) Introduction of a Double-Stranded RNA Nucleic Acid Sequence (dsRNA)

The method of regulating genes by means of double-stranded RNA(“double-stranded RNA interference”; dsRNAi) has been describedextensively for animal, yeast, fungi and plant organisms such asNeurospora, Zebrafish, Drosophila, mice, planaria, humans, Trypanosoma,petunia or Arabidopsis (for example Matzke M A et al. (2000) Plant Mol.Biol. 43: 401-415; Fire A. et al. (1998) Nature 391: 806-811; WO99/32619; WO 99/53050; WO 00/68374; WO 00/44914; WO 00/44895; WO00/49035; WO 00/63364). In addition RNAi is also documented as anadvantageously tool for the repression of genes in bacteria such as E.coli for example by Tchurikov et al. [J. Biol. Chem., 2000, 275 (34):26523-26529]. Fire et al. named the phenomenon RNAi for RNAinterference. The techniques and methods described in the abovereferences are expressly referred to. Efficient gene suppression canalso be observed in the case of transient expression or followingtransient transformation, for example as the consequence of a biolistictransformation (Schweizer P et al. (2000) Plant J 2000 24: 895-903).dsRNAi methods are based on the phenomenon that the simultaneousintroduction of complementary strand and counterstrand of a genetranscript brings about highly effective suppression of the expressionof the gene in question. The resulting phenotype is very similar to thatof an analogous knock-out mutant (Waterhouse P M et al. (1998) Proc.Natl. Acad. Sci. USA 95: 13959-64).

Tuschl et al. [Gens Dev., 1999, 13 (24): 3191-3197] was able to showthat the efficiency of the RNAi method is a function of the length ofthe duplex, the length of the 3′-end overhangs, and the sequence inthese overhangs. Based on the work of Tuschl et al. and assuming thatthe underlining principles are conserved between different species thefollowing guidelines can be given to the skilled worker:

-   -   to achieve good results the 5′ and 3′ untranslated regions of        the used nucleic acid sequence and regions close to the start        codon should be in general avoided as this regions are richer in        regulatory protein binding sites and interactions between RNAi        sequences and such regulatory proteins might lead to undesired        interactions;    -   in plants the 5′ and 3′ untranslated regions of the used nucleic        acid sequence and regions close to the start codon preferably 50        to 100 nt upstream of the start codon give good results and        therefore should not be avoided;    -   preferably a region of the used mRNA is selected, which is 50 to        100 nt (=nucleotides or bases) downstream of the AUG start        codon;    -   only dsRNA (=double-stranded RNA) sequences from exons are        useful for the method, as sequences from introns have no effect;    -   the G/C content in this region should be greater than 30% and        less than 70% ideally around 50%;    -   a possible secondary structure of the target mRNA is less        important for the effect of the RNAi method.

The dsRNAi method has proved to be particularly effective andadvantageous for reducing the expression of the nucleic acid sequencesof the SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ IDNO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29,SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO:70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ IDNO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98,SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ IDNO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116,SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ IDNO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134,SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO:142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO:167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO:185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO:203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO:228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO:251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO:269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO:296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO:314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO:332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO:350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO:368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO:386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO:404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO:422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 and/or homologs thereof. Asdescribed inter alia in WO 99/32619, dsRNAi approaches are clearlysuperior to traditional antisense approaches.

The invention therefore furthermore relates to double-stranded RNAmolecules (dsRNA molecules) which, when introduced into an organism,advantageously into a plant (or a cell, tissue, organ or seed derivedtherefrom), bring about altered metabolic activity by the reduction inthe expression of the nucleic acid sequences of the SEQ ID NO: 1, SEQ IDNO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ IDNO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64,SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO:74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ IDNO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102,SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ IDNO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120,SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ IDNO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138,SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO:153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO:171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO:189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO:207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO:232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO:255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO:282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO:300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO:318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO:336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO:354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO:372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO:390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO:408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO:426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 orSEQ ID NO: 440 and/or homologs thereof. In a double-stranded RNAmolecule for reducing the expression of an protein encoded by a nucleicacid sequence of one of the SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5,SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15,SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO:27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ IDNO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86,SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO:96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO:114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO:132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155,SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ IDNO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173,SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ IDNO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191,SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ IDNO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209,SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ IDNO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234,SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ IDNO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257,SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ IDNO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284,SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ IDNO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302,SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ IDNO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320,SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ IDNO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338,SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ IDNO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356,SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ IDNO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374,SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ IDNO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392,SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ IDNO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410,SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ IDNO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428,SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 and/orhomologs thereof,

-   i) one of the two RNA strands is essentially identical to at least    part of a nucleic acid sequence, and-   ii) the respective other RNA strand is essentially identical to at    least part of the complementary strand of a nucleic acid sequence.

The term “essentially identical” refers to the fact that the dsRNAsequence may also include insertions, deletions and individual pointmutations in comparison to the target sequence while still bringingabout an effective reduction in expression. Preferably, the homology asdefined above amounts to at least 30%, preferably at least 40%, 50%,60%, 70% or 80%, very especially preferably at least 90%, mostpreferably 100%, between the “sense” strand of an inhibitory dsRNA and apart-segment of a nucleic acid sequence of the invention including in apreferred embodiment of the invention their endogenous 5′- and3′untranslated regions or between the “antisense” strand and thecomplementary strand of a nucleic acid sequence, respectively. Thepart-segment amounts to at least 10 bases, preferably at least 17, 18,19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 bases, especiallypreferably at least 40, 50, 60, 70, 80 or 90 bases, very especiallypreferably at least 100, 200, 300 or 400 bases, most preferably at least500, 600, 700, 800, 900 or more bases or at least 1000 or 2000 bases ormore in length. In another preferred embodiment of the invention thepart-segment amounts to 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 or 27bases, preferably to 20, 21, 22, 23, 24 or 25 bases. These shortsequences are preferred in animals and plants. The longer sequencespreferably between 200 and 800 bases are preferred in nonmammaliananimals, preferably in invertebrates, in yeast, fungi or bacteria, butthey are also useable in plants. Long double-stranded RNAs are processedin the organisms into many siRNAs (=small/short interfering RNAs) forexample by the protein Dicer, which is a ds-specific Rnase III enzyme.As an alternative, an “essentially identical” dsRNA may also be definedas a nucleic acid sequence, which is capable of hybridizing with part ofa gene transcript (for example in 400 mM NaCl, 40 mM PIPES pH 6.4, 1 mMEDTA at 50° C. or 70° C. for 12 to 16 h).

The dsRNA may consist of one or more strands of polymerizedribonucleotides. Modification of both the sugar-phosphate backbone andof the nucleosides may furthermore be present. For example, thephosphodiester bonds of the natural RNA can be modified in such a waythat they encompass at least one nitrogen or sulfur hetero atom. Basesmay undergo modification in such a way that the activity of, forexample, adenosine deaminase is restricted. These and othermodifications are described herein below in the methods for stabilizingantisense RNA.

The dsRNA can be prepared enzymatically; it may also be synthesizedchemically, either in full or in part. Short dsRNA up to 30 bp, whicheffectively mediate RNA interference, can be for example efficientlygenerated by partial digestion of long dsRNA templates using E. coliribonuclease III (RNase III). (Yang, D., et al. (2002) Proc. Natl. Acad.Sci. USA 99, 9942.)

The double-stranded structure can be formed starting from a single,self-complementary strand or starting from two complementary strands. Ina single, self-complementary strand, “sense” and “antisense” sequencecan be linked by a linking sequence (“linker”) and form for example ahairpin structure. Preferably, the linking sequence may take the form ofan intron, which is spliced out following dsRNA synthesis. The nucleicacid sequence encoding a dsRNA may contain further elements such as, forexample, transcription termination signals or polyadenylation signals.If the two strands of the dsRNA are to be combined in a cell or anorganism advantageously in a plant, this can be brought about in avariety of ways:

-   a) transformation of the cell or of the organism, advantageously of    a plant, with a vector encompassing the two expression cassettes;-   b) cotransformation of the cell or of the organism, advantageously    of a plant, with two vectors, one of which encompasses the    expression cassettes with the “sense” strand while the other    encompasses the expression cassettes with the “antisense” strand;-   c) supertransformation of the cell or of the organism,    advantageously of a plant, with a vector encompassing the expression    cassettes with the “sense” strand, after the cell or the organism    had already been transformed with a vector encompassing the    expression cassettes with the “antisense” strand;-   d) hybridization e.g. crossing of two organisms, advantageously of    plants, each of which has been transformed with one vector, one of    which encompasses the expression cassettes with the “sense” strand    while the other encompasses the expression cassettes with the    “antisense” strand;-   e) introduction of a construct comprising two promoters that lead to    transcription of the desired sequence from both directions; and/or-   f) infecting of the cell or of the organism with, advantageously of    a plant, with an engineered virus, which is able to produce the    desired dsRNA molecule.

Formation of the RNA duplex can be initiated either outside the cell orwithin the cell. If the dsRNA is synthesized outside the target cell ororganism it can be introduced into the organism or a cell of theorganism by injection, microinjection, electroporation, high velocityparticles, by laser beam or mediated by chemical compounds(DEAE-dextran, calciumphosphate, liposomes) or in case of animals it isalso possible to feed bacteria such as E. coli strains engineered toexpress double-stranded RNAi to the animals.

As shown in WO 99/53050, the dsRNA may also encompass a hairpinstructure, by linking the “sense” and “antisense” strands by a “linker”(for example an intron). The self-complementary dsRNA structures arepreferred since they merely require the expression of a construct andalways encompass the complementary strands in an equimolar ratio.

The expression cassettes encoding the “antisense” or the “sense” strandof the dsRNA or the self-complementary strand of the dsRNA arepreferably inserted into a vector and stably inserted into the genome ofa plant, using the methods described herein below (for example usingselection markers), in order to ensure permanent expression of thedsRNA. Transient expression with bacterial or viral vectors are similaruseful.

The dsRNA can be introduced using an amount which makes possible atleast one copy per cell. A larger amount (for example at least 5, 10,100, 500 or 1 000 copies per cell) may bring about more efficientreduction.

As has already been described, 100% sequence identity between the dsRNAand a gene transcript of a nucleic acid sequence of one of the SEQ IDNO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ IDNO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31;SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO:72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ IDNO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100,SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ IDNO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118,SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ IDNO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136,SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO:169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO:205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO:230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO:253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO:271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO:316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO:334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO:352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO:370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO:388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO:424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQID NO: 434 or SEQ ID NO: 440 or it's homolog is not necessarily requiredin order to bring about effective reduction in the expression. Theadvantage is, accordingly, that the method is tolerant with regard tosequence deviations as may be present as a consequence of geneticmutations, polymorphisms or evolutionary divergences. Thus, for example,using the dsRNA, which has been generated starting from a sequence ofone of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ IDNO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29,SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO:70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ IDNO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98,SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ IDNO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116,SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ IDNO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134,SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO:142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO:167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO:185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO:203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO:228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO:251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO:269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO:296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO:314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO:332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO:350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO:368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO:386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO:404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO:422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or homologs thereof of theone organism, may be used to suppress the corresponding expression inanother organism.

Due to the high degree of sequence homology between SEQ ID NO: 1, SEQ IDNO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ IDNO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64,SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO:74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ IDNO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102,SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ IDNO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120,SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ IDNO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138,SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO:153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO:171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO:189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO:207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO:232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO:255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO:282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO:300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO:318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO:336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO:354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO:372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO:390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO:408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO:426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 orSEQ ID NO: 440 from various organisms (e.g. plants), allows theconclusion that these proteins may be conserved to a high degree within,for example other plants, it is optionally possible so that theexpression of a dsRNA derived from one of the disclosed sequences asshown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ IDNO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29,SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO:70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ IDNO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98,SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ IDNO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116,SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ IDNO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134,SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO:142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO:167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO:185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO:203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO:228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO:251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO:269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO:296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO:314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO:332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO:350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO:368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO:386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO:404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO:422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or homologs thereof shouldalso have an advantageous effect in other plant species.

The dsRNA can be synthesized either in vivo or in vitro. To this end, aDNA sequence encoding a dsRNA can be introduced into an expressioncassette under the control of at least one genetic control element (suchas, for example, promoter, enhancer, silencer, splice donor or spliceacceptor or polyadenylation signal). Suitable advantageous constructsare described herein below. Polyadenylation is not required, nor doelements for initiating translation have to be present.

A dsRNA can be synthesized chemically or enzymatically. Cellular RNApolymerases or bacteriophage RNA polymerases (such as, for example T3,T7 or SP6 RNA polymerase) can be used for this purpose. Suitable methodsfor the in-vitro expression of RNA are described (WO 97/32016; U.S. Pat.Nos. 5,593,874; 5,698,425, 5,712,135, 5,789,214, 5,804,693). Prior tointroduction into a cell, tissue or organism, a dsRNA which has beensynthesized in vitro either chemically or enzymatically can be isolatedto a higher or lesser degree from the reaction mixture, for example byextraction, precipitation, electrophoresis, chromatography orcombinations of these methods. The dsRNA can be introduced directly intothe cell or else be applied extracellularly (for example into theinterstitial space). In one embodiment of the invention the RNAi methodleads to only a partial loss of gene function and therefore enables theskilled worker to study a gene dose effect in the disered organism andto fine tune the process of the invention. In another preferredembodiment it leads to a total loss of function and therefore increasesthe production of the fine chemical. Furthermore it enables a personskilled in the art to study multiple functions of a gene.

Stable transformation of the plant with an expression construct, whichbrings about the expression of the dsRNA is preferred, however. Suitablemethods are described herein below.

B) Introduction of an Antisense Nucleic Acid Sequence

Methods for suppressing a specific protein by preventing theaccumulation of its mRNA by means of “antisense” technology can be usedwidely and has been described extensively, including for plants [Sheehyet al. (1988) Proc. Natl. Acad. Sci. USA 85: 8805-8809; US 4,801,34100;Mol J N et al. (1990) FEBS Lett 268(2): 427-430]. The antisense nucleicacid molecule hybridizes with, or binds to, the cellular mRNA and/or thegenomic DNA encoding the target protein to be suppressed. This processsuppresses the transcription and/or translation of the target protein.Hybridization can be brought about in the conventional manner via theformation of a stable duplex or, in the case of genomic DNA, by theantisense nucleic acid molecule binding to the duplex of the genomic DNAby specific interaction in the large groove of the DNA helix.

An “antisense” nucleic acid molecule comprises a nucleotide sequence,which is at least in part complementary to a “sense” nucleic acidmolecule encoding a protein, e.g., complementary to the coding strand ofa double-stranded cDNA molecule or complementary to an encoding mRNAsequence. Accordingly, an antisense nucleic acid molecule can bind viahydrogen bonds to a sense nucleic acid molecule. The antisense nucleicacid molecule can be complementary to an entire coding strand of anucleic acid molecule conferring the expression of the polypeptide ofthe invention or to only a portion thereof. Accordingly, an antisensenucleic acid molecule can be antisense to a “coding region” of thecoding strand of a nucleotide sequence of a nucleic acid molecule of thepresent invention. Advantageously the noncoding region is in the area of50 bp, 100 bp, 200 bp or 300 bp, preferably 400 bp, 500 bp, 600 bp, 700bp, 800 bp, 900 bp or 1000 bp up- and/or downstream from the codingregion. The term “coding region” refers to the region of the nucleotidesequence comprising codons, which are translated into amino acidresidues. Further, the antisense nucleic acid molecule is antisense to a“noncoding region” of the mRNA flanking the coding region of anucleotide sequence. The term “noncoding region” refers to 5′ and 3′sequences which flank the coding region that are not translated into apolypeptide, i.e., also referred to as 5′ and 3′ untranslated regions(5′-UTR or 3′-UTR).

Given the coding strand sequences encoding the polypeptide of thepresent invention, e.g. having above mentioned activity, e.g. theactivity of a polypeptide with the biological activity of the protein ofthe invention as disclosed herein, antisense nucleic acid molecules ofthe invention can be designed according to the rules of Watson and Crickbase pairing.

An antisense nucleic acid sequence which is suitable for reducing theactivity of a protein can be deduced using the nucleic acid sequenceencoding this protein, for example the nucleic acid sequence as shown inSEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9,SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO:21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ IDNO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80,SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO:90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ IDNO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108,SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ IDNO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126,SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ IDNO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ IDNO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167,SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ IDNO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185,SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ IDNO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203,SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ IDNO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228,SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ IDNO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251,SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ IDNO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269,SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ IDNO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296,SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ IDNO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314,SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ IDNO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332,SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ IDNO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350,SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ IDNO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368,SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ IDNO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386,SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ IDNO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404,SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ IDNO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422,SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ IDNO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 (or homologs, analogs,paralogs, orthologs thereof), by applying the base-pair rules of Watsonand Crick. The antisense nucleic acid sequence can be complementary toall of the transcribed mRNA of the protein; it may be limited to thecoding region, or it may only consist of one oligonucleotide, which iscomplementary to part of the coding or noncoding sequence of the mRNA.Thus, for example, the oligonucleotide can be complementary to thenucleic acid region, which encompasses the translation start for theprotein. Antisense nucleic acid sequences may have an advantageouslength of, for example, 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50nucleotides but they may also be longer and encompass at least 100, 200,500, 1000, 2000 or 5000 nucleotides. A particular preferred length isbetween 15 and 30 nucleotides such as 15, 20, 25 or 30 nucleotides.Antisense nucleic acid sequences can be expressed recombinantly orsynthesized chemically or enzymatically using methods known to theskilled worker. For example, an anti-sense nucleic acid molecule (e.g.,an antisense oligonucleotide) can be chemically synthesized usingnaturally occurring nucleotides or variously modified nucleotidesdesigned to increase the biological stability of the molecules or toincrease the physical stability of the duplex formed between theantisense and sense nucleic acids, e.g., phosphorothioate derivativesand acridine substituted nucleotides can be used. Examples of substanceswhich can be used are phosphorothioate derivatives andacridine-substituted nucleotides such as 5-fluorouracil, 5-bromouracil,5-chlorouracil, 5-iodouracil, hypoxanthin, xanthin, 4-acetylcytosine,5-(carboxyhydroxymethyl)uracil,5-carboxymethylaminomethyl-2-thiouridine,5-carboxymethylaminomethyluracil, dihydrouracil, β-D-galactosylqueosine,inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine,2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine,5-methylcytosine, N6-adenine, 7-methylguanine,5-methylaminomethyluracil, 5-methoxyaminomethyl-2-thiouracil,β-D-mannosylqueosine, 5′-methoxycarboxymethyluracil, 5-methoxyuracil,2-methylthio-N6-isopentenyladenine, uracil-5-oxyacetic acid,pseudouracil, queosine, 2-thiocytosine, 5-methyl-2-thiouracil,2-thiouracil, 4-thiouracil, 5-methyluracil, methyl uracil-5-oxyacetate,uracil-5-oxyacetic acid, 5-methyl-2-thiouracil,3-(3-amino-3-N-2-carboxypropyl)uracil and 2,6-diaminopurine.Alternatively, the antisense nucleic acid can be produced biologicallyusing an expression vector into which a nucleic acid molecule has beensubcloned in an anti-sense orientation (i.e., RNA transcribed from theinserted nucleic acid molecule will be of an antisense orientation to atarget nucleic acid molecule of interest, described further in thefollowing subsection).

In a further preferred embodiment, the expression of a protein encodedby one of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ IDNO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30,SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ IDNO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99,SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ IDNO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117,SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ IDNO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ IDNO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160,SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ IDNO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178,SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ IDNO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196,SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ IDNO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214,SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ IDNO: 231, SEQ ID NO: 233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239,SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ IDNO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262,SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ IDNO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289,SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ IDNO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307,SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ IDNO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325,SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ IDNO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343,SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ IDNO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361,SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ IDNO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379,SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ IDNO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397,SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ IDNO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415,SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ IDNO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433,SEQ ID NO: 435 or SEQ ID NO: 441 or homologs, analogs, paralogs,orthologs thereof can be inhibited by nucleotide sequences which arecomplementary to the regulatory region of a gene (for example a promoterand/or enhancer) and which may form triplex structures with the DNAdouble helix in this region so that the transcription of the gene isreduced. Such methods have been described (Helene C (1991) AnticancerDrug Res. 6(6): 569-84; Helene C et al. (1992) Ann. NY Acad. Sci. 660:27-36; Maher L J (1992) Bioassays 14(12): 807-815).

In a further embodiment, the antisense nucleic acid molecule can be anα-anomeric nucleic acid. Such α-anomeric nucleic acid molecules formspecific double-stranded hybrids with complementary RNA in which—asopposed to the conventional β-nucleic acids—the two strands run inparallel with one another (Gautier C et al. (1987) Nucleic Acids Res.15: 6625-6641). Furthermore, the antisense nucleic acid molecule canalso comprise 2′-O-methylribonucleotides [Inoue et al. (1987) NucleicAcids Res. 15: 6131-6148] or chimeric RNA-DNA analogs [Inoue et al.(1987) FEBS Lett 215: 327-330].

The antisense nucleic acid molecules of the invention are typicallyadministered to a cell or generated in situ such that they hybridizewith or bind to cellular mRNA and/or genomic DNA encoding a polypeptidehaving the biological activity of protein of the invention therebyinhibit expression of the protein, e.g., by inhibiting transcriptionand/or translation and leading to the aforementioned fine chemicalincreasing activity.

The antisense molecule of the present invention comprises also a nucleicacid molecule comprising a nucleotide sequences complementary to theregulatory region of an nucleotide sequence encoding the naturaloccurring polypeptide of the invention, e.g. the polypeptide sequencesshown in the sequence listing, or identified according to the methodsdescribed herein, e.g., its promoter and/or enhancers, e.g. to formtriple helical structures that prevent transcription of the gene intarget cells. See generally, Helene, C. (1991) Anticancer Drug Des.6(6): 569-84; Helene, C. et al. (1992) Ann. N.Y. Acad. Sci. 660:27-36;and Maher, L. J. (1992) Bioassays 14(12): 807-15.

C) Introduction of an Antisense Nucleic Acid Sequence Combined with aRibozyme

It is advantageous to combine the above-described antisense strategywith a ribozyme method. Catalytic RNA molecules or ribozymes can beadapted to any target RNA and cleave the phosphodiester backbone atspecific positions, thus functionally deactivating the target RNA(Tanner N K (1999) FEMS Microbiol. Rev. 23(3): 257-275). The ribozymeper se is not modified thereby, but is capable of cleaving furthertarget RNA molecules in an analogous manner, thus acquiring theproperties of an enzyme. The incorporation of ribozyme sequences into“antisense” RNAs imparts this enzyme-like RNA-cleaving property toprecisely these “antisense” RNAs and thus increases their efficiencywhen inactivating the target RNA. The preparation and the use ofsuitable ribozyme “antisense” RNA molecules is described, for example,by Haseloff et al. (1988) Nature 33410: 585-591.

Further the antisense nucleic acid molecule of the invention can be alsoa ribozyme. Ribozymes are catalytic RNA molecules with ribonucleaseactivity, which are capable of cleaving a single-stranded nucleic acid,such as an mRNA, to which they have a complementary region. In thismanner, ribozymes [for example “Hammerhead” ribozymes; Haselhoff andGerlach (1988) Nature 33410: 585-591] can be used to catalyticallycleave the mRNA of an enzyme to be suppressed and to preventtranslation. The ribozyme technology can increase the efficacy of ananti-sense strategy. Methods for expressing ribozymes for reducingspecific proteins are described in (EP 0 291 533, EP 0 321 201, EP 0 360257). Ribozyme expression has also been described for plant cells(Steinecke P et al. (1992) EMBO J 11(4): 1525-1530; de Feyter R et al.(1996) Mol. Gen. Genet. 250(3): 329-338). Suitable target sequences andribozymes can be identified for example as described by Steinecke P,Ribozymes, Methods in Cell Biology 50, Galbraith et al. eds, AcademicPress, Inc. (1995), pp. 449-460 by calculating the secondary structuresof ribozyme RNA and target RNA and by their interaction [Bayley C C etal. (1992) Plant Mol. Biol. 18(2): 353-361; Lloyd A M and Davis R W etal. (1994) Mol. Gen. Genet. 242(6): 653-657]. For example, derivativesof the tetrahymena L-19 IVS RNA, which have complementary regions to themRNA of the protein to be suppressed can be constructed (see also U.S.Pat. No. 4,987,071 and U.S. Pat. No. 5,116,742). As an alternative, suchribozymes can also be identified from a library of a variety ofribozymes via a selection process (Bartel D and Szostak J W (1993)Science 261: 1411-1418).

D) Introduction of a (Sense) Nucleic Acid Sequence for InducingCosuppression

The expression of a nucleic acid sequence in sense orientation can leadto cosuppression of the corresponding homologous, endogenous genes. Theexpression of sense RNA with homology to an endogenous gene can reduceor indeed eliminate the expression of the endogenous gene, in a similarmanner as has been described for the following antisense approaches:Jorgensen et al. [(1996) Plant Mol. Biol. 31(5): 957-973], Goring et al.[(1991) Proc. Natl. Acad. Sci. USA 88: 1770-1774], Smith et al. [(1990)Mol. Gen. Genet. 224: 447-481], Napoli et al. [(1990) Plant Cell 2:279-289] or Van der Krol et al. [(1990) Plant Cell 2: 291-99]. In thiscontext, the construct introduced may represent the homologous gene tobe reduced either in full or only in part. The application of thistechnique to plants has been described for example by Napoli et al.[(1990) The Plant Cell 2: 279-289 and in US 5,03410,323]. Furthermorethe above described cosuppression strategy can advantageously becombined with the RNAi method as described by Brummell et al., 2003,Plant J. 33, pp 793-800.

E) Introduction of Nucleic Acid Sequences Encoding a Dominant-NegativeProtein

The function or activity of a protein can efficiently also be reduced byexpressing a dominant-negative variant of said protein. The skilledworker is familiar with methods for reducing the function or activity ofa protein by means of coexpression of its dominant-negative form [LagnaG and Hemmati-Brivanlou A (1998) Current Topics in Developmental Biology36: 75-98; Perlmutter R M and Alberola-IIa J (1996) Current Opinion inImmunology 8(2): 285-90; Sheppard D (1994) American Journal ofRespiratory Cell & Molecular Biology 11(1): 1-6; Herskowitz I (1987)Nature 329 (6136): 219-22].

A dominant-negative variant can be realized for example by changing ofan amino acid in the proteins encoded by one of SEQ ID NO: 2, SEQ ID NO:4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ IDNO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75,SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO:85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ IDNO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103,SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ IDNO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ IDNO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139,SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ IDNO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ IDNO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182,SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ IDNO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ IDNO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO: 231, SEQ ID NO: 233, SEQ IDNO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243,SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ IDNO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266,SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ IDNO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293,SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ IDNO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311,SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ IDNO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329,SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ IDNO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347,SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ IDNO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365,SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ IDNO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 393,SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ IDNO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ IDNO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419,SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ IDNO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:441 or homologs thereof. This change can be determined for example bycomputer-aided comparison (“alignment”). These mutations for achieving adominant-negative variant are preferably carried out at the level of thenucleic acid sequences. A corresponding mutation can be performed forexample by PCR-mediated in-vitro mutagenesis using suitableoligonucleotide primers by means of which the desired mutation isintroduced. To this end, methods are used with which the skilled workeris familiar. For example, the “LA PCR in vitro Mutagenesis Kit” (TakaraShuzo, Kyoto) can be used for this purpose. It is also possible andknown to those skilled in the art that deleting or changing offunctional do mains, e.g. TF or other signaling components which canbind but not activate may achieve the reduction of protein activity.

F) Introduction of DNA- or Protein-Binding Factors Against Genes, RNAsor Proteins

A reduction in the expression of a gene encoded by one of SEQ ID NO: 1,SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11,SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO:23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ IDNO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82,SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO:92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ IDNO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ IDNO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128,SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ IDNO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151,SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ IDNO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169,SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ IDNO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187,SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ IDNO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205,SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ IDNO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ IDNO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253,SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ IDNO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271,SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ IDNO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298,SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ IDNO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ IDNO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334,SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ IDNO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ IDNO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370,SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ IDNO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388,SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ IDNO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406,SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ IDNO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424,SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ IDNO: 434 or SEQ ID NO: 440 or homologs thereof according to the inventioncan also be achieved with specific DNA-binding factors, for examplefactors of the zinc finger transcription factor type. These factorsattach to the genomic sequence of the endogenous target gene, preferablyin the regulatory regions, and bring about repression of the endogenousgene. The use of such a method makes possible the reduction in theexpression of an endogenous gene without it being necessary torecombinantly manipulate the sequence of the latter. Such methods forthe preparation of relevant factors are described in Dreier B et al.[(2001) J. Biol. Chem. 276(31): 29466-78 and (2000) J. Mol. Biol.303(4): 489-502], Beerli R R et al. [(1998) Proc. Natl. Acad. Sci. USA95(25): 14628-14633; (2000) Proc. Natl. Acad. Sci. USA 97(4): 1495-1500and (2000) J. Biol. Chem. 275(42): 32617-32627)], Segal D J and Barbas CF [3rd (2000) Curr. Opin. Chem. Biol. 4(1): 3410-39], Kang JS and Kim JS[(2000) J. Biol. Chem. 275(12): 8742-8748], Kim J S et al. [(1997) Proc.Natl. Acad. Sci. USA 94(8): 3616-3620], Klug A [(1999) J. Mol. Biol.293(2): 215-218], Tsai S Y et al. [(1998) Adv. Drug Deliv. Rev. 30(1-3):23-31], Mapp A K et al. [(2000) Proc. Natl. Acad. Sci. USA 97(8):3930-3935], Sharrocks A D et al. [(1997) Int. J. Biochem. Cell Biol.29(12): 1371-1387] and Zhang L et al. [(2000) J. Biol. Chem. 275(43):33850-33860]. Examples for the application of this technology in plantshave been described in WO 01/52620, Ordiz M I et al., (Proc. Natl. Acad.Sci. USA, Vol. 99, Issue 20, 13290-13295, 2002) or Guan et al., (Proc.Natl. Acad. Sci. USA, Vol. 99, Issue 20, 13296-13301, 2002)

These factors can be selected using any portion of a gene. This segmentis preferably located in the promoter region. For the purposes of genesuppression, however, it may also be located in the region of the codingexons or introns. The skilled worker can obtain the relevant segmentsfrom Genbank by database search or starting from a cDNA whose gene isnot present in Genbank by screening a genomic library for correspondinggenomic clones.

It is also possible to first identify sequences in a target crop, whichare encoded by one of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ IDNO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ IDNO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68,SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO:78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ IDNO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO:106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO:124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: ,SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ IDNO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165,SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ IDNO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ IDNO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201,SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ IDNO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ IDNO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249,SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ IDNO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267,SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ IDNO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ IDNO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ IDNO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330,SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ IDNO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348,SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ IDNO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366,SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ IDNO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ IDNO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402,SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ IDNO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ IDNO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or homologsthereof, then find the promoter and reduce expression by the use of theabovementioned factors.

The skilled worker is familiar with the methods required for doing so.

Furthermore, factors which are introduced into a cell may also be thosewhich themselves inhibit the target protein. The protein-binding factorscan, for example, be aptamers [Famulok M and Mayer G (1999) Curr. TopMicrobiol. Immunol. 243: 123-36] or antibodies or antibody fragments orsingle-chain anti bodies. Obtaining these factors has been described,and the skilled worker is familiar therewith. For example, a cytoplasmicscFv antibody has been employed for modulating activity of thephytochrome A protein in genetically modified tobacco plants [Owen M etal (1992) Biotechnology (NY) 10(7): 790-794; Franken E et al. (1997)Curr. Opin. Biotechnol. 8(4): 411-416; Whitelam (1996) Trend Plant Sci.1: 286-272].

Gene expression may also be suppressed by tailor-madelow-molecular-weight synthetic compounds, for example of the polyamidetype [Dervan P B and Bürli R W (1999) Current Opinion in ChemicalBiology 3: 688-693; Gottesfeld J M et al. (2000) Gene Expr. 9(1-2):77-91]. These oligomers consist of the units3-(dimethylamino)propylamine, N-methyl-3-hydroxypyrrole,N-methylimidazole and N-methylpyrroles; they can be adapted to eachportion of double-stranded DNA in such a way that they bindsequence-specifically to the large groove and block the expression ofthe gene sequences located in this position. Suitable methods have beendescribed in Bremer R E et al. [(2001) Bioorg. Med. Chem. 9(8):2093-103], Ansari A Z et al. [(2001) Chem. Biol. 8(6): 583-92],Gottesfeld J M et al. [(2001) J. Mol. Biol. 309(3): 615-29], Wurtz N Ret al. [(2001) Org. Lett 3(8): 1201-3], Wang C C et al. [(2001) Bioorg.Med. Chem. 9(3): 653-7], Urbach A R and Dervan P B [(2001) Proc. Natl.Acad. Sci. USA 98(8): 434103-8] and Chiang S Y et al. [(2000) J. Biol.Chem. 275(32): 24246-54].

G) Introduction of Viral Nucleic Acid Sequences and ExpressionConstructs which Bring about the Degradation of RNA

Inactivation or downregulation can also be efficiently brought about byinducing specific RNA degradation by the organism, advantageously in theplant, with the aid of a viral expression system (Amplikon) [Angell, S Met al. (1999) Plant J. 20(3): 357-362]. Nucleic acid sequences withhomology to the transcripts to be suppressed are introduced into theplant by these systems—also referred to as “VIGS” (viral induced genesilencing) with the aid of viral vectors. Then, transcription isswitched off, presumably mediated by plant defense mechanisms againstviruses. Suitable techniques and methods are described in Ratcliff F etal. [(2001) Plant J. 25(2): 237-45], Fagard M and Vaucheret H [(2000)Plant Mol. Biol. 43(2-3): 285-93], Anandalakshmi R et al. [(1998) Proc.Natl. Acad. Sci. USA 95(22): 13079-84] and Ruiz M T [(1998) Plant Cell10(6): 937-46].

H) Introduction of Constructs for Inducing a Homologous Recombination onEndogenous Genes, for Example for Generating Knock-Out Mutants

To generate a homologously-recombinant organism with reduced activity, anucleic acid construct is used which, for example, comprises at leastpart of an endogenous gene which is modified by a deletion, addition orsubstitution of at least one nucleotide in such a way that thefunctionality is reduced or completely eliminated. The modification mayalso affect the regulatory elements (for example the promoter) of thegene so that the coding sequence remains unmodified, but expression(transcription and/or translation) does not take place or is reduced.

In the case of conventional homologous recombination, the modifiedregion is flanked at its 5′ and 3′ end by further nucleic acidsequences, which must be sufficiently long for allowing recombination.Their length is, as a rule, in a range of from one hundred bases up toseveral kilobases [Thomas K R and Capecchi M R (1987) Cell 51: 503;Strepp et al. (1998) Proc. Natl. Acad. Sci. USA 95(8): 4368-4373]. Inthe case of homologous recombination, the host organism—for example aplant—is transformed with the recombination construct using the methodsdescribed herein below, and clones, which have successfully undergonerecombination are selected using for example a resistance to antibioticsor herbicides. Using the cotransformation technique, the resistance toantibiotics or herbicides can subsequently advantageously bere-eliminated by performing crosses. An example for an efficienthomologous recombination system in plants has been published in Nat.Biotechnol. 2002 October; 20(10): 1030-4, Terada R et al.: Efficientgene targeting by homologous recombination in rice.

Homologous recombination is a relatively rare event in highereukaryotes, especially in plants. Random integrations into the hostgenome predominate. One possibility of removing the randomly integratedsequences and thus increasing the number of cell clones with a correcthomologous recombination is the use of a sequence-specific recombinationsystem as described in U.S. Pat. No. 6,110,736, by means of whichunspecifically integrated sequences can be deleted again, whichsimplifies the selection of events which have integrated successfullyvia homologous recombination. A multiplicity of sequence-specificrecombination systems may be used, examples which may be mentioned beingCre/lox system of bacteriophage P1, the FLP/FRT system from yeast, theGin recombinase of phage Mu, the Pin recombinase from E. coli and theR/RS system of the pSR1 plasmid. The bacteriophage P1 Cre/lox system andthe yeast FLP/FRT system are preferred. The FLP/FRT and the cre/loxrecombinase system have already been applied to plant systems [Odell etal. (1990) Mol. Gen. Genet. 223: 369-378].

I) Introduction of Mutations into Endogenous Genes for Bringing About aLoss of Function (For Example Generation of Stop Codons, Reading-FrameShifts and the Like)

Further suitable methods for reducing activity are the introduction ofnonsense mutations into endogenous genes, for example by introducingRNA/DNA oligonucleotides into the plant [Zhu et al. (2000) Nat.Biotechnol. 18(5): 555-558], and the generation of knock-out mutantswith the aid of, for example, T-DNA mutagenesis [Koncz et al. (1992)Plant Mol. Biol. 20(5): 963-976],ENU-(N-ethyl-N-nitrosourea)—mutagenesis or homologous recombination[Hohn B and Puchta (1999) H. Proc. Natl. Acad. Sci. USA 96: 8321-8323].Point mutations may also be generated by means of DNA-RNA hybrids alsoknown as “chimeraplasty” [Cole-Strauss et al. (1999) Nucl. Acids Res.27(5): 1323-1330; Kmiec (1999) Gene Therapy American Scientist 87(3):240-247]. The mutation sites may be specifically targeted or randomlyselected. If the mutations have been created randomly e.g. byTransposon-Tagging or chemical mutagenesis, the skilled worked is ableto specifically enrich selected mutation events in the inventive nucleicacids.

Nucleic acid sequences as described in item B) to I) are expressed inthe cell or organism by transformation/transfection of the cell ororganism or are introduced in the cell or organism by known methods, forexample as disclosed in item A).

In one further embodiment of the process according to the invention,organisms are used in which one of the abovementioned genes, or one ofthe above-mentioned nucleic acids, is mutated in such a manner that theactivity of the encoded gene products is influenced by cellular factorsto a greater extent than in the reference organism, as compared with theunmutated proteins. This kind of mutation could lead to a change in themetabolic activity of the organism, which than causes in a higherproduction of the fine chemical. The reason for this higher productivitycan be due to a change in regulation mechanism of enzymic activity suchas substrate inhibition or feed back regulation. In a further embodimentthe process according to the invention, organisms are grown under suchconditions, that the expression of the nucleic acids of the invention isreduced or repressed leading to an enhanced production of the finechemical according to the invention.

Owing to the reduction or deletion of a gene or a plurality of genesconferring the expression of the nucleic acid molecule of the inventionor the polypeptide of the invention, for example the nucleic acidconstruct mentioned below, into an organism alone or in combination withother genes, it is possible not only to increase the biosynthetic fluxtowards the end product, but also to increase, modify or create de novoan advantageous, preferably novel metabolites composition in theorganism, e.g. an advantageous amino acid composition comprising ahigher content of from a viewpoint of nutritional physiology limitedamino acids, like methionine or lysine combined with higher amounts ofmetabolites positively affecting or lower amounts of metabolitesnegatively affecting the nutrition or health of animals or humansprovided with said compositions or organisms of the invention or partsthereof. Likewise, the number or activity of further genes which arerequired for the import or export of nutrients or metabolites, includingamino acids or its precursors, required for the cell's biosynthesis ofamino acids may be increased so that the concentration of necessary orrelevant precursors, cofactors or intermediates within the cell(s) orwithin the corresponding storage compartments is increased. Owing to thereduced, decreased or deleted activity of the polypeptide of theinvention or owing to the reduced or decreased number of nucleic acidsequences of the invention and/or to the modulation of further geneswhich are involved in the biosynthesis of the amino acids, e.g. byincreasing the activity of enzymes synthesizing precursors or bydestroying the activity of one or more genes which are involved in thebreakdown of the amino acids, it is possible to increase the yield,production and/or production efficiency of amino acids in the hostorganism, such as the plants or the microorganisms.

By influencing the metabolism thus, it is possible to produce, in theprocess according to the invention, further advantageoussulfur-containing compounds, which contain at least one sulfur atombound covalently. Examples of such compounds are, in addition tomethionine, homocysteine, S-adenosylmethionine, cysteine, advantageouslymethionine and S-adenosylmethionine.

Accordingly, in one embodiment, the process according to the inventionrelates to a process which comprises

-   a) providing a non-human organism, preferably a microorganism,    non-human animal, a plant cell, a plant tissue or a plant;-   b) reducing, decreasing or deleting the activity in the organism,    preferably in the microorganism, the non-human animal, the plant    cell, the plant tissue or the plant, of a protein having the    biological activity of the protein of the invention or being encoded    by the nucleic acid molecule of the present invention and described    below, e.g. having above mentioned activity, e.g. conferring an    increase of the fine chemical;-   c) growing the organism, preferably the microorganism, non-human    animal, the plant cell, the plant tissue or the plant under    conditions which permit the production of the fine chemical in the    organism, preferably the microorganism, the plant cell, the plant    tissue or the plant; and-   d) if desired, recovering, optionally isolating, the free and/or    bound methionine and, optionally further free and/or bound amino    acids synthesized by the organism, the microorganism, the non-human    animal, the plant or animal cell, the plant or animal tissue or the    plant.

The organism, in particular the microorganism, non-human animal, theplant or animal cell, the plant or animal tissue or the plant isadvantageously grown in such a manner that it is not only possible torecover, if desired isolate the free or bound methionine or the free andbound methionine but as option it is also possible to produce, recoverand, if desired isolate, other free or/and bound amino acids, inparticular methionine. Galili et al., Transgenic Res., 200, 9, 2,137-144 describes that the heterologous expression of a bacterial genefor the amino acid biosynthesis confers the increase of free as well asof protein-bound amino acids.

After the above-described reducing, decreasing or deleting (which asdefined above also encompasses the generating of an activity in anorganism, i.e. a de novo activity), for example after the introductionand the expression of the an RNAi molecule, antisense molecule orribozyme described in the methods or processes according to theinvention, the organism according to the invention, advantageously, amicroorganism, a non-human animal, a plant, plant or animal tissue orplant or animal cell, is grown and subsequently harvested.

Suitable organisms or host organisms (transgenic organism) for thenucleic acid molecule used according to the invention and for theinventive process, the nucleic acid construct or the vector (both asdescribed below) are, in principle, all organisms which are capable ofsynthesizing the fine chemical, and which are suitable for therepression, reduction or deletion of recombinant genes. Examples whichmay be mentioned are transgenic plants, cells or protoplasts thereof,transgenic microorganisms such as fungi, bacteria, yeasts, alga ordiatom, cells or protoplasts thereof. Preferred organisms are thosewhich are naturally capable of synthesizing the fine chemical insubstantial amounts, like fungi, yeasts, bacteria or plants. Inprinciple, transgenic animals, for example Caenorhabditis elegans, arealso suitable as host organisms.

In the event that the transgenic organism is a microorganism, such as aeukaryotic organism, for example a fungus, an alga, diatom or a yeast inparticular a fungus, alga, diatom or yeast selected from the familiesChaetomiaceae, Choanephoraceae, Cryptococcaceae, Cunninghamellaceae,Demetiaceae, Moniliaceae, Mortierellaceae, Mucoraceae, Pythiaceae,Sacharomycetaceae, Saprolegniaceae, Schizosacharomycetaceae,Sodariaceae, Sporobolomycetaceae Tuberculariaceae, Adelotheciaceae,Dinophyceae, Ditrichaceae or Prasinophyceae, or a prokaryotic organism,for example a bacterium or blue alga, in particular a bacterium from thefamilies Actinomycetaceae, Bacillaceae, Brevibacteriaceae,Corynebacteriaceae, Enterobacteriacae, Gordoniaceae, Nocardiaceae,Micrococcaceae, Mycobacteriaceae, Pseudomonaceae, Rhizobiaceae orStreptomycetaceae, this microorganism is grown on a solid or in a liquidmedium which is known to the skilled worker and suits the organism.After the growing phase, the organisms can be harvested. Themicroorganisms or the recovered, and if desired isolated, amino acidscan then be processed further directly into foodstuffs or animal feedsor for other applications, for example according to the disclosures madein EP-B-O 533 039 or EP-A-0 615 693, which are expressly incorporatedherein by reference. The fermentation broth or fermentation products canbe purified in the customary manner by extraction and precipitation orvia ion exchangers and other methods known to the person skilled in theart and described herein below. Products of these different work-upprocedures are amino acids or amino acid compositions which stillcomprise fermentation broth and cell components in different amounts,advantageously in the range of from 0 to 99% by weight, preferably below80% by weight, especially preferably between below 50% by weight.

Preferred microorganisms are selected from the group consisting ofChaetomiaceae such as the genera Chaetomium e.g. the speciesChaetomidium fimeti; Choanephoraceae such as the genera Blakeslea,Choanephora e.g. the species Blakeslea trispora, Choanephoracucurbitarum or Choanephora infundibulifera var. cucurbitarum;Cryptococcaceae such as the genera Candida, Crytococcus, Rhodotorula,Torulopsis e.g. the species Candida albicans, Candida albomarginata,Candida antarctica, Candida bacarum, Candida bogoriensis, Candidaboidinii, Candida bovina, Candida brumptii, Candida cacaoi, Candidacariosilignicola, Candida catenulata, Candida chalmersii, Candidacifemmi, Candida cylindracea, Candida edax, Candida ernobii, Candidafamata, Candida freyschussii, Candida friedrichii, Candida glabrata,Candida guilliermondii, Candida haemulonii, Candida humicola, Candidainconspicua, Candida ingens, Candida intermedia, Candida kefyr, Candidakrusei, Candida lactiscondensi, Candida lambica, Candida lipolytica,Candida lusitaniae, Candida macedoniensis, Candida magnoliae, Candidamembranaefaciens, Candida mesenterica, Candida multigemmis, Candidamycoderma, Candida nemodendra, Candida nitratophila, Candidanorvegensis, Candida norvegica, Candida parapsilosis, Candidapelliculosa, Candida peltata, Candida pini, Candida pseudotropicalis,Candida pulcherrima, Candida punicea, Candida pustula, Candida ravautii,Candida reukaufii, Candida rugosa, Candida sake, Candida silvicola,Candida solani, Candida sp., Candida spandovensis, Candida succiphila,Candida tropicalis, Candida utilis, Candida valida, Candida versatilis,Candida vini, Candida zeylanoides, Cryptococcus albidus, Cryptococcuscurvatus, Cryptococcus flavus, Cryptococcus humicola, Cryptococcushungaricus, Cryptococcus kuetzingii, Cryptococcus laurentii,Cryptococcus macerans, Cryptococcus neoformans, Cryptococcus terreus,Cryptococcus uniguttulatus, Rhodotorula acheniorum, Rhodotorula bacarum,Rhodotorula bogoriensis, Rhodotorula flava, Rhodotorula glutinis,Rhodotorula macerans, Rhodotorula minuta, Rhodotorula mucilaginosa,Rhodotorula pilimanae, Rhodotorula pustula, Rhodotorula rubra,Rhodotorula tokyoensis, Torulopsis colliculosa, Torulopsis dattila orTorulopsis neoformans; Cunninghamellaceae such as the generaCunninghamella e.g. the species Cunninghamella blakesleeana,Cunninghamella echinulata, Cunninghamella echinulata var. elegans,Cunninghamella elegans or Cunninghamella homothallica; Demetiaceae suchas the genera Alternaria, Bipolaris, Cercospora, Chalara, Cladosporium,Curvularia, Exophilia, Helicosporium, Helminthosporium, Orbimyces,Philalophora, Pithomyces, Spilocaea, Thielaviopsis, Wangiella e.g. thespecies Curvularia affinis, Curvularia clavata, Curvularia fallax,Curvularia inaequalis, Curvularia indica, Curvularia lunata, Curvulariapallescens, Curvularia verruculosa or Helminothosporium sp.; Moniliaceaesuch as the genera Arthrobotrys, Aspergillus, Epidermophyton,Geotrichum, Gliocladium, Histoplasma, Microsporum, Monilia,Oedocephalum, Oidium, Penicillium, Trichoderma, Trichophyton,Thrichoteclum, Verticillium e.g. the species Aspergillus aculeatus,Aspergillus albus, Aspergillus alliaceus, Aspergillus asperescens,Aspergillus awamori, Aspergillus candidus, Aspergillus carbonarius,Aspergillus carneus, Aspergillus chevalieri, Aspergillus chevalieri var.intermedius, Aspergillus clavatus, Aspergillus ficuum, Aspergillusflavipes, Aspergillus flavus, Aspergillus foetidus, Aspergillusfumigatus, Aspergillus giganteus, Aspergillus humicola, Aspergillusintermedius, Aspergillusjaponicus, Aspergillus nidulans, Aspergillusniger, Aspergillus niveus, Aspergillus ochraceus, Aspergillus oryzae,Aspergillus ostianus, Aspergillus parasiticus, Aspergillus parasiticusvar. globosus, Aspergillus penicillioides, Aspergillus phoenicis,Aspergillus rugulosus, Aspergillus sclerotiorum, Aspergillus sojae var.gymnosardae, Aspergillus sydowi, Aspergillus tamarii, Aspergillusterreus, Aspergillus terricola, Aspergillus toxicarius, Aspergillusunguis, Aspergillus ustus, Aspergillus versicolor, Aspergillusvitricolae, Aspergillus wentii, •Penicillium adametzi, Penicilliumalbicans, Penicillium arabicum, Penicillium arenicola, Penicilliumargillaceum, Penicillium arvense, Penicillium asperosporum, •Penicilliumaurantiogriseum, Penicillium avellaneum, •Penicillium baarnense,•Penicillium bacillisporum, Penicillium brasilianum, •Penicilliumbrevicompactum, •Penicillium camemberti, •Penicillium canadense,•Penicillium canescens, •Penicillium caperatum, •Penicillium capsulatum,Penicillium caseicolum, •Penicillium chrysogenum, •Penicilliumcitreonigrum, •Penicillium citrinum, •Penicillium claviforme,•Penicillium commune, •Penicillium corylophilum, Penicilliumcorymbiferum, •Penicillium crustosum, •Penicillium cyclopium,•Penicillium daleae, •Penicillium decumbens, •Penicillium dierckxii,•Penicillium digitatum, •Penicillium digitatum var. latum, •Penicilliumdivaricatum, •Penicillium diversum, •Penicillium duclauxii, Penicilliumechinosporum, •Penicillium expansum, •Penicillium fellutanum,Penicillium frequentans, •Penicillium funiculosum, •Penicillium glabrum,•Penicillium gladioli, •Penicillium griseofulvum, •Penicillium hirsutum,•Penicillium hispanicum, Penicillium islandicum, •Penicillium italicum,•Penicillium italicum var. avellaneum, Penicillium janczewskii,•Penicillium janthinellum, •Penicillium japonicum, •Penicilliumlavendulum, •Penicillium lilacinum, •Penicillium lividum, •Penicilliummartensii, Penicillium megasporum, •Penicillium miczynskii, •Penicilliumnalgiovense, •Penicillium nigricans, Penicillium notatum, •Penicilliumochrochloron, •Penicillium odoratum, •Penicillium oxalicum Penicilliumparaherquei, •Penicillium patulum, •Penicillium pinophilum, Penicilliumpiscarium, •Penicillium pseudostromaticum, •Penicillium puberulumPenicillium purpurogenum, •Penicillium raciborskii, •Penicilliumroqueforti, •Penicillium rotundum, •Penicillium rubrum, •Penicilliumsacculum, •Penicillium simplicissimum, Penicillium sp., Penicilliumspinulosum, Penicillium steckii, Penicillium stoloniferum, Penicilliumstriatisporum, Penicillium striatum, Penicillium tardum, Penicilliumthomii, Penicillium turbatum, Penicillium variabile, Penicilliumvermiculatum, Penicillium vermoesenii, Penicillium verrucosum,Penicillium verrucosum var. corymbiferum, Penicillium verrucosum var.cyclopium, Penicillium verruculosum, Penicillium vinaceum, Penicilliumviolaceum, Penicillium viridicatum, Penicillium vulpinum, Trichodermahamatum, Trichoderma harzianum, Trichoderma koningii, Trichodermalongibrachiatum, Trichoderma polysporum, Trichoderma reesei, Trichodermavirens or Trichoderma viride; Mortierellaceae such as the generaMortierella e.g. the species Mortierella isabellina, Mortierellapolycephala, Mortierella ramanniana, Mortierella vinacea or Mortierellazonata; Mucoraceae such as the genera Actinomucor, Mucor, Phycomyces,Rhizopus, Zygorhynchus e.g. the species Mucor amphibiorum, Mucorcircinelloides f. circinelloides, Mucor circinelloides var.griseocyanus, Mucor flavus, Mucor fuscus, Mucor griseocyanus, Mucorheterosporus, Mucor hiemalis, Mucor hiemalis f. hiemalis, Mucorinaequisporus, Mucor indicus, Mucorjavanicus, Mucor mucedo, Mucormucilagineus, Mucor piriformis, Mucor plasmaticus, Mucor plumbeus, Mucorracemosus, Mucor racemosus f. racemosus, Mucor racemosus f.sphaerosporus, Mucor rouxianus, Mucor rouxii, Mucor sinensis, Mucor sp.,Mucor spinosus, Mucor tuberculisporus, Mucor variisporus, Mucorvariosporus, Mucor wosnessenskii, Phycomyces blakesleeanus, Rhizopusachlamydosporus, Rhizopus arrhizus, Rhizopus chinensis, Rhizopusdelemar, Rhizopus formosaensis, Rhizopusjaponicus, Rhizopus javanicus,Rhizopus microsporus, Rhizopus microsporus var. chinensis, Rhizopusmicrosporus var. oligosporus, Rhizopus microsporus var. rhizopodiformis,Rhizopus nigricans, Rhizopus niveus, Rhizopus oligosporus, Rhizopusoryzae, Rhizopus pygmaeus, Rhizopus rhizopodiformis, Rhizopussemarangensis, Rhizopus sontii, Rhizopus stolonifer, Rhizopus thermosus,Rhizopus tonkinensis, Rhizopus tritici or Rhizopus usamii; Pythiaceaesuch as the genera Phytium; Phytophthora e.g. the species Pythiumdebaryanum, Pythium intermedium, Pythium irregulare, Pythiummegalacanthum, Pythium paroecandrum, Pythium sylvaticum, Pythiumultimum, Phytophthora cactorum, Phytophthora cinnamomi, Phytophthoracitricola, Phytophthora citrophthora, Phytophthora cryptogea,Phytophthora drechsleri, Phytophthora erythroseptica, Phytophthoralateralis, Phytophthora megasperma, Phytophthora nicotianae,Phytophthora nicotianae var. parasitica, Phytophthora palmivora,Phytophthora parasitica or Phytophthora syringae; Sacharomycetaceae suchas the genera Hansenula, Pichia, Saccharomyces, Saccharomycodes,Yarrowia e.g. the species Hansenula anomala, Hansenula californica,Hansenula canadensis, Hansenula capsulata, Hansenula ciferrii, Hansenulaglucozyma, Hansenula henricii, Hansenula holstii, Hansenula minuta,Hansenula nonfermentans, Hansenula philodendri, Hansenula polymorpha,Hansenula saturnus, Hansenula subpelliculosa, Hansenula wickerhamii,Hansenula wingei, Pichia alcoholophila, Pichia angusta, Pichia anomala,Pichia bispora, Pichia burtonii, Pichia canadensis, Pichia capsulata,Pichia carsonii, Pichia cellobiosa, Pichia ciferrii, Pichia farinosa,Pichia fermentans, Pichia finlandica, Pichia glucozyma, Pichiaguilliermondii, Pichia haplophila, Pichia henricii, Pichia holstii,Pichia jadinii, Pichia lindnerii, Pichia membranaefaciens, Pichiamethanolica, Pichia minuta var. minuta, Pichia minuta var.nonfermentans, Pichia norvegensis, Pichia ohmeri, Pichia pastoris,Pichia philodendri, Pichia pini, Pichia polymorpha, Pichia quercuum,Pichia rhodanensis, Pichia sargentensis, Pichia stipitis, Pichiastrasburgensis, Pichia subpelliculosa, Pichia toletana, Pichiatrehalophila, Pichia vini, Pichia xylosa, Saccharomyces aceti,Saccharomyces bailii, Saccharomyces bayanus, Saccharomyces bisporus,Saccharomyces capensis, Saccharomyces carlsbergensis, Saccharomycescerevisiae, Saccharomyces cerevisiae var. ellipsoideus, Saccharomyceschevalieri, Saccharomyces delbrueckii, Saccharomyces diastaticus,Saccharomyces drosophilarum, Saccharomyces elegans, Saccharomycesellipsoideus, Saccharomyces fermentati, Saccharomyces florentinus,Saccharomyces fragilis, Saccharomyces heterogenicus, Saccharomyceshienipiensis, Saccharomyces inusitatus, Saccharomyces italicus,Saccharomyces kluyveri, Saccharomyces krusei, Saccharomyces lactis,Saccharomyces marxianus, Saccharomyces microellipsoides, Saccharomycesmontanus, Saccharomyces norbensis, Saccharomyces oleaceus, Saccharomycesparadoxus, Saccharomyces pastorianus, Saccharomyces pretoriensis,Saccharomyces rosei, Saccharomyces rouxii, Saccharomyces uvarum,Saccharomycodes ludwigii or Yarrowia lipolytica; Saprolegniaceae such asthe genera Saprolegnia e.g. the species Saprolegnia ferax;Schizosacharomycetaceae such as the genera Schizosaccharomyces e.g. thespecies Schizosaccharomyces japonicus var. japonicus,Schizosaccharomyces japonicus var. versatilis, Schizosaccharomycesmalidevorans, Schizosaccharomyces octosporus, Schizosaccharomyces pombevar. malidevorans or Schizosaccharomyces pombe var. pombe; Sodariaceaesuch as the genera Neurospora, Sordaria e.g. the species Neurosporaafricana, Neurospora crassa, Neurospora intermedia, Neurosporasitophila, Neurospora tetrasperma, Sordaria fimicola or Sordariamacrospora; Tuberculariaceae such as the genera Epicoccum, Fusarium,Myrothecium, Sphacelia, Starkeyomyces, Tubercularia e.g. the speciesFusarium acuminatum, Fusarium anthophilum, Fusarium aquaeductuum,Fusarium aquaeductuum var. medium, Fusarium avenaceum, Fusariumbuharicum, Fusarium camptoceras, Fusarium cerealis, Fusariumchlamydosporum, Fusarium ciliatum, Fusarium coccophilum, Fusariumcoeruleum, Fusarium concolor, Fusarium crookwellense, Fusarium culmorum,Fusarium dimerum, Fusarium diversisporum, Fusarium equiseti, Fusariumequiseti var. bullatum, Fusarium eumartii, Fusarium flocciferum,Fusarium fujikuroi, Fusarium graminearum, Fusarium graminum, Fusariumheterosporum, Fusarium incarnatum, Fusarium inflexum, Fusariumjavanicum, Fusarium lateritium, Fusarium lateritium var. majus, Fusariumlongipes, Fusarium melanochlorum, Fusarium merismoides, Fusariummerismoides var. chlamydosporale, Fusarium moniliforme, Fusariummoniliforme var. anthophilum, Fusarium moniliforme var. subglutinans,Fusarium nivale, Fusarium nivale var. majus, Fusarium oxysporum,Fusarium oxysporum f. sp. aechmeae, Fusarium oxysporum f. sp. cepae,Fusarium oxysporum f. sp. conglutinans, Fusarium oxysporum f. sp.cucumerinum, Fusarium oxysporum f. sp. cyclaminis, Fusarium oxysporum f.sp. dianthi, Fusarium oxysporum f. sp. lycopersici, Fusarium oxysporumf. sp. melonis, Fusarium oxysporum f. sp. passiflorae, Fusariumoxysporum f. sp. pisi, Fusarium oxysporum f. sp. tracheiphilum, Fusariumoxysporum f. sp. tuberosi, Fusarium oxysporum f. sp. tulipae, Fusariumoxysporum f. sp. vasinfectum, Fusarium pallidoroseum, Fusarium poae,Fusarium proliferatum, Fusarium proliferatum var. minus, Fusariumredolens, Fusarium redolens f. sp. dianthi, Fusarium reticulatum,Fusarium roseum, Fusarium sacchari var. elongatum, Fusarium sambucinum,Fusarium sambucinum var. coeruleum, Fusarium semitectum, Fusariumsemitectum var. majus, Fusarium solani, Fusarium solani f. sp. pisi,Fusarium sporotrichioides, Fusarium sporotrichioides var. minus,Fusarium sublunatum, Fusarium succisae, Fusarium sulphureum, Fusariurntabacinum, Fusarium tricinctum, Fusarium udum, Fusarium ventricosum,Fusarium verticillioides, Fusarium xylarioides or Fusarium zonatum;Sporobolomycetaceae such as the genera Bullera, Sporobolomyces,Itersonilia e.g. the species Sporobolomyces holsaticus, Sporobolomycesodorus, Sporobolomyces puniceus, Sporobolomyces salmonicolor,Sporobolomyces singularis or Sporobolomyces tsugae; Adelotheciaceae suchas the genera e.g. the species Physcomitrella patens; Dinophyceae suchas the genera Crypthecodinium, Phaeodactylum e.g. the speciesCrypthecodinium cohnli or Phaeodactylum tricomutum; Ditrichaceae such asthe genera Ceratodon, Pleuridium, Astomiopsis, Ditrichum,Philibertiella, Ceratodon, Distichium, Skottsbergia e.g. the speciesCeratodon antarcticus, Ceratodon purpureus, Ceratodon purpureus ssp.convolutes or Ceratodon purpureus ssp. stenocarpus; Prasinophyceae suchas the genera Nephroselmis, Prasinococcus, Scherffelia, Tetraselmis,Mantoniella, Ostreococcus e.g. the species Nephroselmis olivacea,Prasinococcus capsulatus, Scherffelia dubia, Tetraselmis chui,Tetraselmis suecica, Mantoniella squamata or Ostreococcus tauri;Actinomycetaceae such as the genera Actinomyces, Actinobaculum,Arcanobacterium, Mobiluncus e.g. the species Actinomyces bernardiae,Actinomyces bovis, Actinomyces bowdeni, Actinomyces canis, Actinomycescardiffensis, Actinornyces catuli, Actinomyces coleocanis, Actinomycesdenticolens, Actinomyces europaeus, Actinomyces funkei, Actinomycesgeorgiae, Actinomyces gerencseriae, Actinomyces hordeovulneris,Actinomyces howellii, Actinomyces humiferus, Actinomyces hyovaginalis,Actinomyces israeli, Actinomyces marimammalium, Actinomyces meyeri,Actinomyces naeslundii, Actinomyces nasicola, Actinomyces neuii subsp.anitratus, Actinomyces neuii subsp. neuii, Actinomyces odontolyticus,Actinomyces oricola, Actinomyces pyogenes, Actinomyces radicidentis,Actinomyces radingae, Actinomyces slackii, Actinomyces suimastitidis,Actinomyces suis, Actinomyces turicensis, Actinomyces urogenitalis,Actinomyces vaccimaxillae, Actinomyces viscosus, Actinobaculum schaalii,Actinobaculum suis, Actinobaculum urinale, Arcanobacterium bernardiae,Arcanobacterium haemolyticum, Arcanobacterium hippocoleae,Arcanobacterium phocae, Arcanobacterium pluranimalium, Arcanobacteriumpyogenes, Mobiluncus curtisii subsp. curtisii, Mobiluncus curtisiisubsp. holmesii or Mobiluncus mulieris; Bacillaceae such as the generaAmphibacillus, Anoxybacillus, Bacillus, Exiguobacterium,Gracilibacillus, Holobacillus, Saccharococcus, Salibacillus,Virgibacillus e.g. the species Amphibacillus fermentum, Amphibacillustropicus, Amphibacillus xylanus, Anoxybacillus flavithermus,Anoxybacillus gonensis, Anoxybacillus pushchinoensis, Bacillusacidocaldarius, Bacillus acidoterrestris, Bacillus aeolius, Bacillusagaradhaerens, Bacillus agri, Bacillus alcalophilus, Bacillusalginolyticus, Bacillus alvei, Bacillus amyloliquefaciens, Bacillusamylolyticus, Bacillus aneurinilyticus, Bacillus aquimaris, Bacillusarseniciselenatis, Bacillus atrophaeus, Bacillus azotofixans, Bacillusazotoformans, Bacillus badius, Bacillus barbaricus, Bacillusbenzoevorans, Bacillus borstelensis, Bacillus brevis, Bacilluscarboniphilus, Bacillus centrosporus, Bacillus cereus, Bacilluschitinolyticus, Bacillus chondroitinus, Bacillus choshinensis, Bacilluscirculans, Bacillus clarkii, Bacillus clausii, Bacillus coagulans,Bacillus cohnii, Bacillus curdlanolyticus, Bacillus cycloheptanicus,Bacillus decolorationis, Bacillus dipsosauri, Bacillus edaphicus,Bacillus ehimensis, Bacillus endophyticus, Bacillus fastidiosus,Bacillus firmus, Bacillus flexus, Bacillus formosus, Bacillus fumarioli,Bacillus funiculus, Bacillus fusiformis, Bacillus sphaericus subsp.fusiformis, Bacillus galactophilus, Bacillus globisporus, Bacillusglobisporus subsp. marinus, Bacillus glucanolyticus, Bacillus gordonae,Bacillus halmapalus, Bacillus haloalkaliphilus, Bacillushalodenitrificans, Bacillus halodurans, Bacillus halophilus, Bacillushorikoshii, Bacillus horti, Bacillus infernos, Bacillus insolitus,Bacillus jeotgali, Bacillus kaustophilus, Bacillus kobensis, Bacilluskrulwichiae, Bacillus laevolacticus, Bacillus larvae, Bacilluslaterosporus, Bacillus lautus, Bacillus lentimorbus, Bacillus lentus,Bacillus licheniformis, Bacillus luciferensis, Bacillus macerans,Bacillus macquariensis, Bacillus marinus, Bacillus marisflavi, Bacillusmarismortui, Bacillus megaterium, Bacillus methanolicus, Bacillusmigulanus, Bacillus mojavensis, Bacillus mucilaginosus, Bacillusmycoides, Bacillus naganoensis, Bacillus nealsonii, Bacillus neidei,Bacillus niacini, Bacillus okuhidensis, Bacillus oleronius, Bacilluspabuli, Bacillus pallidus, Bacillus pantothenticus, Bacillus parabrevis,Bacillus pasteurii, Bacillus peoriae, Bacillus polymyxa, Bacilluspopilliae, Bacillus pseudalcaliphilus, Bacillus pseudofirmus, Bacilluspseudomycoides, Bacillus psychrodurans, Bacillus psychrophilus, Bacilluspsychrosaccharolyticus, Bacillus psychrotolerans, Bacillus pulvifaciens,Bacillus pumilus, Bacillus pycnus, Bacillus reuszeri, Bacillussalexigens, Bacillus schlegelii, Bacillus selenitireducens, Bacillussilvestris, Bacillus simplex, Bacillus siralis, Bacillus smithii,Bacillus sonorensis, Bacillus sphaericus, Bacillus sporothermodurans,Bacillus stearothermophilus, Bacillus subterraneus, Bacillus subtilissubsp. spizizenii, Bacillus subtilis subsp. subtilis, Bacillusthermantarcticus, Bacillus thermoaerophilus, Bacillus thermoamylovorans,Bacillus thermoantarcticus, Bacillus thermocatenulatus, Bacillusthermocloacae, Bacillus thermodenitrificans, Bacillusthermoglucosidasius, Bacillus thermoleovorans, Bacillus thermoruber,Bacillus thermosphaericus, Bacillus thiaminolyticus, Bacillusthuringiensis, Bacillus tusciae, Bacillus validus, Bacillusvallismorfis, Bacillus vedderi, Bacillus vulcani, Bacillusweihenstephanensis, Exiguobacterium acetylicum, Exiguobacteriumantarcticum, Exiguobacterium aurantiacum, Exiguobacterium undae,Gracilibacillus dipsosauri, Gracilibacillus halotolerans, Halobacillushalophilus, Halobacillus karajensis, Halobacillus litoralis,Halobacillus salinus, Halobacillus trueperi, Saccharococcuscaldoxylosilyticus, Saccharococcus thermophilus, Salibacillusmarismortui, Salibacillus salexigens, Virgibacillus carmonensis,Virgibacillus marismortui, Virgibacillus necropolis, Virgibacilluspantothenticus, Virgibacillus picturae, Virgibacillus proomii orVirgibacillus salexigens, Brevibacteriaceae such as the generaBrevibacterium e.g. the species Brevibacterium acetylicum,Brevibacterium albidum, Brevibacterium ammoniagenes, Brevibacteriumavium, Brevibacterium casei, Brevibacterium citreum, Brevibacteriumdivaricatum, Brevibacterium epidermidis, Brevibacterium fermentans,Brevibacterium frigoritolerans, Brevibacterium halotolerans,Brevibacterium imperiale, Brevibacterium incertum, Brevibacteriumiodinum, Brevibacterium linens, Brevibacterium liquefaciens,Brevibacterium lutescens, Brevibacterium luteum, Brevibacterium lyticum,Brevibacterium mcbrellneri, Brevibacterium otitidis, Brevibacteriumoxydans, Brevibacterium paucivorans, Brevibacterium protophormiae,Brevibacterium pusillum, Brevibacterium saperdae, Brevibacteriumstationis, Brevibacterium testaceum or Brevibacterium vitaeruminis;Corynebacteriaceae such as the genera Corynebacterium e.g. the speciesCorynebacterium accolens, Corynebacterium afermentans subsp.afermentans, Corynebacterium afermentans subsp. lipophilum,Corynebacterium ammoniagenes, Corynebacterium amycolatum,Corynebacterium appendicis, Corynebacterium aquilae, Corynebacteriumargentoratense, Corynebacterium atypicum, Corynebacterium aurimucosum,Corynebacterium auris, Corynebacterium auriscanis, Corynebacteriumbetae, Corynebacterium beticola, Corynebacterium bovis, Coryrnebacteriumcallunae, Corynebacterium camporealensis, Corynebacterium capitovis,Corynebacterium casei, Corynebacterium confusum, Corynebacteriumcoyleae, Corynebacterium cystitidis, Corynebacterium durum,Corynebacterium efficiens, Corynebacterium equi, Corynebacteriumfalsenii, Corynebacterium fascians, Corynebacterium felinum,Corynebacterium flaccumfaciens, Corynebacterium flavescens,Corynebacterium freneyi, Corynebacterium glaucum, Corynebacteriumglucuronolyticum, Corynebacterium glutamicum, Corynebacterium hoagii,Corynebacterium ilicis, Corynebacterium imitans, Corynebacteriuminsidiosum, Corynebacterium iranicum, Corynebacterium jeikeium,Corynebacterium kroppenstedtii, Corynebacterium kutscheri,Corynebacterium lilium, Corynebacterium lipophiloflavum,Corynebacteriurn macginleyi, Corynebacterium mastitidis, Corynebacteriummatruchotii, Corynebacterium michiganense, Corynebacterium michiganensesubsp. tessellarius, Corynebacterium minutissimum, Corynebacteriummooreparkense, Corynebacterium mucifaciens, Corynebacterium mycetoides,Corynebacterium nebraskense, Corynebacterium oortii, Corynebacteriumpaurometabolum, Corynebacterium phocae, Corynebacterium pilosum,Corynebacterium poinsettiae, Corynebacterium propinquum, Corynebacteriumpseudodiphtheriticum, Corynebacterium pseudotuberculosis,Corynebacterium pyogenes, Corynebacterium rathayi, Corynebacteriumrenale, Corynebacterium riegelii, Corynebacterium seminale,Corynebacterium sepedonicum, Corynebacterium simulans, Corynebacteriumsingulare, Corynebacterium sphenisci, Corynebacterium spheniscorum,Corynebacterium striatum, Corynebacterium suicordis, Corynebacteriumsundsvallense, Corynebacterium terpenotabidum, Corynebacteriumtestudinoris, Corynebacterium thomssenii, Corynebacterium tritici,Corynebacterium ulcerans, Corynebacterium urealyticum, Corynebacteriumvariabile, Corynebacterium vitaeruminis or Corynebacterium xerosis;Enterobacteriacae such as the genera Alterococcus, Arsenophonus,Brenneria, Buchnera, Budvicia, Buttiauxella, Calymmatobacterium,Cedecea, Citrobacter, Edwardsiella, Enterobacter, Erwinia, Escherichia,Ewingella, Hafnia, Klebsiella, Kluyvera, Leclercia, Leminorella,Moellerella, Morganella, Obesumbacterium, Pantoea, Pectobacterium,Photorhabdus, Plesiomonas, Pragia, Proteus, Providencia, Rahnella,Saccharobacter, Salmonella, Shigella, Serratia, Sodalis, Tatumella,Trabulsiella, Wigglesworthia, Xenorhabdus, Yersinia and Yokenella e.g.the species Arsenophonus nasoniae, Brenneria alni, Brennerianigrifluens, Brenneria quercina, Brenneria rubrifaciens, Brenneriasalicis, Budvicia aquatica, Buttiauxella agrestis, Buttiauxellabrennerae, Buttiauxella ferragutiae, Buttiauxella gaviniae, Buttiauxellaizardii, Buttiauxella noackiae, Buttiauxella warmboldiae, Cedeceadavisae, Cedecea lapagei, Cedecea neteri, Citrobacter amalonaticus,Citrobacter diversus, Citrobacter freundii, Citrobacter genomospecies,Citrobacter gillenii, Citrobacter intermedium, Citrobacter koseri,Citrobacter murliniae, Citrobacter sp., Edwardsiella hoshinae,Edwardsiella ictaluri, Edwardsiella tarda, Erwinia alni, Erwiniaamylovora, Erwinia ananatis, Erwinia aphidicola, Erwinia billingiae,Erwinia cacticida, Erwinia cancerogena, Erwinia camegieana, Erwiniacarotovora subsp. atroseptica, Erwinia carotovora subsp. betavasculorum, Erwinia carotovora subsp. odorifera, Erwinia carotovora subsp.wasabiae, Erwinia chrysanthemi, Erwinia cypripedii, Erwinia dissolvens,Erwinia herbicola, Erwinia mallotivora, Erwinia milletiae, Erwinianigrifluens, Erwinia nimipressuralis, Erwinia persicina, Erwinia psidii,Erwinia pyrifoliae, Erwinia quercina, Erwinia rhapontici, Erwiniarubrifaciens, Erwinia salicis, Erwinia stewartii, Erwinia tracheiphila,Erwinia uredovora, Escherichia adecarboxylata, Escherichia anindolica,Escherichia aurescens, Escherichia blattae, Escherichia coli,Escherichia coli var. communior, Escherichia coli-mutabile, Escherichiafergusonil, Escherichia hermannii, Escherichia sp., Escherichiavulneris, Ewingella americana, Hafnia alvei, Klebsiella aerogenes,Klebsiella edwardsii subsp. atlantae, Klebsiella ornithinolytica,Klebsiella oxytoca, Klebsiella planticola, Klebsiella pneumoniae,Klebsiella pneumoniae subsp. pneumoniae, Klebsiella sp., Klebsiellaterrigena, Klebsiella trevisanii, Kluyvera ascorbata, Kluyveracitrophila, Kluyvera cochleae, Kluyvera cryocrescens, Kluyverageorgiana, Kluyvera noncitrophila, Kluyvera sp., Leclerciaadecarboxylata, Leminorella grimontii, Leminorella richardii,Moellerella wisconsensis, Morganella morganii, Morganella morganiisubsp. morganii, Morganella morganii subsp. sibonii, Obesumbateriumproteus, Pantoea agglomerans, Pantoea ananatis, Pantoea citrea, Pantoeadispersa, Pantoea punctata, Pantoea stewartii subsp. stewartii, Pantoeaterrea, Pectobacterium atrosepticum, Pectobacterium carotovorum subsp.atrosepticum, Pectobacterium carotovorum subsp. carotovorum,Pectobacterium chrysanthemi, Pectobacterium cypripedii, Photorhabdusasymbiotica, Photorhabdus luminescens, Photorhabdus luminescens subsp.akhurstii, Photorhabdus luminescens subsp. laumondii, Photorhabdusluminescens subsp. luminescens, Photorhabdus sp., Photorhabdustemperata, Plesiomonas shigelloides, Pragia fontium, Proteus hauseri,Proteus ichthyosmius, Proteus inconstans, Proteus mirabilis, Proteusmorganii, Proteus myxofaciens, Proteus penneri, Proteus rettgeri,Proteus shigelloides, Proteus vulgaris, Providencia alcalifaciens,Providencia friedericiana, Providencia heimbachae, Providencia rettgeri,Providencia rustigianii, Providencia stuartii, Rahnella aquatilis,Salmonella abony, Salmonella arizonae, Salmonella bongori, Salmonellacholeraesuis subsp. arizonae, Salmonella choleraesuis subsp. bongori,Salmonella choleraesuis subsp. cholereasuis, Salmonella choleraesuissubsp. diarizonae, Salmonella choleraesuis subsp. houtenae, Salmonellacholeraesuis subsp. indica, Salmonella choleraesuis subsp. salamae,Salmonella daressalaam, Salmonella enterica subsp. houtenae, Salmonellaenterica subsp. salamae, Salmonella enteritidis, Salmonella gallinarum,Salmonella heidelberg, Salmonella panama, Salmonella senftenberg,Salmonella typhimurium, Serratia entomophila, Serratia ficaria, Serratiafonticola, Serratia grimesii, Serratia liquefaciens, Serratiamarcescens, Serratia marcescens subsp. marcescens, Serratia marinorubra,Serratia odorifera, Serratia plymouthensis, Serratia plymuthica,Serratia proteamaculans, Serratia proteamaculans subsp. quinovora,Serratia quinivorans, Serratia rubidaea, Shigella boydii, Shigellaflexneri, Shigella paradysenteriae, Shigella sonnei, Tatumella ptyseos,Xenorhabdus beddingii, Xenorhabdus bovienii, Xenorhabdus luminescens,Xenorhabdus nematophila, Xenorhabdus nematophila subsp. beddingii,Xenorhabdus nematophila subsp. bovienii, Xenorhabdus nematophila subsp.poinarii or Xenorhabdus poinarii; Gordoniaceae such as the generaGordonia, Skermania e.g. the species Gordonia aichiensis, Gordoniaalkanivorans, Gordonia amarae, Gordonia amicalis, Gordonia bronchialis,Gordonia desulfuricans, Gordonia hirsuta, Gordonia hydrophobica,Gordonia namibiensis, Gordonia nitida, Gordonia paraffinivorans,Gordonia polyisoprenivorans, Gordonia rhizosphera, Gordoniarubripertincta, Gordonia sihwensis, Gordonia sinesedis, Gordonia sput,Gordonia terrae or Gordonia wesffafrca; Micrococcaceae such as thegenera Micrococcus, Arthrobacter, Kocuria, Nesterenkonia, Renibacterium,Rothia, Stomatococcus e.g. the species Micrococcus agilis, Micrococcusantarcticus, Micrococcus halobius, Micrococcus kristinae, Micrococcusluteus, Micrococcus lylae, Micrococcus nishinomiyaensis, Micrococcusroseus, Micrococcus sedentarius, Micrococcus varians, Arthrobacteragilis, Arthrobacter albus, Arthrobacter atrocyaneus, Arthrobacteraurescens, Arthrobacter chlorophenolicus, Arthrobacter citreus,Arthrobacter creatinolyticus, Arthrobacter crystallopoietes,Arthrobacter cumminsii, Arthrobacter duodecadis, Arthrobacterflavescens, Arthrobacter flavus, Arthrobacter gandavensis, Arthrobacterglobiformis, Arthrobacter histidinolovorans, Arthrobacter ilicis,Arthrobacter koreensis, Arthrobacter luteolus, Arthrobactermethylotrophus, Arthrobacter mysorens, Arthrobacter nasiphocae,Arthrobacter nicotianae, Arthrobacter nicotinovorans, Arthrobacteroxydans, Arthrobacter pascens, Arthrobacter picolinophilus, Arthrobacterpolychromogenes, Arthrobacter protophormiae, Afthrobacterpsychrolactophilus, Arthrobacter radiotolerans, Arthrobacter ramosus,Arthrobacter rhombi, Arthrobacter roseus, Arthrobacter siderocapsulatus,Arthrobacter simplex, Arthrobacter sulfonivorans, Arthrobactersulfureus, Arthrobacter terregens, Arthrobacter tumescens, Arthrobacteruratoxydans, Arthrobacter ureafaciens, Arthrobacter variabilis,Arthrobacter viscosus, Arthrobacter woluwensis, Kocuria erythromyxa,Kocuria kristinae, Kocuria palustris, Kocuria polaris, Kocuriarhizophila, Kocuria rosea, Kocuria varians, Nesterenkonia halobia,Nesterenkonia lacusekhoensis, Renibacterium salmoninarum, Rothia amarae,Rothia dentocariosa, Rothia mucilaginosa, Rothia nasimurium orStomatococcus mucilaginosus; Mycobacteriaceae such as the generaMycobacterium e.g. the species Mycobacterium africanum, Mycobacteriumagri, Mycobacterium aichiense, Mycobacterium alvei, Mycobacteriumasiaticum, Mycobacterium aurum, Mycobacterium austroafricanum,Mycobacterium bohemicum, Mycobacterium botniense, Mycobacterium brumae,Mycobacterium chelonae subsp. abscessus, Mycobacterium chitae,Mycobacterium chlorophenolicum, Mycobacterium chubuense, Mycobacteriumconfluentis, Mycobacterium cookii, Mycobacterium diernhoferi,Mycobacteriumm doricum, Mycobacterium duvalii, Mycobacterium fallax,Mycobacterium farcinogenes, Mycobacterium flavescens, Mycobacteriumfrederiksbergense, Mycobacterium gadium, Mycobacterium gilvum,Mycobacterium gordonae, Mycobacterium hassiacum, Mycobacterium hibemiae,Mycobacterium hodleri, Mycobacterium holsaticum, Mycobacteriumkomossense, Mycobacterium lacus, Mycobacterium madagascariense,Mycobacterium mageritense, Mycobacterium montefiorense, Mycobacteriummoriokaense, Mycobacterium murale, Mycobacterium neoaurum, Mycobacteriumnonchromogenicum, Mycobacterium obuense, Mycobacterium palustre,Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacteriumphlei, Mycobacterium pinnipedii, Mycobacterium poriferae, Mycobacteriumpulveris, Mycobacterium rhodesiae, Mycobacterium shottsii, Mycobacteriumsphagni, Mycobacterium terrae, Adycobacterium thermoresistibile,Mycobacterium tokaiense, Mycobacterium triviale, Mycobacterium tusciaeor Mycobacterium vanbaalenii; Nocardiaceae such as the genera Nocardia,Rhodococcus e.g. the species Nocardia abscessus, Nocardia africana,Nocardia amarae, Nocardia asteroides, Nocardia autotrophica, Alocardiabeijingensis, Nocardia brasiliensis, Nocardia brevicatena, Nocardiacaishijiensis, Nocardia calcarea, Nocardia carnea, Nocardia cellulans,Nocardia cerradoensis, Nocardia coeliaca, Nocardia corynebacterioides,Nocardia crassostreae, Nocardia cummidelens, Nocardia cyriacigeorgica,Nocardia farcinica, Nocardia flavorosea, Nocardia fluminea, Nocardiagloberula, Nocardia hydrocarbonoxydans, Nocardia ignorata, Nocardiamediterranei, Nocardia nova, Nocardia orientalis, Nocardiaotitidiscaviarum, Nocardia otitidiscaviarum, Nocardia paucivorans,Nocardia petrcleophila, Nocardia pinensis, Nocardia pseudobrasiliensis,Nocardia pseudovaccinii, Nocardia puris, Nocardia restricta, Nocardiarugosa, Nocardia salmonicida, Nocardia saturnea, Nocardia seriolae,Nocardia soli, Nocardia sulphurea, Nocardia transvalersis, Nocardiauniformis, Nocardia vaccinii, Nocardia veterana or Nocardia vinacea;Pseudomonaceae such as the genera Azomonas, Azotobacter, Cellvibrio,Chryseomon as, Flaviomonas, Lampropedia, Mesophilobacter, Morococcus,Oligella, Pseudomonas, Rhizobacter, Rugamonas, Serpens, Thermoleophilum,Xylophilus e.g. the species Azomonas agilis, Azomonas insignis, Azomonasmacrocytogenes, Azotobacter agilis, Azotobacter agilis subsp. armeniae,Azotobacter armeniacus, Azotobacter beijerinckii, Azotobacterchroococcum, Azotobacter indicum, Azotobacter macrocytogenes,Azotobacter miscellum, Azotobacter nigricans subsp. nigricans,Azotobacter paspali, Azotobacter salinestris, Azotobacter sp.,Azotobacter vinelandii, Flavimonas oryzihabitans, Mesophilobactermarinus, Oligella urethralis, Pseudomonas acidovorans, Pseudomonasaeruginosa, Pseudomonas agarici, Pseudomonas alcaligenes, Pseudomonasaminovorans, Pseudomonas amygdali, Pseudomonas andropogonis, Pseudomonasanguilliseptica, Pseudomonas antarctica, Pseudomonas antimicrobica,Pseudomonas antimycetica, Pseudomonas aptata, Pseudomonas arvilla,Pseudomonas asplenii, Pseudomonas atlantica, Pseudomonas atrofaciens,Pseudomonas aureofaciens, Pseudomonas avellanae, Pseudomonas azelaica,Pseudomonas azotocolligans, Pseudomonas balearica, Pseudomonas barkeri,Pseudomonas bathycetes, Pseudomonas beijerinckii, Pseudomonasbrassicacearum, Pseudomonas brenneri, Pseudomonas butanovora,Pseudomonas carboxydoflava, Pseudomonas carboxydohydrogena, Pseudomonascarboxydovorans, Pseudomonas carrageenovora, Pseudomonas caryophylli,Pseudomonas cepacia, Pseudomonas chloritidismutans, Pseudomonaschlororaphis, Pseudomonas cichorii, Pseudomonas citronellolis,Pseudomonas cocovenenans, Pseudomonas compransoris, Pseudomonascongrelans, Pseudomonas coronafaciens, Pseudomonas corrugate,Pseudomonas dacunhae, Pseudomonas delafieldii, Pseudomonas delphinii,Pseudomonas denitrificans, Pseudomonas desmolytica, Pseudomonasdiminuta, Pseudomonas doudoroffii, Pseudomonas echinoides, Pseudomonaselongata, Pseudomonas extorquens, Pseudomonas extremorientalis,Pseudomonas facilis, Pseudomonas ficuserectae, Pseudomonas flava,Pseudomonas flavescens, Pseudomonas fluorescens, Pseudomonas fragi,Pseudomonas frederiksbergensis, Pseudomonas fulgida, Pseudomonasfuscovaginae, Pseudomonas gazotropha, Pseudomonas gladioli, Pseudomonasglathei, Pseudomonas glumae, Pseudomonas graminis, Pseudomonashalophila, Pseudomonas helianthi, Pseudomonas huttiensis, Pseudomonashydrogenothermophila, Pseudommonas hydrogenovora, Pseudomonas indica,Pseudomonas indigofera, Pseudomonas iodinum, Pseudomonas kilonensis,Pseudomonas lachrymans, Pseudomonas lapsa, Pseudomonas lemoignei,Pseudomonas lemonnieri, Pseudomonas lundensis, Pseudomonas luteola,Pseudomonas maltophilia, Pseudomonas marginalis, Pseudomonas marginata,Pseudomonas marina, Pseudomonas meliae, Pseudomonas men docina,Pseudomonas mesophilica, Pseudomonas mixta, Pseudomonas monteilii,Pseudomonas morsprunorum, Pseudomonas multivorans, Pseudomonasnatriegens, Pseudomonas neutica, Pseudomonas nitroreducens, Pseudomonasoleovorans, Pseudomonas oryzihabitans, Pseudomonas ovalis, Pseudomonasoxalaticus, Pseudomonas paeleronii, Pseudomonas paucimobilis,Pseudomonas phaseolicola, Pseudomonas phenazinium, Pseudomonaspickettii, Pseudomonas pisi, Pseudornonas plantari, Pseudomonasplecoglossicida, Pseudomonas poae, Pseudomonas primulae, Pseudomonasproteolytica, Pseudomonas pseudoalcaligenes, Pseudomonaspseudoalcaligenes subsp. konjaci, Pseudomonas pseudoalcaligenes subsp.pseudoalcaligenes, Pseudomonas pseudoflava, Pseudomonas putida,Pseudomonas putida var. naraensis, Pseudomonas putrefaciens, Pseudomonaspyrrocinia, Pseudomonas radiora, Pseudomonas reptilivora, Pseudomonasrhodesiae, Pseudomonas rhodos, Pseudomonas riboflavina, Pseudomonasrubescens, Pseudomonas rubrisubalbicans, Pseudomonas ruhlandii,Pseudomonas saccharophila, Pseudomonas savastanoi, Pseudomonassavastanoi pvar. glycinea, Pseudomonas savastanoi pvar. phaseolicola,Pseudomonas solanacearum, Pseudomonas sp., Pseudomonas spinosa,Pseudomonas stanieri, Pseudomonas stutzeri, Pseudomonas syringae,Pseudomonas syringae pvar. aptata, Pseudomonas syringae pvar.atrofaciens, Pseudomonas syringae pvar. coronafaciens, Pseudomonassyringae pvar. delphinii, Pseudomonas syringae pvar. glycinea,Pseudomonas syringae pvar. helianthi, Pseudomonas syringae pvar.lachrymans, Pseudomonas syringae pvar. lapsa, Pseudomonas syringae pvar.morsprunorum, Pseudomonas syringae pvar. phaseolicola, Pseudomonassyringae pvar. primulae, Pseudomonas syringae pvar. syringae,Pseudomonas syringae pvar. tabaci, Pseudomonas syringae pvar. tomato,Pseudomonas syringae subsp. glycinea, Pseudomonas syringae subsp.savastanoi, Pseudornonas syringae subsp. syringae, Pseudomonas syzygii,Pseudomonas tabaci, Pseudomonas taeniospiralis, Pseudomonastestosteroni, Pseudomonas thermocarboxydovorans, Pseudomonasthermotolerans, Pseudomonas thivervalensis, Pseudomonas tomato,Pseudomonas trivialis, Pseudomonas veronii, Pseudomonas vesicularis,Psetidomonas viridiflava, Pseudomonas viscogena, Pseudomonas woodsii,Rhizobacter dauci, Rhizobacter daucus or Xylophilus ampelinus;Rhizobiaceae such as the genera Agrobacterium, Carbophilus,Chelatobacter, Ensifer, Rhizobium, Sinorhizobium e.g. the speciesAgrobacterium atlanticum, Agrobacterium ferrugineum, Agrobacteriumgelatinovorum, Agrobacterium larrymoorei, Agrobacterium meteori,Agrobacterium radiobacter, Agrobacterium rhizogenes, Agrobacterium rubi,Agrobacteriurn stellulatum, Agrobacterium tumefaciens, Agrobacteriumvitis, Carbophilus carboxidus, Chelatobacter heintzii, Ensiferadhaerens, Ensifer arboris, Ensifer fredii, Ensifer kostiensis, Ensiferkummerowiae, Ensifer medicae, Ensifer meliloti, Ensifer saheli, Ensiferterangae, Ensifer xinjiangensis, Rhizobium ciceri Rhizobium etli,Rhizobium fredii, Rhizobium galegae, Rhizobium gallicum, Rhizobiumgiardinii, Rhizobium hainanense, Rhizobium huakuii, Rhizobiumhuautlense, Rhizobium indigoferae, Rhizobium japonicum, Rhizobiumleguminosarum, Rhizobium loessense, Rhizobium loti, Rhizobium lupini,Rhizobium mediterraneum, Rhizobium meliloti, Rhizobium mongolense,Rhizobium phaseoli, Rhizobium radiobacter, Rhizobium rhizogenes,Rhizobium rubi, Rhizobium sullae, Rhizobium tianshanense, Rhizobiumtrifolii, Rhizobium tropici, Rhizobium undicola, Rhizobium vitis,Sinorhizobium adhaerens, Sinorhizobium arboris, Sinorhizobium fredii,Sinorhizobium kostiense, Sinorhizobium kummerowiae, Sinorhizobiummedicae, Sinorhizobium meliloti, Sinorhizobium morelense, Sinorhizobiumsaheli or Sinorhizobium xinjiangense; Streptomycetaceae such as thegenera Kitasatosprora, Streptomyces, Streptoverticillium e.g. thespecies Streptomyces abikoensis, Streptomyces aburaviensis, Streptomycesachromogenes subsp. achromogenes, Streptomyces achromogenes subsp.rubradiris, Streptomyces acidiscabies, Streptomyces acrimycini,Streptomyces aculeolatus, Streptomyces afghaniensis, Streptomycesalanosinicus, Streptomyces albaduncus, Streptomyces albiaxialis,Streptomyces albidochromogenes, Streptomyces albidoflavus, Streptomycesalbireticuli, Streptomyces albofaciens, Streptomyces alboflavus,Streptomyces albogriseolus, Streptomyces albolongus, Streptomycesalboniger, Streptomyces albospinus, Streptomyces albosporeus subsp.albosporeus, Streptomyces albosporeus subsp. labilomyceticus,Streptomyces alboverticillatus, Streptomyces albovinaceus, Streptomycesalboviridis, Streptomyces albulus, Streptomyces albus subsp. albus,Streptomyces albus subsp. pathocidicus, Streptomyces almquistii,Streptomyces althioticus, Streptomyces amakusaensis, Streptomycesambofaciens, Streptomyces aminophilus, Streptomyces anandii,Streptomyces anthocyanicus, Streptomyces antibioticus, Streptomycesantimycoticus, Streptomyces anulatus, Streptomyces arabicus,Streptomyces ardus, Streptomyces arenae, Streptomyces argenteolus,Streptomyces armeniacus, Streptomyces asiaticus, Streptomycesasterosporus, Streptomyces atratus, Streptomyces atroaurantiacus,Streptomyces atroolivaceus, Streptomyces atrovirens, Streptomycesaurantiacus, Streptomyces aurantiogriseus, Streptomyces aureocirculatus,Streptomyces aureofaciens, Streptomyces aureorectus, Streptomycesaureoversilis, Streptomyces aureoverticillatus, Streptomyces aureus,Streptomyces avellaneus, Streptomyces avermectinius, Streptomycesavermitilis, Streptomyces avidinii, Streptomyces azaticus, Streptomycesazureus, Streptomyces baarnensis, Streptomyces bacillaris, Streptomycesbadius, Streptomyces baldaccii, Streptomyces bambergiensis, Streptomycesbeijiangensis, Streptomyces bellus, Streptomyces bikiniensis,Streptomyces biverticillatus, Streptomyces blastmyceticus, Streptomycesbluensis, Streptomyces bobili, Streptomyces bottropensis, Streptomycesbrasiliensis, Streptomyces bungoensis, Streptomyces cacaoi subsp.asoensis, Streptomyces cacaoi subsp. cacaoi, Streptomyces caelestis,Streptomyces caeruleus, Streptomyces californicus, Streptomyces calvus,Streptomyces canaries, Streptomyces candidus, Streptomyces canescens,Streptomyces cangkringensis, Streptomyces caniferus, Streptomyces canus,Streptomyces capillispiralis, Streptomyces capoamus, Streptomycescarpaticus, Streptomyces carpinensis, Streptomyces catenulae,Streptomyces caviscabies, Streptomyces cavourensis subsp. cavourensis,Streptomyces cavourensis subsp. washingtonensis, Streptomycescellostaticus, Streptomyces celluloflavus, Streptomyces cellulolyticus,Streptomyces cellulosae, Streptomyces champavatii, Streptomyceschartreuses, Streptomyces chattanoogensis, Streptomyces chibaensis,Streptomyces chrestomyceticus, Streptomyces chromofuscus, Streptomyceschryseus, Streptomyces chrysomallus subsp. chrysomallus, Streptomyceschrysomallus subsp. fumigatus, Streptomyces cinereorectus, Streptomycescinereoruber subsp. cinereoruber, Streptomyces cinereoruber subsp.fructofermentans, Streptomyces cinereospinus, Streptomyces cinereus,Streptomyces cinerochromogenes, Streptomyces cinnabarinus, Streptomycescinnamonensis, Streptomyces cinnamoneus, Streptomyces cinnamoneus subsp.albosporus, Streptomyces cinnamoneus subsp. cinnamoneus, Streptomycescinnamoneus subsp. lanosus, Streptomyces cinnamoneus subsp. sparsus,Streptomyces cirratus, Streptomyces ciscaucasicus, Streptomycescitreofluorescens, Streptomyces clavifer, Streptomyces clavuligerus,Streptomyces cochleatus, Streptomyces coelescens, Streptomycescoelicoflavus, Streptomyces coelicolor, Streptomyces coeruleoflavus,Streptomyces coeruleofuscus, Streptomyces coeruleoprunus, Streptomycescoeruleorubidus, Streptomyces coerulescens, Streptomyces collinus,Streptomyces colombiensis, Streptomyces corchorusii, Streptomycescostaricanus, Streptomyces cremeus, Streptomyces crystallinus,Streptomyces curacoi, Streptomyces cuspidosporus, Streptomycescyaneofuscatus, Streptomyces cyaneus, Streptomyces cyanoalbus,Streptomyces cystargineus, Streptomyces daghestanicus, Streptomycesdiastaticus subsp. ardesiacus, Streptomyces diastaticus subsp.diastaticus, Streptomyces diastatochromogenes, Streptomyces distallicus,Streptomyces djakartensis, Streptomyces durhamensis, Streptomycesechinatus, Streptomyces echinoruber, Streptomyces ederensis,Streptomyces ehimensis, Streptomyces endus, Streptomyces enissocaesilis,Streptomyces erumpens, Streptomyces erythraeus, Streptomyceserythrogriseus, Streptomyces eurocidicus, Streptomyces europaeiscabiei,Streptomyces eurythermus, Streptomyces exfoliates, Streptomyces felleus,Streptomyces fervens, Streptomyces fervens subsp. fervens, Streptomycesfervens subsp. melrosporus, Streptomyces filamentosus, Streptomycesfilipinensis, Streptomyces fimbriatus, Streptomyces fimicarius,Streptomyces finlayi, Streptomyces flaveolus, Streptomyces flaveus,Streptomyces flavidofuscus, Streptomyces flavidovirens, Streptomycesflaviscleroticus, Streptomyces flavofungini, Streptomyces flavofuscus,Streptomyces flavogriseus, Streptomyces flavopersicus, Streptomycesflavotricini, Streptomyces flavovariabilis, Streptomyces flavovirens,Streptomyces flavoviridis, Streptomyces flocculus, Streptomycesfloridae, Streptomyces fluorescens, Streptomyces fradiae, Streptomycesfragilis, Streptomyces fulvissimus, Streptomyces fulvorobeus,Streptomyces fumanus, Streptomyces fumigatiscleroticus, Streptomycesgalbus, Streptomyces galilaeus, Streptomyces gancidicus, Streptomycesgardneri, Streptomyces gelaticus, Streptomyces geysiriensis,Streptomyces ghanaensis, Streptomyces gibsonii, Streptomycesglaucescens, Streptomyces glaucosporus, Streptomyces glaucus,Streptomyces globisporus subsp. caucasicus, Streptomyces globisporussubsp. flavofuscus, Streptomyces globisporus subsp. globisporus,Streptomyces globosus, Streptomyces glomeratus, Streptomycesglomeroaurantiacus, Streptomyces gobitricini, Streptomyces goshikiensis,Streptomyces gougerotii, Streptomyces graminearus, Streptomycesgraminofaciens, Streptomyces griseinus, Streptomyces griseoaurantiacus,Streptomyces griseobrunneus, Streptomyces griseocarneus, Streptomycesgriseochromogenes, Streptomyces griseoflavus, Streptomyces griseofuscus,Streptomyces griseoincamatus, Streptomyces griseoloalbus, Streptomycesgriseolosporeus, Streptomyces griseolus, Streptomyces griseoluteus,Streptomyces griseomycini, Streptomyces griseoplanus, Streptomycesgriseorubens, Streptomyces griseoruber, Streptomyces griseorubiginosus,Streptomyces griseosporeus, Streptomyces griseostramineus, Streptomycesgriseoverticillatus, Streptomyces griseoviridis, Streptomyces griseussubsp. alpha, Streptomyces griseus subsp. cretosus, Streptomyces griseussubsp. griseus, Streptomyces griseus subsp. solvifaciens, Streptomyceshachijoensis, Streptomyces halstedii, Streptomyces hawaiiensis,Streptomyces heliomycini, Streptomyces helvaticus, Streptomycesherbaricolor, Streptomyces hiroshimensis, Streptomyces hirsutus,Streptomyces humidus, Streptomyces humiferus, Streptomyces hydrogenans,Streptomyces hygroscopicus subsp. angustmyceticus, Streptomyceshygroscopicus subsp. decoyicus, Streptomyces hygroscopicus subsp.glebosus, Streptomyces hygroscopicus subsp. hygroscopicus, Streptomyceshygroscopicus subsp. ossamyceticus, Streptomyces iakyrus, Streptomycesindiaensis, Streptomyces indigoferus, Streptomyces indonesiensis,Streptomyces intermedius, Streptomyces inusitatus, Streptomycesipomoeae, Streptomyces janthinus, Streptomyces javensis, Streptomyceskanamyceticus, Streptomyces kashmirensis, Streptomyces kasugaensis,Streptomyces katrae, Streptomyces kentuckensis, Streptomyces kifunensis,Streptomyces kishiwadensis, Streptomyces kunmingensis, Streptomyceskurssanovii, Streptomyces labedae, Streptomyces laceyi, Streptomycesladakanum, Streptomyces lanatus, Streptomyces lateritius, Streptomyceslaurentii, Streptomyces lavendofoliae, Streptomyces lavendulae subsp.grasserius, Streptomyces lavendulae subsp. lavendulae, Streptomyceslavenduligriseus, Streptomyces lavendulocolor, Streptomyces levis,Streptomyces libani subsp. libani, Streptomyces libani subsp. rufus,Streptomyces lienomycini, Streptomyces ilacinus, Streptomyces limosus,Streptomyces lincolnensis, Streptomyces lipmanii, Streptomyceslitmocidini, Streptomyces lomondensis, Streptomyces longisporoflavus,Streptomyces longispororuber, Streptomyces longisporus, Streptomyceslongwoodensis, Streptomyces lucensis, Streptomyces luridiscabiei,Streptomyces luridus, Streptomyces lusitanus, Streptomycesluteireticuli, Streptomyces luteogriseus, Streptomyces luteosporeus,Streptomyces luteoverticillatus, Streptomyces lydicus, Streptomycesmacrosporus, Streptomyces malachitofuscus, Streptomyces malachitospinus,Streptomyces malaysiensis, Streptomyces mashuensis, Streptomycesmassasporeus, Streptomyces matensis, Streptomyces mauvecolor,Streptomyces mediocidicus, Streptomyces mediolani, Streptomycesmegasporus, Streptomyces melanogenes, Streptomyces melanosporofaciens,Streptomyces mexicanus, Streptomyces michiganensis, Streptomycesmicroflavus, Streptomyces minutiscleroticus, Streptomyces mirabilis,Streptomyces misakiensis, Streptomyces misionensis, Streptomycesmobaraensis, Streptomyces monomycini, Streptomyces morookaensis,Streptomyces murinus, Streptomyces mutabilis, Streptomyces mutomycini,Streptomyces naganishii, Streptomyces narbonensis, Streptomycesnashvillensis, Streptomyces netropsis, Streptomyces neyagawaensis,Streptomyces niger, Streptomyces nigrescens, Streptomyces nigrifaciens,Streptomyces nitrosporeus, Streptomyces niveiciscabiei, Streptomycesniveoruber, Streptomyces niveus, Streptomyces noboritoensis,Streptomyces nodosus, Streptomyces nogalater, Streptomyces nojiriensis,Streptomyces noursei, Streptomyces novaecaesareae, Streptomycesochraceiscleroticus, Streptomyces odorifer, Streptomycesolivaceiscleroticus, Streptomyces olivaceoviridis, Streptomycesolivaceus, Streptomyces olivochromogenes, Streptomyces olivomycini,Streptomyces olivoreticuli, Streptomyces olivoreticuli subsp.cellulophilus, Streptomyces olivoreticuli subsp. olivoreticuli,Streptomyces olivoverticillatus, Streptomyces olivoviridis, Streptomycesomiyaensis, Streptomyces orinoci, Streptomyces pactum, Streptomycesparacochleatus, Streptomyces paradoxus, Streptomyces parvisporogenes,Streptomyces parvulus, Streptomyces parvus, Streptomyces peucetius,Streptomyces phaeochromogenes, Streptomyces phaeofaciens, Streptomycesphaeopurpureus, Streptomyces phaeoviridis, Streptomyces phosalacineus,Streptomyces pilosus, Streptomyces platensis, Streptomyces plicatus,Streptomyces pluricolorescens, Streptomyces polychromogenes,Streptomyces poonensis, Streptomyces praecox, Streptomycesprasinopilosus, Streptomyces prasinosporus, Streptomyces prasinus,Streptomyces prunicolor, Streptomyces psammoticus, Streptomycespseudoechinosporeus, Streptomyces pseudogriseolus, Streptomycespseudovenezuelae, Streptomyces pulveraceus, Streptomyces puniceus,Streptomyces puniciscabiei, Streptomyces purpeofuscus, Streptomycespurpurascens, Streptomyces purpureus, Streptomycespurpurogeneiscleroticus, Streptomyces racemochromogenes, Streptomycesrameus, Streptomyces ramulosus, Streptomyces rangoonensis, Streptomycesrecifensis, Streptomyces rectiverticillatus, Streptomycesrectiviolaceus, Streptomyces regensis, Streptomyces resistomycificus,Streptomyces reticuliscabiei, Streptomyces rhizosphaericus, Streptomycesrimosus subsp. paromomycinus, Streptomyces rimosus subsp. rimosus,Streptomyces rishiriensis, Streptomyces rochei, Streptomycesroseiscleroticus, Streptomyces roseodiastaticus, Streptomycesroseoflavus, Streptomyces roseofulvus, Streptomyces roseolilacinus,Streptomyces roseolus, Streptomyces roseosporus, Streptomycesroseoverticillatus, Streptomyces roseoviolaceus, Streptomycesroseoviridis, Streptomyces rubber, Streptomyces rubiginosohelvolus,Streptomyces rubiginosus, Streptomyces rubrogriseus, Streptomycesrutgersensis subsp. castelarensis, Streptomyces rutgersensis subsp.rutgersensis, Streptomyces salmonis, Streptomyces sampsonii,Streptomyces sanglieri, Streptomyces sannanensis, Streptomycessapporonensis, Streptomyces scabiei, Streptomyces sclerotialus,Streptomyces scopiformis, Streptomyces seoulensis, Streptomycesseptatus, Streptomyces setae, Streptomyces setonii, Streptomycesshowdoensis, Streptomyces sindenensis, Streptomyces sioyaensis,Streptomyces somaliensis, Streptomyces sparsogenes, Streptomycesspectabilis, Streptomyces speibonae, Streptomyces speleomycini,Streptomyces spheroids, Streptomyces spinoverrucosus, Streptomycesspiralis, Streptomyces spiroverticillatus, Streptomyces spitsbergensis,Streptomyces sporocinereus, Streptomyces sporoclivatus, Streptomycesspororaveus, Streptomyces sporoverrucosus, Streptomyces stelliscabiei,Streptomyces stramineus, Streptomyces subrutilus, Streptomycessulfonofaciens, Streptomyces sulphurous, Streptomyces syringium,Streptomyces tanashiensis, Streptomyces tauricus, Streptomyces tendae,Streptomyces termitum, Streptomyces thermoalcalitolerans, Streptomycesthermoautotrophicus, Streptomyces thermocarboxydovorans, Streptomycesthermocarboxydus, Streptomyces thermocoprophilus, Streptomycesthermodiastaticus, Streptomyces thermogriseus, Streptomycesthermolineatus, Streptomyces thermonitrificans, Streptomycesthermospinosisporus, Streptomyces thermoviolaceus subsp. apingens,Streptomyces thermoviolaceus subsp. thermoviolaceus, Streptomycesthermovulgaris, Streptomyces thioluteus, Streptomyces torulosus,Streptomyces toxytricini, Streptomyces tricolor, Streptomycestubercidicus, Streptomyces tuirus, Streptomyces turgidiscabies,Streptomyces umbrinus, Streptomyces variabilis, Streptomyces variegates,Streptomyces varsoviensis, Streptomyces vastus, Streptomyces venezuelae,Streptomyces vinaceus, Streptomyces vinaceusdrappus, Streptomycesviolaceochromogenes, Streptomyces violaceolatus, Streptomycesviolaceorectus, Streptomyces violaceoruber, Streptomycesviolaceorubidus, Streptomyces violaceus, Streptomyces violaceusniger,Streptomyces violarus, Streptomyces violascens, Streptomyces violatus,Streptomyces violens, Streptomyces virens, Streptomyces virginiae,Streptomyces viridiflavus, Streptomyces viridiviolaceus, Streptomycesviridobrunneus, Streptomyces viridochromogenes, Streptomycesviridodiastaticus, Streptomyces viridosporus, Streptomycesvitaminophileus, Streptomyces vitaminophilus, Streptomyces wedmorensis,Streptomyces werraensis, Streptomyces willmorei, Streptomycesxanthochromogenes, Streptomyces xanthocidicus, Streptomycesxantholiticus, Streptomyces xanthophaeus, Streptomyces yatensis,Streptomyces yerevanensis, Streptomyces yogyakartensis, Streptomycesyokosukanensis, Streptomyces yunnanensis, Streptomyces zaomyceticus,Streptoverticillium abikoense, Streptoverticillium albireticuli,Streptoverticillium alboverticillatum, Streptoverticillium album,Streptoverticillium ardum, Streptoverticillium aureoversale,Streptoverticillium aureoversile, Streptoverticillium baldaccii,Streptoverticillium biverticillatum, Streptoverticillium blastmyceticum,Streptoverticillium cinnamoneum subsp. albosporum, Streptomycescinnamoneus subsp. albosporus, Streptoverticillium cinnamoneum subsp.cinnamoneum, Streptoverticillium cinnamoneum subsp. lanosum,Streptoverticillium cinnamoneum subsp. sparsum, Streptoverticilliumdistallicum, Streptoverticillium ehimense, Streptoverticilliumeurocidicum, Streptoverticillium fervens subsp. fervens,Streptoverticillium fervens subsp. melrosporus, Streptoverticilliumflavopersiclim, Streptoverticillium griseocarneum, Streptoverticilliumgriseoverticillatum, Streptoverticillium hachijoense,Streptoverticillium hiroshimense, Streptoverticillium kashmirense,Streptoverticillium kentuckense, Streptoverticillium kishiwadense,Streptoverticillium ladakanum, Streptoverticillium lavenduligriseum,Streptoverticillium lilacinum, Streptoverticillium luteoverticillatum,Streptoverticillium mashuense, Streptoverticillium mobaraense,Streptoverticillium morookaense, Streptoverticillium netropsis,Streptoverticillium olivomycini, Streptomyces olivomycini,Streptoverticillium olivoreticuli subsp. cellulophilum,Streptoverticillium olivoreticuli subsp. olivoreticuli,Streptoverticillium olivoreticulum, Streptoverticillium olivoreticulumsubsp. cellulophilum, Streptoverticillium olivoverticillatum,Streptoverticillium orinoci, Streptoverticillium parvisporogenes,Streptoverticillium parvisporogenum, Streptoverticilliumrectiverticillatum, Streptoverticillium reticulum subsp. protomycicum,Streptoverticillium roseoverticillatum, Streptoverticillium salmonis,Streptoverticillium sapporonense, Streptoverticillium septatum,Streptoverticillium syringium, Streptoverticillium thioluteum,Streptoverticillium verticillium subsp. quantum, Streptoverticilliumverticillium subsp. tsukushiense or Streptoverticillium viridoflavum.

Particular preferred strains are strains selected from the groupconsisting of Bacillaceae, Brevibacteriaceae, Corynebacteriaceae,Nocardiaceae, Mycobacteriaceae, Streptomycetaceae, Enterobacteriaceaesuch as Bacillus circulans, Bacillus subtilis, Bacillus sp.,Brevibacterium albidum, Brevibacterium album, Brevibacterium cerinum,Brevibacterium flavum, Brevibacterium glutamigenes, Brevibacteriumiodinum, Brevibacterium ketoglutamicum, Brevibacterium lactofermentum,Brevibacterium linens, Brevibacterium roseum, Brevibacteriumsaccharolyticum, Brevibacterium sp., Corynebacterium acetoacidophilum,Corynebacterium acetoglutamicum, Corynebacterium ammoniagenes,Corynebacterium glutamicum (=Micrococcus glutamicum), Corynebacteriummelassecola, Corynebacterium sp., Nocardia rhodochrous (Rhodococcusrhodochrous), Mycobacterium rhodochrous, Streptomyces lividans andEscherichia coli especially Escherichia coli K12.

In addition particular preferred strains are strains selected from thegroup consisting of Cryptococcaceae, Saccharomycetaceae,Schizosaccharomycetacease such as the genera Candida, Hansenula, Pichia,Saccharomyces and Schizosaccharomyces preferred are strains selectedfrom the group consisting of the species Rhodotorula rubra, Rhodotorulaglutinis, Rhodotorula graminis, Yarrowia lipolytica, Sporobolomycessalmonicolor, Sporobolomyces shibatanus, Saccharomyces cerevisiae,Candida boidinii, Candida bombicola, Candida cylindracea, Candidaparapsilosis, Candida rugosa, Candida tropicalis, Pichia methanolica andPichia pastoris. All abovementioned host organisms are also useable assource organisms for the nucleic acid sequences of the invention.Cryptococcaceae, Saccharomycetaceae, Schizosaccharomycetacease such asthe genera Candida, Hansenula, Pichia, Saccharomyces andSchizosaccharomyces are preferred source organisms.

In the event that the transgenic host organism is a non-human animalsuch as Caenorhaditis elegans or a plant, plant tissue or plant cellsuch as plants selected from the group consisting of the familiesAnacardiaceae, Asteraceae, Apiaceae, Betulaceae, Boraginaceae,Brassicaceae, Bromeliaceae, Caricaceae, Cannabaceae, Convolvulaceae,Chenopodiaceae, Cucurbitaceae, Elaeagnaceae, Ericaceae, Euphorbiaceae,Fabaceae, Geraniaceae, Gramineae, Juglandaceae, Lauraceae, Leguminosae,Linaceae or perennial grass, fodder crops, vegetables, ornamentals andArabidopsis thaliana. Such plants are either grown on a solid medium oras cells in a liquid medium, which is known to the skilled worker andsuits the organism. Furthermore such plants can be grown in soil ortherelike. After the growing phase, the organisms can be harvested. Theplants or plant cells or the recovered, and if desired isolated, aminoacids can then be processed further directly into foodstuffs or animalfeeds or for other applications, for example according to thedisclosures made in EP-B-0 533 039 or EP-A-0 615 693, which areexpressly incorporated herein by reference. The harvested material canbe purified in the customary manner by extraction and precipitation orvia ion exchangers and other methods known to the person skilled in theart and described herein below. Products of these different workupprocedures are plants or plant material containing the desired aminoacids or amino acid compositions which still comprise parts of theplants and/or cell components in different amounts, advantageously inthe range of from 0 to 99% by weight, preferably below 80% by weight,especially preferably between below 50% by weight.

Anacardiaceae such as the genera Pistacia, Mangifera, Anacardium e.g.the species Pistacia vera [pistachios, Pistazie], Mangifer indica[Mango] or Anacardium occidentale [Cashew]; Asteraceae such as thegenera Calendula, Carthamus, Centaurea, Cichorium, Cynara, Helianthus,Lactuca, Locusta, Tagetes, Valeriana e.g. the species Calendulaofficinalis [Marigold], Carthamus tinctorius [safflower], Centaureacyanus [cornflower], Cichorium intybus [blue daisy], Cynara scolymus[Artichoke], Helianthus annus [sunflower], Lactuca sativa, Lactucacrispa, Lactuca esculenta, Lactuca scariola L. ssp. sativa, Lactucascariola L. var. integrata, Lactuca scariola L. var. integrifolia,Lactuca sativa subsp. romana, Locusta communis, Valeriana locusta[lettuce], Tagetes lucida, Tagetes erecta or Tagetes tenuifolia[Marigold]; Apiaceae such as the genera Daucus e.g. the species Daucuscarota [carrot]; Betulaceae such as the genera Corylus e.g. the speciesCorylus avellana or Corylus colurna [hazelnut]; Boraginaceae such as thegenera Borago e.g. the species Borago officinalis [borage]; Brassicaceaesuch as the genera Brassica, Melanosinapis, Sinapis, Arabidopsis e.g.the species Brassica napus, Brassica rapa ssp. [canola, oilseed rape,turnip rape], Sinapis arvensis Brassicajuncea, Brassicajuncea var.juncea, Brassica juncea var. crispifolia, Brassica juncea var. foliosa,Brassica nigra, Brassica sinapioides, Melanosinapis communis [mustard],Brassica oleracea [fodder beet] or Arabidopsis thaliana; Bromeliaceaesuch as the genera Anana, Bromelia e.g. the species Anana comosus,Ananas ananas or Bromelia comosa [pineapple]; Caricaceae such as thegenera Carica e.g. the species Carica papaya [papaya]; Cannabaceae suchas the genera Cannabis e.g. the species Cannabis sative [hemp],Convolvulaceae such as the genera Ipomea, Convolvulus e.g. the speciesIpomoea batatus, Ipomoea pandurata, Convolvulus batatas, Convolvulustiliaceus, Ipomoea fastigiata, Ipomoea tiliacea, Ipomoea triloba orConvolvulus panduratus [sweet potato, Man of the Earth, wild potato],Chenopodiaceae such as the genera Beta, i.e. the species Beta vulgaris,Beta vulgaris var. altissima, Beta vulgaris var. Vulgaris, Betamaritima, Beta vulgaris var. perennis, Beta vulgaris var. conditiva orBeta vulgaris var. esculenta [sugar beet]; Cucurbitaceae such as thegenera Cucubita e.g. the species Cucurbita maxima, Cucurbita mixta,Cucurbita pepo or Cucurbita moschata [pumpkin, squash]; Elaeagnaceaesuch as the genera Elaeagnus e.g. the species Olea europaea [olive];Ericaceae such as the genera Kalmia e.g. the species Kalmia latifolia,Kalmia angustifolia, Kalmia microphylla, Kalmia polifolia, Kalmiaoccidentalis, Cistus chamaerhodendros or Kalmia lucida [American laurel,broad-leafed laurel, calico bush, spoon wood, sheep laurel, alpinelaurel, bog laurel, western bog-laurel, swamp-laurel]; Euphorbiaceaesuch as the genera Manihot, Janipha, Jatropha, Ricinus e.g. the speciesManihot utilissima, Janipha manihot, Jatropha manihot, Manihot aipil,Manihot dulcis, Manihot manihot, Manihot melanobasis, Manihot esculenta[manihot, arrowroot, tapioca, cassaya] or Ricinus communis [castor bean,Castor Oil Bush, Castor Oil Plant, Palma Christi, Wonder Tree]; Fabaceaesuch as the genera Pisum, Albizia, Cathormion, Feuillea, Inga,Pithecolobium, Acacia, Mimosa, Medicajo, Glycine, Dolichos, Phaseolus,Soja e.g. the species Pisum sativum, Pisum arvense, Pisum humile [pea],Albizia berteriana, Albizia julibrissin, Albizia lebbeck, Acaciaberteriana, Acacia littoralis, Albizia berteriana, Albizzia berteriana,Cathormion berteriana, Feuillea berteriana, Inga fragrans,Pithecellobium berterianum, Pithecellobium fragrans, Pithecolobiumberterianum, Pseudalbizzia berteriana, Acacia julibrissin, Acacia nemu,Albizia nemu, Feuilleea julibrissin, Mimosa julibrissin, Mimosaspeciosa, Sericanrda julibrissin, Acacia lebbeck, Acacia macrophylla,Albizia lebbek, Feuilleea lebbeck, Mimosa lebbeck, Mimosa speciosa[bastard logwood, silk tree, East Indian Walnut], Medicago sativa,Medicago falcata, Medicago varia [alfalfa] Glycine max Dolichos soja,Glycine gracilis, Glycine hispida, Phaseolus max, Soja hispida or Sojamax [soybean]; Geraniaceae such as the genera Pelargonium, Cocos, Oleume.g. the species Cocos nucifera, Pelargonium grossularioides or Oleumcocois [coconut]; Gramineae such as the genera Saccharum e.g. thespecies Saccharum officinarum; Juglandaceae such as the genera Juglans,Wallia e.g. the species Juglans regia, Juglans ailanthifolia, Juglanssieboldiana, Juglans cinerea, Wallia cinerea, Juglans bixbyi, Juglanscalifornica, Juglans hindsii, Juglans intermedia, Juglansjamaicensis,Juglans major, Juglans microcarpa, Juglans nigra or Wallia nigra[walnut, black walnut, common walnut, persian walnut, white walnut,butternut, black walnut]; Lauraceae such as the genera Persea, Lauruse.g. the species laurel Laurus nobilis [bay, laurel, bay laurel, sweetbay], Persea americana Persea americana, Persea gratissima or Perseapersea [avocado]; Leguminosae such as the genera Arachis e.g. thespecies Arachis hypogaea [peanut]; Linaceae such as the genera Linum,Adenolinum e.g. the species Linum usitatissimum, Linum humile, Linumaustriacum, Linum bienne, Linum angustifolium, Linum catharticum, Linumflavum, Linum grandiflorum, Adenolinum grandiflorum, Linum lewisii,Linum narbonense, Linum perenne, Linum perenne var. lewisii, Linumpratense or Linum trigynum [flax, linseed]; Lythrarieae such as thegenera Punica e.g. the species Punica granatum [pomegranate]; Malvaceaesuch as the genera Gossypium e.g. the species Gossypium hirsutum,Gossypium arboreum, Gossypium barbadense, Gossypium herbaceum orGossypium thurberi [cotton]; Musaceae such as the genera Musa e.g. thespecies Musa nana, Musa acuminata, Musa paradisiaca, Musa spp. [banana];Onagraceae such as the genera Camissonia, Oenothera e.g. the speciesOenothera biennis or Camissonia brevipes [primrose, evening primrose];Palmae such as the genera Elacis e.g. the species Elaeis guineensis [oilplam]; Papaveraceae such as the genera Papaver e.g. the species Papaverorientale, Papaver rhoeas, Papaver dubium [poppy, oriental poppy, cornpoppy, field poppy, shirley poppies, field poppy, long-headed poppy,long-pod poppy]; Pedaliaceae such as the genera Sesamum e.g. the speciesSesamum indicum [sesame]; Piperaceae such as the genera Piper, Artanthe,Peperomia, Steffensia e.g. the species Piper aduncum, Piper amalago,Piper angustifolium, Piper auritum, Piper betel, Piper cubeba, Piperlongum, Piper nigrum, Piper retrofractum, Artanthe adunca, Artantheelongata, Peperomia elongata, Piper elongatum, Steffensia elongata.[Cayenne pepper, wild pepper]; Poaceae such as the genera Hordeum,Secale, Avena, Sorghum, Andropogon, Holcus, Panicum, Oryza, Zea,Triticum e.g. the species Hordeum vulgare, Hordeum jubatum, Hordeummurinum, Hordeum secalinum, Hordeum distichon Hordeum aegiceras, Hordeumhexastichon., Hordeum hexastichum, Hordeum irregulare, Hordeum sativum,Hordeum secalinum [barley, pearl barley, foxtail barley, wall barley,meadow barley], Secale cereale [rye], Avena sativa, Avena fatua, Avenabyzantina, Avena fatua var. sativa, Avena hybrida [oat], Sorghumbicolor, Sorghum halepense, Sorghum saccharatum, Sorghum vulgare,Andropogon drummondi, Holcus bicolor, Holcus sorghum, Sorghumaethiopicum, Sorghum arundinaceum, Sorghum caffrorum, Sorghum cernuum,Sorghum dochna, Sorghum drummondi, Sorghum durra, Sorghum guineense,Sorghum lanceolatum, Sorghum nervosum, Sorghum saccharatum, Sorghumsubglabrescens, Sorghum verticilliflorum, Sorghum vulgare, Holcushalepensis, Sorghum miliaceum millet, Panicum militaceum [Sorghum,millet], Oryza sativa, Oryza latifolia [rice], Zea mays [corn, maize]Triticum aestivum, Triticum durum, Triticum turgidum, Triticum hybernum,Triticum macha, Triticum sativum or Triticum vulgare [wheat, breadwheat, common wheat], Proteaceae such as the genera Macadamia e.g. thespecies Macadamia intergrifolia [macadamia]; Rubiaceae such as thegenera Coffea e.g. the species Cofea spp., Coffea arabica, Coffeacanephora or Coffea liberica [coffee]; Scrophulariaceae such as thegenera Verbascum e.g. the species Verbascum blattaria, Verbascumchaixii, Verbascum densiflorum, Verbascum lagurus, Verbascumlongifolium, Verbascum lychnitis, Verbascum nigrum, Verbascum olympicum,Verbascum phlomoides, Verbascum phoenicum, Verbascum pulverulentum orVerbascum thapsus [mullein, white moth mullein, nettle-leaved mullein,dense-flowered mullein, silver mullein, long-leaved mullein, whitemullein, dark mullein, greek mullein, orange mullein, purple mullein,hoary mullein, great mullein]; Solanaceae such as the genera Capsicum,Nicotiana, Solanum, Lycopersicon e.g. the species Capsicum annuum,Capsicum annuum var. glabriusculum, Capsicum frutescens [pepper],Capsicum annuum [paprika], Nicotiana tabacum, Nicotiana alata, Nicotianaattenuate, Nicotiana glauca, Nicotiana langsdorffi, Nicotianaobtusifolia, Nicotiana quadrivalvis, Nicotiana repanda, Nicotianarustica, Nicotiana sylvestris [tobacco], Solanum tuberosum [potato],Solanum melongena [egg-plant] (Lycopersicon esculentum, Lycopersiconlycopersicum., Lycopersicon pyriforme, Solanum integrifolium or Solanumlycopersicum [tomato]; Sterculiaceae such as the genera Theobroma e.g.the species Theobroma cacao [cacao]; Theaceae such as the generaCamellia e.g. the species Camellia sinensis) [tea]. All abovementionedhost organisms are also useable as source organisms for the nucleic acidsequences of the invention.

Particular preferred plants are plants selected from the groupconsisting of maize, soja, canola, wheat, barley, triticale, rice,linseed, sunflower, potato and Arabidopsis.

With regard to the nucleic acid sequence as depicted in SEQ ID NO: 1,SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11,SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO:23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ IDNO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82,SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO:92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ IDNO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ IDNO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128,SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ IDNO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151,SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ IDNO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169,SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ IDNO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187,SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ IDNO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205,SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ IDNO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ IDNO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253,SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ IDNO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271,SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ IDNO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298,SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ IDNO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ IDNO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334,SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ IDNO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ IDNO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370,SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ IDNO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388,SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ IDNO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406,SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ IDNO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424,SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ IDNO: 434 or SEQ ID NO: 440 a nucleic acid construct which contains saidnucleic acid sequence or an organism (=transgenic organism) which istransformed with said nucleic acid sequence or said nucleic acidconstruct, “transgene” means all those constructs which have beenbrought about by genetic manipulation methods and in which either

-   a) the nucleic acid sequence as depicted in SEQ ID NO: 1, SEQ ID NO:    3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID    NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23,    SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID    NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72,    SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID    NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90,    SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID    NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO:    108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116,    SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ    ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID    NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO:    , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155,    SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ    ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID    NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO:    181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189,    SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ    ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID    NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO:    215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,    SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ    ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID    NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO:    261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269,    SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ    ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID    NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO:    304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,    SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ    ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID    NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO:    338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346,    SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ    ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID    NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO:    372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380,    SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ    ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID    NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:    406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414,    SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ    ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID    NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or a derivative thereof,    or-   b) a genetic regulatory element, for example a promoter, which is    functionally linked to the nucleic acid sequence as depicted in SEQ    ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9,    SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID    NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29,    SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID    NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,    SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID    NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96,    SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ    ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID    NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO:    122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130,    SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ    ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO:    153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161,    SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ    ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID    NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:    187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195,    SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ    ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID    NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO:    228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236,    SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ    ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID    NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO:    267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284,    SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ    ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID    NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO:    310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318,    SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ    ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID    NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO:    344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,    SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ    ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID    NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO:    378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386,    SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ    ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID    NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO:    412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,    SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ    ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or a    derivative thereof, or-   c) (a) and (b)    is/are not present in its/their natural genetic environment or    has/have been modified by means of genetic manipulation methods, it    being possible for the modification to be, by way of example, a    substitution, addition, deletion, inversion or insertion of one or    more nucleotide radicals. “Natural genetic environment” means the    natural chromosomal locus in the organism of origin or the presence    in a genomic library. In the case of a genomic library, the natural,    genetic environment of the nucleic acid sequence is preferably at    least partially still preserved. The environment flanks the nucleic    acid sequence at least on one side and has a sequence length of at    least 50 bp, preferably at least 500 bp, particularly preferably at    least 1000 bp, very particularly preferably at least 5000 bp.

However, transgenic also means that the nucleic acids according to theinvention are located at their natural position in the genome of anorganism, but that the sequence has been modified in comparison with thenatural sequence and/or that the regulatory sequences of the naturalsequences have been modified. Preferably, transgenic/recombinant is tobe understood as meaning the expression of the nucleic acids used in theprocess according to the invention in a non-natural position in thegenome, that is to say the expression of the nucleic acids is homologousor, preferably, heterologous. This expression can be transiently or of asequence integrated stably into the genome.

The use of the nucleic acid sequence according to the invention or ofthe nucleic acid construct according to the invention for the generationof transgenic plants is therefore also subject matter of the invention.

The fine chemical, which is synthesized in the organism, in particularthe microorganism, the plant or animal cell, the plant or animal tissueor the plant, of the invention can be isolated if desired. Depending onthe use of the fine chemical, different purities resulting from thepurification may be advantageous as will be described herein below.

In an advantageous embodiment of the invention, the organism takes theform of a plant whose amino acid content is modified advantageouslyowing to the modified activity of a nucleic acid molecule of the presentinvention expressed. This is important for plant breeders since, forexample, the nutritional value of plants for monogastric animals islimited by a few essential amino acids such as lysine, threonine ormethionine. After the biological activity of the protein of theinvention has been reduced, decreased or deleted, or after the expression of nucleic acid molecule or polypeptide according to the inventionhas been reduced or decreased, the transgenic plant generated thus isgrown on or in a nutrient medium or else in the soil and subsequentlyharvested. The plants or parts thereof, e.g. the leaves, roots, flowers,and/or stems and/or other harvestable material as described below, canthen be used directly as foodstuffs or animal feeds or else be furtherprocessed. Again, the amino acids can be purified further in thecustomary manner via extraction and precipitation or via ion exchangersand other methods known to the person skilled in the art and describedherein below. Products which are suitable for various applications andwhich result from these different processing procedures are amino acidsor amino acid compositions which can still comprise further plantcomponents in different amounts, advantageously in the range of from 0to 99% by weight, preferably from below 90% by weight, especiallypreferably below 80% by weight. The plants can also advantageously beused directly without further processing, e.g. as feed or forextraction.

Another embodiment of the invention is a process for the modification ofthe nucleic acid molecules of the invention encoded by the host organismfor example by random mutagenesis with chemicals, radiation or UV-lightor side directed mutagenesis in such a manner that the production of thefine chemical is increased. This embodiment of the invention shall bedeemed as transgenic in the sense of the invention.

The process described above may also produce chemically pure amino acidsor amino acid compositions. To this end, the amino acids or the aminoacid compositions are isolated in the known manner from an organismaccording to the invention, such as the microorganisms, non-human animalor the plants, and/or their culture medium in which or on which theorganisms had been grown. These chemically pure amino acids or aminoacid compositions are advantageous for applications in the field of thefood industry, the cosmetics industry or the pharmaceutical industry.

Thus, the content of plant components and preferably also furtherimpurities is as low as possible, and the abovementioned amino acids areobtained in as pure form as possible. In these applications, the contentof plant components advantageously amounts to less than 10%, preferably1%, more preferably 0.1%, very especially preferably 0.01% or less.

Accordingly, the fine chemical produced by the present invention is atleast 0.1% by weight pure, preferably more than 1% by weight pure, morepreferred 10% by weight pure, even more preferred are more than 50, 60,70 or 80% by weight purity, even more preferred are more than 90weight-% purity, most preferred are 95% by weight, 99% by weight ormore.

In this context, the amount of the fine chemical in a cell of theinvention may be increased according to the process of the invention byat least a factor of 1.1, preferably at least a factor of 1.5; 2; or 5,especially preferably by at least a factor of 10 or 30, very especiallypreferably by at least a factor of 50, in comparison with the wild type,control or reference. Preferrably, said increase is found a tissue, morepreferred in an organism or in a harvestable part thereof.

In principle, the amino acids produced can be increased in two ways bythe process according to the invention. The pool of free amino acids, inparticular of methionine, and/or the content of protein-bound methioninemay advantageously be increased. It may be advantageous to increase thepool of free amino acids in the transgenic organisms by the processaccording to the invention in order to isolate pure methionine. Galiliet al., Transgenic Res. 2000 showed, that enhancing the synthesis ofthreonine by a feed back insensitive aspartate kinase leads not only toin increase in free threonine but also in protein bound threonine. Inanother preferred embodiment of the invention is a combination of thereduction, decrease or deletion of the nucleic acid sequence or theprotein of the invention together with the transformation of a proteinor polypeptide, which functions as a sink for the desired amino acid forexample methionine, lysine or threonine in the organism is useful toincrease the production of the fine chemical (see U.S. Pat. No.5,589,616, WO 96/38574, WO 97/07665, WO 97/28247, U.S. Pat. Nos.4,886,878, 5,082,993 and 5,670,635).

In a preferred embodiment, methionine and/or threonine are produced inaccordance with the invention and, if desired, are isolated. Theproduction of further amino acids such as lysine and of amino acidmixtures by the process according to the invention is advantageous.

In the case of the fermentation of microorganisms, the above-mentionedamino acids may accumulate in the medium and/or the cells. Ifmicroorganisms are used in the process according to the invention, thefermentation broth can be processed after the cultivation. Depending onthe requirement, all or some of the biomass can be removed from thefermentation broth by separation methods such as, for example,centrifugation, filtration, ultrafiltration, decanting or a combinationof these methods, or else the biomass can be left in the fermentationbroth. The fermentation broth can subsequently be reduced, orconcentrated, with the aid of known methods such as, for example, rotaryevaporator, thin-layer evaporator, falling film evaporator, by reverseosmosis or by nanofiltration. This concentrated fermentation broth cansubsequently be processed by lyophilization, spray drying, spraygranulation or by other methods.

To purify an amino acid, a product-containing fermentation broth fromwhich the biomass has been separated may be subjected to chromatographywith a suitable resin such as ion exchange resin for example anion orcation exchange resin, hydrophobic resin or hydrophilic resin forexample epoxy resin, polyurethane resin or polyacrylamide resin, orresin for separation according to the molecular weight of the compoundsfor example polyvinyl chloride homopolymer resin or resins composed forexample of polymers of acrylic acid, crosslinked with polyalkenyl ethersor divinyl glycol such as Carbopol®, Pemulen® and Noveon®, where all orsome of the desired product or of the impurities are retained on thechromatography resin. If necessary these chromatography steps may berepeated using the same or other chromatography resins. The skilledworker is familiar with the choice of suitable chromatography resins andtheir most effective use. The purified product may be concentrated byfiltration or ultrafiltration and stored at a temperature, which ensuresthe maximum stability of the product. Preferably amino acids arepurified by using ion exchange chromatographies known by the personskilled in the art.

The identity and purity of the compound(s) isolated can be determined byprior-art techniques. They encompass high-performance liquidchromatography (HPLC), spectroscopic methods, mass spectrometry (MS),staining methods, thin-layer chromatography, NIRS, enzyme assays ormicrobiological assays. These analytical methods are compiled in: Pateket al. [(1994) Appl. Environ. Microbiol. 60:133-140]; Malakhova et al.[(1996) Biotekhnologiya 11 27-32]; and Schmidt et al. [(1998) BioprocessEngineer. 19:67-70. Ulmann's Encyclopedia of Industrial Chemistry (1996)Bd. A27, VCH Weinheim, pp. 89-90, pp. 521-540, pp. 540-547, pp. 559-566,575-581 and pp. 581-587;] Michal, G [(1999) Biochemical Pathways: AnAtlas of Biochemistry and Molecular Biology, John Wiley and Sons];Fallon, A. et al. [(1987) Applications of HPLC in Biochemistry in:Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17].

In the inventive process as mentioned above preferably the reduction,decrease or deletion of the biological activity represented by proteinof the invention is achieved by reducing, decreasing or deleting theexpression of at least one nucleic acid molecule, wherein the nucleicacid molecule is selected from the group consisting of:

-   a) nucleic acid molecule encoding, preferably at least the mature    form, of the polypeptide shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID    NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,    SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID    NO: 26, SEQ ID NO: 23, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,    SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID    NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83,    SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID    NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101,    SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ    ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID    NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:    127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,    SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ    ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID    NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:    168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,    SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ    ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID    NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:    202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,    SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ    ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO:    235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243,    SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ    ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID    NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO:    283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291,    SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ    ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID    NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO:    317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325,    SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ    ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID    NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:    351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,    SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ    ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID    NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO:    385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393,    SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ    ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID    NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO:    419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427,    SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or    SEQ ID NO: 441;-   b) nucleic acid molecule comprising, preferably at least the mature    form, of the nucleic acid molecule shown in SEQ ID NO: 1, SEQ ID NO:    3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID    NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23,    SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID    NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72,    SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID    NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90,    SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID    NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO:    108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116,    SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ    ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID    NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO:    , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155,    SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ    ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID    NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO:    181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189,    SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ    ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID    NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO:    215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,    SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ    ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID    NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO:    261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269,    SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ    ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID    NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO:    304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,    SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ    ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID    NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO:    338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346,    SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ    ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID    NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO:    372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380,    SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ    ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID    NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:    406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414,    SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ    ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID    NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440;-   c) nucleic acid molecule comprising a nucleic acid sequence, which,    as a result of the degeneracy of the genetic code, can be derived    from a polypeptide sequence depicted in SEQ ID NO: 2, SEQ ID NO: 4,    SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:    14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ    ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:    65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ    ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO:    83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ    ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO:    101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,    SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ    ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID    NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO:    135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143,    SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ    ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID    NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO:    176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,    SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ    ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID    NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO:    210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,    SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID    NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:    243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256,    SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ    ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID    NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:    291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,    SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ    ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID    NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:    325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333,    SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ    ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID    NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:    359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367,    SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ    ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID    NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:    393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401,    SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ    ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID    NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO:    427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435    or SEQ ID NO: 441 and having the biological activity represented by    protein as depicted in SEQ ID NO: 2, SEQ ID NO: 8, SEQ ID NO: 20,    SEQ ID NO: 65, SEQ ID NO: 152, SEQ ID NO: 229 or SEQ ID NO: 283;-   d) nucleic acid molecule encoding a polypeptide having at least 50%    identity with the amino acid sequence of the polypeptide encoded by    the nucleic acid molecule of (a) to (c) and having the biological    activity represented by protein as depicted in SEQ ID NO: 2, SEQ ID    NO: 8, SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO: 152, SEQ ID NO: 229    or SEQ ID NO: 283;-   e) nucleic acid molecule which comprises a polynucleotide which is    obtained by amplifying a cDNA library or a genomic library using the    primers depicted in SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ    ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 144, SEQ ID NO:    145, SEQ ID NO: 221, SEQ ID NO: 222, SEQ ID NO: 244, SEQ ID NO: 245,    SEQ ID NO: 436 or SEQ ID NO: 437 and having the biological activity    represented by protein as depicted in SEQ ID NO: 2, SEQ ID NO: 8,    SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO: 152, SEQ ID NO: 229 or SEQ    ID NO: 283;-   f) nucleic acid molecule which encodes a polypeptide, the    polypeptide being derived by substituting, deleting and/or adding    one or more amino acids of the amino acid sequence of the    polypeptide encoded by the nucleic acid molecules (a) to (d),    preferably to (a) to (b) or (c) and encoding a polypeptide having    the biological activity represented by protein as depicted in SEQ ID    NO: 2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO: 152,    SEQ ID NO: 229 or SEQ ID NO: 283;-   g) nucleic acid molecule which encodes a fragment or an epitope of a    polypeptide which is encoded by one of the nucleic acid molecules    of (a) to (e), preferably to (a) to (b) or (c) and encoding a    polypeptide having the biological activity represented by protein as    depicted in SEQ ID NO: 2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO:    65, SEQ ID NO: 152, SEQ ID NO: 229 or SEQ ID NO: 283;-   h) nucleic acid molecule encoding a polypeptide which is isolated    with the aid of monoclonal or polyclonal antibodies against a    polypeptide encoded by one of the nucleic acid molecules of (a) to    (e), preferably to (a) to (b) or (c), and having the biological    activity represented by the protein as depicted in SEQ ID NO: 2, SEQ    ID NO: 8, SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO: 152, SEQ ID NO:    229 or SEQ ID NO: 283;-   i) nucleic acid molecule encoding a polypeptide comprising the    consensus sequence shown in SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO:    146, SEQ ID NO: 147, SEQ ID NO: 148, SEQ ID NO: 149, SEQ ID NO: 150,    SEQ ID NO: 223, SEQ ID NO: 224, SEQ ID NO: 225, SEQ ID NO: 226, SEQ    ID NO: 227, SEQ ID NO: 246, SEQ ID NO: 247, SEQ ID NO: 248, SEQ ID    NO: 273, SEQ ID NO: 274, SEQ ID NO: 275, SEQ ID NO: 276, SEQ ID NO:    277, SEQ ID NO: 278, SEQ ID NO: 279, SEQ ID NO: 280, SEQ ID NO: 281,    SEQ ID NO: 438, SEQ ID NO: 439 or SEQ ID NO: 442 and having the    biological activity represented by the protein as depicted in SEQ ID    NO: 2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO: 152,    SEQ ID NO: 229 or SEQ ID NO: 283;-   j) nucleic acid molecule encoding a polypeptide having the    biological activity represented by the protein SEQ ID NO: 2, SEQ ID    NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ    ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO:    24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ    ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO:    73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ    ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO:    91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ    ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID    NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO:    117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125,    SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ    ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID    NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO:    158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,    SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ    ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID    NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO:    192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,    SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ    ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID    NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID    NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO:    241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254,    SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ    ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID    NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO:    289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297,    SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ    ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID    NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:    323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331,    SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ    ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID    NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:    357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365,    SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ    ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID    NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO:    391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399,    SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ    ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID    NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO:    425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433,    SEQ ID NO: 435 or SEQ ID NO: 441; and-   k) nucleic acid molecule which is obtainable by screening a suitable    nucleic acid library under stringent hybridisation conditions with a    probe comprising one of the sequences of the nucleic acid molecule    of (a) or (b) or with a fragment thereof having at least 15 nt,    preferably 20 nt, 30 nt, 50 nt, 100 nt, 200 nt or 500 nt of the    nucleic acid molecule characterized in (a) to (c) and encoding a    polypeptide having the biological activity represented by protein as    depicted in SEQ ID NO: 2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO:    65, SEQ ID NO: 152, SEQ ID NO: 229 or SEQ ID NO: 283    -   or which comprises a sequence which is complementary thereto.

In one embodiment, the nucleic acid molecule used in the processdistinguishes over the sequence depicted in SEQ ID NO: 1, SEQ ID NO: 3,SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13,SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO:25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ IDNO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84,SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO:94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ IDNO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112,SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ IDNO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130,SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ IDNO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153,SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ IDNO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171,SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ IDNO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189,SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ IDNO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207,SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ IDNO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232,SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ IDNO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255,SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ IDNO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282,SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ IDNO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300,SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ IDNO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318,SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ IDNO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336,SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ IDNO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354,SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ IDNO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372,SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ IDNO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390,SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ IDNO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408,SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ IDNO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426,SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ IDNO: 440 by at least one or more nucleotides or does not consist of thesequence shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO:7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO:19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ IDNO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO:88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ IDNO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106,SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ IDNO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124,SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ IDNO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: ,SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ IDNO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165,SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ IDNO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ IDNO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201,SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ IDNO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ IDNO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249,SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ IDNO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267,SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ IDNO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ IDNO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ IDNO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330,SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ IDNO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348,SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ IDNO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366,SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ IDNO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ IDNO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402,SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ IDNO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ IDNO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440. In oneembodiment, the nucleic acid molecule of the present invention is lessthan 100%, 99.999%, 99.99%, 99.9% or 99% identical to the sequence shownin SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9,SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO:21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ IDNO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80,SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO:90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ IDNO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108,SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ IDNO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126,SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ IDNO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ IDNO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167,SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ IDNO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185,SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ IDNO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203,SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ IDNO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228,SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ IDNO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251,SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ IDNO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269,SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ IDNO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296,SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ IDNO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314,SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ IDNO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332,SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ IDNO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350,SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ IDNO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368,SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ IDNO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386,SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ IDNO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404,SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ IDNO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422,SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ IDNO: 432, SEQ ID NO: 434 or SEQ ID NO: 440. In another embodiment, thenucleic acid molecule does not consist of the sequence shown in SEQ IDNO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ IDNO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31;SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO:72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ IDNO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100,SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ IDNO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118,SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ IDNO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136,SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO:169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO:205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO:230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO:253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO:271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO:316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO:334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO:352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO:370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO:388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO:424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQID NO: 434 or SEQ ID NO: 440.

Unless otherwise specified, the terms “polynucleotides”, “nucleic acid”and “nucleic acid molecule” are interchangeably in the present context.Unless otherwise specified, the terms “peptide”, “polypeptide” and“protein” are interchangeably in the present context. The term“sequence” may relate to polynucleotides, nucleic acids, nucleic acidmolecules, peptides, polypeptides and proteins, depending on the contextin which the term “sequence” is used. The terms “gene(s)”,“polynucleotide”, “nucleic acid sequence”, “nucleotide sequence”, or“nucleic acid molecule(s)” as used herein refers to a polymeric form ofnucleotides of any length, either ribonucleotides ordeoxyribonucleotides. The terms refer only to the primary structure ofthe molecule.

Thus, the terms “gene(s)”, “polynucleotide”, “nucleic acid sequence”,“nucleotide sequence”, or “nucleic acid molecule(s)” as used hereininclude double- and single-stranded DNA and RNA. They also include knowntypes of modifications, for example, methylation, “caps”, substitutionsof one or more of the naturally occurring nucleotides with an analog.Preferably, the DNA or RNA sequence of the invention comprises a codingsequence encoding the herein defined polypeptide.

A “coding sequence” is a nucleotide sequence, which is transcribed intomRNA and/or translated into a polypeptide when placed under the controlof appropriate regulatory sequences. The boundaries of the codingsequence are determined by a translation start codon at the 5′-terminusand a translation stop codon at the 3′-terminus. A coding sequence caninclude, but is not limited to mRNA, cDNA, recombinant nucleotidesequences or genomic DNA, while introns may be present as well undercertain circumstances.

Nucleic acid molecules with the sequence shown in SEQ ID NO: 1, SEQ IDNO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ IDNO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64,SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO:74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ IDNO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102,SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ IDNO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120,SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ IDNO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138,SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO:153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO:171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO:189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO:207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO:232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO:255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO:282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO:300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO:318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO:336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO:354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO:372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO:390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO:408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO:426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 orSEQ ID NO: 440 nucleic acid molecules which are derived from the aminoacid sequences shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ IDNO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ IDNO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ IDNO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115,SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ IDNO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ IDNO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ IDNO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ IDNO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ IDNO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ IDNO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ IDNO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ IDNO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ IDNO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305,SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ IDNO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ IDNO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ IDNO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ IDNO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ IDNO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ IDNO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ IDNO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 or their derivatives orhomologues encoding polypeptides with the enzymatic or biologicalactivity of protein of the invention or conferring the fine chemicalincrease after reducing, decreasing or deleting its expression oractivity in the process according to the invention. These sequences arecloned in such a manner into nucleic acid constructs that their activityis reduced, decreased or deleted, either individually or in combinationwith other sequences involved in the biosynthesis of amino acids. Thesenucleic acid constructs enable an optimal synthesis of the amino acidsproduced in the process according to the invention.

Nucleic acid molecules, which are advantageous for the process accordingto the invention and which encode polypeptides with the biologicalactivity represented by the protein of the invention and/or conferringthe fine chemical increase can be determined from generally accessibledatabases. Those, which must be mentioned, in particular in this contextare general gene databases such as the EMBL database (Stoesser G. etal., Nucleic Acids Res 2001, Vol. 29, 17-21), the GenBank database(Benson D. A. et al., Nucleic Acids Res 2000, Vol. 28, 15-18), or thePIR database (Barker W. C. et al., Nucleic Acids Res. 1999, Vol. 27,39-43). It is furthermore possible to use organism-specific genedatabases for determining advantageous sequences, in the case of yeastfor example advantageously the SGD database (Cherry J. M. et al.,Nucleic Acids Res. 1998, Vol. 26, 73-80) or the MIPS database (Mewes H.W. et al., Nucleic Acids Res. 1999, Vol. 27, 44-48), in the case of E.coli the GenProtEC database (http://web.bham.ac.uk/bcm4ght6/res.html),and in the case of Arabidopsis the TAIR-database (Huala, E. et al.,Nucleic Acids Res. 2001 Vol. 29(1), 102-5) or the MIPS database.

The nucleic acid molecules used in the process according to theinvention take the form of isolated nucleic acid sequences, which encodepolypeptides with the biological activity of the protein of theinvention enabling the fine chemical increase by reducing, decreasing ordeleting their activity. The nucleic acid sequence(s) used in theprocess for the production of the fine chemical in transgenic organismsoriginate advantageously from an eukaryote but may also originate from aprokaryote or an archebacterium, thus it can derived from e.g. amicroorganism, an animal or a plant.

For the purposes of the invention, as a rule the plural is intended toencompass the singular and vice versa.

In order to improve the introduction of the nucleic acid sequences andthe reduction, decrease or deletion of the sequences in the transgenicorganisms, which are used in the process, the nucleic acid sequences areincorporated into a nucleic acid construct and/or a vector in such amanner that they are reduced, decreased or deleted in respect to thebiological activity either on the nucleic acid sequence expression levelor on the level of the polypeptide or protein encoded by said sequences.In addition to the herein described sequences which are used in theprocess according to the invention, further nucleic acid sequences,advantageously of biosynthesis genes of the amino acid produced in theprocess according to the invention, may additionally be present in thenucleic acid construct or in the vector and may be introduced into theorganism together. However, these additional sequences may also beintroduced into the organisms via other, separate nucleic acidconstructs or vectors.

Using the herein mentioned cloning vectors and transformation methodssuch as those which are published and cited in: Plant Molecular Biologyand Biotechnology (CRC Press, Boca Raton, Fla.), chapter 6/7, pp. 71-119(1993); F. F. White, Vectors for Gene Transfer in Higher Plants; in:Transgenic Plants, vol. 1, Engineering and Utilization, Ed.: Kung and R.Wu, Academic Press, 1993, 15-38; B. Jenes et al., Techniques for GeneTransfer, in: Transgenic Plants, vol. 1, Engineering and Utilization,Ed.: Kung and R. Wu, Academic Press (1993), 128-143; Potrykus, Annu.Rev. Plant Physiol. Plant Molec. Biol. 42 (1991), 205-225)) and furthercited below, the nucleic acids may be used for the recombinantmodification of a wide range of organisms, in particular prokaryotic oreukaryotic microorganisms or plants, so that they become a better andmore efficient producer of the amino acids produced in the processaccording to the invention. This improved production, or productionefficiency, of the amino acids or products derived there from, such asmodified proteins, can be brought about by a direct effect of themanipulation or by an indirect effect of this manipulation.

In one embodiment, the nucleic acid molecule according to the inventionoriginates from a plant, such as a plant selected from the familiesAceraceae, Anacardiaceae, Apiaceae, Asteraceae, Brassicaceae, Cactaceae,Cucurbitaceae, Euphorbiaceae, Fabaceae, Malvaceae, Nymphaeaceae,Papaveraceae, Rosaceae, Salicaceae, Solanaceae, Arecaceae, Bromeliaceae,Cyperaceae, lridaceae, Liliaceae, Orchidaceae, Gentianaceae, Labiaceae,Magnoliaceae, Ranunculaceae, Carifolaceae, Rubiaceae, Scrophulariaceae,Caryophyllaceae, Ericaceae, Polygonaceae, Violaceae, Juncaceae orPoaceae and preferably from a plant selected from the group of thefamilies Apiaceae, Asteraceae, Brassicaceae, Cucurbitaceae, Fabaceae,Papaveraceae, Rosaceae, Solanaceae, Liliaceae or Poaceae. Preferred arecrop plants and in particular plants mentioned herein above as hostplants such as the families and genera mentioned above for examplepreferred the species Anacardium occidentale, Calendula officinalis,Carthamus tinctorius, Cichoriurn intybus, Cynara scolymus, Helianthusannus, Tagetes lucida, Tagetes erecta, Tagetes tenuifolia; Daucuscarota; Corylus avellana, Corylus colurna, Borago officinalis; Brassicanapus, Brassica rapa ssp., Sinapis arvensis, Brassica juncea, Brassicajuncea var. juncea, Brassica juncea var. crispifolia, Brassica junceavar. foliosa, Brassica nigra, Brassica sinapioides, Melanosinapiscommunis, Brassica oleracea, Arabidopsis thaliana, Anana comosus, Ananasananas, Bromelia comosa, Carica papaya, Cannabis sative, Ipomoeabatatus, Ipomoea pandurata, Convolvulus batatas, Convolvulus tiliaceus,Ipomoea fastigiata, Ipomoea tiliacea, Ipomoea triloba, Convolvuluspanduratus, Beta vulgaris, Beta vulgaris var. altissima, Beta vulgarisvar. vulgaris, Beta maritima, Beta vulgaris var. perennis, Beta vulgarisvar. conditiva, Beta vulgaris var. esculenta, Cucurbita maxima,Cucurbita mixta, Cucurbita pepo, Cucurbita moschate, Olea europaea,Manihot utilissima, Janipha manihot, Jatropha manihot, Manihot aipil,Manihot dulcis, Manihot manihot, Manihot melanobasis, Manihot esculenta,Ricinus communis, Pisum sativum, Pisum arvense, Pisum humile, Medicagosativa, Medicago falcata, Medicago varia, Glycine max, Dolichos soja,Glycine gracilis, Glycine hispida, Phaseolus max, Soja hispida, Sojamax, Cocos nucifera, Pelargonium grossularioides, Oleum cocoas, Laurusnobilis, Persea americana, Arachis hypogaea, Linum usitatissimum, Linumhumile, Linum austriacum, Linum bienne, Linum angustifolium, Limmncatharticum, Linum flavum, Linum grandiflorum, Adenolinum grandiflorum,Linum lewisii, Linum narbonense, Linum perenne, Linum perenne var.lewisii, Linum pretense, Linum trigynum, Punica granatum, Gossypiumhirsutum, Gossypium arboreum, Gossypium barbadense, Gossypium herbaceum,Gossypium thurberi, Musa nana, Musa acuminata, Musa paradisiacae Musaspp., Elaeis guineensis, Papaver orientale, Papaver rhoeas, Papaverdubium, Sesamum indicum, Piper aduncum, Piper amalago, Piperangustifolium, Piper auritum, Piper betel, Piper cubeba, Piper longum,Piper nigrum, Piper retrofractum, Artanthe adunca, Artanthe elongata,Peperomia elongata, Piper elongatum, Steffensia elongata, Hordeumvulgare, Hordeumfubatum, Hordeum murinum, Hordeum secalinum, Hordeumdistichon Hordeum aegiceras, Hordeum hexastichon, Hordeum hexastichum,Hordeum irregulare, Hordeum sativum, Hordeum secalinum, Avena sativa,Avena fatua, Avena byzantina, Avena fatua var. sativa, Avena hybrida,Sorghum bicolor, Sorghum halepense, Sorghum saccharatum, Sorghumvulgare, Andropogon drummondi, Holcus bicolor, Holcus sorghum, Sorghumaethiopicum, Sorghum arundinaceum, Sorghum caffrorum, Sorghurn cernuum,Sorghum dochna, Sorghum drummondii, Sorghum durra, Sorghum guineense,Sorghum lanceolatum, Sorghum nervosum, Sorghum saccharatum, Sorghumsubglabrescens, Sorghum verticilliflorum, Sorghum vulgare, Holcushalepensis, Sorghum miliaceum millet, Panicum militaceum, Zea mays,Triticum aestivum, Triticum durum, Triticum turgidum, Triticum hybemum,Triticum macha, Triticum sativum or Triticum vulgare, Cofea spp., Coffeaarabica, Coffea canephora, Coffea liberica, Capsicum annuum, Capsicumannuum var. glabriusculum, Capsicum frutescens, Capsicum annuum,Nicotiana tabacum, Solanum tuberosum, Solarium melongena, Lycopersiconesculentum, Lycopersicon lycopersicum, Lycopersicon pyriforme, Solanumintegrifolium, Solanum lycopersicum, Theobroma cacao or Camelliasinensis.

In one embodiment, the nucleic acid molecule sequence originatesadvantageously from a microorganism as mentioned above under hostorganism such as a fungus for example the genera Aspergillus,Penicillium or Claviceps or from yeasts such as the genera Pichia,Torulopsis, Hansenula, Schizosaccharomyces, Candida, Rhodotorula orSaccharomyces, very especially advantageously from the yeast of thefamily Saccharomycetaceae, such as the advantageous genus Saccharomycesand the very advantageous genus and species Saccharomyces cerevisiae forthe production of the fine chemical in microorganisms. The skilledworker knows other suitable sources for the production of finechemicals, which present also useful nucleic acid molecule sources. Theyinclude in general all prokaryotic or eukaryotic cells, preferablyunicellular microorganisms, such as fungi like the genus Claviceps orAspergillus or gram-positive bacteria such as the genera Bacillus,Corynebacterium, Micrococcus, Brevibacterium, Rhodococcus, Nocardia,Caseobacter or Arthrobacter or gram-negative bacteria such as the generaEscherichia, Flavobacterium or Salmonella, or yeasts such as the generaRhodotorula, Hansenula or Candida.

Production strains which are especially advantageously selected in theprocess according to the invention are microorganisms selected from thegroup of the families Actinomycetaceae, Bacillaceae, Brevibacteriaceae,Corynebacteriaceae, Enterobacteriacae, Gordoniaceae, Micrococcaceae,Mycobacteriaceae, Nocardiaceae, Pseudomonaceae, Rhizobiaceae,Streptomycetaceae, Chaetomiaceae, Choanephoraceae, Cryptococcaceae,Cunninghamellaceae, Demetiaceae, Moniliaceae, Mortierellaceae,Mucoraceae, Pythiaceae, Sacharomycetaceae, Saprolegniaceae,Schizosacharomycetaceae, Sodariaceae, Sporobolomycetaceae,Tuberculariaceae, Adelotheciaceae, Dinophyceae, Ditrichaceae andPrasinophyceaeor of the genera and species consisting of Hansenulaanomala, Candida utilis, Claviceps purpurea, Bacillus circulans,Bacillus subtilis, Bacillus sp., Brevibacterium albidum, Brevibacteriumalbum, Brevibacterium cerinum, Brevibacterium flavum, Brevibacteriumglutamigenes, Brevibacterium iodinum, Brevibacterium ketoglutamicum,Brevibacterium Jactofermentum, Brevibacterium linens, Brevibacteriumroseum, Brevibacterium saccharolyticum, Brevibacterium sp.,Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum,Corynebacterium ammoniagenes, Corynebacterium glutamicum (=Micrococcusglutamicum), Corynebacterium melassecola, Corynebacterium sp. orEscherichia coli, specifically Escherichia coli K12 and its describedstrains. All abovementioned organisms can in princible also function ashost organisms.

However, it is also possible to use artificial sequences, which differpreferably in one or more bases from the nucleic acid sequences found inorganisms, or in one or more amino acid molecules from polypeptidesequences found in organisms, and which mediate a polypeptide havingabove-mentioned activity, e.g. having the biological activity of theprotein of the invention or conferring the fine chemical increase afterreducing, decreasing or deleting its expression or activity.

In the process according to the invention nucleic acid sequences can beused, which if appropriate, contain synthetic, non-natural or modifiednucleotide bases, which can be incorporated into DNA or RNA. Saidsynthetic, non-natural or modified bases can for example increase thestability of the nucleic acid molecule outside or inside a cell. Thenucleic acid molecules of the invention can contain the samemodifications as aforementioned.

As used in the present context the term “nucleic acid molecule” may alsoencompass the untranslated sequence located at the 3′ and at the 5′ endof the coding gene region, for example at least 500, preferably 200,especially preferably 100, nucleotides of the sequence upstream of the5′ end of the coding region and at least 100, preferably 50, especiallypreferably 20, nucleotides of the sequence downstream of the 3′ end ofthe coding gene region. It is often advantageous only to choose thecoding region for cloning and expression purposes. In the event forexample the RNAi or antisense technology is used also the 5′- and/or3′-regions can advantageously be used.

Preferably, the nucleic acid molecule used in the process according tothe invention or the nucleic acid molecule of the invention is anisolated nucleic acid molecule.

An “isolated” polynucleotide or nucleic acid molecule is separated fromother polynucleotides or nucleic acid molecules, which are present inthe natural source of the nucleic acid molecule. An isolated nucleicacid molecule may be a chromosomal fragment of several kb, orpreferably, a molecule only comprising the coding region of the gene.Accordingly, an isolated nucleic acid molecule of the invention maycomprise chromosomal regions, which are adjacent 5′ and 3′ or furtheradjacent chromosomal regions, but preferably comprises no such sequenceswhich naturally flank the nucleic acid molecule sequence in the genomicor chromosomal context in the organism from which the nucleic acidmolecule originates (for example sequences which are adjacent to theregions encoding the 5′- and 3′-UTRs of the nucleic acid molecule). Invarious embodiments, the isolated nucleic acid molecule used in theprocess according to the invention may, for example comprise less thanapproximately 5 kb, 4 kb, 3 kb, 2 kb, 1 kb, 0.5 kb or 0.1 kb nucleotidesequences which naturally flank the nucleic acid molecule in the genomicDNA of the cell from which the nucleic acid molecule originates.

The nucleic acid molecules used in the process, for example a nucleicacid molecule with a nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 3,SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13,SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO:25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ IDNO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84,SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO:94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ IDNO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112,SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ IDNO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130,SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ IDNO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153,SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ IDNO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171,SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ IDNO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189,SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ IDNO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207,SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ IDNO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232,SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ IDNO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255,SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ IDNO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282,SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ IDNO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300,SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ IDNO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318,SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ IDNO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336,SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ IDNO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354,SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ IDNO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372,SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ IDNO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390,SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ IDNO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408,SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ IDNO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426,SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ IDNO: 440 or of a part thereof can be isolated using molecular-biologicalstandard techniques and the sequence information provided herein. Also,for example a homologous sequence or homologous, conserved sequenceregions at the DNA or amino acid level can be identified with the aid ofcomparison algorithms. The former can be used as hybridization probesunder standard hybridization techniques (for example those described inSambrook et al., Molecular Cloning: A Laboratory Manual. 2nd Ed., ColdSpring Harbor Laboratory, Cold Spring Harbor Laboratory Press, ColdSpring Harbor, N.Y., 1989) for isolating further nucleic acid sequencesuseful in this process. A nucleic acid molecule encompassing a completesequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19,SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO:29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ IDNO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88,SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO:98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO:116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO:134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ IDNO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157,SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ IDNO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175,SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ IDNO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193,SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ IDNO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211,SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ IDNO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236,SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ IDNO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259,SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ IDNO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286,SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ IDNO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304,SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ IDNO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322,SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ IDNO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340,SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ IDNO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358,SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ IDNO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376,SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ IDNO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394,SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ IDNO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412,SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ IDNO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430,SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or a part thereof mayadditionally be isolated by polymerase chain reaction, oligonucleotideprimers based on this sequence or on parts thereof being used. Forexample, a nucleic acid molecule comprising the complete sequence orpart thereof can be isolated by polymerase chain reaction usingoligonucleotide primers which have been generated on the basis of thisvery sequence. For example, mRNA can be isolated from cells [for exampleby means of the guanidinium thiocyanate extraction method of Chirgwin etal. (1979) Biochemistry 18:5294-5299] and cDNA can be generated by meansof reverse transcriptase (for example Moloney MLV reverse transcriptase,available from Gibco/BRL, Bethesda, Md., or AMV reverse transcriptase,obtainable from Seikagaku America, Inc., St.Petersburg, Fla.). Syntheticoligonucleotide primers for the amplification by means of polymerasechain reaction can be generated on the basis of a sequence shown herein,for example the sequence shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO:5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO:15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ IDNO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76,SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO:86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ IDNO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104,SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ IDNO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122,SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ IDNO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140,SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO:155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO:173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO:191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO:209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO:234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO:257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO:284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO:302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO:320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO:338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO:356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO:374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO:392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO:410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO:428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 orthe sequences derived from SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16,SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO:28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ IDNO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87,SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO:97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO:133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO:158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO:176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO:194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO:212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441. Such primers canbe used to amplify nucleic acids sequences for example from cDNAlibraries or from genomic libraries and identify nucleic acid molecules,which are useful in the inventive process and which have the biologicalactivity represented by the protein as depicted in SEQ ID NO: 2, SEQ IDNO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ IDNO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO:75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ IDNO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103,SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ IDNO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ IDNO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139,SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ IDNO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ IDNO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182,SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ IDNO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ IDNO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO:235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO:258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO:285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO:303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO:321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO:339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO:375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO:411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO:429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441.Advantageously the primers as depicted in SEQ ID NO: 58, SEQ ID NO: 59,SEQ ID NO: 60, SEQ ID NO: 61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO:144, SEQ ID NO: 145, SEQ ID NO: 221, SEQ ID NO: 222, SEQ ID NO: 244, SEQID NO: 245, SEQ ID NO: 436 or SEQ ID NO: 437 are used (see Table 1).

TABLE 1 Preferred primers: SEQ ID Primer name NO: Sequence Length_F/Rat2g25320_f 58 ATGAAGCAAAGCTCATCG 21 GAG at3g07610_f 59ATGGATTCTGTGGAGGAAG 22 AAG at3g15600_f 60 ATGGGTATGTGGCTGTTAT 22 TTCat2g25320_r 61 TCATTCTTTTGTGTAGTC 21 TTC at3g07610_r 62CTACATCTTCTCCATTTCTA 23 ATC at3g15600_r 63 TTAAACATTCTTCTTTGTCT 23 TTTTCAt1g14490_f 144 ATGGAAACCGTCGGGCGTCC 24 ACGT At1g14490_r 145TCAGTACGGCGATGGAGCT 22 TTG At1g30570_f 221 ATGTCGAAGCTGAGGAAA 21 AAGAt1g30570_r 222 CTAAGCCGAATTGTGAAGAG 20 At2g21290_f 244ATGGCGGCGATGCAGTGGT 22 GCG At2g2l290_r 245 TCAGATGAGCTTGAAAGGAA 24 GAGGAt3g14230_f 436 ATGTGTGGAGGAGCTATA 21 ATC At3g14230_r 437TCAAAAGTCTCCTTCCAGC 22 ATG

Moreover, it is possible to identify conserved regions from variousorganisms by carrying out protein sequence alignments with thepolypeptide of the invention, in particular with the sequences of shownin SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO:10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ IDNO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71,SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO:81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ IDNO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO:109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO:127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO:152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO:170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO:188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO:206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ IDNO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239,SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ IDNO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262,SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ IDNO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289,SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ IDNO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307,SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ IDNO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325,SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ IDNO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343,SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ IDNO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361,SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ IDNO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379,SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ IDNO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397,SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ IDNO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415,SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ IDNO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433,SEQ ID NO: 435 or SEQ ID NO: 441 from which conserved regions, and inturn, degenerate primers can be derived. Such a conserved region for thepolypeptide of the invention, in particular with the sequences of shownin SEQ ID NO: 33, SEQ ID NO: 34, SEQ ID NO: 146, SEQ ID NO: 147, SEQ IDNO: 148, SEQ ID NO: 149, SEQ ID NO: 150, SEQ ID NO: 223, SEQ ID NO: 224,SEQ ID NO: 225, SEQ ID NO: 226, SEQ ID NO: 227, SEQ ID NO: 246, SEQ IDNO: 247, SEQ ID NO: 248, SEQ ID NO: 273, SEQ ID NO: 274, SEQ ID NO: 275,SEQ ID NO: 276, SEQ ID NO: 277, SEQ ID NO: 278, SEQ ID NO: 279, SEQ IDNO: 280, SEQ ID NO: 281, SEQ ID NO: 438, SEQ ID NO: 439 or SEQ ID NO:442. These degenerate primers can then be utilized by PCR for theamplification of fragments of novel coding regions coding for proteinshaving above-mentioned activity, e.g. conferring the increase of thefine chemical after reducing, decreasing or deleting the expression oractivity of the respective nucleic acid sequence or the protein encodedby said sequence, which having the biological activity of the protein ofthe invention or further functional homologs of the polypeptide of theinvention from other organisms.

These fragments can then be utilized as hybridization probe forisolating the complete gene sequence. As an alternative, the missing 5′and 3′ sequences can be isolated by means of RACE-PCR. A nucleic acidmolecule according to the invention can be amplified using cDNA or, asan alternative, genomic DNA as template and suitable oligonucleotideprimers, following standard PCR amplification techniques. The nucleicacid molecule amplified thus can be cloned into a suitable vector andcharacterized by means of DNA sequence analysis. Oligonucleotides, whichcorrespond to one of the nucleic acid molecules used in the process, canbe generated by standard synthesis methods, for example using anautomatic DNA synthesizer.

Nucleic acid molecules which are advantageously for the processaccording to the invention can be isolated based on their homology tothe nucleic acid molecules disclosed herein using the sequences or partthereof as hybridization probe and following standard hybridizationtechniques under stringent hybridization conditions. In this context, itis possible to use, for example, isolated nucleic acid molecules of atleast 15, 20, 25, 30, 35, 40, 50, 60 or more nucleotides, preferably ofat least 15, 20 or 25 nucleotides in length which hybridize understringent conditions with the above-described nucleic acid molecules, inparticular with those which encompass a nucleotide sequence of SEQ IDNO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ IDNO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31;SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO:72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ IDNO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100,SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ IDNO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118,SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ IDNO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136,SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO:169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO:205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO:230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO:253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO:271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO:316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO:334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO:352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO:370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO:388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO:424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQID NO: 434 or SEQ ID NO: 440. Nucleic acid molecules with 30, 50, 100,250 or more nucleotides may also be used.

the term “homology” means that the respective nucleic acid molecules orencoded proteins are functionally and/or structurally equivalent. Thenucleic acid molecules that are homologous to the nucleic acid moleculesdescribed above and that are derivatives of said nucleic acid moleculesare, for example, variations of said nucleic acid molecules whichrepresent modifications having the same biological function, inparticular encoding proteins with the same or substantially the samebiological function. They may be naturally occurring variations, such assequences from other plant varieties or species, or mutations. Thesemutations may occur naturally or may be obtained by mutagenesistechniques. The allelic variations may be naturally occurring allelicvariants as well as synthetically produced or genetically engineeredvariants. Structurally equivalents can for example be identified bytesting the binding of said polypeptide to antibodies or computer basedpredictions. Structurally equivalent have the similar immunologicalcharacteristic, e.g. comprise similar epitopes.

By “hybridizing” it is meant that such nucleic acid molecules hybridizeunder conventional hybridization conditions, preferably under stringentconditions such as described by, e.g., Sambrook (Molecular Cloning; ALaboratory Manual, 2^(nd) Edition, Cold Spring Harbor Laboratory Press,Cold Spring Harbor, N.Y. (1989)) or in Current Protocols in MolecularBiology, John Wiley & Sons, N.Y. (1989), 6.3.1-6.3.6.

According to the invention, DNA as well as RNA molecules of the nucleicacid of the invention can be used as probes. Further, as template forthe identification of functional homologues Northern blot assays as wellas Southern blot assays can be performed. The Northern blot assayadvantageously provides further informations about the expressed geneproduct: e.g. expression pattern, occurrence of processing steps, likesplicing and capping, etc. The Southern blot assay provides additionalinformation about the chromosomal localization and organization of thegene encoding the nucleic acid molecule of the invention.

A preferred, nonlimiting example of stringent Southern blothydridization conditions are hybridizations in 6× sodium chloride/sodiumcitrate (=SSC) at approximately 45° C., followed by one or more washsteps in 0.2×SSC, 0.1% SDS at 50 to 65° C., for example at 50° C., 55°C. or 60° C. The skilled worker knows that these hybridizationconditions differ as a function of the type of the nucleic acid and, forexample when organic solvents are present, with regard to thetemperature and concentration of the buffer. The temperature under“standard hybridization conditions” differs for example as a function ofthe type of the nucleic acid between 42° C. and 58° C., preferablybetween 45° C. and 50° C. in an aqueous buffer with a concentration of0.1×0.5×, 1×, 2×, 3×, 4× or 5×SSC (pH 7.2). If organic solvent(s) is/arepresent in the abovementioned buffer, for example 50% formamide, thetemperature under standard conditions is approximately 40° C., 42° C. or45° C. The hybridization conditions for DNA:DNA hybrids are preferablyfor example 0.1×SSC and 20° C., 25° C., 30° C., 35° C., 40° C. or 45°C., preferably between 30° C. and 45° C. The hybridization conditionsfor DNA: RNA hybrids are preferably for example 0.1×SSC and 30° C., 35°C., 40° C., 45° C., 50° C. or 55° C., preferably between 45° C. and 55°C. The abovementioned hybridization temperatures are determined forexample for a nucleic acid approximately 100 bp (=base pairs) in lengthand a G+C content of 50% in the absence of formamide. The skilled workerknows to determine the hybridization conditions required with the aid oftextbooks, for example the ones mentioned above, or from the followingtextbooks: Sambrook et al., “Molecular Cloning”, Cold Spring HarborLaboratory, 1989; Hames and Higgins (Ed.) 1985, “Nucleic AcidsHybridization: A Practical Approach”, IRL Press at Oxford UniversityPress, Oxford; Brown (Ed.) 1991, “Essential Molecular Biology: APractical Approach”, IRL Press at Oxford University Press, Oxford.

A further example of one such stringent hybridization condition ishybridization at 4×SSC at 65° C., followed by a washing in 0.1×SSC at65° C. for one hour. Alternatively, an exemplary stringent hybridizationcondition is in 50% formamide, 4×SSC at 42° C. Further, the conditionsduring the wash step can be selected from the range of conditionsdelimited by low-stringency conditions (approximately 2×SSC at 50° C.)and high-stringency conditions (approximately 0.2×SSC at 50° C.,preferably at 65° C.) (20×SSC: 0.3M sodium citrate, 3M NaCl, pH 7.0). Inaddition, the temperature during the wash step can be raised fromlow-stringency conditions at room temperature, approximately 22° C., tohigher-stringency conditions at approximately 65° C. Both of theparameters salt concentration and temperature can be variedsimultaneously, or else one of the two parameters can be kept constantwhile only the other is varied. Denaturants, for example formamide orSDS, may also be employed during the hybridization. In the presence of50% formamide, hybridization is preferably effected at 42° C. Relevantfactors like i) length of treatment, ii) salt conditions, iii) detergentconditions, iv) competitor DNAs, v) temperature and vi) probe selectioncan combined case by case so that not all possibilities can be mentionedherein.

Some examples of conditions for DNA hybridization (Southern blot assays)and wash step are shown hereinbelow:

-   (1) Hybridization conditions can be selected, for example, from the    following conditions:-   a) 4×SSC at 65° C.,-   b) 6×SSC at 45° C.,-   c) 6×SSC, 100 mg/ml denatured fragmented fish sperm DNA at 68° C.,-   d) 6×SSC, 0.5% SDS, 100 mg/ml denatured salmon sperm DNA at 68° C.,-   e) 6×SSC, 0.5% SDS, 100 mg/ml denatured fragmented salmon sperm DNA,    50% formamide at 42° C.,-   f) 50% formamide, 4×SSC at 42° C.,-   g) 50% (vol/vol) formamide, 0.1% bovine serum albumin, 0.1% Ficoll,    0.1% polyvinylpyrrolidone, 50 mM sodium phosphate buffer pH 6.5, 750    mM NaCl, 75 mM sodium citrate at 42° C.,-   h) 2× or 4×SSC at 50° C. (low-stringency condition), or-   i) 30 to 40% formamide, 2× or 4×SSC at 42° C. (low-stringency    condition).-   (2) Wash steps can be selected, for example, from the following    conditions:-   a) 0.015 M NaCl/0.0015 M sodium citrate/0.1% SDS at 50° C.-   b) 0.1×SSC at 65° C.-   c) 0.1×SSC, 0.5% SDS at 68° C.-   d) 0.1×SSC, 0.5% SDS, 50% formamide at 42° C.-   e) 0.2×SSC, 0.1% SDS at 42° C.-   f) 2×SSC at 65° C. (low-stringency condition).-   g) 0.2×SSC, 0.1% SDS at 60° C. (medium-high stringency conditions),    or-   h) 0.1×SSC, 0.1% SDS at 60° C. (medium-high stringency conditions),    or-   l) 0.2×SSC, 0.1% SDS at 65° C. (high stringency conditions), or-   h) 0, 1×SSC, 0.1% SDS at 65° C. (high stringency conditions)

Polypeptides having above-mentioned activity, e.g. conferring the finechemical increase, derived from other organisms, can be encoded by otherDNA sequences, which hybridize to the sequences shown in SEQ ID NO: 1,SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11,SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO:23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ IDNO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82,SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO:92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ IDNO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ IDNO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128,SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ IDNO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151,SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ IDNO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169,SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ IDNO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187,SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ IDNO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205,SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ IDNO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ IDNO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253,SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ IDNO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271,SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ IDNO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298,SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ IDNO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ IDNO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334,SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ IDNO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ IDNO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370,SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ IDNO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388,SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ IDNO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406,SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ IDNO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424,SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ IDNO: 434 or SEQ ID NO: 440 under relaxed hybridization conditions andwhich code on expression for peptides having the further biologicalactivities of the protein of the invention.

Further, some applications have to be performed at low stringencyhybridization conditions, without any consequences for the specificityof the hybridization. For example, a Southern blot analysis of total DNAcould be probed with a nucleic acid molecule of the present inventionand washed at low stringency (55° C. in 2×SSPE, 0.1% SDS). Thehybridisation analysis could reveal a simple pattern of only genesencoding polypeptides of the present invention, e.g. havingherein-mentioned fine chemical increasing activity and/or having alsothe biological activity of the protein of the invention. A furtherexample of such low-stringent hybridization conditions is 4×SSC at 50°C. or hybridization with 30 to 40% formamide at 42° C. Such moleculescomprise those which are fragments, analogues or derivatives of thepolypeptide of the invention and differ, for example, by way of aminoacid and/or nucleotide deletion(s), insertion(s), substitution (s),addition(s) and/or recombination (s) or any other modification(s) knownin the art either alone or in combination from the above-described aminoacid sequences or their underlying nucleotide sequence(s). However, itis preferred to use high stringency hybridisation conditions.

Hybridization should advantageously be carried out with fragments of atleast 5, 10, 15, 20, 25, 30, 35 or 40 bp, advantageously at least 50,60, 70 or 80 bp, preferably at least 90,100 or 110 bp. Most preferablyare fragments of at least 15, 20, or 30 bp. Preferably are alsohybridizations with at least 100 bp or 200, very especially preferablyat least 400 bp in length. In an especially preferred embodiment, thehybridization should be carried out with the entire nucleic acidsequence with conditions described above.

The terms “fragment”, “fragment of a sequence” or “part of a sequence”mean a truncated sequence of the original sequence referred to. Thetruncated sequence (nucleic acid or protein sequence) can vary widely inlength; the minimum size being a sequence of sufficient size to providea sequence or sequence fragment with at least 15, 20, 21, 22, 23, 24,25, 26, 27, 28, 29, 30 bp in length with at least 90, 91, 92, 93, 94,95, 96, 97, 99 or 99% identity preferably 100% identity with a fragmentof the nucleic acid molecules of the invention for example with thesequences shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO:7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: is, SEQ ID NO:19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ IDNO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO:88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ IDNO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106,SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ IDNO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124,SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ IDNO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: ,SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ IDNO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165,SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ IDNO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ IDNO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201,SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ IDNO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ IDNO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249,SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ IDNO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267,SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ IDNO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ IDNO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ IDNO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330,SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ IDNO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348,SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ IDNO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366,SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ IDNO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ IDNO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402,SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ IDNO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ IDNO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440. Saidtruncated sequences can as mentioned vary widely in length from 15 bp upto 2 kb or more, advantageously the sequences have a minimal length of15, 20, 25, 30, 35 or 40 bp, while the maximum size is not critical.100, 200, 300, 400, 500 or more base pair fragments can be used. In someapplications, the maximum size usually is not substantially greater thanthat required to provide the complete gene function(s) of the nucleicacid sequences of the invention. Such sequences can advantageously beenused for the repression, reduction, decrease or deletion of thebiological activity of the nucleic acid molecules and/or proteins of theinvention by for example the RNAi- or antisense-technology. For thereduction, decrease or deletion of the biological activity of theinventive nucleic acid sequence and/or the inventive protein also thepromotor regions of the disclosed nucleic acid sequences can be used.The skilled worker knows how to clone said promotor regions.

Typically, the truncated amino acid sequence will range from about toabout 310 amino acids in length. More typically, however, the sequencewill be a maximum of about 250 amino acids in length, preferably amaximum of about 200 or 100 amino acids. It is usually desirable toselect sequences of at least about 10, 12 or 15 amino acids, up to amaximum of about 20 or 25 amino acids.

The term “epitope” relates to specific immunoreactive sites within anantigen, also known as antigenic determinates. These epitopes can be alinear array of monomers in a polymeric composition—such as amino acidsin a protein—or consist of or comprise a more complex secondary ortertiary structure. Those of skill will recognize that immunogens (i.e.,substances capable of eliciting an immune response) are antigens;however, some antigen, such as haptens, are not immunogens but may bemade immunogenic by coupling to a carrier molecule. The term “antigen”includes references to a substance to which an antibody can be generatedand/or to which the antibody is specifically immunoreactive.

In one embodiment the present invention relates to an epitope of thepolypeptide of the present invention.

The term “one or several amino acids” relates to at least one amino acidbut not more than that number of amino acids, which would result in ahomology of below 50% identity. Preferably, the identity is more than70% or 80%, more preferred are 85%, 90%, 91%, 92%, 93%, 94% or 95%, evenmore preferred are 96%, 97%, 98%, or 99% identity.

Further, the nucleic acid molecule of the invention comprises a nucleicacid molecule, which is a complement of one of the nucleotide sequencesof above mentioned nucleic acid molecules or a portion thereof. Anucleic acid molecule which is complementary to one of the nucleotidesequences shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO:7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO:19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ IDNO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO:88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ IDNO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106,SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ IDNO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124,SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ IDNO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: ,SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ IDNO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165,SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ IDNO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ IDNO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201,SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ IDNO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ IDNO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249,SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ IDNO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267,SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ IDNO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ IDNO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ IDNO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330,SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ IDNO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348,SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ IDNO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366,SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ IDNO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ IDNO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402,SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ IDNO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ IDNO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 is one whichis sufficiently complementary to one of the nucleotide sequences shownin SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9,SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO:21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ IDNO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80,SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO:90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ IDNO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108,SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ IDNO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126,SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ IDNO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ IDNO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167,SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ IDNO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185,SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ IDNO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203,SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ IDNO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228,SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ IDNO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251,SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ IDNO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269,SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ IDNO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296,SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ IDNO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314,SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ IDNO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332,SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ IDNO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350,SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ IDNO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368,SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ IDNO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386,SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ IDNO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404,SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ IDNO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422,SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ IDNO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 such that it can hybridize toone of the nucleotide sequences shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25,SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO:66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ IDNO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94,SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO:104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO:122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO:140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ IDNO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163,SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ IDNO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181,SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ IDNO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199,SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ IDNO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ IDNO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242,SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ IDNO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265,SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ IDNO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292,SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ IDNO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310,SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ IDNO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328,SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ IDNO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346,SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ IDNO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364,SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ IDNO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382,SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ IDNO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400,SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ IDNO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418,SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ IDNO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO:440, thereby forming a stable duplex. Preferably, the hybridisation isperformed under stringent hybridization conditions. However, acomplement of one of the herein disclosed sequences is preferably asequence complement thereto according to the base pairing of nucleicacid molecules well known to the skilled person. For example, the basesA and G undergo base pairing with the bases T and U or C, resp. and viceversa. Modifications of the bases can influence the base-pairingpartner.

The nucleic acid molecule of the invention comprises a nucleotidesequence which is at least about 30%, 35%, 40% or 45%, preferably atleast about 50%, 55%, 60% or 65%, more preferably at least about 70%,80%, or 90%, and even more preferably at least about 95%, 97%, 98%, 99%or more homologous to a nucleotide sequence shown in SEQ ID NO: 1, SEQID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23,SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO:64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ IDNO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92,SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO:102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO:120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO:138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ IDNO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161,SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ IDNO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179,SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ IDNO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197,SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ IDNO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215,SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ IDNO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240,SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ IDNO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263,SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ IDNO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290,SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ IDNO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308,SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ IDNO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326,SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ IDNO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344,SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ IDNO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362,SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ IDNO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380,SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ IDNO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398,SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ IDNO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416,SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ IDNO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434or SEQ ID NO: 440, or a portion thereof and/or has the biologicalactivity of the protein of the invention or the nucleic acid moleculeencoding said protein. The nucleic acid molecule of the inventioncomprises a nucleotide sequence which hybridizes, preferably hybridizesunder stringent conditions as defined herein, to one of the nucleotidesequences shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO:7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO:19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ IDNO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO:88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ IDNO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106,SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ IDNO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124,SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ IDNO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: ,SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ IDNO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165,SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ IDNO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ IDNO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201,SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ IDNO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ IDNO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249,SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ IDNO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267,SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ IDNO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ IDNO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ IDNO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330,SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ IDNO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348,SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ IDNO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366,SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ IDNO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ IDNO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402,SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ IDNO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ IDNO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440, or a portionthereof and encodes a protein having aforementioned activity, e.g.conferring a methionine increase upon the reduction of deletion of itsactivity, and optionally, the biological activity of the protein of theinvention.

Moreover, the nucleic acid molecule of the invention can comprise only aportion of the coding region of one of the sequences in SEQ ID NO: 1,SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11,SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO:23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ IDNO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82,SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO:92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ IDNO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ IDNO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128,SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ IDNO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151,SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ IDNO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169,SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ IDNO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187,SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ IDNO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205,SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ IDNO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ IDNO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253,SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ IDNO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271,SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ IDNO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298,SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ IDNO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ IDNO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334,SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ IDNO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ IDNO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370,SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ IDNO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388,SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ IDNO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406,SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ IDNO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424,SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ IDNO: 434 or SEQ ID NO: 440, for example a fragment which can be used as aprobe or primer or a fragment encoding a biologically active portion ofthe nucleic acid molecule or polypeptide of the present invention or afragment encoding a non active part of the nucleic acid molecule or thepolypeptide of the invention, i.e. having abovementioned activity, e.g.conferring an increase of the fine chemical if its expression oractivity is decreased. The nucleotide sequences determined from thecloning of the present protein according to the invention encoding geneallows for the generation of probes and primers designed for use inidentifying and/or cloning its homologues in other cell types andorganisms. The probe/primer typically comprises substantially purifiedoligonucleotide. The oligonucleotide typically comprises a region ofnucleotide sequence that hybridizes under stringent conditions to atleast about 12, preferably about 20 or 25, more preferably about 40, 50or 75 consecutive nucleotides of a sense strand of one of the sequencesset forth, e.g., in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO:7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO:19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ IDNO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO:88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ IDNO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106,SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ IDNO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124,SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ IDNO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: ,SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ IDNO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165,SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ IDNO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ IDNO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201,SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ IDNO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ IDNO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249,SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ IDNO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267,SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ IDNO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ IDNO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ IDNO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330,SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ IDNO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348,SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ IDNO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366,SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ IDNO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ IDNO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402,SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ IDNO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ IDNO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440, an anti-sensesequence of one of the sequences, e.g., set forth in SEQ ID NO: 1, SEQID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23,SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO:64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ IDNO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92,SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO:102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO:120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO:138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ IDNO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161,SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ IDNO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179,SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ IDNO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197,SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ IDNO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215,SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ IDNO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240,SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ IDNO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263,SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ IDNO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290,SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ IDNO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308,SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ IDNO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326,SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ IDNO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344,SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ IDNO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362,SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ IDNO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380,SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ IDNO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398,SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ IDNO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416,SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ IDNO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434or SEQ ID NO: 440, or naturally occurring mutants thereof. Primers basedon a nucleotide of invention can be used in PCR reactions to clonehomologues of the polypeptide of the invention, e.g. as the primersdescribed in the examples of the present invention, e.g. as shown in theexamples. Said nucleic acid molecules, which are homologues of thesequence of the invention or the nucleic acid molecules of the inventionthemselves can be used to reduce, decrease or delete the biologicalactivity of the nucleic acid molecules and/or proteins of the invention.

Primer sets are interchangeable. The person skilled in the art knows tocombine said primers to result in the desired product, e.g. in afull-length clone or a partial sequence. Probes based on the sequencesof the nucleic acid molecule of the invention can be used to detecttranscripts or genomic sequences encoding the same or homologousproteins. The probe can further comprise a label group attached thereto,e.g. the label group can be a radioisotope, a fluorescent compound, anenzyme, or an enzyme co-factor. Such probes can be used as a part of agenomic marker test kit for identifying cells which express or does notexpress a polypeptide of the invention, such as by measuring a level ofan encoding nucleic acid molecule in a sample of cells, e.g., detectingmRNA levels or determining, whether a genomic gene comprising thesequence of the polynucleotide of the invention has been mutated ordeleted.

The nucleic acid molecule of the invention encodes a polypeptide orportion thereof which includes an amino acid sequence which issufficiently homologous to the amino acid sequence of SEQ ID NO: 2, SEQID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24,SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ IDNO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93,SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO:121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO:164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO:182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO:218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO:266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO:293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO:329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO:365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO:383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO:419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:441 such that the protein or portion thereof maintains the ability toparticipate in the fine chemical production, in particular a compoundsuch as the protein of the invention which is involved in the reduction,decrease and/or deletion of the fine chemical by for examplemetabolization, degradation or export of undisered chemical compoundsand thereby increasing the production of the fine chemical upon thereduction or deletion of its activity as mentioned above or as describedin the examples in plants or microorganisms is comprised.

As used herein, the language “sufficiently homologous” refers toproteins or portions thereof which have amino acid sequences whichinclude a minimum number of identical or equivalent amino acid residues(e.g., an amino acid residue which has a similar side chain as an aminoacid residue in one of the sequences of the polypeptide of the presentinvention) to an amino acid sequence shown in SEQ ID NO: 2, SEQ ID NO:4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ IDNO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75,SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO:85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ IDNO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103,SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ IDNO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ IDNO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139,SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ IDNO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ IDNO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182,SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ IDNO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ IDNO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO:235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO:258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO:285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO:303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO:321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO:339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO:375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO:411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO:429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441.Portions of the aforementioned amino acid sequence are at least 3, 5,10, 20, 30, 40, 50 or more amino acids in length. Nucleic acidsequences, which as a result of the degeneracy of the genetic code, canbe derived from said polypeptide sequences can be used for therepression, decrease or deletion of the biological activity of thepolypeptide or the nucleic acid molecule of the invention according tothe disclosure herein.

In one embodiment, the nucleic acid molecule of the present inventioncomprises a nucleic acid that encodes a portion of the protein of thepresent invention. The protein is at least about 30%, 35%, 40%, 45% or50%, preferably at least about 55%, 60%, 65% or 70% and more preferablyat least about 75%, 80%, 85%, 90%, 91%, 92%, 93% or 94% and mostpreferably at least about 95%, 97%, 98%, 99% or more homologous to anentire amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 and havingabove-mentioned activity, e.g. conferring preferably the increase of thefine chemical.

Portions of proteins encoded by the nucleic acid molecule of theinvention are preferably in such a manner biologically active, that theyare increasing the productivity to produce the fine chemical by being inits biological activity reduced, decreased or deleted.

As mentioned herein, the term “biologically active portion” is intendedto include a portion, e.g., a domain/motif, that confers by introducingsaid nucleic acid sequence or part thereof an increase of the productionof the fine chemical.

The invention further relates to nucleic acid molecules which are usedin the inventive process and which as a result of degeneracy of thegenetic code can be derived from a polypeptide sequence as depicted inSEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10,SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ IDNO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81,SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO:91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ IDNO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ IDNO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127,SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ IDNO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152,SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ IDNO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170,SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ IDNO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188,SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ IDNO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206,SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ IDNO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231,SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ IDNO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254,SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ IDNO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272,SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ IDNO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ IDNO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317,SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ IDNO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335,SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ IDNO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353,SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ IDNO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371,SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ IDNO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389,SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ IDNO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407,SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ IDNO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425,SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ IDNO: 435 or SEQ ID NO: 441 and thus encoding a polypeptide of the presentinvention, in particular a polypeptide leading by reducing, decreasingor deleting its biological activity to an increase in the fine chemicalin an organism. Advantageously, the nucleic acid molecule of theinvention comprises, or in an other embodiment has, a nucleotidesequence encoding a protein comprising, or in an other embodimenthaving, an amino acid sequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26,SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO:67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ IDNO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95,SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO:105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO:123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO:141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO:166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO:184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO:220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO:250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO:268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO:295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO:331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO:349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO:385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO:403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO:421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441, whichdiffer from said amino acid sequences in at least one or more aminoacids.

In addition, it will be appreciated by those skilled in the art that DNAsequence polymorphisms that lead to changes in the amino acid sequencesmay exist within a population. Such genetic polymorphism in the geneencoding the polypeptide of the invention or comprising the nucleic acidmolecule of the invention may exist among individuals within apopulation due to natural variation.

As used herein, the terms “gene” and “recombinant gene” refer to nucleicacid molecules comprising an open reading frame encoding the polypeptideof the invention or comprising the nucleic acid molecule of theinvention, preferably encoding the polypeptide of the invention orcomprising the nucleic acid molecule of the invention derived from acrop plant or from a microorganism useful for the production of finechemicals, in particular encoding the polypeptide of the invention orcomprising the nucleic acid molecule of the invention derived from acrop plant or a microorganism for the production of the fine chemical.Such natural variations can typically result in 1-5% variance in thenucleotide sequence of the gene used in the inventive process. Any andall such nucleotide variations and resulting amino acid polymorphisms ingenes encoding a polypeptide of the invention or comprising the nucleicacid molecule of the invention that are the result of natural variationand that in the event of reducing, decreasing or deleting its biologicalactivity do not alter the functional activity as described are intendedto be within the scope of the invention.

Nucleic acid molecules corresponding to natural variants homologues of anucleic acid molecule of the invention, which can also be a cDNA, can beisolated based on their homology to the nucleic acid molecules disclosedherein using the nucleic acid molecule of the invention, or a portionthereof, as a hybridization probe according to standard hybridizationtechniques under stringent hybridization conditions.

Accordingly, in another embodiment, a nucleic acid molecule of theinvention is at least 15, 20, 25 or 30 nucleotides in length.Preferably, it hybridizes under stringent conditions to a nucleic acidmolecule comprising a nucleotide sequence of the nucleic acid moleculeof the present invention, e.g. comprising the sequence shown in SEQ IDNO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ IDNO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31;SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO:72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ IDNO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100,SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ IDNO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118,SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ IDNO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136,SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO:169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO:205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO:230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO:253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO:271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO:316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO:334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO:352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO:370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO:388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO:424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQID NO: 434 or SEQ ID NO: 440. The nucleic acid molecule is preferably atleast 20, 30, 50, 100, 250 or more nucleotides in length.

The term “hybridizes under stringent conditions” is defined above. Inone embodiment, the term “hybridizes under stringent conditions” isintended to describe conditions for hybridization and washing underwhich nucleotide sequences of at least 30%, 40%, 50% or 65% identical toeach other typically remain hybridized to each other. Preferably, theconditions are such that sequences of at least about 70%, morepreferably at least about 75% or 80%, and even more preferably of atleast about 85%, 90% or 95% or more identical to each other typicallyremain hybridized to each other.

Preferably, nucleic acid molecule of the invention that hybridizes understringent conditions to a sequence of SEQ ID NO: 1, SEQ ID NO: 3, SEQ IDNO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ IDNO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQID NO: 27, SEQ ID NO: 29 or SEQ ID NO: 31 corresponds to anaturally-occurring nucleic acid molecule of the invention. As usedherein, a “naturally-occurring” nucleic acid molecule refers to a RNA orDNA molecule having a nucleotide sequence that occurs in nature (e.g.,encodes a natural protein). Preferably, the nucleic acid moleculeencodes a natural protein having above-mentioned activity, e.g.conferring increase of the fine chemical after reducing, decreasing ordeleting the expression or activity thereof or the activity of a proteinhaving the biological activity of the protein of the invention.

In addition to naturally-occurring variants of the nucleic acid orprotein sequence of the invention that may exist in the population, theskilled artisan will further appreciate that changes can be introducedby mutation into a nucleotide sequence of the nucleic acid moleculeencoding the polypeptide of the invention, thereby leading to changes inthe amino acid sequence of the encoded polypeptide of the invention andthereby altering the functional ability of the polypeptide, meaningpreferably reducing, decreasing or deleting said activity. For example,nucleotide substitutions leading to amino acid substitutions at“essential” amino acid residues can be made in a sequence of the nucleicacid molecule of the invention, e.g. in SEQ ID NO: 1, SEQ ID NO: 3, SEQID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25,SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO:66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ IDNO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 94, SEQID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94,SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO:104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO:122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO:140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ IDNO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163,SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ IDNO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181,SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ IDNO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199,SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ IDNO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ IDNO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242,SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ IDNO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265,SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ IDNO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292,SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ IDNO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310,SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ IDNO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328,SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ IDNO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346,SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ IDNO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364,SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ IDNO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382,SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ IDNO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400,SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ IDNO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418,SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ IDNO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO:440. An “essential” amino acid residue is a residue that if altered fromthe wild-type sequence of one of the polypeptide of the invention leadto an altered activity of said polypeptide, whereas a “non-essential”amino acid residue is not required for the activity of the protein forexample for the activity as an enzyme. The alteration of “essential”residues lead to a reduced decreased or deleted activity of thepolypeptides of the invention. Preferably amino acids of the polypeptideof the invention are change in such a manner that the activity isreduced, decreased or deleted that means preferably essential amino acidresidues and/or more non-essential residues are changed and thereby theactivity is reduced, which leads as mentioned above to an increase inthe fine chemical in an organism after decreasing the expression oractivity of the polypeptide of the invention. Other amino acid residues,however, (e.g., those that are not conserved or only semiconserved inthe domain having said activity) may not be essential for activity andthus are likely to be amenable to alteration without altering saidactivity are less preferred.

Further, a person skilled in the art knows that the codon usage betweenorganisms can differ. Therefore, he will adapt the codon usage in thenucleic acid molecule of the present invention to the usage of theorganism in which the polynucleotide or polypeptide is expressed, sothat the expression of the nucleic acid molecule or the encoded proteinof the invention is more likely reduced.

Accordingly, the invention relates to nucleic acid molecules encodingpolypeptide having abovementioned activity, e.g. conferring an increasein the fine chemical content in an organism or parts thereof thatcontain changes in amino acid residues that are essential for saidactivity. Such polypeptides differ in amino acid sequence from asequence contained in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ IDNO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ IDNO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ IDNO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115,SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ IDNO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ IDNO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ IDNO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ IDNO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ IDNO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ IDNO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ IDNO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ IDNO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ IDNO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305,SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ IDNO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ IDNO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ IDNO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ IDNO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ IDNO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ IDNO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ IDNO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 yet do not retain saidbiological activity described herein and thereby enabling the finechemical production. The nucleic acid molecule can comprise a nucleotidesequence encoding a polypeptide, wherein the polypeptide comprises anamino acid sequence at least about 50% identical to an amino acidsequence of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20,SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO.30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ IDNO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89,SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO:99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO:117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO:135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO:160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO:178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO:196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO:214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO:239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO:262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO:289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO:307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO:343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO:361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO:379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO:397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO:415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO:433, SEQ ID NO: 435 or SEQ ID NO: 441 and is capable of participation inthe increase of the fine chemical production after decreasing itsexpression or its biological function. Preferably, the protein encodedby the nucleic acid molecule is at least about 60%, 70% or 80% identicalto the sequence in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ IDNO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ IDNO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO:107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO:297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441, more preferably at leastabout 85% identical to one of the sequences in SEQ ID NO: 2, SEQ ID NO:4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO:14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ IDNO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75,SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO:85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ IDNO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103,SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ IDNO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ IDNO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139,SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ IDNO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ IDNO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182,SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ IDNO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ IDNO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO:235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO:258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO:285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO:303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO:321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO:339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO:375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO:411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO:429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441,even more preferably at least about 90%, 91%, 92%, 93%, 94%, 95%homologous to the sequence in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441, and mostpreferably at least about 96%, 97%, 98%, or 99% identical to thesequence in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20,SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO.30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ IDNO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89,SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO:99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO:117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO:135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO:160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO:178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO:196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO:214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO:239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO:262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO:289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO:307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO:343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO:361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO:379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO:397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO:415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO:433, SEQ ID NO: 435 or SEQ ID NO: 441.

To determine the percentage homology (=identity) of two amino acidsequences (for example of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16,SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO:28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ IDNO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87,SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO:97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO:133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO:158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO:176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO:194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO:212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441) or of two nucleicacid molecules (for example of the sequence SEQ ID NO: 1, SEQ ID NO: 3,SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13,SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO:25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ IDNO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84,SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO:94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ IDNO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112,SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ IDNO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130,SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ IDNO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153,SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ IDNO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171,SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ IDNO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189,SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ IDNO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207,SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ IDNO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232,SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ IDNO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255,SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ IDNO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282,SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ IDNO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300,SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ IDNO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318,SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ IDNO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336,SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ IDNO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354,SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ IDNO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372,SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ IDNO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390,SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ IDNO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408,SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ IDNO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426,SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ IDNO: 440), the sequences are written one underneath the other for anoptimal comparison (for example gaps may be inserted into the sequenceof a protein or of a nucleic acid in order to generate an optimalalignment with the other protein or the other nucleic acid). The aminoacid residues or nucleic acid molecules at the corresponding amino acidpositions or nucleotide positions are then compared. If a position inone sequence is occupied by the same amino acid residue or the samenucleic acid molecule as the corresponding position in the othersequence, the molecules are homologous at this position. (i.e. aminoacid or nucleic acid “homology” as used in the present contextcorresponds to amino acid or nucleic acid “identity”. The percentagehomology between the two sequences is a function of the number ofidentical positions shared by the sequences (i.e. % homology=number ofidentical positions/total number of positions×100). The terms “homology”and “identity” are thus to be considered as synonyms for thisdescription.

For the determination of the percentage homology (=identity) of two ormore amino acids or of two or more nucleotide sequences several computersoftware programs have been developed. The homology of two or moresequences can be calculated with for example the software fasta, whichpresently has been used in the version fasta 3 (W. R. Pearson and D. J.Lipman (1988), Improved Tools for Biological Sequence Comparison. PNAS85:2444-2448; W. R. Pearson (1990) Rapid and Sensitive SequenceComparison with FASTP and FASTA, Methods in Enzymology 183:63-98; W. R.Pearson and D. J. Lipman (1988) Improved Tools for Biological SequenceComparison. PNAS 85:2444-2448; W. R. Pearson (1990); Rapid and SensitiveSequence Comparison with FASTP and FASTAMethods in Enzymology183:63-98). Another useful program for the calculation of homologies ofdifferent sequences is the standard blast program, which is included inthe Biomax pedant software (Biomax, Munich, Federal Republic ofGermany). This leads unfortunately sometimes to suboptimal results sinceblast does not always include complete sequences of the subject and thequerry. Nevertheless as this program is very efficient it can be usedfor the comparison of a huge number of sequences. The following settingsare typically used for such a comparisons of sequences:

−p Program Name [String]; −d Database [String]; default=nr; −i QueryFile [File In]; default=stdin; −e Expectation value (E) [Real];default=10.0; −m alignment view options: 0=pairwise; 1=query-anchoredshowing identities; 2=query-anchored no identities; 3=flatquery-anchored, show identities; 4=flat query-anchored, no identities;5=query-anchored no identities and blunt ends; 6=flat query-anchored, noidentities and blunt ends; 7=XML Blast output; 8=tabular; 9 tabular withcomment lines [Integer]; default=0; −o BLAST report Output File [FileOut] Optional; default=stdout; −F Filter query sequence (DUST withblastn, SEG with others) [String]; default=T; −G Cost to open a gap(zero invokes default behavior) [Integer]; default=0; −E Cost to extenda gap (zero invokes default behavior) [Integer]; default=0; −X X dropoffvalue for gapped alignment (in bits) (zero invokes default behavior);blastn 30, megablast 20, tblastx 0, all others 15 [Integer]; default=0;−I Show GI's in deflines [T/F]; default=F; −q Penalty for a nucleotidemismatch (blastn only) [Integer]; default=−3; −r Reward for a nucleotidematch (blastn only) [Integer]; default=1; −v Number of databasesequences to show one-line descriptions for (V) [Integer]; default=500;−b Number of database sequence to show alignments for (B) [Integer];default=250; −f Threshold for extending hits, default if zero; blastp11, blastn 0, blastx 12, tblastn 13; tblastx 13, megablast 0 [Integer];default=0; −g Perform gapped alignment (not available with tblastx)[T/F]; default=T; −Q Query Genetic code to use [Integer]; default=1; −DDB Genetic code (for tblast[nx] only) [Integer]; default=1; −a Number ofprocessors to use [Integer]; default=1; −O SeqAlign file [File Out]Optional; −J Believe the query defline [T/F]; default=F; −M Matrix[String]; default=BLOSUM62; −W Word size, default if zero (blastn 11,megablast 28, all others 3) [Integer]; default=0; −z Effective length ofthe database (use zero for the real size) [Real]; default=0; −K Numberof best hits from a region to keep (off by default, if used a value of100 is recommended) [Integer]; default=0; −P 0 for multiple hit, 1 forsingle hit [Integer]; default=0; −Y Effective length of the search space(use zero for the real size) [Real]; default=0; −S Query strands tosearch against database (for blast[nx], and tblastx); 3 is both, 1 istop, 2 is bottom [Integer]; default=3; −T Produce HTML output [T/F];default=F; −I Restrict search of database to list of GI's [String]Optional; −U Use lower case filtering of FASTA sequence [T/F] Optional;default=F; −y X dropoff value for ungapped extensions in bits (0.0invokes default behavior); blastn 20, megablast 10, all others 7 [Real];default=0.0; −Z X dropoff value for final gapped alignment in bits (0.0invokes default behavior); blastn/megablast 50, tblastx 0, all others 25[Integer]; default=0; −R PSI-TBLASTN checkpoint file [File In] Optional;−n MegaBlast search [T/F]; default=F; −L Location on query sequence[String] Optional; −A Multiple Hits window size, default if zero(blastn/megablast 0, all others 40 [Integer]; default=0; −w Frame shiftpenalty (OOF algorithm for blastx) [Integer]; default=0; −t Length ofthe largest intron allowed in tblastn for linking HSPs (0 disableslinking) [Integer]; default=0.

Results of high quality are reached by using the algorithm of Needlemanand Wunsch or Smith and Waterman. Therefore programs based on saidalgorithms are preferred. Advantageously the comparisons of sequencescan be done with the program PileUp (J. Mol. Evolution., 25, 351-360,1987, Higgins et al., CABIOS, 5 1989: 151-153) or preferably with theprograms Gap and BestFit, which are respectively based on the algorithmsof Needleman and Wunsch [J. Mol. Biol. 48; 443-453 (1970)] and Smith andWaterman [Adv. Appl. Math. 2; 482-489 (1981)]. Both programs are part ofthe GCG software-package [Genetics Computer Group, 575 Science Drive,Madison, Wis., USA 53711 (1991); Altschul et al. (1997) Nucleic AcidsRes. 25:3389 et seq.]. Therefore preferably the calculations todetermine the percentages of sequence homology are done with the programGap over the whole range of the sequences. The following standardadjustments for the comparison of nucleic acid sequences were used: gapweight: 50, length weight: 3, average match: 10.000, average mismatch:0.000.

For example a sequence which has a 80% homology with sequence SEQ ID NO:1, SEQ ID NO: 7, SEQ ID NO: 19, SEQ ID NO: 65, SEQ ID NO: 152, SEQ IDNO: 229 or SEQ ID NO: 283 at the nucleic acid level is understood asmeaning a sequence which, upon comparison with the sequence SEQ ID NO:1, SEQ ID NO: 7, SEQ ID NO: 19, SEQ ID NO: 65, SEQ ID NO: 152, SEQ IDNO: 229 or SEQ ID NO: 283 by the above Gap program algorithm with theabove parameter set, has 80% homology.

Homology between two polypeptides is understood as meaning the identityof the amino acid sequence over in each case the entire sequence lengthwhich is calculated by comparison with the aid of the program algorithmGap (Wisconsin Package Version 10.0, University of Wisconsin, GeneticsComputer Group (GCG), Madison, USA), setting the following parameters:gap weight: 8; length weight: 2; average match: 2.912; average mismatch:−2.003.

For example a sequence which has a 80% homology with sequence SEQ ID NO:2, SEQ ID NO: 8, SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO: 152, SEQ IDNO: 229 or SEQ ID NO: 283 at the protein level is understood as meaninga sequence which, upon comparison with the sequence SEQ ID NO: 2, SEQ IDNO: 8, SEQ ID NO: 20, SEQ ID NO: 65, SEQ ID NO: 152, SEQ ID NO: 229 orSEQ ID NO: 283 by the above Gap program algorithm with the aboveparameter set, has 80% homology.

Functional equivalents derived from one of the polypeptides as shown inSEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10,SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ IDNO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81,SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO:91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ IDNO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ IDNO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127,SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ IDNO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152,SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ IDNO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170,SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ IDNO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188,SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ IDNO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206,SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ IDNO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231,SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ IDNO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254,SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ IDNO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272,SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ IDNO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ IDNO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317,SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ IDNO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335,SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ IDNO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353,SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ IDNO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371,SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ IDNO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389,SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ IDNO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407,SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ IDNO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425,SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ IDNO: 435 or SEQ ID NO: 441 according to the invention by substitution,insertion or deletion have at least 30%, 35%, 40%, 45% or 50%,preferably at least 55%, 60%, 65% or 70% by preference at least 80%,especially preferably at least 85% or 90%, 91%, 92%, 93% or 94%, veryespecially preferably at least 95%, 97%, 98% or 99% homology with one ofthe polypeptides as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 according to theinvention and are distinguished by essentially the same properties asthe polypeptide as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 preferably of thepolypeptides of S. cerevisiae, E. coli or A. thaliana.

Functional equivalents derived from the nucleic acid sequence as shownin SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9,SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO:21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ IDNO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80,SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO:90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ IDNO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108,SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ IDNO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126,SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ IDNO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ IDNO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167,SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ IDNO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185,SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ IDNO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203,SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ IDNO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228,SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ IDNO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251,SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ IDNO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269,SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ IDNO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296,SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ IDNO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314,SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ IDNO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332,SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ IDNO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350,SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ IDNO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368,SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ IDNO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386,SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ IDNO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404,SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ IDNO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422,SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ IDNO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 according to the invention bysubstitution, insertion or deletion have at least 30%, 35%, 40%, 45% or50%, preferably at least 55%, 60%, 65% or 70% by preference at least80%, especially preferably at least 85% or 90%, 91%, 92%, 93% or 94%,very especially preferably at least 95%, 97%, 98% or 99% homology withone of the polypeptides as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ IDNO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ IDNO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67,SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO:77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ IDNO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO:105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO:123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO:141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO:166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO:184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO:220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO:250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO:268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQID NO: 297, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO:295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO:331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO:349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO:385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO:403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO:421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 accordingto the invention and encode polypeptides having essentially the sameproperties as the polypeptide as shown in SEQ ID NO: 2, SEQ ID NO: 4,SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO:26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ IDNO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85,SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO:95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ IDNO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113,SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ IDNO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131,SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ IDNO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156,SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ IDNO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174,SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ IDNO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192,SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ IDNO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ IDNO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235,SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ IDNO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258,SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ IDNO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285,SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ IDNO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303,SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ IDNO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321,SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ IDNO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339,SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ IDNO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357,SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ IDNO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375,SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ IDNO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393,SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ IDNO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411,SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ IDNO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429,SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441.

“Essentially the same properties” of a functional equivalent is aboveall understood as meaning that the functional equivalent has abovementioned activity, e.g conferring an increasing in the fine chemicalamount while decreasing the amount of protein, activity or function ofsaid functional equivalent in an organism, e.g. a microorganism, a plantor in a plant or animal tissue, plant or animal cells or a part of thesame.

A nucleic acid molecule encoding an homologous to a protein sequence ofSEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10,SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ IDNO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81,SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO:91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ IDNO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109,SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ IDNO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127,SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ IDNO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152,SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ IDNO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170,SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ IDNO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188,SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ IDNO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206,SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ IDNO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231,SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ IDNO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254,SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ IDNO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272,SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ IDNO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299,SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ IDNO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317,SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ IDNO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335,SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ IDNO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353,SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ IDNO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371,SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ IDNO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389,SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ IDNO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407,SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ IDNO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425,SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ IDNO: 435 or SEQ ID NO: 441 can be created by introducing one or morenucleotide substitutions, additions or deletions into a nucleotidesequence of the nucleic acid molecule of the present invention, inparticular of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8,SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO:20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ IDNO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79,SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO:89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ IDNO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107,SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ IDNO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125,SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ IDNO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143,SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ IDNO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168,SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ IDNO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186,SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ IDNO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204,SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ IDNO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229,SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO:239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO:262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO:289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO:307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO:325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO:343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO:361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO:379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO:397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO:415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO:433, SEQ ID NO: 435 or SEQ ID NO: 441 such that one or more amino acidsubstitutions, additions or deletions are introduced into the encodedprotein. Mutations can be introduced into the sequences of, e.g. SEQ IDNO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ IDNO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31;SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO:72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ IDNO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100,SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ IDNO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118,SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ IDNO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136,SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO:169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO:205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO:230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO:253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO:271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO:316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO:334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO:352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO:370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO:388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO:424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQID NO: 434 or SEQ ID NO: 440 by standard techniques, such assite-directed mutagenesis and PCR-mediated mutagenesis.

Preferably, non-conservative amino acid substitutions are made at one ormore predicted non-essential or preferably essential amino acid residuesand thereby reducing, decreasing or deleting the activity of therespective protein. A “conservative amino acid substitution” is one inwhich the amino acid residue is replaced with an amino acid residuehaving a similar side chain. Families of amino acid residues havingsimilar side chains have been defined in the art. These families includeamino acids with basic side chains (e.g., lysine, arginine, histidine),acidic side chains (e.g., aspartic acid, glutamic acid), uncharged polarside chains (e.g., glycine, asparagine, glutamine, serine, threonine,tyrosine, cysteine), nonpolar side chains (e.g., alanine, valine,leucine, isoleucine, proline, phenylalanine, methionine, tryptophan),beta-branched side chains (e.g., threonine, valine, isoleucine) andaromatic side chains (e.g., tyrosine, phenylalanine, tryptophan,histidine).

Thus, a predicted nonessential or essential amino acid residue in apolypeptide of the invention is preferably replaced with another aminoacid residue from another family. Alternatively, in another embodiment,mutations can be introduced randomly along all or part of a codingsequence of a nucleic acid molecule of the invention, such as bysaturation mutagenesis, and the resultant mutants can be screened foractivity described herein to identify mutants that lost or havedecreased biological activity, e.g. conferring an increase in the finechemical content.

Following mutagenesis of one of the sequences of SEQ ID NO: 1, SEQ IDNO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ IDNO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64,SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO:74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ IDNO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102,SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ IDNO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120,SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ IDNO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138,SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO:153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO:171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO:189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO:207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO:232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO:255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO:282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO:300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO:318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO:336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO:354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO:372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO:390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO:408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO:426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 orSEQ ID NO: 440 the encoded protein can be expressed recombinantly andthe activity of the protein can be determined using, for example, assaysdescribed herein (see Examples).

The highest homology of the nucleic acid molecule used in the processaccording to the invention was found for the following database entriesby Gap search:

Hit Best Homolog % Identity At2g25320 (RefSeq At2g25330 35 AccessionNM_128089) At3g07610 (RefSeq NP_172659 33 Accession NP_187418) at3g15600 (RefSeq NP_178820.1 83 Accession NM_112428.1) At1g 14490 (RefSeqSPTREMBL_Q9LTA2 69 Accession NM_101316) At1g30570 (RefSeqSPTREMBL_Q7XTT9 53 Accession NM_102794) At2g21290 (RefSeq bn43069381 85Accession NM_127701) At3g 14230 (RefSeq REMTREMBL_(—) 98 AccessionNM_180251) CAD88898

Homologues of the nucleic acid sequences used, with the sequence SEQ IDNO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ IDNO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31;SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO:72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ IDNO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100,SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ IDNO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118,SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ IDNO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136,SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO:169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO:205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO:230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO:253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO:271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO:316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO:334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO:352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO:370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO:388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO:406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO:424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQID NO: 434 or SEQ ID NO: 440 or of the nucleic acid sequences derivedfrom the sequences SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ IDNO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ IDNO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO:107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO:297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 comprise also allelicvariants with at least approximately 30%, 35%, 40% or 45% homology, bypreference at least approximately 50%, 60% or 70%, more preferably atleast approximately 90%, 91%, 92%, 93%, 94% or 95% and even morepreferably at least approximately 96%, 97%, 98%, 99% or more homologywith one of the nucleotide sequences shown or the abovementioned derivednucleic acid sequences or their homologues, derivatives or analogues orparts of these. Allelic variants encompass in particular functionalvariants which can be obtained by deletion, insertion or substitution ofnucleotides from the sequences shown, preferably from SEQ ID NO: 1, SEQID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23,SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO:64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ IDNO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92,SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO:102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO:120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO:138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ IDNO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161,SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ IDNO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179,SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ IDNO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197,SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ IDNO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215,SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ IDNO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240,SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ IDNO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263,SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ IDNO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290,SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ IDNO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308,SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ IDNO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326,SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ IDNO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344,SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ IDNO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362,SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ IDNO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380,SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ IDNO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398,SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ IDNO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416,SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ IDNO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434or SEQ ID NO: 440, or from the derived nucleic acid sequences, theintention being, however, that the enzyme activity or the biologicalactivity of the resulting proteins synthesized is advantageously lost ordecreased.

In one embodiment of the present invention, the nucleic acid moleculecomprises the sequences shown in any of the SEQ ID NO: 1, SEQ ID NO: 3,SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13,SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO:25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ IDNO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84,SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO:94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ IDNO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112,SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ IDNO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130,SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ IDNO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153,SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ IDNO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171,SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ IDNO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189,SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ IDNO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207,SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ IDNO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232,SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ IDNO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255,SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ IDNO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282,SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ IDNO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300,SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ IDNO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318,SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ IDNO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336,SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ IDNO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354,SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ IDNO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372,SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ IDNO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390,SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ IDNO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408,SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ IDNO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426,SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ IDNO: 440. It is preferred that the nucleic acid molecule comprises aslittle as possible other nucleotides not shown in any one of SEQ ID NO:1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO:11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ IDNO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72,SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO:82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ IDNO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO:110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO:128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151,SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ IDNO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169,SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ IDNO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187,SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ IDNO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205,SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ IDNO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ IDNO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253,SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ IDNO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271,SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ IDNO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298,SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ IDNO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ IDNO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334,SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ IDNO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ IDNO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370,SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ IDNO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388,SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ IDNO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406,SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ IDNO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424,SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ IDNO: 434 or SEQ ID NO: 440. In one embodiment, the nucleic acid moleculecomprises less than 500, 400, 300, 200, 100, 90, 80, 70, 60, 50 or 40further nucleotides. In a further embodiment, the nucleic acid moleculecomprises less than 30, 20 or 10 further nucleotides. In one embodiment,the nucleic acid molecule use in the process of the invention isidentical to the sequences shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ IDNO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ IDNO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66,SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO:76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ IDNO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO:104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO:122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO:140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ IDNO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163,SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ IDNO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181,SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ IDNO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199,SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ IDNO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ IDNO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242,SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ IDNO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265,SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ IDNO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292,SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ IDNO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310,SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ IDNO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328,SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ IDNO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346,SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ IDNO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364,SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ IDNO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382,SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ IDNO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400,SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ IDNO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418,SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ IDNO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO:440.

Also preferred is that the nucleic acid molecule of the inventionencodes a polypeptide comprising the sequence shown in SEQ ID NO: 2, SEQID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24,SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ IDNO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93,SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO:121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO:164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO:182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO:218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO:266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO:293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO:329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO:365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO:383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO:419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:441. In one embodiment, the nucleic acid molecule encodes less than 150,130, 100, 80, 60, 50, 40 or 30 further amino acids. In a furtherembodiment, the encoded polypeptide comprises less than 20, 15, 10, 9,8, 7, 6 or 5 further amino acids. In one embodiment used in theinventive process, the encoded polynucleotide is identical to thesequences shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ IDNO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ IDNO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO:107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO:297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441.

In one embodiment, the nucleic acid molecule encoding a polypeptidecomprising the sequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO:6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 comprises lessthan 100 further nucleotides. In a further embodiment, the nucleic acidmolecule comprises less than 30 further nucleotides. In one embodiment,the nucleic acid molecule is identical to a coding sequence of thesequences shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ IDNO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ IDNO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO:107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO:297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441.

Polypeptides (=proteins), which still have the abovementioned activityof the polypeptide of the present invention, e.g. conferring an increaseof the fine chemical or having the biological activity of the protein ofthe invention, i.e. polypeptides whose activity is essentially reduced,are polypeptides by at least 10% or 20%, by preference 30% or 40%,especially preferably 50% or 60%, very especially preferably 80% or 90%or more in comparison to the wild type biological activity or enzymeactivity, advantageously, the activity is essentially reduced incomparison with the activity of a protein encoded by SEQ ID NO: 2, SEQID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20 SEQ ID NO: 22, SEQ ID NO: 24,SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ IDNO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93,SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO:121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO:164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO:182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO:218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO:266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO:293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO:329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO:365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO:383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO:419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:441 and expressed under identical conditions.

Homologues of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7,SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO:19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ IDNO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO:88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ IDNO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106,SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ IDNO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124,SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ IDNO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: ,SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ IDNO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165,SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ IDNO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ IDNO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201,SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ IDNO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219,SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ IDNO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249,SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ IDNO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267,SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ IDNO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294,SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ IDNO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312,SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ IDNO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330,SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ IDNO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348,SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ IDNO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366,SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ IDNO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ IDNO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402,SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ IDNO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ IDNO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or of thederived sequences of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ IDNO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ IDNO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ IDNO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115,SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ IDNO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ IDNO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ IDNO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ IDNO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ IDNO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ IDNO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ IDNO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ IDNO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ IDNO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305,SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ IDNO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ IDNO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ IDNO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ IDNO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ IDNO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ IDNO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ IDNO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 also mean truncatedsequences, cDNA, single-stranded DNA or RNA of the coding and noncodingDNA sequence. Homologues of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5,SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15,SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO:27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ IDNO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86,SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO:96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO:114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO:132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155,SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ IDNO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173,SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ IDNO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191,SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ IDNO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209,SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ IDNO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234,SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ IDNO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257,SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ IDNO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284,SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ IDNO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302,SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ IDNO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320,SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ IDNO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338,SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ IDNO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356,SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ IDNO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374,SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ IDNO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392,SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ IDNO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410,SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ IDNO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428,SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440 or thederived sequences of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ IDNO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ IDNO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ IDNO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115,SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ IDNO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ IDNO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ IDNO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ IDNO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ IDNO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ IDNO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ IDNO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ IDNO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ IDNO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305,SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ IDNO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ IDNO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ IDNO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ IDNO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ IDNO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ IDNO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ IDNO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 are also understood asmeaning derivatives which comprise noncoding regions such as, forexample, UTRs, terminators, enhancers or promoter variants. Thepromoters upstream of the nucleotide sequences stated can be modified byone or more nucleotide substitution(s), insertion(s) and/or deletion(s)with, however, preferably interfering with the functionality or activityeither of the promoters, the open reading frame (=ORF) or with the3′-regulatory region such as terminators or other 3′regulatory regions,which are far away from the ORF. It is furthermore possible that theactivity of the promoters is decreased by modification of their sequenceor their regulation, or that they are replaced completely by less activepromoters and thereby the activity of the expressed nucleic acidsequence is reduced or deleted, even promoters from heterologousorganisms. Appropriate promoters are known to the person skilled in theart and are mentioned herein be low. Further methods exists to modulatethe promoters of the genes of the invention, e.g. by modifying theactivity of transacting factors, meaning natural or artificialtranscription factors, which can bind to the promoter and influence itsactivity. Furthermore it is possible to influence promoters of interestby modifying upstream signaling components like receptors or kinases,which are involved in the regulation of the promoter of interest.

In a further embodiment, the process according to the present inventioncomprises the following steps:

-   (a) selecting an organism or a part thereof expressing the    polypeptide of this invention;-   (b) mutagenizing the selected organism or the part thereof;-   (c) comparing the activity or the expression level of said    polypeptide in the mutagenized organism or the part thereof with the    activity or the expression of said polypeptide in the selected    organisms or the part thereof;-   (d) selecting the mutagenized organisms or parts thereof, which    comprise a decreased activity or expression level of said    polypeptide compared to the selected organism (a) or the part    thereof;-   (e) optionally, growing and cultivating the organisms or the parts    thereof; and-   (f) recovering, and optionally isolating, the free or bound compound    of the invention produced by the selected mutated organisms or parts    thereof.

The organisms or part thereof produce according to the herein mentionedprocess of the invention an increased level of free and/or protein-boundmethionine compared to said control or selected organisms or partsthereof.

Advantageously the selected organisms were mutagenized according to theinvention. According to the invention mutagenesis is any change of thegenetic information in the genome of an organism, that means anystructural or compositional change in the nucleic acid preferably DNA ofan organism that is not caused by normal segregation or geneticrecombination processes. Such mutations may occur spontaneously, or maybe induced by mutagens as described below. Such change can be inducedeither randomly or selectively. In both cases the genetic information ofthe organism is modified. In general this leads to the situation thatthe activity of the gene product of the relevant genes inside the cellsor inside the organism is reduced or repressed.

In case of the specific or so called site directed mutagenesis adistinct gene is mutated and thereby its activity and/or the activity orthe encoded gene product is repressed, reduced, decreased or deleted. Inthe event of a random mutagenesis one or more genes are mutated bychance and their activities and/or the activities of their gene productsare repressed, reduced, decreased or deleted, preferably decreased ordeleted.

For the purpose of a mutagenesis of a huge population of organisms, suchpopulation can be transformed with a DNA population or a DNA bank, whichare useful for the inhibition of as much as possible genes of anorganism, preferably all genes. With this method it is possible tostatistically mutagenize nearly all genes of an organism by theintegration of an advantageously identified DNA-fragment. Afterwards theskilled worker can easily identify the knocked out event. For themutagenesis of plants EMS, T-DNA and/or transposon mutagenesis ispreferred.

In the event of a random mutagenesis a huge number of organisms aretreated with a mutagenic agent. The amount of said agent and theintensity of the treatment will be chosen in such a manner thatstatistically nearly every gene is mutated. The process for the randommutagenesis as well as the respective agents is well known by theskilled person. Such methods are disclosed for example by A. M. vanHarten [(1998), “Mutation breeding: theory and practical applications”,Cambridge University Press, Cambridge, UK], E Friedberg, G Walker, WSiede [(1995), “DNA Repair and Mutagenesis”, Blackwell Publishing], orK. Sankaranarayanan, J. M. Gentile, L. R. Ferguson [(2000), “Protocolsin Mutagenesis”, Elsevier Health Sciences]. As the skilled worker knowsthe spontaneous mutation rate in the cells of an organism is very lowand that a large number of chemical, physical or biological agents areavailable for the mutagenesis of organisms. These agents are named asmutagens or mutagenic agents. As mentioned before three different kindsof mutagens chemical, physical or biological agents are available.

There are different classes of chemical mutagens, which can be separatedby their mode of action. For example base analogues such as5-bromouracil, 2-amino purin. Other chemical mutagens are interactingwith the DNA such as sulphuric acid, nitrous acid, hydroxylamine; orother alkylating agents such as monofunctional agents like ethylmethanesulfonate (=EMS), dimethylsulfate, methyl methanesulfonate,bifunctional like dichloroethyl sulphide, Mitomycin,Nitrosoguanidine—dialkylnitrosamine, N-Nitrosoguanidin derivatives,N-alkyl-N-nitro-N-nitrosoguanidine, intercalating dyes like Acridine,ethidium bromide.

Physical mutagens are for example ionizing irradiation (X-ray), UVirradiation. Different forms of irradiation are available and they arestrong mutagens. Two main classes of irradiation can be distinguished:a) non-ionizing irradiation such as UV light or ionizing irradiationsuch as X-ray. Biological mutagens are for example transposable elementsfor example IS elements such as IS100, transposons such as Tn5, Tn10,Tn903, Tn916 or Tn1000 or phages like Mu^(amplac), P1, T5, λplac etc.Methods for introducing this phage DNA into the appropriatemicroorganism are well known to the skilled worker (see Microbiology,Third Edition, Eds. Davis, B. D., Dulbecco, R., Eisen, H. N. andGinsberg, H. S., Harper International Edition, 1980). The commonprocedure of a transposon mutagenesis is the insertion of a transposableelement within a gene or nearby for example in the promotor orterminator region and thereby leading to a loss of the gene function.Procedures to localize the transposon within the genome of the organismsare well known by a person skilled in the art. For transposonmutagenesis in plants the maize transposon systemsActivator-Dissociation (Ac/Ds) and Enhancer-Supressor mutator (En/Spm)are known to the worker skilled in the art but other transposon systemsmight be similar useful. The transposons can be brought into the plantgenomes by different available standard techniques for planttransformations. Another type of biological mutagenesis in plantsincludes the T-DNA mutagenesis, meaning the random integration of T-DNAsequences into the plant genome [Feldmann, K. A. (1991) T-DNA insertionmutagenesis in Arabidopsis: Mutational spectrum. Plant J. 1, 71-82]. Theevent in which the gene of interest is mutated can later be searched byPCR- or other high throughput technologies [Krysan et al., (1999) T_DNAas an insertional mutagen in Arabidopsis, Plant Cell, 11, 2283-2290].

Methods for the creation of microorganisms with a reduced decreased ordeleted biological activity of the nucleic acid sequence of theinvention or the encoded proteins are well known to the person skilledin the art. In gram-positive bacteria such as Brevibacterium,Corynebacterium, Rhodococcus or Bacillus IS elements can be used forsaid purpose as described by Schafer et al. [Gene 1994, Jul. 22, 145(1): 69-73]. Methods for the mutagenese of bacteria are well known forthe skilled worker.

Another very efficient method is the introduction of mutations into thegenome of bacteria with the aid of transposons (=Tn). Transposons havesome common properties, which make them useful as tool for themutagenesis. Such properties are for example ubiquitous finding innature for example they are found on chromosomes, plasmids and phages.The transposition of the transposons in the genome is rec-independentand has a general frequency of 10⁻⁴-10⁻⁷. Transposons are in thepossession of an encoded transposase and inverted terminal repeats attheir ends. Furthermore they need for integration in the genome aminimal target sequence specificity, bordering on random. Like plasmidsthey often confer antibiotic resistance. As an advantage they generatepolar mutations. Three kinds of transposons are distinguished from oneanother a) conservative transposons: copy number doesn't increase upontransposition; b) replicative transposons: transposon is copied upontransposition resulting in two copies and c) conjugative transposons:transposon encodes Tra functions, excises and transfers to another host.As the skilled worker knows methods have been developed which facilitatethe introduction of transposable elements into a wide variety of bothgram negative and gram positive bacteria. Therefore transposableelements can be introduced into the genome of nearly every bacteria.They insert somewhat randomly thus causing insertion mutations. Sincethe average bacterial species has approximately 3000 genes, one cansaturate the chromosome with ease. Furthermore, the transposon providesa molecular tag, which can be subsequently used to identify the mutatedgene and clone it. In combination with genomics, transposons are apowerful approach to mutate distinct genes. As mentioned before thereare many different methods to introduce transposons into bacteria. Thechoice will depend on the nature of the target bacterium. Transposonscan be introduced into the genome of a bacteria for example with the aidof a temperature-sensitive replicon, a so called bump plasmid byintroduction of an incompatible replicon, a transfer plasmid lackingessential replication protein supplied in trans in donor cell or phagethat lacks replication in the host organism. Typically well knowntransposons are Tn5, Tn10, Tn903, Tn916, Tn1000 etc.

Other methods are for example the introduction of mutation with the aidof viruses such as bacteriophages such as P1, P22, T2, T3, T5, T7,Mu^(amplac), Mu, Mu1, MuX, miniMu, λ, λplac or insertion elements suchas IS3, IS100, IS900 etc. Again the whole genome of the bacteria israndomly mutagenized. Mutants can be easily identified.

Another method to disrupt the nucleic acid sequence of the invention andthereby reducing, decreasing or deleting the biological activity of theencoded polypeptide can be reached by homologous recombination with analtered nucleic acid sequence of the invention. The nucleic acidsequences of the invention can therefore be altered by one or more pointmutations, deletions, or inversions, but still encodes a functionalprotein of the invention or a non-functional protein. In anotherembodiment of the invention, one or more of the regulatory regions(e.g., a promoter, repressor, or inducer) of the gene encoding theprotein of the invention has been altered (e.g., by deletion,truncation, inversion, or point mutation) such that the expression ofthe corresponding gene is modulated that means reduced, decreased ordeleted.

Preferably a chemical or biochemical procedure is used for themutagenesis of the organisms. A preferred chemical method is themutagenesis with N-methyl-N-nitro-nitrosoguanidine.

Other biological methods are disclosed by Spee et al. (Nucleic AcidsResearch, Vol. 21, No. 3, 1993: 777-778). Spee et al. teaches a PCRmethod using dITP for the random mutagenesis. This method described bySpee et al. was further improved by Rellos et al. (Protein Expr. Purif.,5, 1994: 270-277). The use of an in vitro recombination technique formolecular mutagenesis is described by Stemmer (Proc. Natl. Acad. Sci.USA, Vol. 91, 1994: 10747-10751). Moore et al. (Nature BiotechnologyVol. 14, 1996: 458-467) describe the combination of the PCR andrecombination methods for increasing the enzymatic activity of anesterase toward a para-nitrobenzyl ester. Another route to themutagenesis of enzymes is described by Greener et al. in Methods inMolecular Biology (Vol. 57, 1996: 375-385). Greener et al. use thespecific Escherichia coli strain XL1-Red to generate Escherichia colimutants which have increased antibiotic resistance.

In one embodiment, the protein according to the invention or the nucleicacid molecule characterized herein originates from an eukaryotic orprokaryotic organism such as a non-human animal, a plant, amicroorganism such as a fungi, a yeast, an alga, a diatom or abacterium. Nucleic acids, which advantageously can be used in theinventive process originate from plants, for example the familyBrassicaceae, in particular the genus Brassica, Melanosinapis, Sinapisor Arabidopsis and the especially advantageous from the speciesArabidopsis thaliana. In addition nucleic acid sequences, whichadvantageously can be used in the inventive process originate fromplants such as maize, soja, canola, wheat, barley, triticale, rice,linseed, sunflower or potato. Sequence SEQ ID NO: 2 is deposited underthe Genebank Accession number At2g25320 (RefSeq Accession NM_(—)128089).Sequence SEQ ID NO: 8 is deposited under the Genebank Accession numberAt3g07610 (RefSeq Accession NP_(—)187418). Sequence SEQ ID NO: 20 isdeposited under the Genebank Accession number At3g15600 (RefSeqAccession NM_(—)112428.1). Sequence SEQ ID NO: 65 is deposited under theGenebank Accession number At1g14490 (RefSeq Accession NM_(—)101316).Sequence SEQ ID NO: 152 is deposited under the Genebank Accession numberAt1g30570 (RefSeq Accession NM_(—)102794). Sequence SEQ ID NO: 229 isdeposited under the Genebank Accession number At2g21290 (RefSeqAccession NM_(—)127701) and the sequence SEQ ID NO: 283 is depositedunder the Genebank Accession number At3g14230 (RefSeq AccessionNM_(—)108251).

In another preferred embodiment of the invention the nucleic acids,which can be used in the inventive process originate from the familyEnterobacteriacea such as from the genera Echerichia, Salmonella,Klebsiella as mentioned above for example from the species E. coli.

Accordingly, in one embodiment, the invention relates to an isolatednucleic acid molecule which comprises a nucleic acid molecule selectedfrom the group consisting of:

-   a) nucleic acid molecule which encodes a polypeptide comprising the    polypeptide shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ    ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:    16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ    ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO:    67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ    ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO:    85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ    ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:    103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111,    SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ    ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID    NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO:    137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152,    SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ    ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID    NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO:    178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186,    SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ    ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID    NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO:    212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,    SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID    NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO:    250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258,    SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ    ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID    NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO:    293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301,    SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ    ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID    NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO:    327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335,    SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ    ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID    NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO:    361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369,    SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ    ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID    NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:    395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403,    SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ    ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID    NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO:    429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:    441;-   b) nucleic acid molecule comprising the polynucleotide shown in SEQ    ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10,    SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID    NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30,    SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID    NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79,    SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID    NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,    SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ    ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID    NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO:    123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131,    SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ    ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID    NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO:    164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172,    SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ    ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID    NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO:    198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206,    SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ    ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID    NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO:    239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252,    SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ    ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID    NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:    287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295,    SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ    ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID    NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO:    321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329,    SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ    ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID    NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO:    355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363,    SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ    ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID    NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO:    389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397,    SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ    ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID    NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:    423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,    SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   c) nucleic acid molecule comprising a nucleic acid sequence, which,    as a result of the degeneracy of the genetic code, can be derived    from a polypeptide sequence depicted (b) and having the biological    activity represented by the protein as depicted in SEQ ID NO: 2, SEQ    ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12,    SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID    NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32;    SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID    NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81,    SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID    NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99,    SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ    ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID    NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:    125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,    SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ    ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID    NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO:    166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174,    SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ    ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID    NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:    200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208,    SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ    ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID    NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO:    241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254,    SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ    ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID    NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO:    289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297,    SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ    ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID    NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:    323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331,    SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ    ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID    NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:    357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365,    SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ    ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID    NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO:    391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399,    SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ    ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID    NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO:    425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433,    SEQ ID NO: 435 or SEQ ID NO: 441;-   d) nucleic acid molecule encoding a polypeptide having at least 50%    identity with the amino acid sequence of the polypeptide encoded by    the nucleic acid molecule of (a) or (c) and having the biological    activity represented by the protein as depicted in SEQ ID NO: 2, SEQ    ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12,    SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID    NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32;    SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID    NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81,    SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID    NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99,    SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ    ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID    NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:    125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,    SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ    ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID    NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO:    166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174,    SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ    ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID    NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:    200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208,    SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ    ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID    NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO:    241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254,    SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ    ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID    NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO:    289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297,    SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ    ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID    NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:    323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331,    SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ    ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID    NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:    357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365,    SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ    ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID    NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO:    391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399,    SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ    ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID    NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO:    425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433,    SEQ ID NO: 435 or SEQ ID NO: 441;-   e) nucleic acid molecule which comprises a polynucleotide, which is    obtained by amplifying a cDNA library or a genomic library using the    primers in SEQ ID NO: 58, SEQ ID NO: 59, SEQ ID NO: 60, SEQ ID NO:    61, SEQ ID NO: 62, SEQ ID NO: 63, SEQ ID NO: 144, SEQ ID NO: 145,    SEQ ID NO: 221, SEQ ID NO: 222, SEQ ID NO: 244, SEQ ID NO: 245, SEQ    ID NO: 436 or SEQ ID NO: 437;-   f) nucleic acid molecule encoding a polypeptide, which is isolated    with the aid of monoclonal and/or polyclonal antibodies against a    polypeptide encoded by one of the nucleic acid molecules of (a)    to (c) and having the biological activity represented by the protein    as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:    8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ    ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO:    28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ    ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO:    77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ    ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO:    95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103,    SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ    ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID    NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO:    129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137,    SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ    ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID    NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO:    170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178,    SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ    ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID    NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:    204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,    SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ    ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:    237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250,    SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ    ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID    NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO:    285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293,    SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ    ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID    NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO:    319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327,    SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ    ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID    NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO:    353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361,    SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ    ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID    NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:    387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,    SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ    ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID    NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO:    421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429,    SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   g) nucleic acid molecule encoding a polypeptide comprising the    consensus sequence shown in SEQ ID NO: 33 or SEQ ID NO: 34 and    having the biological activity represented by the protein as    depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8,    SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID    NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,    SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID    NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,    SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID    NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95,    SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID    NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO:    113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,    SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ    ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID    NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO:    154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162,    SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ    ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID    NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO:    188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196,    SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ    ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID    NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:    229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,    SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ    ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID    NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO:    268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285,    SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ    ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID    NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:    311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319,    SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ    ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID    NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO:    345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353,    SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ    ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID    NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO:    379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387,    SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ    ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID    NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:    413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421,    SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ    ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   h) nucleic acid molecule encoding a polypeptide having the    biological activity represented by the protein as depicted in SEQ ID    NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ    ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:    22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ    ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:    71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ    ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO:    89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ    ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID    NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:    115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,    SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ    ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID    NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO:    156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,    SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ    ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID    NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO:    190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198,    SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ    ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID    NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID    NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO:    239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252,    SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ    ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID    NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:    287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295,    SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ    ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID    NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO:    321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329,    SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ    ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID    NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO:    355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363,    SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ    ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID    NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO:    389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397,    SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ    ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID    NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:    423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,    SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;-   i) nucleic acid molecule which is obtainable by screening a suitable    library under stringent hybridisation conditions with a probe    comprising one of the sequences of the nucleic acid molecule of (a)    to (c) or with a fragment of at least 15 nt, preferably 20 nt, 30    nt, 50 nt, 100 nt, 200 nt or 500 nt of the nucleic acid molecule    characterized in (a) to (i) and encoding a polypeptide having the    biological activity represented by the protein as depicted in SEQ ID    NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ    ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO:    22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ    ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:    71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ    ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO:    89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ    ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID    NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO:    115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,    SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ    ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID    NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO:    156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,    SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ    ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID    NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO:    190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198,    SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ    ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID    NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID    NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO:    239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252,    SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ    ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID    NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:    287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295,    SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ    ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID    NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO:    321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329,    SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ    ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID    NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO:    355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363,    SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ    ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID    NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO:    389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397,    SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ    ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID    NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:    423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,    SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441;    or which comprises a sequence which is complementary thereto;    whereby the nucleic acid molecule according to (a) to (j) is at    least in one or more nucleotides different from the sequence    depicted in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7,    SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID    NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27,    SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID    NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76,    SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID    NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94,    SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID    NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO:    112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120,    SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ    ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID    NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:    151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159,    SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ    ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID    NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO:    185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193,    SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ    ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID    NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO:    219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234,    SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ    ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID    NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO:    265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282,    SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ    ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID    NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO:    308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,    SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ    ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID    NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO:    342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350,    SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ    ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID    NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO:    376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,    SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ    ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID    NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO:    410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418,    SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ    ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID    NO: 440 and/or which encodes a protein which differs at least in    one, two, three, four, five, six, seven, eight, nine, ten or more    amino acids from the protein sequences depicted in SEQ ID NO: 2, SEQ    ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12,    SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID    NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32;    SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID    NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81,    SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID    NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99,    SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ    ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID    NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:    125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,    SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ    ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID    NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO:    166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174,    SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ    ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID    NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:    200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208,    SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ    ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID    NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO:    241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254,    SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ    ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID    NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO:    289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297,    SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ    ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID    NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:    323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331,    SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ    ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID    NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:    357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365,    SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ    ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID    NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO:    391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399,    SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ    ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID    NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO:    425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433,    SEQ ID NO: 435 or SEQ ID NO: 441. In another embodiment, the nucleic    acid molecule depicted in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5,    SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO:    15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ    ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO:    66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ    ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO:    84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ    ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO:    102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,    SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ    ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID    NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO:    136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,    SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ    ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID    NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO:    175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,    SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ    ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID    NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO:    209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,    SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ    ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID    NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO:    255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263,    SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ    ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID    NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:    298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306,    SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ    ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID    NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO:    332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340,    SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ    ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID    NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO:    366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374,    SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ    ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID    NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO:    400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408,    SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ    ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID    NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO:    434 or SEQ ID NO: 440 does not consist of the sequence shown in SEQ    ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9,    SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID    NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29,    SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID    NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78,    SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID    NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96,    SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ    ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID    NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO:    122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130,    SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ    ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO:    153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161,    SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ    ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID    NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO:    187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195,    SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ    ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID    NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO:    228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236,    SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ    ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID    NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO:    267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284,    SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ    ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID    NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO:    310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318,    SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ    ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID    NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO:    344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,    SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ    ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID    NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO:    378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386,    SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ    ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID    NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO:    412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420,    SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ    ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO: 440. In a    further embodiment, the nucleic acid molecule of the present    invention is at least 30% identical to the nucleic acid sequence    depicted in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7,    SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID    NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27,    SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID    NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76,    SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID    NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94,    SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID    NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO:    112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120,    SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ    ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID    NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO:    151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159,    SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ    ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID    NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO:    185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193,    SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ    ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID    NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO:    219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234,    SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ    ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID    NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO:    265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282,    SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ    ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID    NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO:    308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,    SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ    ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID    NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO:    342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350,    SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ    ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID    NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO:    376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384,    SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ    ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID    NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO:    410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418,    SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ    ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID    NO: 440 and less than 100%, preferably less than 99.999%, 99.99% or    99.9%, more preferably less than 99%, 985, 97%, 96% or 95% identical    to the sequence shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5,    SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO:    15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ    ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO:    66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ    ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO:    84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ    ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO:    102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,    SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ    ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID    NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO:    136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,    SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ    ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID    NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO:    175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,    SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ    ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID    NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO:    209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,    SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ    ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID    NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO:    255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263,    SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ    ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID    NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:    298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306,    SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ    ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID    NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO:    332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340,    SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ    ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID    NO: 368, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO:    366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374,    SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ    ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID    NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO:    400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408,    SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ    ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID    NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO:    434 or SEQ ID NO: 440.

Preferably, the nucleic acid molecule a iso does not encode apolypeptide as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ IDNO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ IDNO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ IDNO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115,SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ IDNO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ IDNO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ IDNO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ IDNO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ IDNO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ IDNO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ IDNO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 248, SEQ ID NO: 260,SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ IDNO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ IDNO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305,SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ IDNO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ IDNO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ IDNO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ IDNO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ IDNO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ IDNO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ IDNO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441. In another embodiment,the nucleic acid molecule depicted in SEQ ID NO: 1, SEQ ID NO: 3, SEQ IDNO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ IDNO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66,SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO:76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQ IDNO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94, SEQID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO:104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO:122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO:140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ IDNO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163,SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ IDNO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181,SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ IDNO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199,SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ IDNO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ IDNO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242,SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ IDNO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265,SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ IDNO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292,SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ IDNO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310,SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ IDNO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328,SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ IDNO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346,SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ IDNO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364,SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ IDNO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382,SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ IDNO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400,SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ IDNO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418,SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ IDNO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO:440 does not encode a protein of the sequence shown in SEQ ID NO: 2, SEQID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24,SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ IDNO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93,SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO:121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO:164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO:182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO:218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO:266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO:293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO:329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO:365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO:383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO:419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:441. That means the protein sequences depicted in SEQ ID NO: 2, SEQ IDNO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ IDNO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65,SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO:75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ IDNO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103,SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ IDNO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121,SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ IDNO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139,SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ IDNO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164,SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ IDNO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182,SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ IDNO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200,SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ IDNO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218,SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO:235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO:258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO:285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO:303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO:321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO:339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO:357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO:375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO:393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO:411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO:429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441does not consist of the sequence shown in SEQ ID NO: 2, SEQ ID NO: 4,SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO:26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ IDNO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85,SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO:95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ IDNO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113,SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ IDNO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131,SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ IDNO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156,SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ IDNO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174,SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ IDNO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192,SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ IDNO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ IDNO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235,SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ IDNO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258,SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ IDNO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285,SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ IDNO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303,SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ IDNO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321,SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ IDNO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339,SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ IDNO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357,SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ IDNO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375,SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ IDNO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393,SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ IDNO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411,SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ IDNO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429,SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441. In afurther embodiment, the protein of the present invention is at least 30%identical to protein sequence depicted in SEQ ID NO: 2, SEQ ID NO: 4,SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14,SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO:26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ IDNO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85,SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO:95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ IDNO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113,SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ IDNO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131,SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ IDNO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156,SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ IDNO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174,SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ IDNO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192,SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ IDNO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210,SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ IDNO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235,SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ IDNO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258,SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ IDNO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285,SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ IDNO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303,SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ IDNO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321,SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ IDNO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339,SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ IDNO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357,SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ IDNO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375,SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ IDNO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393,SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ IDNO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411,SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ IDNO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429,SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 andless than 100%, preferably less than 99.999%, 99.99% or 99.9%, morepreferably less than 99%, 985, 97%, 96% or 95% identical to the sequenceshown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ IDNO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30,SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO:71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ IDNO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99,SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ IDNO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117,SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ IDNO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135,SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ IDNO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160,SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ IDNO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178,SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ IDNO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196,SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ IDNO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214,SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ IDNO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239,SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ IDNO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262,SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ IDNO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289,SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ IDNO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307,SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ IDNO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325,SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ IDNO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343,SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ IDNO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361,SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ IDNO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379,SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ IDNO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397,SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ IDNO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415,SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ IDNO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433,SEQ ID NO: 435 or SEQ ID NO: 441.

The nucleic acid sequences used in the process are advantageouslyintroduced in a nucleic acid construct, preferably an expressioncassette, which allows the reduction, depression etc. of the nucleicacid molecules in an organism, advantageously a plant or amicroorganism.

Accordingly, the invention also relates to a nucleic acid construct,preferably to an expression construct, comprising the nucleic acidmolecule of the present invention or a fragment thereof functionallylinked to one or more regulatory elements or signals.

As described herein, the nucleic acid construct can also comprisefurther genes, which are to be introduced into the organisms or cells.It is possible and advantageous to introduce into, and express in, thehost organisms regulatory genes such as genes for inductors, repressorsor enzymes, which, owing to their enzymatic activity, engage in theregulation of one or more genes of a biosynthetic pathway. These genescan be of heterologous or homologous origin. Moreover, furtherbiosynthesis genes may advantageously be present, or else these genesmay be located on one or more further nucleic acid constructs. Genes,which are advantageously employed as biosynthesis genes are genes of theamino acid metabolism, of glycolysis, of the tricarboxylic acidmetabolism or their combinations.

As described herein, regulator sequences or factors can have a positiveeffect on preferably the gene expression of the genes introduced, thusincreasing it. Thus, an enhancement of the regulator elements mayadvantageously take place at the transcriptional level by using strongtranscription signals such as promoters and/or enhancers. In addition,however, an enhancement of translation is also possible, for example byincreasing RNA stability. On the other hand the nucleic acid moleculesof the invention and the gene products are reduced, decreased or deletedto increase the productivity of the fine chemical.

In principle, the nucleic acid construct can comprise the hereindescribed regulator sequences and further sequences relevant for thereduction of the expression of nucleic acid molecules of the inventionand on the other side for the expression of additional genes in theconstruct. Thus, the nucleic acid construct of the invention can be usedas expression cassette and thus can be used directly for introductioninto the plant, or else they may be introduced into a vector.Accordingly in one embodiment the nucleic acid construct is anexpression cassette comprising a microorganism promoter or amicroorganism terminator or both. In another embodiment the expressioncassette encompasses a plant promoter or a plant terminator or both.

Accordingly, in one embodiment, the process according to the inventioncomprises the following steps:

-   (a) introduction of a nucleic acid construct comprising the nucleic    acid molecule of the invention, which encodes the polypeptide of the    present invention; or-   (b) introduction of a nucleic acid molecule, including regulatory    sequences or factors, which expression decreases the expression of    the polypeptide of the invention;    in a cell, or an organism or a part thereof, preferably in a plant,    plant cell or a microorganism, and-   (c) repressing the polypeptide encoded by the nucleic acids of the    invention by the nucleic acid construct or the nucleic acid molecule    mentioned under (a) or (b) in the cell or the organism.

After the introduction and expression of the nucleic acid construct thetransgenic organism or cell is advantageously cultured and subsequentlyharvested. The transgenic organism or cell may be a prokaryotic oreukaryotic organism such as a microorganism, a non-human animal andplant for example a plant or animal cell, a plant or animal tissue,preferably a crop plant, or a part thereof.

To introduce a nucleic acid molecule for example an RNAi, antisensenucleic acid sequence or a mutagenized nucleic acid sequence into anucleic acid construct, e.g. as part of an expression cassette, whichleads to a reduced activity and/or expression of the respective gene,the codogenic gene segment or the untranslated regions areasadvantageously subjected to an amplification and ligation reaction inthe manner known by a skilled person. It is preferred to follow aprocedure similar to the protocol for the Pfu DNA polymerase or aPfu/Taq DNA polymerase mixture. The primers are selected according tothe sequence to be amplified. The primers should expediently be chosenin such a manner that the amplificate comprise at least the codogenicsequence from the start to the stop codon. Additional possibilitiesinclude the 5′ or 3′ untranslated regions or the promoter region. Afterthe amplification, the amplificate is expediently analyzed. For example,the analysis may consider quality and quantity and be carried outfollowing separation by gel electrophoresis. Thereafter, the amplificatecan be purified following a standard protocol (for example Qiagen). Analiquot of the purified amplificate is then available for the subsequentcloning step. Suitable cloning vectors are generally known to theskilled worker [Cloning Vectors (Eds. Pouwels P. H. et al. Elsevier,Amsterdam-New York-Oxford, 1985, ISBN 0 444 904018)].

They include, in particular, vectors which are capable of replication ineasy to handle cloning systems like bacterial yeast or insect cell based(e.g. baculovirus expression) systems, that is to say especially vectorswhich ensure efficient cloning in E. coli, and which make possible thestable transformation of plants. Vectors, which must be mentioned, inparticular are various binary and cointegrated vector systems, which aresuitable for the T-DNA-mediated transformation. Such vector systems aregenerally characterized in that they contain at least the vir genes,which are required for the Agrobacterium-mediated transformation, andthe T-DNA border sequences.

In general, vector systems preferably also comprise furthercis-regulatory regions such as promoters and terminators and/orselection markers by means of which suitably transformed organisms canbe identified. While vir genes and T-DNA sequences are located on thesame vector in the case of cointegrated vector systems, binary systemsare based on at least two vectors, one of which bears vir genes, but noT-DNA, while a second one bears T-DNA, but no vir gene. Owing to thisfact, the last-mentioned vectors are relatively small, easy tomanipulate and capable of replication in E. coli and in Agrobacterium.These binary vectors include vectors from the series pBIB-HYG, pPZP,pBecks, pGreen. Those, which are preferably used in accordance with theinvention, are Bin19, pBI101, pBinAR, pSun, pGPTV and pCAMBIA. Anoverview of binary vectors and their use is given by Hellens et al,Trends in Plant Science (2000) 5, 446-451.

For a vector preparation, vectors may first be linearized usingrestriction endonuclease(s) and then be modified enzymatically in asuitable manner. Thereafter, the vector is purified, and an aliquot isemployed in the cloning step. In the cloning step, the enzyme-cleavedand, if required, purified amplificate is cloned together with similarlyprepared vector fragments, using ligase. In this context, a specificnucleic acid construct, or vector or plasmid construct, may have one orelse more codogenic gene segments. The codogenic gene segments in theseconstructs are preferably linked operably to regulatory sequences. Theregulatory sequences include, in particular, plant sequences like theabove-described promoters and terminators. The constructs canadvantageously be propagated stably in microorganisms, in particularEscherichia coli and/or Agrobacterium tumefaciens, under selectiveconditions and enable the transfer of heterologous DNA into plants orother microorganisms. In accordance with a particular embodiment, theconstructs are based on binary vectors (overview of a binary vector:Hellens et al., 2000). As a rule, they contain prokaryotic regulatorysequences, such as replication origin and selection markers, for themultiplication in microorganisms such as Escherichia coli andAgrobacterium tumefaciens. Vectors can further contain agrobacterialT-DNA sequences for the transfer of DNA into plant genomes or othereukaryotic regulatory sequences for transfer into other eukaryoticcells, e.g. Saccharomyces sp. or other prokaryotic regulatory sequencesfor the transfer into other prokaryotic cells, e.g. Corynebacterium sp.or Bacillus sp. For the transformation of plants, at least the rightborder sequence, which comprises approximately 25 base pairs, of thetotal agrobacterial T-DNA sequence is required. Usually, the planttransformation vector constructs according to the invention containT-DNA sequences both from the right and from the left border region,which contain expedient recognition sites for site-specific actingenzymes, which, in turn, are encoded by some of the vir genes.

Suitable host organisms are known to the skilled worker. Advantageousorganisms are described further above in the present application. Theyinclude in particular eukaryotes or eubacteria, e.g. prokaryotes orarchae bacteria. Advantageously host organisms are microorganismsselected from the group consisting of Actinomycetaceae, Bacillaceae,Brevibacteriaceae, Corynebacteriaceae, Enterobacteriacae, Gordoniaceae,Micrococcaceae, Mycobacteriaceae, Nocardiaceae, Pseudomonaceae,Rhizobiaceae, Streptomycetaceae, Chaetomiaceae, Choanephoraceae,Cryptococcaceae, Cunninghamellaceae, Demetiaceae, Moniliaceae,Mortierellaceae, Mucoraceae, Pythiaceae, Sacharomycetaceae,Saprolegniaceae, Schizosacharomycetaceae, Sodariaceae,Sporobolomycetaceae, Tuberculariaceae, Adelotheciaceae, Dinophyceae,Ditrichaceae and Prasinophyceae. Preferably are unicellularmicroorganisms, e.g. fungi, bacteria or protoza, such as fungi like thegenus Claviceps or Aspergillus or gram-positive bacteria such as thegenera Bacillus, Corynebacterium, Micrococcus, Brevibacterium,Rhodococcus, Nocardia, Caseobacter or Arthrobacter or gram-negativebacteria such as the genera Escherichia, Flavobacterium or Salmonella,or yeasts such as the genera Rhodotorula, Hansenula, Pichia, Yerrowia,Saccharomyces, Schizosaccharomyces or Candida.

Host organisms which are especially advantageously selected in theprocess according to the invention are microorganisms selected from thegroup of the genera and species consisting of Hansenula anomala, Candidautilis, Claviceps purpurea, Bacillus circulans, Bacillus subtilis,Bacillus sp., Brevibacterium albidum, Brevibacterium album,Brevibacterium cerinum, Brevibacterium flavum, Brevibacteriumglutamigenes, Brevibacterium iodinum, Brevibacterium ketoglutamicum,Brevibacterium lactofermentum, Brevibacterium linens, Brevibacteriumroseum, Brevibacterium saccharolyticum, Brevibacterium sp.,Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum,Corynebacterium ammoniagenes, Corynebacterium glutamicum (=Micrococcusglutamicum), Corynebacterium melassecola, Corynebacterium sp. orEscherichia coli, specifically Escherichia coli K12 and its describedstrains.

Advantageously preferred in accordance with the invention are hostorganisms of the genus Agrobacterium tumefaciens or plants. Preferredplants are selected from among the families Aceraceae, Anacardiaceae,Apiaceae, Asteraceae, Apiaceae, Betulaceae, Boraginaceae, Brassicaceae,Bromeliaceae, Cactaceae, Caricaceae, Caryophyllaceae, Cannabaceae,Convolvulaceae, Chenopodiaceae, Elaeagnaceae, Geraniaceae, Gramineae,Juglandaceae, Lauraceae, Leguminosae, Linaceae, Cucurbitaceae,Cyperaceae, Euphorbiaceae, Fabaceae, Malvaceae, Nymphaeaceae,Papaveraceae, Rosaceae, Salicaceae, Solanaceae, Arecaceae, Iridaceae,Liliaceae, Orchidaceae, Gentianaceae, Labiaceae, Magnoliaceae,Ranunculaceae, Carifolaceae, Rubiaceae, Scrophulariaceae, Ericaceae,Polygonaceae, Violaceae, Juncaceae, Poaceae, perennial grass, foddercrops, vegetables and ornamentals.

Especially preferred are plants selected from the groups of the familiesApiaceae, Asteraceae, Brassicaceae, Cucurbitaceae, Fabaceae,Papaveraceae, Rosaceae, Solanaceae, Liliaceae or Poaceae. Especiallyadvantageous are, in particular, crop plants. Accordingly, anadvantageous plant preferably belongs to the group of the genus peanut,oilseed rape, canola, sunflower, safflower, olive, sesame, hazelnut,almond, avocado, bay, pumpkin/squash, linseed, soya, pistachio, borage,maize, wheat, rye, oats, sorghum and millet, triticale, rice, barley,cassava, potato, sugarbeet, fodder beet, egg plant, and perennialgrasses and forage plants, oil palm, vegetables (brassicas, rootvegetables, tuber vegetables, pod vegetables, fruiting vegetables, onionvegetables, leafy vegetables and stern vegetables), buckwheat, Jerusalemartichoke, broad bean, vetches, lentil, alfalfa, dwarf bean, lupin,clover and lucerne. Further preferred plants are mentioned above.

In order to introduce, into a plant, the nucleic acid molecule of theinvention or used in the process according to the invention for examplean RNAi, antisense nucleic acid sequence or a mutagenized nucleic acidsequence, it has proved advantageous first to transfer them into anintermediate host, for example a bacterium or a eukaryotic unicellularcell. The transformation into E. coli, which can be carried out in amanner known per se, for example by means of heat shock orelectroporation, has proved itself expedient in this context. Thus, thetransformed E. coli colonies can be analysed for their cloningefficiency. This can be carried out with the aid of a PCR. Here, notonly the identity, but also the integrity, of the plasmid construct canbe verified with the aid of a defined colony number by subjecting analiquot of the colonies to said PCR. As a rule, universal primers whichare derived from vector sequences are used for this purpose, it beingpossible, for example, for a forward primer to be arranged upstream ofthe start ATG and a reverse primer to be arranged downstream of the stopcodon of the codogenic gene segment. The amplificates are separated byelectrophoresis and assessed with regard to quantity and quality.

The nucleic acid constructs, which are optionally verified, aresubsequently used for the transformation of the plants or other hosts,e.g. other eukaryotic cells or other prokaryotic cells. To this end, itmay first be necessary to obtain the constructs from the intermediatehost. For example, the constructs may be obtained as plasmids frombacterial hosts by a method similar to conventional plasmid isolation.

Gene silencing in plants can advantageously achieved by transienttransformation technologies, meaning that the nucleic acids arepreferably not integrated into the plant genome. Suitable systems fortransient plant transformations are for example agrobacterium based andplant virus based systems. Details about virus based transient systemsand their use for gene silencing in plants have been described in Lu etal. in Methods 2003, 30(4) 296-303. The use of agrobacterium for thetransient expression of nucleic acids in plants have been described forexample by Fuentes et al., 2003 in Biotechnol Appl Biochem. 2003 Nov. 21online: doi:10.1042/BA20030192).

A large number of methods for the transformation of plants are known.Since, in accordance with the invention, a stable integration ofheterologous DNA into the genome of plants is advantageous, theT-DNA-mediated transformation has proved expedient in particular. Forthis purpose, it is first necessary to transform suitable vehicles, inparticular agrobacteria, with a gene segment or the correspondingplasmid construct comprising the nucleic acid molecule of the invention.This can be carried out in a manner known per se. For example, saidnucleic acid construct of the invention, or said expression construct orsaid plasmid construct, which has been generated in accordance with whathas been detailed above, can be transformed into competent agrobacteriaby means of electroporation or heat shock. In principle, one mustdifferentiate between the formation of cointegrated vectors on the onehand and the transformation with binary vectors on the other hand. Inthe case of the first alternative, the constructs, which comprise thecodogenic gene segment or the nucleic acid molecule of the inventionhave no T-DNA sequences, but the formation of the cointegrated vectorsor constructs takes place in the agrobacteria by homologousrecombination of the construct with T-DNA. The T-DNA is present in theagrobacteria in the form of Ti or Ri plasmids in which exogenous DNA hasexpediently replaced the oncogenes. If binary vectors are used, they canbe transferred to agrobacteria either by bacterial conjugation or bydirect transfer. These agrobacteria expediently already comprise thevector bearing the vir genes (currently referred to as helper Ti(Ri)plasmid).

In addition the stable transformation of plastids is of advantageousbecause plastids are inherited maternally in most crops reducing oreliminating the risk of transgene flow through pollen. The process ofthe transformation of the chloroplast genome is generally achieved by aprocess which has been schematically displayed in Klaus et al., 2004,Nature Biotechnology 22(2), 225-229).

Briefly the sequences to be transformed are cloned together with aselectable marker gene between flanking sequences homologous to thechloroplast genome. These homologous flanking sequences direct sitespecific intergration into the plastome. Plastidal transformation hasbeen described for many different plant species and an overview can betaken from Bock et al. [(2001) Transgenic plastids in basic research andplant biotechnology. J Mol Biol. 2001 Sep. 21; 312(3):425-38] or Maliga,P [Progress towards commercialization of plastid transformationtechnology. Trends Biotechnol. 21, 20-28 (2003)]. Furtherbiotechnological progress has recently been reported in form of markerfree plastid transformants, which can be produced by a transientcointegrated maker gene [Klaus et al., 2004, Nature Biotechnology 22(2),225-229].

One or more markers may expediently also be used together with thenucleic acid construct, or the vector of the invention and, if plants orplant cells shall be transformed together with the T-DNA, with the aidof which the isolation or selection of transformed organisms, such asagrobacteria or transformed plant cells, is possible. These marker genesenable the identification of a successful transfer of the nucleic acidmolecules according to the invention via a series of differentprinciples, for example via visual identification with the aid offluorescence, luminescence or in the wavelength range of light which isdiscernible for the human eye, by a resistance to herbicides orantibiotics, via what are known as nutritive markers (auxotrophismmarkers) or antinutritive markers, via enzyme assays or viaphytohormones. Examples of such markers which may be mentioned are GFP(=green fluorescent protein); the luciferin/luceferase system, theβ-galactosidase with its colored substrates, for example X-Gal, theherbicide resistances to, for example, imidazolinone, glyphosate,phosphinothricin or sulfonylurea, the antibiotic resistances to, forexample, bleomycin, hygromycin, streptomycin, kanamycin, tetracyclin,chloramphenicol, ampicillin, gentamycin, geneticin (G418), spectinomycinor blasticidin, to mention only a few, nutritive markers such as theutilization of mannose or xylose, or antinutritive markers such as theresistance to 2-deoxyglucose. This list is a small number of possiblemarkers. The skilled worker is very familiar with such markers.Different markers are preferred, depending on the organism and theselection method.

As a rule, it is desired that the plant nucleic acid constructs beflanked by T-DNA at one or both sides of the gene segment. This isparticularly useful when bacteria of the species Agrobacteriumtumefaciens or Agrobacterium rhizogenes are used for the transformation.A method, which is preferred in accordance with the invention, is thetransformation with the aid of Agrobacterium tumefaciens. However,biolistic methods may also be used advantageously for introducing thesequences in the process according to the invention, and theintroduction by means of PEG is also possible. The transformedagrobacteria can be grown in the manner known per se and are thusavailable for the expedient transformation of the plants. The plants orplant parts to be transformed are grown or provided in the customarymanner. The transformed agrobacteria are subsequently allowed to act onthe plants or plant parts until a sufficient transformation rate isreached. Allowing the agrobacteria to act on the plants or plant partscan take different forms. For example, a culture of morphogenic plantcells or tissue may be used. After the T-DNA transfer, the bacteria are,as a rule, eliminated by antibiotics, and the regeneration of planttissue is induced. This is done in particular using suitable planthormones in order to initially induce callus formation and then topromote shoot development.

The transfer of foreign genes into the genome of a plant is calledtransformation. In doing this the methods described for thetransformation and regeneration of plants from plant tissues or plantcells are utilized for transient or stable transformation. Anadvantageous transformation method is the transformation in planta. Tothis end, it is possible, for example, to allow the agrobacteria to acton plant seeds or to inoculate the plant meristem with agrobacteria. Ithas proved particularly expedient in accordance with the invention toallow a suspension of transformed agrobacteria to act on the intactplant or at least the flower primordia. The plant is subsequently grownon until the seeds of the treated plant are obtained (Clough and Bent,Plant J. (1998) 16, 735-743). To select transformed plants, the plantmaterial obtained in the transformation is, as a rule, subjected toselective conditions so that transformed plants can be distinguishedfrom untransformed plants. For example, the seeds obtained in theabove-described manner can be planted and, after an initial growingperiod, subjected to a suitable selection by spraying. A furtherpossibility consists in growing the seeds, if appropriate aftersterilization, on agar plates using a suitable selection agent so thatonly the transformed seeds can grow into plants. Further advantageoustransformation methods, in particular for plants, are known to theskilled worker and are described hereinbelow.

A further advantageously suitable methods are protoplast transformationby poly(ethylene glycol)—induced DNA uptake, the “biolistic” methodusing the gene cannon—referred to as the particle bombardment method,electroporation, the incubation of dry embryos in DNA solution,microinjection and gene transfer mediated by Agrobacterium. Said methodsare described by way of example in B. Jenes et al., Techniques for GeneTransfer, in: Transgenic Plants, Vol. 1, Engineering and Utilization,eds. S. D. Kung and R. Wu, Academic Press (1993) 128-143 and in PotrykusAnnu. Rev. Plant Physiol. Plant Molec. Biol. 42 (1991) 205-225). Thenucleic acids or the construct to be expressed is preferably cloned intoa vector, which is suitable for transforming Agrobacterium tumefaciens,for example pBin19 (Bevan et al., Nucl. Acids Res. 12 (1984) 8711).Agrobacteria transformed by such a vector can then be used in knownmanner for the transformation of plants, in particular of crop plantssuch as by way of example tobacco plants, for example by bathing bruisedleaves or chopped leaves in an agrobacterial solution and then culturingthem in suitable media. The transformation of plants by means ofAgrobacterium tumefaciens is described, for example, by Höfgen andWillmitzer in Nucl. Acid Res. (1988) 16, 9877 or is known inter aliafrom F. F. White, Vectors for Gene Transfer in Higher Plants; inTransgenic Plants, Vol. 1, Engineering and Utilization, eds. S. D. Kungand R. Wu, Academic Press, 1993, pp. 15-38.

The abovementioned nucleic acid molecules can be cloned into the nucleicacid constructs or vectors according to the invention in combinationtogether with further genes, or else different genes are introduced bytransforming several nucleic acid constructs or vectors (includingplasmids) into a host cell, advantageously into a plant cell or amicroorganisms.

In addition to the sequence mentioned in SEQ ID NO: 1, SEQ ID NO: 3, SEQID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25,SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO:66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ IDNO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84, SEQID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID NO: 94,SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO: 102, SEQ ID NO:104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110, SEQ ID NO: 112, SEQID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ ID NO: 120, SEQ ID NO:122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128, SEQ ID NO: 130, SEQID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ ID NO: 138, SEQ ID NO:140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151, SEQ ID NO: 153, SEQ IDNO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163,SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ IDNO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181,SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ IDNO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199,SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ IDNO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ IDNO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID NO: 242,SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO: 255, SEQ IDNO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263, SEQ ID NO: 265,SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ ID NO: 282, SEQ IDNO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID NO: 290, SEQ ID NO: 292,SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298, SEQ ID NO: 300, SEQ IDNO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ ID NO: 308, SEQ ID NO: 310,SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316, SEQ ID NO: 318, SEQ IDNO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ ID NO: 326, SEQ ID NO: 328,SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ IDNO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346,SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ IDNO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364,SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ IDNO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382,SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ IDNO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO: 400,SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408, SEQ IDNO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ ID NO: 418,SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID NO: 426, SEQ IDNO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO: 434 or SEQ ID NO:440 or its derivatives, it is advantageous additionally to expressand/or mutate further genes in the organisms. Especially advantageously,additionally at least one further gene of the amino acid biosyntheticpathway such as for L-lysine, L-threonine and/or L-methionine isexpressed in the organisms such as plants or microorganisms. It is alsopossible that the regulation of the natural genes has been modifiedadvantageously so that the gene and/or its gene product is no longersubject to the regulatory mechanisms which exist in the organisms. Thisleads to an increased synthesis of the amino acids desired since, forexample, feedback regulations no longer exist to the same extent or notat all. In addition it might be advantageously to combine the sequencesSEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9,SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO:21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ IDNO: 31; SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQID NO: 72, SEQ ID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80,SEQ ID NO: 82, SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO:90, SEQ ID NO: 92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ IDNO: 100, SEQ ID NO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108,SEQ ID NO: 110, SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ IDNO: 118, SEQ ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126,SEQ ID NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ IDNO: 136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ IDNO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167,SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ IDNO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185,SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ IDNO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203,SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ IDNO: 213, SEQ ID NO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228,SEQ ID NO: 230, SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ IDNO: 238, SEQ ID NO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251,SEQ ID NO: 253, SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ IDNO: 261, SEQ ID NO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269,SEQ ID NO: 271, SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ IDNO: 288, SEQ ID NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296,SEQ ID NO: 298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ IDNO: 306, SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314,SEQ ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ IDNO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332,SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ IDNO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350,SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ IDNO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368,SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ IDNO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386,SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ IDNO: 396, SEQ ID NO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404,SEQ ID NO: 406, SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ IDNO: 414, SEQ ID NO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422,SEQ ID NO: 424, SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ IDNO: 432, SEQ ID NO: 434 or SEQ ID NO: 440, which are reduced in thebiological activity, with genes which generally support or enhance thegrowth or yield of the target organism, for example genes which lead tofaster a growth rate of the microorganisms or genes which for exampleproduces stress-, pathogen, or herbicide resistant plants.

In a further embodiment of the process of the invention, therefore,organisms are grown, in which there is simultaneous overexpression of atleast one nucleic acid or one of the genes which code for proteinsselected from the group of gene products consisting of aspartate kinase(lysC), of aspartate-semialdehyde dehydrogenase (asd), ofglyceraldehyde-3-phosphate dehydrogenase (gap), of 3-phosphoglyceratekinase (pgk), of pyruvate carboxylase (pyc), of triosephosphateisomerase (tpi), of homoserine O-acetyltransferase (metA) , ofcystathionine γ-synthase (metB), of cystathionine gamma-lyase (metC),cystathionine β-lyase, of methionine synthase (metH), of serinehydroxymethyltransferase (glyA), of O-acetylhomoserine sulfhydrylase(metY), of methylenetetrahydrofolate reductase (metF), of phosphoserineaminotransferase (serC), of phosphoserine phosphatase (serB), of serineacetyltransferase (cysE), of cysteine synthase (cysK), of homoserinedehydrogenase (hom) and S-adenosylmethionine synthase (metX).

A further advantageous nucleic acid sequence, which can be expressed incombination with the sequences used in the process and/or theabovementioned biosynthesis genes is the sequence of the ATP/ADPtranslocator as described in WO 01/20009. This ATP/ADP translocatorleads to an increased synthesis of the essential amino acids lysineand/or methionine.

In a further advantageous embodiment of the process of the invention,the organisms used in the process are those in which simultaneously atleast one of the aforementioned genes or one of the aforementionednucleic acids is mutated so that the activity of the correspondingproteins is influenced by metabolites to a smaller extent compared withthe unmutated proteins, or not at all, and that in particular theproduction according to the invention of the amino acids is notimpaired, or so that their specific enzymatic activity is increased.This increased activity of the genes, which are expressed in addition tothe reduced, decreased or deleted activities of the nucleic acidsequences of the invention, leads to an increased production of the finechemical. Less influence means in this connection that the regulation ofthe enzymic activity is less by at least 10%, advantageously at least20, 30 or 40%, particularly advantageously by at least 50, 60 or 70%,compared with the starting organism, and thus the activity of the enzymeis increased by these figures mentioned compared with the startingorganism. An increase in the enzymatic activity means an enzymaticactivity which is increased by at least 10%, advantageously at least 20,30 or 40%, particularly advantageously by at least 50, 60 or 70%,compared with the starting organism. This leads to an increasedproductivity of the fine chemical.

In a further advantageous embodiment of the process of the invention,the organisms used in the process are those in which simultaneously atleast one of the genes selected from homoserine kinase (thrB), threoninedehydratase (ilvA), threonine synthase (thrC), meso-diaminopimelateD-dehydrogenase (ddh), phosphoenolpyruvate carboxykinase (pck),glucose-6-phosphate 6-isomerase (pgi), pyruvate oxidase (poxB),dihydrodipicolinate synthase (dapA), dihydrodipicolinate reductase(dapB) and diaminopicolinate decarboxylase (lysA) or a threonindegrading protein is attenuated, in particular by reducing the rate ofexpression of the corresponding gene.

In another embodiment of the process of the invention, the organismsused in the process are those in which simultaneously at least one ofthe aforementioned nucleic acids or of the aforementioned genes ismutated in such a way that the enzymatic activity of the correspondingprotein is partially reduced or completely blocked. A reduction in theenzymatic activity means an enzymatic activity, which is reduced by atleast 10%, advantageously at least 20, 30 or 40%, particularlyadvantageously by at least 50, 60 or 70%, preferably more, compared withthe starting organism.

If it is intended to transform the host cell, in particular the plantcell, with several constructs or vectors, the marker of a precedingtransformation must be removed or a further marker employed in afollowing transformation. The markers can be removed from the host cell,in particular the plant cell, as described hereinbelow via methods withwhich the skilled worker is familiar. In particular plants without amarker, in particular without resistance to antibiotics, are anespecially preferred embodiment of the present invention.

In the process according to the invention, the nucleic acid sequencesused in the process according to the invention are advantageously linkedoperably to one or more regulatory signals in order to increase geneexpression for example if RNAi or antisense is used. These regulatorysequences are intended to enable the specific expression of the genes orgene fragments. Depending on the host organism for example plant ormicroorganism, this may mean, for example, that the gene or genefragment is expressed and/or overexpressed after induction only, or thatit is expressed and/or overexpressed constitutive. These regulatorysequences are, for example, sequences to which the inductors orrepressors bind and which thus regulate the expression of the nucleicacid. In addition to these novel regulatory sequences, or instead ofthese sequences, the natural regulation of these sequences may still bepresent before the actual structural genes and, if appropriate, may havebeen genetically modified so that the natural regulation has beenswitched off and gene expression has been increased. However, thenucleic acid construct of the invention suitable as expression cassette(=expression construct=gene construct) can also be simpler inconstruction, that is to say no additional regulatory signals have beeninserted before the nucleic acid sequence or its derivatives, and thenatural promoter together with its regulation has not been removed.Instead, the natural regulatory sequence has been mutated in such a waythat regulation no longer takes place and/or gene expression isincreased. These modified promoters can also be introduced on their ownbefore the natural gene in the form of part sequences (=promoter withparts of the nucleic acid sequences according to the invention) in orderto increase the activity. Moreover, the gene construct canadvantageously also comprise one or more of what are known as enhancersequences in operable linkage with the promoter, and these enable anincreased expression of the nucleic acid sequence. Also, it is possibleto insert additional advantageous sequences at the 3′ end of the DNAsequences, such as, for example, further regulatory elements orterminators.

The nucleic acid molecules, which encode proteins according to theinvention and nucleic acid molecules, which encode other polypeptidesmay be present in one nucleic acid construct or vector or in severalones. Advantageously, only one copy of the nucleic acid molecule of theinvention or its encoding genes is present in the nucleic acid constructor vector. Several vectors or nucleic acid construct or vector can beexpressed together in the host organism. The nucleic acid molecule orthe nucleic acid construct or vector according to the invention can beinserted in a vector and be present in the cell in a free form. If astable transformation is preferred, a vector is used, which is stablyduplicated over several generations or which is else be inserted intothe genome. In the case of plants, integration into the plastid genomeor, in particular, into the nuclear genome may have taken place. For theinsertion of more than one gene in the host genome the genes to beexpressed are present together in one gene construct, for example inabove-described vectors bearing a plurality of genes.

As a rule, regulatory sequences for the expression rate of a gene arelocated upstream (5′), within, and/or downstream (3′) relative to thecoding sequence of the nucleic acid molecule of the invention or anothercodogenic gene segment. They control in particular transcription and/ortranslation and/or the transcript stability. The expression level isdependent on the conjunction of further cellular regulatory systems,such as the protein biosynthesis and degradation systems of the cell.

Regulatory sequences include transcription and translation regulatingsequences or signals, e.g. sequences located upstream (5′), whichconcern in particular the regulation of transcription or translationinitiation, such as promoters or start codons, and sequences locateddownstream (3′), which concern in particular the regulation oftranscription or translation termination and transcript stability, suchas polyadenylation signals or stop codons. Regulatory sequences can alsobe present in transcribed coding regions as well in transcribednon-coding regions, e.g. in introns, as for example splicing sites.

Promoters for the regulation of expression of the nucleic acid moleculeaccording to the invention in a cell and which can be employed are, inprinciple, all those which are capable of reducing the transcription ofthe nucleic acid molecules or stimulating the transcription ofadditional genes in the organisms in question, such as microorganisms orplants, depending on the goal, which should be reached by using saidpromoters. Suitable promoters, which are functional in these organisms,are generally known. They may take the form of constitutive or induciblepromoters. Suitable promoters can enable the development- and/ortissue-specific expression in multi-celled eukaryotes; thus, leaf-,root-, flower-, seed-, stomata-, tuber- or fruit-specific promoters mayadvantageously be used in plants.

Advantageous regulatory sequences for the expression of the nucleic acidmolecule according to the invention in microorganisms are present forexample in promoters such as the cos, tac, rha, trp, tet, trp-tet, lpp,lac, lpp-lac, lacI^(q−), T7, T5, T3, gal, trc, ara, SP6, λ-P_(R) orλ-P_(L) promoter, which are advantageously used in gram-negativebacteria. Further advantageous regulatory sequences are present forexample in the gram-positive promoters amy, dnaK, xylS and SPO2, in theyeast or fungal promoters ADC1, MFα, AC, P-60, UASH, MCB, PHO, CYC1,GAPDH, TEF, rp28, ADH. Promoters, which are particularly advantageous,are constitutive, tissue or compartment specific and induciblepromoters. In general, “promoter” is understood as meaning, in thepresent context, a regulatory sequence in a nucleic acid molecule, whichmediates the expression of a coding sequence segment of a nucleic acidmolecule. In general, the promoter is located upstream to the codingsequence segment. Some elements, for example expression-enhancingelements such as enhancer may, however, also be located downstream oreven in the transcribed region.

In principle, it is possible to use natural promoters together withtheir regulatory sequences, such as those mentioned above, for the novelprocess. It is also possible advantageously to use synthetic promoters,either additionally or alone, in particular when they mediateseed-specific expression such as described in, for example, WO 99/16890.

The expression of the nucleic acid molecules used in the process may bedesired alone or in combination with other genes or nucleic acids.Multiple nucleic acid molecules conferring repression or expression ofadvantageous genes, depending on the goal to be reached, can beintroduced via the simultaneous transformation of several individualsuitable nucleic acid constructs, i.e. expression constructs, or,preferably, by combining several expression cassettes on one construct.It is also possible to transform several vectors with in each caseseveral expression cassettes stepwise into the recipient organism.

As described above, the transcription of the genes, which are inaddition to the genes of the invention introduced should advantageouslybe terminated by suitable terminators at the 3′ end of the biosynthesisgenes introduced (behind the stop codon). A terminator, which may beused for this purpose is, for example, the OCS1 terminator, the nos3terminator or the 35S terminator. As is the case with the promoters,different terminator sequences should be used for each gene.Terminators, which are useful in microorganism are for example the fimAterminator, txn terminator or trp terminator. Such terminators can berho-dependent or rho-independent.

Different plant promoters such as, for example, the USP, the LegB4-, theDC3 promoter or the ubiquitin promoter from parsley or other hereinmentioned promoter and different terminators may advantageously be usedin the nucleic acid construct. Further useful plant promoters are forexample the maize ubiquitin promoter, the ScBV (Sugarcaine bacilliformvirus) promoter, the lpt2 or lpt1-gene promoters from barley (WO95/15389 and WO 95/23230) or those described in WO 99/16890 (promotersfrom the barley hordein-gene, the rice glutelin gene, the rice oryzingene, the rice prolamin gene, the wheat gliadin gene, wheat glutelingene, the maize zein gene, the oat glutelin gene, the Sorghumkasirin-gene, the rye secalin gene).

In order to ensure the stable integration, into the transgenic plant, ofnucleic acid molecules used in the process according to the invention incombination with further biosynthesis genes over a plurality ofgenerations, each of the coding regions used in the process should beexpressed under the control of its own, preferably unique, promotersince repeating sequence motifs may lead to recombination events or tosilencing or, in plants, to instability of the T-DNA.

The nucleic acid construct is advantageously constructed in such a waythat a promoter is followed by a suitable cleavage site for insertion ofthe nucleic acid to be expressed, advantageously in a polylinker,followed, if appropriate, by a terminator located behind the polylinker.If appropriate, this order is repeated several times so that severalgenes are combined in one construct and thus can be introduced into thetransgenic plant in order to be expressed. The sequence isadvantageously repeated up to three times. For the expression, thenucleic acid sequences are inserted via the suitable cleavage site, forexample in the polylinker behind the promoter. It is advantageous foreach nucleic acid sequence to have its own promoter and, if appropriate,its own terminator, as mentioned above. However, it is also possible toinsert several nucleic acid sequences behind a promoter and, ifappropriate, before a terminator if a polycistronic transcription ispossible in the host or target cells. In this context, the insertionsite, or the sequence of the nucleic acid molecules inserted, in thenucleic acid construct is not decisive, that is to say a nucleic acidmolecule can be inserted in the first or last position in the cassettewithout this having a substantial effect on the expression. However, itis also possible to use only one promoter type in the construct.However, this may lead to undesired recombination events or silencingeffects, as said.

Accordingly, in a preferred embodiment, the nucleic acid constructaccording to the invention confers expression of the nucleic acidmolecule of the invention, and, optionally further genes, in a plant andcomprises one or more plant regulatory elements. Said nucleic acidconstruct according to the invention advantageously encompasses a plantpromoter or a plant terminator or a plant promoter and a plantterminator.

A “plant” promoter comprises regulatory elements, which mediate theexpression of a coding sequence segment in plant cells. Accordingly, aplant promoter need not be of plant origin, but may originate fromviruses or microorganisms, in particular for example from viruses whichattack plant cells.

The plant promoter can also originate from a plant cell, e.g. from theplant, which is transformed with the nucleic acid construct or vector asdescribed herein. This also applies to other “plant” regulatory signals,for example in “plant” terminators.

A nucleic acid construct suitable for plant expression preferablycomprises regulatory elements which are capable of controlling theexpression of genes in plant cells and which are operably linked so thateach sequence can fulfill its function. Accordingly, the nucleic acidconstruct can also comprise transcription terminators. Examples fortranscriptional termination are polyadenylation signals. Preferredpolyadenylation signals are those which originate from Agrobacteriumtumefaciens T-DNA, such as the gene 3 of the Ti plasmid pTiACH5, whichis known as octopine synthase (Gielen et al., EMBO J. 3 (1984) 835 etseq.) or functional equivalents thereof, but all the other terminatorswhich are functionally active in plants are also suitable.

The nucleic acid construct suitable for plant expression preferably alsocomprises other operably linked regulatory elements such as translationenhancers, for example the overdrive sequence, which comprises thetobacco mosaic virus 5′-untranslated leader sequence, which increasesthe protein/RNA ratio (Gallie et al., 1987, Nucl. Acids Research15:8693-8711).

For expression in plants, the nucleic acid molecule must, as describedabove, be linked operably to or comprise a suitable promotor whichexpresses the gene at the right point in time and in a cell- ortissue-specific manner. Usable promoters are constitutive promoters(Benfey et al., EMBO J. 8 (1989) 2195-2202), such as those whichoriginate from plant viruses, such as 35S CAMV (Franck et al., Cell 21(1980) 285-294), 19S CaMV (see also U.S. Pat. No. 5,352,605 and WO84/02913), 34S FMV (Sanger et al., Plant. Mol. Biol., 14, 1990:433-443), the parsley ubiquitin promoter, or plant promoters such as theRubisco small subunit promoter described in U.S. Pat. No. 4,962,028 orthe plant promoters PRP1 [Ward et al., Plant. Mol. Biol. 22 (1993)],SSU, PGEL1, OCS [Leisner (1988) Proc Natl Acad Sci USA 85(5):2553-2557], lib4, usp, mas [Comai (1990) Plant Mol Biol 15 (3):373-381],STLS1, ScBV (Schenk (1999) Plant Mol Biol 39(6):1221-1230), B33, SAD1 orSAD2 (flax promoters, Jain et al., Crop Science, 39 (6), 1999:1696-1701) or nos [Shaw et al. (1984) Nucleic Acids Res.12(20):7831-7846]. Stable, constitutive expression of the proteinsaccording to the invention in a plant can be advantageous. However,inducible expression of the polypeptide of the invention isadvantageous, if a late expression before the harvest is of advantage,as metabolic manipulation may lead to plant growth retardation.

The expression of plant genes can also be facilitated as described abovevia a chemical inducible promoter (for a review, see Gatz 1997, Annu.Rev. Plant Physiol. Plant Mol. Biol., 48:89-108). Chemically induciblepromoters are particularly suitable when it is desired to express thegene in a time-specific manner. Examples of such promoters are asalicylic acid inducible promoter (WO 95/19443), and abscisicacid-inducible promoter (EP 335 528), a tetracyclin-inducible promoter(Gatz et al. (1992) Plant J. 2, 397-404), a cyclohexanol- orethanol-inducible promoter (WO 93/21334) or others as described herein.

Other suitable promoters are those which react to biotic or abioticstress conditions, for example the pathogen-induced PRP1 gene promoter(Ward et al., Plant. Mol. Biol. 22 (1993) 361-366), the tomatoheat-inducible hsp80 promoter (U.S. Pat. No. 5,187,267), the potatochill-inducible alpha-amylase promoter (WO 96/12814) or thewound-inducible pinII promoter (EP-A-0 375 091) or others as describedherein.

Preferred promoters are in particular those which bring about geneexpression in tissues and organs in which the biosynthesis of aminoacids takes place, in seed cells, such as endosperm cells and cells ofthe developing embryo. Suitable promoters are the oilseed rape napingene promoter (U.S. Pat. No. 5,608,152), the Vicia faba USP promoter(Baeumlein et al., Mol Gen Genet, 1991, 225 (3): 459-67), theArabidopsis oleosin promoter (WO 98/45461), the Phaseolus vulgarisphaseolin promoter (U.S. Pat. No. 5,504,200), the Brassica Bce4 promoter(WO 91/13980), the bean arc5 promoter, the carrot DcG3 promoter, or theLegumin B4 promoter (LeB4; Baeumlein et al., 1992, Plant Journal, 2 (2):233-9), and promoters which bring about the seed-specific expression inmonocotyledonous plants such as maize, barley, wheat, rye, rice and thelike. Advantageous seed-specific promoters are the sucrose bindingprotein promoter (WO 00/26388), the phaseolin promoter and the napinpromoter. Suitable promoters which must be considered are the barleylpt2 or lpt1 gene promoter (WO 95/15389 and WO 95/23230), and thepromoters described in WO 99/16890 (promoters from the barley hordeingene, the rice glutelin gene, the rice oryzin gene, the rice prolamingene, the wheat gliadin gene, the wheat glutelin gene, the maize zeingene, the oat glutelin gene, the sorghum kasirin gene and the ryesecalin gene). Further suitable promoters are Amy32b, Amy 6-6 andAleurain [U.S. Pat. No. 5,677,474], Bce4 (oilseed rape) [U.S. Pat. No.5,530,149], glycinin (soya) [EP 571 741], phosphoenolpyruvatecarboxylase (soya) [JP 06/62870], ADR12-2 (soya) [WO 98/08962],isocitrate lyase (oilseed rape) [U.S. Pat. No. 5,689,040] or α-amylase(barley) [EP 781 849]. Other promoters which are available for theexpression of genes in plants are leaf-specific promoters such as thosedescribed in DE-A 19644478 or light-regulated promoters such as, forexample, the pea petE promoter.

Further suitable plant promoters are the cytosolic FBPase promoter orthe potato ST-LSI promoter (Stockhaus et al., EMBO J. 8, 1989, 2445),the Glycine max phosphoribosylpyrophosphate amidotransferase promoter(GenBank Accession No. U87999) or the node-specific promoter describedin EP-A-0 249 676.

Other promoters, which are particularly suitable are those which bringabout plastid-specific expression. Suitable promoters such as the viralRNA polymerase promoter are described in WO 95/16783 and WO 97/06250,and the Arabidopsis clpP promoter, which is described in WO 99/46394.

Other promoters, which are used for the strong expression ofheterologous sequences in as many tissues as possible, in particularalso in leaves, are, in addition to several of the abovementioned viraland bacterial promoters, preferably, plant promoters of actin orubiquitin genes such as, for example, the rice actin1 promoter. Furtherexamples of constitutive plant promoters are the sugarbeet V-ATPasepromoters (WO 01/14572). Examples of synthetic constitutive promotersare the Super promoter (WO 95/14098) and promoters derived from G-boxes(WO 94/12015). If appropriate, chemical inducible promoters mayfurthermore also be used, compare EP-A 388186, EP-A 335528, WO 97/06268.

Another embodiment of the invention is a nucleic acid constructconferring the expression of the dsRNA molecule, the antisense nucleicacid molecule, the ribozyme, the viral nucleic acid molecule or thenucleic acid molecule as used in the inventive process, suitable for theexpression in microorganism or preferably in plant.

Preferred recipient plants are, as described above, in particular thoseplants, which can be transformed in a suitable manner. These includemonocotyle-donous and dicotyledonous plants. Plants which must bementioned in particular are agriculturally useful plants such as cerealsand grasses, for example Triticum spp., Zea mays, Hordeum vulgare, oats,Secale cereale, Oryza sativa, Pennisetum glaucum, Sorghum bicolor,Triticale, Agrostis spp., Cenchrus ciliaris, Dactylis glomerata, Festucaarundinacea, Lolium spp., Medicago spp. and Saccharum spp., legumes andoil crops, for example Brassica juncea, Brassica napus, Glycine max,Arachis hypogaea, Gossypium hirsutum, Cicer arietinum, Helianthusannuus, Lens culinaris, Linum usitatissimum, Sinapis alba, Trifoliumrepens and Vicia narbonensis, vegetables and fruits, for examplebananas, grapes, Lycopersicon esculentum, asparagus, cabbage,watermelons, kiwi fruit, Solanum tuberosum, Beta vulgaris, cassaya andchicory, trees, for example Coffea species, Citrus spp., Eucalyptusspp., Picea spp., Pinus spp. and Populus spp., medicinal plants andtrees, and flowers.

One embodiment of the present invention also relates to a method forgenerating a vector, which comprises the insertion, into a vector, ofthe nucleic acid molecule characterized herein, the nucleic acidmolecule according to the invention or the expression cassette accordingto the invention. The vector can, for example, be introduced into acell, e.g. a microorganism or a plant cell, as described herein for thenucleic acid construct, or below under transformation or transfection orshown in the examples. A transient or stable transformation of the hostor target cell is possible, however, a stable transformation ispreferred.

The vector according to the invention is preferably a vector, which issuitable for reducing, decreasing or deleting of the polypeptideaccording to the invention in a plant. The method can thus alsoencompass one or more steps for integrating regulatory signals into thevector, in particular signals, which mediate the reduction, decrease ordeletion in an organism such as a microorganism or plant.

Accordingly, the present invention also relates to a vector comprisingthe nucleic acid molecule characterized herein as part of a nucleic acidconstruct suitable for plant expression or the nucleic acid moleculeaccording to the invention.

The advantageous vectors of the invention comprise the nucleic acidmolecules which encode proteins according to the invention, nucleic acidmolecules which are used in the process, or nucleic acid constructsuitable for the expression in microorganism or plant as described abovecomprising the nucleic acid molecules used, either alone or incombination with further genes such as the biosynthesis or regulatorygenes of the amino acid metabolism e.g. with the genes mentioned hereinabove. In accordance with the invention, the term “vector” refers to anucleic acid molecule, which is capable of transporting another nucleicacid to which it is linked. One type of vector is a “plasmid”, whichmeans a circular double-stranded DNA loop into which additional DNAsegments can be ligated. A further type of vector is a viral vector, itbeing possible to ligate additional DNA segments into the viral genome.Certain vectors are capable of autonomous replication in a host cellinto which they have been introduced (for example bacterial vectors withbacterial replication origin). Other preferred vectors areadvantageously completely or partly integrated into the genome of a hostcell when they are introduced into the host cell and thus replicatetogether with the host genome. Moreover, certain vectors are capable ofcontrolling the expression of genes with which they are in operablelinkage. In the present context, these vectors are referred to as“expression vectors”. As mentioned above, they are capable of autonomousreplication or may be integrated partly or completely into the hostgenome. Expression vectors, which are suitable for DNA recombinationtechniques usually, take the form of plasmids. In the presentdescription, “plasmid” and “vector” can be used interchangeably sincethe plasmid is the most frequently used form of a vector. However, theinvention is also intended to encompass these other forms of expressionvectors, such as viral vectors, which exert similar functions. The termvector is furthermore also to encompass other vectors which are known tothe skilled worker, such as phages, viruses such as SV40, CMV, TMV,transposons, IS elements, phasmids, phagemids, cosmids, and linear orcircular DNA.

The recombinant expression vectors which are advantageously used in theprocess comprise the nucleic acid molecules according to the inventionor the nucleic acid construct according to the invention in a form whichis suitable for repressing the nucleic acid molecules of the inventionand/or in the same time expressing, in a host cell, additional genes,which are accompanied by the nucleic acid molecules according to theinvention or described herein. Accordingly, the recombinant expressionvectors comprise one or more regulatory signals selected on the basis ofthe host cells to be used for the expression, in operable linkage withthe nucleic acid sequence to be expressed.

In a recombinant expression vector, “operable linkage” means that thenucleic acid molecule of interest is linked to the regulatory signals insuch a way that expression of the genes is possible: they are linked toone another in such a way that the two sequences fulfill the predictedfunction assigned to the sequence (for example in an in-vitrotranscription/translation system, or in a host cell if the vector isintroduced into the host cell).

The term “regulatory sequence” is intended to comprise promoters,enhancers and other expression control elements (for examplepolyadenylation signals). These regulatory sequences are described, forexample, in Goeddel: Gene Expression Technology Methods in Enzymology185, Academic Press, San Diego, Calif. (1990), or see: Gruber andCrosby, in: Methods in Plant Molecular Biology and Biotechnology, CRCPress, Boca Raton, Fla., Ed.: Glick and Thompson, chapter 7, 89-108,including the references cited therein. Regulatory sequences encompassthose, which control the constitutive expression of a nucleotidesequence in many types of host cells and those which control the directexpression of the nucleotide sequence in specific host cells only, andunder specific conditions. The skilled worker knows that the design ofthe expression vector may depend on factors such as the selection of thehost cell to be transformed, the extent to which the desired protein isexpressed, and the like. A preferred selection of regulatory sequencesis described above, for example promoters, terminators, enhancers andthe like. The term regulatory sequence is to be considered as beingencompassed by the term regulatory signal. Several advantageousregulatory sequences, in particular promoters and terminators aredescribed above. In general, the regulatory sequences described asadvantageous for nucleic acid construct suitable for expression are alsoapplicable for vectors.

The recombinant expression vectors used can be designed specifically forthe expression, in prokaryotic and/or eukaryotic cells, of nucleic acidmolecules used in the process. This is advantageous since intermediatesteps of the vector construction are frequently carried out inmicroorganisms for the sake of simplicity. For example, the genesaccording to the invention and other genes can be expressed in bacterialcells, insect cells (using baculovirus expression vectors), yeast cellsand other fungal cells [Romanos (1992), Yeast 8:423-488; van den Hondel,(1991), in: More Gene Manipulations in Fungi, J. W. Bennet & L. L.Lasure, Ed., pp. 396-428: Academic Press: San Diego; and van den Hondel,C.A.M.J.J. (1991), in: Applied Molecular Genetics of Fungi, Peberdy, J.F., et al., Ed., pp. 1-28, Cambridge University Press: Cambridge], algae[Falciatore et al., 1999, Marine Biotechnology. 1, 3:239-251] usingvectors and following a transformation method as described in WO98/01572, and preferably in cells of multi-celled plants [see Schmidt,R. and Willmitzer, L. (1988) Plant Cell Rep.: 583-586; Plant MolecularBiology and Biotechnology, C Press, Boca Raton, Fla., chapter 6/7, pp.71-119 (1993); F. F. White, in: Transgenic Plants, Bd. 1, Engineeringand Utilization, Ed.: Kung and R. Wu, Academic Press (1993), 128-43;Potrykus, Annu. Rev. Plant Physiol. Plant Molec. Biol. 42 (1991),205-225 (and references cited therein)]. Suitable host cells arefurthermore discussed in Goeddel, Gene Expression Technology: Methods inEnzymology 185, Academic Press, San Diego, Calif. (1990). As analternative, the sequence of the recombinant expression vector can betranscribed and translated in vitro, for example using T7promotor-regulatory sequences and T7 polymerase.

In most cases, proteins can be expressed in prokaryotes using vectorscomprising constitutive or inducible promoters, which control theexpression of fusion proteins or nonfusion proteins. Typical fusionexpression vectors are, inter alia, pGEX (Pharmacia Biotech Inc; Smith,D. B., and Johnson, K. S. (1988) Gene 67:31-40), pMAL (New EnglandBiolabs, Beverly, Mass.) and pRIT5 (Pharmacia, Piscataway, N.J.), inwhich glutathione-S-transferase (GST), maltose-E-binding protein orprotein A is fused with the recombinant target protein. Examples ofsuitable inducible nonfusion E. coli expression vectors are, inter alia,pTrc (Amann et al. (1988) Gene 69:301-315) and pET 11d [Studier et al.,Gene Expression Technology: Methods in Enzymology 185, Academic Press,San Diego, Calif. (1990) 60-89]. The target gene expression of the pTrcvector is based on the transcription of a hybrid trp-lac fusion promoterby the host RNA polymerase. The target gene expression from the pET 11dvector is based on the transcription of a T7-gn10-lac fusion promoter,which is mediated by a coexpressed viral RNA polymerase (T7 gn1). Thisviral polymerase is provided by the host strains BL21 (DE3) or HMS174(DE3) by a resident □-prophage, which harbors a T7 gn1 gene under thetranscriptional control of the lacUV 5 promoter.

Other vectors which are suitable in prokaryotic organisms are known tothe skilled worker; these vectors are for example in E. coli pLG338,pACYC184, the pBR series, such as pBR322, the pUC series such as pUC18or pUC19, the M113mp series, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24,pLG200, pUR290, pIN-III¹¹³-B1, □gt11 or pBdCl, in Streptomyces pIJ101,pIJ364, pIJ702 or pIJ361, in Bacillus pUB110, pC194 or pBD214, inCorynebacterium pSA77 or pAJ667.

In a further embodiment, the expression vector is a yeast expressionvector. Examples of vectors for expression in the yeasts S. cerevisiaeencompass pYeDesaturasec1 (Baldari et al. (1987) Embo J. 6:229-234),pMFa (Kurjan and Herskowitz (1982) Cell 30:933-943), pJRY88 (Schultz etal. (1987) Gene 54:113-123) and pYES2 (Invitrogen Corporation, SanDiego, Calif.). Vectors and methods for the construction of vectorswhich are suitable for use in other fungi, such as the filamentousfungi, encompass those which are described in detail in: van den Hondel,C.A.M.J.J. [(1991), J. F. Peberdy, Ed., pp. 1-28, Cambridge UniversityPress: Cambridge; or in: More Gene Manipulations in Fungi; J. W. Bennet& L. L. Lasure, Ed., pp. 396-428: Academic Press: San Diego]. Examplesof other suitable yeast vectors are 2□M, pAG-1, YEp6, YEp13 orpEMBLYe23.

Further vectors, which may be mentioned by way of example, are pALS1,pIL2 or pBB116 in fungi or pLGV23, pGHlac⁺, pBIN19, pAK2004 or pDH51 inplants.

As an alternative, the nucleic acid sequences can be expressed in insectcells using baculovirus expression vectors. Baculovirus vectors whichare available for expressing proteins in cultured insect cells (forexample Sf9 cells) encompass the pAc series (Smith et al. (1983) Mol.Cell Biol. 3:2156-2165) and the pVL series (Lucklow and Summers (1989)Virology 170:31-39).

The abovementioned vectors are only a small overview of potentiallysuitable vectors. Further plasmids are known to the skilled worker andare described, for example, in: Cloning Vectors (Ed. Pouwels, P. H., etal., Elsevier, Amsterdam-New York-Oxford, 1985, ISBN 0 444 904018).Further suitable expression systems for prokaryotic and eukaryoticcells, see the chapters 16 and 17 by Sambrook, J., Fritsch, E. F., andManiatis, T., Molecular Cloning: A Laboratory Manual, 2nd Edition, ColdSpring Harbor Laboratory, Cold Spring Harbor Laboratory Press, ColdSpring Harbor, N.Y., 1989.

Accordingly, one embodiment of the invention relates to a vectorcomprising the nucleic acid molecule according to the invention or anucleic acid construct of the invention. Said vector is useful for thereduction, decrease or deletion of the polypeptide according to theinvention in an organism preferably a plant. Advantageously the nucleicacid molecule of the invention is in an operable linkage with regulatorysequences for the expression in a prokaryotic or eukaryotic, or in aprokaryotic and eukaryotic host. Furthermore vectors which are suitablefor homologous recombination are also within the scope of the invention.

Accordingly, one embodiment of the invention relates to a host cell,which has been transformed stably or transiently with the vectoraccording to the invention or the nucleic acid molecule according to theinvention or the nucleic acid construct according to the invention. Saidhost cell may be a microorganism, a non-human animal cell or a plantcell.

Depending on the host organism, the organisms used in the processaccording to the invention are cultured or grown in a manner with whichthe skilled worker is familiar. As a rule, microorganisms are grown in aliquid medium comprising a carbon source, usually in the form of sugars,a nitrogen source, usually in the form of organic nitrogen sources suchas yeast extract or salts such as ammonium sulfate, trace elements suchas iron salts, manganese salts, magnesium salts, and, if appropriate,vitamins, at temperatures between 0° C. and 100° C., preferably between10° C. and 60° C., while passing in oxygen. In the event themicroorganism is anaerobe, no oxygen is blown through the culturemedium. The pH value of the liquid nutrient medium may be kept constant,that is to say regulated during the culturing phase, or not. Theorganisms may be cultured batchwise, semibatchwise or continuously.Nutrients may be provided at the beginning of the fermentation or fed insemi-continuously or continuously.

The amino acids produced can be isolated from the organism by methodswith which the skilled worker is familiar. For example via extraction,salt precipitation and/or ion-exchange chromatography. To this end, theorganisms may be disrupted beforehand. The process according to theinvention can be conducted batchwise, semibatchwise or continuously. Asummary of known culture and isolation techniques can be found in thetextbook by Chmiel [Bioprozeβtechnik 1, Einführung in dieBioverfahrenstechnik (Gustav Fischer Verlag, Stuttgart, 1991)], Demainet al. (Industrial Microbiology and Biotechnology, second edition, ASMPress, Washington, D.C., 1999, ISBN 1-55581-128-0] or in the textbook byStorhas (Bioreaktoren und periphere Einrichtungen (Vieweg Verlag,Braunschweig/Wiesbaden, 1994)).

In one embodiment, the present invention relates to a polypeptideencoded by the nucleic acid molecule according to the present invention,preferably conferring an increase in the fine chemical content in anorganism or cell after decreasing the expression or activity.

The present invention also relates to a process for the production of apolypeptide according to the present invention, the polypeptide beingexpressed in a host cell according to the invention, preferably in amicroorganism, non-human animal cell or a transgenic plant cell.

In one embodiment, the nucleic acid molecule used in the process for theproduction of the polypeptide is derived from a microorganism,preferably from a prokaryotic or protozoic cell with a eukaryoticorganism as host cell. E.g., in one embodiment the polypeptide isproduced in a plant cell or plant with a nucleic acid molecule derivedfrom a prokaryote or a fungus or an alga or another microorganism butnot from plant.

The skilled worker knows that protein and DNA expressed in differentorganisms differ in many respects and properties, e.g. methylation,degradation and post-translational modification as for exampleglucosylation, phosphorylation, acetylation, myristoylation,ADP-ribosylation, farnesylation, carboxylation, sulfation, ubiquination,etc. though having the same coding sequence. Preferably, the cellularexpression control of the corresponding protein differs accordingly inthe control mechanisms controlling the activity and expression of anendogenous protein or another eukaryotic protein. One major differencebetween proteins expressed in prokaryotic or eukaryotic organism is theamount of glycosylation. For example in E. coli there are noglycosylated proteins. Proteins expressed in yeasts have high mannosecontent in the glycosylated proteins, whereas in plants theglycosylation pattern is complex.

The polypeptide of the present invention is preferably produced byrecombinant DNA techniques. For example, a nucleic acid moleculeencoding the protein is cloned into a vector (as described above), thevector is introduced into a host cell (as described above) and saidpolypeptide is expressed in the host cell. Said polypeptide can then beisolated from the cells by an appropriate purification scheme usingstandard protein purification techniques. Alternative to recombinantexpression, the polypeptide or peptide of the present invention can besynthesized chemically using standard peptide synthesis techniques.Moreover, native polypeptide having the biological activity of theprotein of the invention can be isolated from cells (e.g., endothelialcells), for example using the antibody of the present invention asdescribed below, in particular, an anti-protein of the inventionantibody, which can be produced by standard techniques utilizing thepolypeptide of the present invention or fragment thereof, i.e., thepolypeptide of this invention.

In one embodiment, the present invention relates to a polypeptide havingthe biological activity represented by the protein of the invention. Inone embodiment, said polypeptide having the biological activityrepresented by the protein of the invention distinguishes over thesequence depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ IDNO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ IDNO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ IDNO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115,SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ IDNO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ IDNO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ IDNO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ IDNO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ IDNO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ IDNO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ IDNO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ IDNO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ IDNO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305,SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ IDNO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ IDNO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ IDNO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ IDNO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ IDNO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ IDNO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ IDNO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 by one or more aminoacids. In another embodiment, said polypeptide of the invention does notconsist of the sequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO:6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441. In a furtherembodiment, said polypeptide of the present invention is less than 100%,99.999%, 99.99%, 99.9% or 99% identical identical to SEQ ID NO: 2, SEQID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24,SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO:65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ IDNO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93,SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO:103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO:121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO:139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO:164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO:182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO:200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO:218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO:243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO:266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO:293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO:311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO:329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO:347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO:365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO:383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO:401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO:419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO:441.

In one embodiment, the present invention relates to a polypeptide havingthe amino acid sequence encoded by a nucleic acid molecule of theinvention or obtainable by a process of the invention. Said polypeptideconfers preferably the aforementioned activity, in particular, thepolypeptide confers the increase of the fine chemical in a cell or anorganism or a part thereof after decreasing the cellular activity, e.g.by decreasing the expression or the specific activity of thepolypeptide. In one embodiment, said polypeptide distinguishes over thesequence depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ IDNO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28,SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO:69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ IDNO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97,SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ IDNO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115,SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ IDNO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133,SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ IDNO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158,SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ IDNO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176,SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ IDNO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194,SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ IDNO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212,SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ IDNO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237,SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ IDNO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260,SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ IDNO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287,SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ IDNO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305,SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ IDNO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323,SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ IDNO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341,SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ IDNO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359,SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ IDNO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377,SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ IDNO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395,SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ IDNO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413,SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ IDNO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431,SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 by one or more aminoacids. Preferably, the sequence of the polypeptide of the inventiondistinguishes from the sequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26,SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO:67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ IDNO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95,SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO:105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO:123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO:141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO:166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO:184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQID NO: 194, SEQ ID NO: 196, SEQ ID NO: 193, SEQ ID NO: 200, SEQ ID NO:202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO:220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO:250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO:268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO:295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO:331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO:349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO:385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO:403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO:421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 by not morethan 80% or 70% of the amino acids, preferably not more than 60% or 50%,more preferred not more than 40% or 30%, even more preferred not morethan 20% or 10%. In one embodiment, polypeptide distinguishes form thesequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ IDNO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ IDNO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO:107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO:297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 by more than 5, 6, 7, 8 or9 amino acids, preferably by more than 10, 15, 20, 25 or 30 amino acids,even more preferred are more than 40, 50, or 60 amino acids. In anotherembodiment, said polypeptide of the invention does not consist of thesequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ IDNO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ IDNO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO:107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO:297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441. In a further embodiment,said polypeptide of the present invention is less than 100%, 99.999%,99.99%, 99.9% or 99% identical. In one embodiment, the polypeptide ofthe invention originates from an non-plant cell, in particular from amicroorganism, and was expressed in a plant cell.

The terms “protein” and “polypeptide” used in this application areinterchangeable. “Polypeptide” refers to a polymer of amino acids (aminoacid sequence) and does not refer to a specific length of the molecule.Thus peptides and oligopeptides are included within the definition ofpolypeptide. This term does also refer to or include post-translationalmodifications of the polypeptide, for example, glycosylations,acetylations, phosphorylations and the like. Included within thedefinition are, for example, polypeptides containing one or more analogsof an amino acid (including, for example, unnatural amino acids, etc.),polypeptides with substituted linkages, as well as other modificationsknown in the art, both naturally occurring and non-naturally occurring.

Preferably, the polypeptide is isolated. An “isolated” or “purified”protein or nucleic acid molecule or biologically active portion thereofis substantially free of cellular material when produced by recombinantDNA techniques, or chemical precursors or other chemicals whenchemically synthesized.

The language “substantially free of cellular material” includespreparations of the polypeptide of the invention in which the protein isseparated from cellular components of the cells in which it is naturallyor recombinantly produced. In one embodiment, the language“substantially free of cellular material” includes preparations havingless than about 30% (by dry weight) of “contaminating protein”, morepreferably less than about 20% of “contaminating protein”, still morepreferably less than about 10% of “contaminating protein”, and mostpreferably less than about 5% “contaminating protein”. The term“contaminating protein” relates to polypeptides, which are notpolypeptides of the present invention. When the polypeptide of thepresent invention or biologically active portion thereof isrecombinantly produced, it is also preferably substantially free ofculture medium, i.e., culture medium represents less than about 20%,more preferably less than about 10%, and most preferably less than about5% of the volume of the protein preparation. The language “substantiallyfree of chemical precursors or other chemicals” includes preparations inwhich the polypeptide of the present invention is separated fromchemical precursors or other chemicals, which are involved in thesynthesis of the protein. The language “substantially free of chemicalprecursors or other chemicals” includes preparations having less thanabout 30% (by dry weight) of chemical precursors or other proteins orchemicals which are not identical to the protein of the invention, morepreferably less than about 20% chemical precursors or other proteins orchemicals, still more preferably less than about 10% chemical precursorsor other proteins or chemicals, and most preferably less than about 5%chemical precursors or other proteins or chemicals which are notidentical to the protein of the invention. In preferred embodiments,isolated proteins or biologically active portions thereof lackcontaminating proteins from the same organism from which the polypeptideof the present invention is derived. Typically, such proteins areproduced by recombinant techniques.

A polypeptide of the invention can participate in the process of thepresent invention. The polypeptide or a portion thereof comprisespreferably an amino acid sequence which is sufficiently homologous to anamino acid sequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 such that theprotein or portion thereof maintains the ability to confer the activityof the present invention, that means an increase of the fine chemicalcontent in the organism by decreasing its biological activity.Preferably, the polypeptide used in the inventive process has an aminoacid sequence identical as shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ IDNO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ IDNO: 16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQID NO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67,SEQ ID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO:77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ IDNO: 87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQID NO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO:105, SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQID NO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO:123, SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO:141, SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQID NO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO:166, SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQID NO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO:184, SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQID NO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO:202, SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQID NO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO:220, SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQID NO: 237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO:250, SEQ ID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQID NO: 260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO:268, SEQ ID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQID NO: 287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO:295, SEQ ID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQID NO: 305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO:313, SEQ ID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQID NO: 323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO:331, SEQ ID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQID NO: 341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO:349, SEQ ID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQID NO: 359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO:367, SEQ ID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQID NO: 377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO:385, SEQ ID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQID NO: 395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO:403, SEQ ID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQID NO: 413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO:421, SEQ ID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQID NO: 431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441.

Further, the polypeptide can have an amino acid sequence which isencoded by a nucleotide sequence which hybridizes, preferably hybridizesunder stringent conditions as described above, to a nucleotide sequenceof the nucleic acid molecule of the present invention. Accordingly, thepolypeptide has an amino acid sequence which is encoded by a nucleotidesequence that is at least about 35%, 40%, 45%, 50%, 55%, 60%, 65% or70%, preferably at least about 75%, 80%, 85% or 90%, and more preferablyat least about 91%, 92%, 93%, 94% or 95%, and even more preferably atleast about 96%, 97%, 98%, 99% or more homologous to one of the aminoacid sequences of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO:8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ IDNO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69,SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO:79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ IDNO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO:107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO:125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO:143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO:168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO:186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO:204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO:229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO:252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO:270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO:297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO:315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO:333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO:351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO:369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO:387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO:405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO:423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441. The preferred polypeptideof the present invention preferably possesses at least one of theactivities according to the invention and described herein. A preferredpolypeptide of the present invention includes an amino acid sequenceencoded by a nucleotide sequence which hybridizes, preferably hybridizesunder stringent conditions, to a nucleotide sequence of SEQ ID NO: 1,SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 9, SEQ ID NO: 11,SEQ ID NO: 13, SEQ ID NO: 15, SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO:23, SEQ ID NO: 25, SEQ ID NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ IDNO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQID NO: 74, SEQ ID NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82,SEQ ID NO: 84, SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO:92, SEQ ID NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ IDNO: 102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ IDNO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID NO: 128,SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO: 136, SEQ IDNO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142, SEQ ID NO: 151,SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ ID NO: 159, SEQ IDNO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID NO: 167, SEQ ID NO: 169,SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO: 175, SEQ ID NO: 177, SEQ IDNO: 179, SEQ ID NO: 181, SEQ ID NO: 183, SEQ ID NO: 185, SEQ ID NO: 187,SEQ ID NO: 189, SEQ ID NO: 191, SEQ ID NO: 193, SEQ ID NO: 195, SEQ IDNO: 197, SEQ ID NO: 199, SEQ ID NO: 201, SEQ ID NO: 203, SEQ ID NO: 205,SEQ ID NO: 207, SEQ ID NO: 209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ IDNO: 215, SEQ ID NO: 217, SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230,SEQ ID NO: 232, SEQ ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ IDNO: 240, SEQ ID NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253,SEQ ID NO: 255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ IDNO: 263, SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271,SEQ ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ IDNO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO: 298,SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306, SEQ IDNO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ ID NO: 316,SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID NO: 324, SEQ IDNO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO: 332, SEQ ID NO: 334,SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340, SEQ ID NO: 342, SEQ IDNO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ ID NO: 350, SEQ ID NO: 352,SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID NO: 358, SEQ ID NO: 360, SEQ IDNO: 362, SEQ ID NO: 364, SEQ ID NO: 366, SEQ ID NO: 368, SEQ ID NO: 370,SEQ ID NO: 372, SEQ ID NO: 374, SEQ ID NO: 376, SEQ ID NO: 378, SEQ IDNO: 380, SEQ ID NO: 382, SEQ ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388,SEQ ID NO: 390, SEQ ID NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ IDNO: 398, SEQ ID NO: 400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406,SEQ ID NO: 408, SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ IDNO: 416, SEQ ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424,SEQ ID NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ IDNO: 434 or SEQ ID NO: 440 or which is homologous thereto, as definedabove.

Accordingly the polypeptide of the present invention can vary from SEQID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6, SEQ ID NO: 8, SEQ ID NO: 10, SEQID NO: 12, SEQ ID NO: 14, SEQ ID NO: 16, SEQ ID NO: 20, SEQ ID NO: 22,SEQ ID NO: 24, SEQ ID NO: 26, SEQ ID NO: 28, SEQ ID NO. 30, SEQ ID NO:32; SEQ ID NO: 65, SEQ ID NO: 67, SEQ ID NO: 69, SEQ ID NO: 71, SEQ IDNO: 73, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89, SEQ ID NO: 91,SEQ ID NO: 93, SEQ ID NO: 95, SEQ ID NO: 97, SEQ ID NO: 99, SEQ ID NO:101, SEQ ID NO: 103, SEQ ID NO: 105, SEQ ID NO: 107, SEQ ID NO: 109, SEQID NO: 111, SEQ ID NO: 113, SEQ ID NO: 115, SEQ ID NO: 117, SEQ ID NO:119, SEQ ID NO: 121, SEQ ID NO: 123, SEQ ID NO: 125, SEQ ID NO: 127, SEQID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO:137, SEQ ID NO: 139, SEQ ID NO: 141, SEQ ID NO: 143, SEQ ID NO: 152, SEQID NO: 154, SEQ ID NO: 156, SEQ ID NO: 158, SEQ ID NO: 160, SEQ ID NO:162, SEQ ID NO: 164, SEQ ID NO: 166, SEQ ID NO: 168, SEQ ID NO: 170, SEQID NO: 172, SEQ ID NO: 174, SEQ ID NO: 176, SEQ ID NO: 178, SEQ ID NO:180, SEQ ID NO: 182, SEQ ID NO: 184, SEQ ID NO: 186, SEQ ID NO: 188, SEQID NO: 190, SEQ ID NO: 192, SEQ ID NO: 194, SEQ ID NO: 196, SEQ ID NO:198, SEQ ID NO: 200, SEQ ID NO: 202, SEQ ID NO: 204, SEQ ID NO: 206, SEQID NO: 208, SEQ ID NO: 210, SEQ ID NO: 212, SEQ ID NO: 214, SEQ ID NO:216, SEQ ID NO: 218, SEQ ID NO: 220, SEQ ID NO: 229, SEQ ID NO:231, SEQID NO:233, SEQ ID NO: 235, SEQ ID NO: 237, SEQ ID NO: 239, SEQ ID NO:241, SEQ ID NO: 243, SEQ ID NO: 250, SEQ ID NO: 252, SEQ ID NO: 254, SEQID NO: 256, SEQ ID NO: 258, SEQ ID NO: 260, SEQ ID NO: 262, SEQ ID NO:264, SEQ ID NO: 266, SEQ ID NO: 268, SEQ ID NO: 270, SEQ ID NO: 272, SEQID NO: 283, SEQ ID NO: 285, SEQ ID NO: 287, SEQ ID NO: 289, SEQ ID NO:291, SEQ ID NO: 293, SEQ ID NO: 295, SEQ ID NO: 297, SEQ ID NO: 299, SEQID NO: 301, SEQ ID NO: 303, SEQ ID NO: 305, SEQ ID NO: 307, SEQ ID NO:309, SEQ ID NO: 311, SEQ ID NO: 313, SEQ ID NO: 315, SEQ ID NO: 317, SEQID NO: 319, SEQ ID NO: 321, SEQ ID NO: 323, SEQ ID NO: 325, SEQ ID NO:327, SEQ ID NO: 329, SEQ ID NO: 331, SEQ ID NO: 333, SEQ ID NO: 335, SEQID NO: 337, SEQ ID NO: 339, SEQ ID NO: 341, SEQ ID NO: 343, SEQ ID NO:345, SEQ ID NO: 347, SEQ ID NO: 349, SEQ ID NO: 351, SEQ ID NO: 353, SEQID NO: 355, SEQ ID NO: 357, SEQ ID NO: 359, SEQ ID NO: 361, SEQ ID NO:363, SEQ ID NO: 365, SEQ ID NO: 367, SEQ ID NO: 369, SEQ ID NO: 371, SEQID NO: 373, SEQ ID NO: 375, SEQ ID NO: 377, SEQ ID NO: 379, SEQ ID NO:381, SEQ ID NO: 383, SEQ ID NO: 385, SEQ ID NO: 387, SEQ ID NO: 389, SEQID NO: 391, SEQ ID NO: 393, SEQ ID NO: 395, SEQ ID NO: 397, SEQ ID NO:399, SEQ ID NO: 401, SEQ ID NO: 403, SEQ ID NO: 405, SEQ ID NO: 407, SEQID NO: 409, SEQ ID NO: 411, SEQ ID NO: 413, SEQ ID NO: 415, SEQ ID NO:417, SEQ ID NO: 419, SEQ ID NO: 421, SEQ ID NO: 423, SEQ ID NO: 425, SEQID NO: 427, SEQ ID NO: 429, SEQ ID NO: 431, SEQ ID NO: 433, SEQ ID NO:435 or SEQ ID NO: 441 in amino acid sequence due to natural variation ormutagenesis, as described in detail herein. Accordingly, the polypeptidecomprise an amino acid sequence which is at least about 35%, 40%, 45%,50%, 55%, 60%, 65% or 70%, preferably at least about 75%, 80%, 85% or90, and more preferably at least about 91%, 92%, 93%, 94% or 95%, andmost preferably at least about 96%, 97%, 98%, 99% or more homologous toan entire amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO:6, SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441.

For the comparison of amino acid sequences the same algorithms asdescribed above or nucleic acid sequences can be used. Results of highquality are reached by using the algorithm of Needleman and Wunsch orSmith and Waterman. Therefore programs based on said algorithms arepreferred. Advantageously the comparisons of sequences can be done withthe program PileUp (J. Mol. Evolution, 25, 351-360, 1987, Higgins etal., CABIOS, 5 1989: 151-153) or preferably with the programs Gap andBestFit, which are respectively based on the algorithms of Needleman andWunsch [J. Mol. Biol. 48; 443-453 (1970)] and Smith and Waterman [Adv.Appl. Math. 2; 482-489 (1981)]. Both programs are part of the GCGsoftware-package [Genetics Computer Group, 575 Science Drive, Madison,Wis., USA 53711 (1991); Altschul et al. (1997) Nucleic Acids Res.25:3389 et seq.]. Therefore preferably the calculations to determine thepercentages of sequence homology are done with the program Gap over thewhole range of the sequences. The following standard adjustments for thecomparison of amino acid sequences were used: gap weight: 8, lengthweight: 2, average match: 2.912, average mismatch: −2.003.

Biologically active portions of an polypeptide of the present inventioninclude peptides comprising amino acid sequences derived from the aminoacid sequence of the polypeptide of the present invention, e.g., theamino acid sequence shown in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441 or the amino acidsequence of a protein homologous thereto, which include fewer aminoacids than a full length protein having the biological activity of theprotein of the invention or the polypeptide of the present invention orthe full length protein which is homologous to a protein having thebiological activity of the protein of the invention or the polypeptideof the present invention depicted herein, and exhibit at least oneactivity of the polypeptide of the present invention, which leads to anincrease of the fine chemical. Typically, biologically (orimmunologically) active portions i.e. peptides, e.g., peptides whichare, for example, 5, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 ormore amino acids in length comprise a domain or motif with at least oneactivity or epitope of a or of the polypeptide of the present invention.Moreover, other biologically active portions, in which other regions ofthe polypeptide are deleted, can be prepared by recombinant techniquesand evaluated for one or more of the activities described herein.

Any mutagenesis strategies for the polypeptide of the present invention,which result in a decreasing biological activity disclosed herein arenot meant to be limiting; variations on these strategies will be readilyapparent to one skilled in the art. Using such strategies, andincorporating the mechanisms disclosed herein, the nucleic acid moleculeand polypeptide of the invention may be utilized to generate plants orparts thereof, expressing mutated nucleic acid molecule and/orpolypeptide molecules of the invention such that the yield, production,and/or efficiency of production of a desired compound such as the finechemical is improved. This desired compound may be any natural productof plants, which includes the final products of biosynthesis pathwaysand intermediates of naturally-occurring metabolic pathways, as well asmolecules which do not naturally occur in the metabolism of said cells,but which are produced by a said cells of the invention.

The invention also provides chimeric or fusion proteins.

As used herein, a “chimeric protein” or “fusion protein” comprises apolypeptide operatively linked to a polypeptide which does not conferabove-mentioned activity, in particular, which does confer an increaseof the fine chemical content in a cell or an organism or a part thereof,if its expression or activity is decreased, e.g. which does not confer abiological activity of the protein of the invention.

In one embodiment, a protein (=“polypeptide”) having the activity toincrease the fine chemical content in the organism, by decreasing itsbiological activity. Said protein refers to a polypeptide having anamino acid sequence corresponding to the polypeptide of the invention,preferably having an amino acid sequence corresponding to thepolypeptides as depicted in SEQ ID NO: 2, SEQ ID NO: 4, SEQ ID NO: 6,SEQ ID NO: 8, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, SEQ ID NO:16, SEQ ID NO: 20, SEQ ID NO: 22, SEQ ID NO: 24, SEQ ID NO: 26, SEQ IDNO: 28, SEQ ID NO. 30, SEQ ID NO: 32; SEQ ID NO: 65, SEQ ID NO: 67, SEQID NO: 69, SEQ ID NO: 71, SEQ ID NO: 73, SEQ ID NO: 75, SEQ ID NO: 77,SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO:87, SEQ ID NO: 89, SEQ ID NO: 91, SEQ ID NO: 93, SEQ ID NO: 95, SEQ IDNO: 97, SEQ ID NO: 99, SEQ ID NO: 101, SEQ ID NO: 103, SEQ ID NO: 105,SEQ ID NO: 107, SEQ ID NO: 109, SEQ ID NO: 111, SEQ ID NO: 113, SEQ IDNO: 115, SEQ ID NO: 117, SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 123,SEQ ID NO: 125, SEQ ID NO: 127, SEQ ID NO: 129, SEQ ID NO: 131, SEQ IDNO: 133, SEQ ID NO: 135, SEQ ID NO: 137, SEQ ID NO: 139, SEQ ID NO: 141,SEQ ID NO: 143, SEQ ID NO: 152, SEQ ID NO: 154, SEQ ID NO: 156, SEQ IDNO: 158, SEQ ID NO: 160, SEQ ID NO: 162, SEQ ID NO: 164, SEQ ID NO: 166,SEQ ID NO: 168, SEQ ID NO: 170, SEQ ID NO: 172, SEQ ID NO: 174, SEQ IDNO: 176, SEQ ID NO: 178, SEQ ID NO: 180, SEQ ID NO: 182, SEQ ID NO: 184,SEQ ID NO: 186, SEQ ID NO: 188, SEQ ID NO: 190, SEQ ID NO: 192, SEQ IDNO: 194, SEQ ID NO: 196, SEQ ID NO: 198, SEQ ID NO: 200, SEQ ID NO: 202,SEQ ID NO: 204, SEQ ID NO: 206, SEQ ID NO: 208, SEQ ID NO: 210, SEQ IDNO: 212, SEQ ID NO: 214, SEQ ID NO: 216, SEQ ID NO: 218, SEQ ID NO: 220,SEQ ID NO: 229, SEQ ID NO:231, SEQ ID NO:233, SEQ ID NO: 235, SEQ ID NO:237, SEQ ID NO: 239, SEQ ID NO: 241, SEQ ID NO: 243, SEQ ID NO: 250, SEQID NO: 252, SEQ ID NO: 254, SEQ ID NO: 256, SEQ ID NO: 258, SEQ ID NO:260, SEQ ID NO: 262, SEQ ID NO: 264, SEQ ID NO: 266, SEQ ID NO: 268, SEQID NO: 270, SEQ ID NO: 272, SEQ ID NO: 283, SEQ ID NO: 285, SEQ ID NO:287, SEQ ID NO: 289, SEQ ID NO: 291, SEQ ID NO: 293, SEQ ID NO: 295, SEQID NO: 297, SEQ ID NO: 299, SEQ ID NO: 301, SEQ ID NO: 303, SEQ ID NO:305, SEQ ID NO: 307, SEQ ID NO: 309, SEQ ID NO: 311, SEQ ID NO: 313, SEQID NO: 315, SEQ ID NO: 317, SEQ ID NO: 319, SEQ ID NO: 321, SEQ ID NO:323, SEQ ID NO: 325, SEQ ID NO: 327, SEQ ID NO: 329, SEQ ID NO: 331, SEQID NO: 333, SEQ ID NO: 335, SEQ ID NO: 337, SEQ ID NO: 339, SEQ ID NO:341, SEQ ID NO: 343, SEQ ID NO: 345, SEQ ID NO: 347, SEQ ID NO: 349, SEQID NO: 351, SEQ ID NO: 353, SEQ ID NO: 355, SEQ ID NO: 357, SEQ ID NO:359, SEQ ID NO: 361, SEQ ID NO: 363, SEQ ID NO: 365, SEQ ID NO: 367, SEQID NO: 369, SEQ ID NO: 371, SEQ ID NO: 373, SEQ ID NO: 375, SEQ ID NO:377, SEQ ID NO: 379, SEQ ID NO: 381, SEQ ID NO: 383, SEQ ID NO: 385, SEQID NO: 387, SEQ ID NO: 389, SEQ ID NO: 391, SEQ ID NO: 393, SEQ ID NO:395, SEQ ID NO: 397, SEQ ID NO: 399, SEQ ID NO: 401, SEQ ID NO: 403, SEQID NO: 405, SEQ ID NO: 407, SEQ ID NO: 409, SEQ ID NO: 411, SEQ ID NO:413, SEQ ID NO: 415, SEQ ID NO: 417, SEQ ID NO: 419, SEQ ID NO: 421, SEQID NO: 423, SEQ ID NO: 425, SEQ ID NO: 427, SEQ ID NO: 429, SEQ ID NO:431, SEQ ID NO: 433, SEQ ID NO: 435 or SEQ ID NO: 441, whereas a“non-protein of the invention polypeptide” refers to a polypeptidehaving an amino acid sequence corresponding to a protein which is notsubstantially homologous to a polypeptide of the invention, preferablywhich is not substantially homologous to a polypeptide having thebiological activity of the protein of the invention, e.g., a proteinwhich does not confer the activity described herein and which is derivedfrom the same or a different organism.

Within the fusion protein, the term “operatively linked” is intended toindicate that the polypeptide of the invention and the other polypeptideor part thereof are fused to each other so that both sequences fulfilthe proposed function addicted to the sequence used. The otherpolypeptide can be fused to the N-terminus or C-terminus of thepolypeptide of the invention. For example, in one embodiment the fusionprotein is a GST-LMRP fusion protein in which the sequences of thepolypeptide of the invention are fused to the C-terminus of the GSTsequences. Such fusion proteins can facilitate the purification ofrecombinant polypeptides of the invention.

In another embodiment, the fusion protein is a polypeptide of theinvention containing a heterologous signal sequence at its N-terminus.In certain host cells (e.g., mammalian host cells), expression and/orsecretion of the protein of the invention decreased in its biologicalactivity can be increased through use of a heterologous signal sequence.Such sequences are for example targeting sequences. As already mentionedabove, targeting sequences, are required for targeting the gene productinto specific cell compartment (for a review, see Kermode, Crit. Rev.Plant Sci. 15, 4 (1996) 285-423 and references cited therein), forexample into the vacuole, the nucleus, all types of plastids, such asamyloplasts, chloroplasts, chromoplasts, the extracellular space, themitochondria, the endoplasmic reticulum, elaioplasts, peroxisomes,glycosomes, and other compartments of cells or extracellular. Sequences,which must be mentioned in this context, are, in particular, thesignal-peptide- or transit-peptide-encoding sequences, which are knownper se. For example, plastid-transit-peptide-encoding sequences enablethe targeting of the expression product into the plastids of a plantcell. Targeting sequences are also known for eukaryotic and to a lowerextent for prokaryotic organisms and can advantageously be operablelinked with the nucleic acid molecule of the present invention toachieve an expression in one of said compartments or extracellular.

Preferably, a chimeric or fusion protein of the invention is produced bystandard recombinant DNA techniques. For example, DNA fragments codingfor the different polypeptide sequences are ligated together in-frame inaccordance with conventional techniques, for example by employingblunt-ended or stagger-ended termini for ligation, restriction enzymedigestion to provide for appropriate termini, filling-in of cohesiveends as appropriate, alkaline phosphatase treatment to avoid undesirablejoining, and enzymatic ligation. The fusion gene can be synthesized byconventional techniques including automated DNA synthesizers.Alternatively, PCR amplification of gene fragments can be carried outusing anchor primers, which give rise to complementary overhangs betweentwo consecutive gene fragments which can subsequently be annealed andreamplified to generate a chimeric gene sequence (see, for example,Current Protocols in Molecular Biology, eds. Ausubel et al. John Wiley &Sons: 1992). Moreover, many expression vectors are commerciallyavailable that already encode a fusion moiety (e.g., a GST polypeptide).The nucleic acid molecule of the invention can be cloned into such anexpression vector such that the fusion moiety is linked in-frame to theencoded protein.

Furthermore, folding simulations and computer redesign of structuralmotifs of the protein of the invention can be performed usingappropriate computer programs (Olszewski, Proteins 25 (1996), 286-299;Hoffman, Comput. Appl. Biosci. 11 (1995), 675-679). Computer modeling ofprotein folding can be used for the conformational and energeticanalysis of detailed peptide and protein models (Monge, J. Mol. Biol.247 (1995), 995-1012; Renouf, Adv. Exp. Med. Biol. 376 (1995), 37-45).The appropriate programs can be used for the identification ofinteractive sites of the polypeptide of the invention and its substratesor binding factors or other interacting proteins by computer assistantsearches for complementary peptide sequences (Fassina, Immunomethods(1994), 114-120). Further appropriate computer systems for the design ofprotein and peptides are described in the prior art, for example inBerry, Biochem. Soc. Trans. 22 (1994), 1033-1036; Wodak, Ann. N.Y. Acad.Sci. 501 (1987), 1-13; Pabo, Biochemistry 25 (1986), 5987-5991 Theresults obtained from the above-described computer analysis can be usedfor, e.g., the preparation of peptidomimetics of the protein of theinvention or fragments thereof. Such pseudopeptide analogues of the,natural amino acid sequence of the protein may very efficiently mimicthe parent protein (Benkirane, J. Biol. Chem. 271 (1996), 33218-33224).For example, incorporation of easily available achiral Q-amino acidresidues into a protein of the invention or a fragment thereof resultsin the substitution of amide bonds by polymethylene units of analiphatic chain, thereby providing a convenient strategy forconstructing a peptidomimetic (Banerjee, Biopolymers 39 (1996),769-777).

Superactive peptidomimetic analogues of small peptide hormones in othersystems are described in the prior art (Zhang, Biochem. Biophys. Res.Commun. 224 (1996), 327-331). Appropriate peptidomimetics of the proteinof the present invention can also be identified by the synthesis ofpeptidomimetic combinatorial libraries through successive amidealkylation and testing the resulting compounds, e.g., for their bindingand immunological properties. Methods for the generation and use ofpeptidomimetic combinatorial libraries are described in the prior art,for example in Ostresh, Methods in Enzymology 267 (1996), 220-234 andDorner, Bioorg. Med. Chem. 4 (1996), 709-715.

Furthermore, a three-dimensional and/or crystallographic structure ofthe protein of the invention can be used for the design ofpeptidomimetic inhibitors of the biological activity of the protein ofthe invention (Rose, Biochemistry 35 (1996), 12933-12944; Rutenber,Bioorg. Med. Chem. 4 (1996), 1545-1558).

Furthermore, a three-dimensional and/or crystallographic structure ofthe protein of the invention and the identification of interactive sitesthe polypeptide of the invention and its substrates or binding factorscan be used for design of mutants with modulated binding or turn overactivities. For example, the active center of the polypeptide of thepresent invention can be modelled and amino acid residues participatingin the catalytic reaction can be modulated to increase or decrease thebinding of the substrate to inactivate the polypeptide. Theidentification of the active center and the amino acids involved in thecatalytic reaction facilitates the screening for mutants having anincreased activity.

The sequence shown in SEQ ID NO: 2 has been described under itsAccession Number At2g25320 as a hypothetical proteins, which has a weaksimilarity to the ubiquitin-specific protease 12. However, an activityof the protein disclosed under the Accession Number At2g25320 has notbeen proven experimentally as yet. The protein depicted in SEQ ID NO: 8is deposited in Genebank under the Accession Number At3g07610. Theprotein contains a protein domain, which is similar to the transcriptionfactor jumonji domain. The activity of the protein is still unknown. Theprotein depicted in SEQ ID NO: 20 and deposited at Genebank under theAccession Number At3g15600 is similar to an En/Spm-like transposonprotein. The function and activity of the protein is unknown. Theprotein depicted in SEQ ID NO: 65 and deposited at the Genebank underthe Accession Number At1g14490 is possibly a DNA-binding protein.Accession Number At3g30570, which has SEQ ID NO: 152 is a putativeserine/thronine protein kinase. The protein depicted in SEQ ID NO: 229and deposited at the Genebank under the Accession Number At2g21290 hasan unknown function. Furthermore the protein depicted in SEQ ID NO: 283and deposited at the Genebank under the Accession Number At3g14230 is atranscription factor EREBP-like protein.

One embodiment of the invention also relates to an antibody, which bindsspecifically to the polypeptide according to the invention or parts,i.e. specific fragments or epitopes of such a protein.

The antibodies of the invention can be used to identify and isolate thepolypeptide according to the invention and encoding genes in anyorganism, preferably plants, prepared in plants described herein. Suchantibodies can also be expressed in the suitable host organisms therebyreducing the biological activity of the genes of the invention bybinding to their protein products leading for example to a stericinterference with their biological activity. These antibodies can bemonoclonal antibodies, polyclonal antibodies or synthetic antibodies aswell as fragments of antibodies, such as Fab, Fv or scFv fragments etc.Monoclonal antibodies can be prepared, for example, by the techniques asoriginally described in Köhler and Milstein, Nature 256 (1975), 495, andGalfr6, Meth. Enzymol. 73 (1981), 3, which comprise the fusion of mousemyeloma cells to spleen cells derived from immunized mammals.

Furthermore, antibodies or fragments thereof to the aforementionedpeptides can be obtained by using methods, which are described, e.g., inHarlow and Lane “Antibodies, A Laboratory Manual”, CSH Press, ColdSpring Harbor, 1988. These antibodies can be used, for example, for theimmunoprecipitation and immunolocalization of proteins according to theinvention as well as for the monitoring of the synthesis of suchproteins, for example, in recombinant organisms, and for theidentification of compounds interacting with the protein according tothe invention. For example, surface plasmon resonance as employed in theBIAcore system can be used to increase the efficiency of phageantibodies selections, yielding a high increment of affinity from asingle library of phage antibodies, which bind to an epitope of theprotein of the invention (Schier, Human Antibodies Hybridomas 7 (1996),97-105; Malmborg, J. Immunol. Methods 183 (1995), 7-13). In many cases,the binding phenomena of antibodies to antigens is equivalent to otherligand/anti-ligand binding.

A further embodiment of the invention also relates to a method for thegeneration of a transgenic host cell, e.g. a eukaryotic or prokaryotichost or host cell, preferably a transgenic microorganism, a transgenicplant cell or a transgenic plant tissue or a transgenic plant, whichcomprises introducing, into the plant, the plant cell or the planttissue, the nucleic acid construct according to the invention, thevector according to the invention, or the nucleic acid moleculeaccording to the invention.

A further embodiment of the invention also relates to a method for thetransient generation of a host or host cell, eukaryotic or prokaryoticcell, preferably a transgenic microorganism, a transgenic plant cell ora transgenic plant tissue or a transgenic plant, which comprisesintroducing, into the plant, the plant cell or the plant tissue, thenucleic acid construct according to the invention, the vector accordingto the invention, the nucleic acid molecule characterized herein asbeing contained in the nucleic acid construct of the invention or thenucleic acid molecule according to the invention, whereby the introducednucleic acid molecules, nucleic acid construct and/or vector is notintegrated into the genome of the host or host cell. Therefore thetransformants are not stable during the propagation of the host inrespect of the introduced nucleic acid molecules, nucleic acid constructand/or vector.

In the process according to the invention, transgenic organisms are alsoto be understood as meaning—if they take the form of plants—plant cells,plant tissues, plant organs such as root, shoot, stem, seed, flower,tuber or leaf, or intact plants which are grown for the production ofamino acids.

Growing is to be understood as meaning for example culturing thetransgenic plant cells, plant tissue or plant organs on or in a nutrientmedium or the intact plant on or in a substrate, for example inhydroponic culture, potting compost or on a field soil.

In a further advantageous embodiment of the process, the nucleic acidmolecules can be expressed in single-celled plant cells (such as algae),see Falciatore et al., 1999, Marine Biotechnology 1 (3): 239-251 andreferences cited therein, and plant cells from higher plants (forexample spermatophytes such as crops). Examples of plant expressionvectors encompass those which are described in detail herein or in:Becker, D. [(1992) Plant Mol. Biol. 20:1195-1197] and Bevan, M. W.[(1984), Nucl. Acids Res. 12:8711-8721; Vectors for Gene Transfer inHigher Plants; in: Transgenic Plants, Vol. 1, Engineering andUtilization, Ed.: Kung and R. Wu, Academic Press, 1993, pp. 15-38]. Anoverview of binary vectors and their use is also found in Hellens, R.[(2000), Trends in Plant Science, Vol. 5 No. 10, 446-451.

Vector DNA can be introduced into prokaryotic or eukaryotic cells viaconventional transformation or transfection techniques. The terms“transformation” and “transfection” include conjugation and transductionand, as used in the present context, are intended to encompass amultiplicity of prior-art methods for introducing foreign nucleic acidmolecules (for example DNA) into a host cell, including calciumphosphate coprecipitation or calcium chloride coprecipitation,DEAE-dextran-mediated transfection, PEG-mediated transfection,lipofection, natural competence, chemically mediated transfer,electroporation or particle bombardment. Suitable methods for thetransformation or transfection of host cells, including plant cells, canbe found in Sambrook et al. (Molecular Cloning: A Laboratory Manual.,2^(nd) Ed., Cold Spring Harbor Laboratory, Cold Spring Harbor LaboratoryPress, Cold Spring Harbor, N.Y., 1989) and in other laboratory handbookssuch as Methods in Molecular Biology, 1995, Vol. 44, Agrobacteriumprotocols, Ed.: Gartland and Davey, Humana Press, Totowa, N.J.

The above-described methods for the transformation and regeneration ofplants from plant tissues or plant cells are exploited for transient orstable transformation of plants. Suitable methods are the transformationof protoplasts by polyethylene-glycol-induced DNA uptake, the biolisticmethod with the gene gun—known as the particle bombardment method—,electroporation, the incubation of dry embryos in DNA-containingsolution, microinjection and the Agrobacterium-mediated gene transfer.The abovementioned methods are described for example in B. Jenes,Techniques for Gene Transfer, in: Transgenic Plants, Vol. 1, Engineeringand Utilization, edited by S. D. Kung and R. Wu, Academic Press (1993)128-143 and in Potrykus Annu. Rev. Plant Physiol. Plant Molec. Biol. 42(1991) 205-225. The construct to be expressed is preferably cloned intoa vector, which is suitable for transforming Agrobacterium tumefaciens,for example pBin19 (Bevan, Nucl. Acids Res. 12 (1984) 8711).Agrobacteria transformed with such a vector can then be used in theknown manner for the transformation of plants, in particular cropplants, such as, for example, tobacco plants, for example by bathingscarified leaves or leaf segments in an agrobacterial solution andsubsequently culturing them in suitable media. The transformation ofplants with Agrobacterium tumefaciens is described for example by Höfgenand Willmitzer in Nucl. Acid Res. (1988) 16, 9877 or known from, interalia, F. F. White, Vectors for Gene Transfer in Higher Plants; inTransgenic Plants, Vol. 1, Engineering and Utilization, edited by S. D.Kung and R. Wu, Academic Press, 1993, pp. 15-38.

To select for the successful transfer of the nucleic acid molecule,vector or nucleic acid construct of the invention according to theinvention into a host organism, it is advantageous to use marker genesas have already been described above in detail. It is known of thestable or transient integration of nucleic acids into plant cells thatonly a minority of the cells takes up the foreign DNA and, if desired,integrates it into its genome, depending on the expression vector usedand the transfection technique used. To identify and select theseintegrants, a gene encoding for a selectable marker (as described above,for example resistance to antibiotics) is usually introduced into thehost cells together with the gene of interest. Preferred selectablemarkers in plants comprise those, which confer resistance to anherbicide such as glyphosate or gluphosinate. Other suitable markersare, for example, markers, which encode genes involved in biosyntheticpathways of, for example, sugars or amino acids, such asβ-galactosidase, ura3 or ilv2. Markers, which encode genes such asluciferase, gfp or other fluorescence genes, are likewise suitable.These markers and the aforementioned markers can be used in mutants inwhom these genes are not functional since, for example, they have beendeleted by conventional methods. Furthermore, nucleic acid molecules,which encode a selectable marker, can be introduced into a host cell onthe same vector as those, which encode the polypeptides of the inventionor used in the process or else in a separate vector. Cells which havebeen transfected stably with the nucleic acid introduced can beidentified for example by selection (for example, cells which haveintegrated the selectable marker survive whereas the other cells die).

Since the marker genes, as a rule specifically the gene for resistanceto antibiotics and herbicides, are no longer required or are undesiredin the transgenic host cell once the nucleic acids have been introducedsuccessfully, the process according to the invention for introducing thenucleic acids advantageously employs techniques which enable theremoval, or excision, of these marker genes. One such a method is whatis known as cotransformation. The cotransformation method employs twovectors simultaneously for the transformation, one vector bearing thenucleic acid according to the invention and a second bearing the markergene(s). A large proportion of transformants receives or, in the case ofplants, comprises (up to 40% of the transformants and above), bothvectors. The marker genes can subsequently be removed from thetransformed plant by performing crosses. In another method, marker genesintegrated into a transposon are used for the transformation togetherwith desired nucleic acid (known as the Ac/Ds technology). In some cases(approx. 10%), the transposon jumps out of the genome of the host cellonce transformation has taken place successfully and is lost. In afurther number of cases, the transposon jumps to a different location.In these cases, the marker gene must be eliminated by performingcrosses. In microbiology, techniques were developed which make possible,or facilitate, the detection of such events. A further advantageousmethod relies on what are known as recombination systems, whoseadvantage is that elimination by crossing can be dispensed with. Thebest-known system of this type is what is known as the Cre/lox system.Cre1 is a recombinase, which removes the sequences located between theloxP sequence. If the marker gene is integrated between the loxPsequence, it is removed, once transformation has taken placesuccessfully, by expression of the recombinase. Further recombinationsystems are the HIN/HIX, FLP/FRT and REP/STB system (Tribble et al., J.Biol. Chem., 275, 2000: 22255-22267; Velmurugan et al., J. Cell Biol.,149, 2000: 553-566). A site-specific integration into the plant genomeof the nucleic acid sequences according to the invention is possible.Naturally, these methods can also be applied to microorganisms such asyeast, fungi or bacteria.

Agrobacteria transformed with an expression vector according to theinvention may also be used in the manner known per se for thetransformation of plants such as experimental plants like Arabidopsis orcrop plants, such as, for example, cereals, maize, oats, rye, barley,wheat, soya, rice, cotton, sugarbeet, canola, sunflower, flax, hemp,potato, tobacco, tomato, carrot, bell peppers, oilseed rape, tapioca,cassava, arrow root, tagetes, alfalfa, lettuce and the various tree,nut, and grapevine species, in particular oil-containing crop plantssuch as soya, peanut, castor-oil plant, sunflower, maize, cotton, flax,oilseed rape, coconut, oil palm, safflower (Carthamus tinctorius) orcocoa beans, for example by bathing scarified leaves or leaf segments inan agrobacterial solution and subsequently growing them in suitablemedia.

In addition to the transformation of somatic cells, which then has to beregenerated into intact plants, it is also possible to transform thecells of plant meristems and in particular those cells which developinto gametes. In this case, the transformed gametes follow the naturalplant development, giving rise to transgenic plants. Thus, for example,seeds of Arabidopsis are treated with agrobacteria and seeds areobtained from the developing plants of which a certain proportion istransformed and thus transgenic (Feldman, K A and Marks M D (1987). MolGen Genet 208:274-289; Feldmann K (1992). In: C Koncz, N-H Chua and JShell, eds, Methods in Arabidopsis Research. Word Scientific, Singapore,pp. 274-289). Alternative methods are based on the repeated removal ofthe influorescences and incubation of the excision site in the center ofthe rosette with transformed agrobacteria, whereby transformed seeds canlikewise be obtained at a later point in time (Chang (1994). Plant J. 5:551-558; Katavic (1994). Mol Gen Genet, 245: 363-370). However, anespecially effective method is the vacuum infiltration method with itsmodifications such as the “floral dip” method. In the case of vacuuminfiltration of Arabidopsis, intact plants under reduced pressure aretreated with an agrobacterial suspension (Bechthold, N (1993). C R AcadSci Paris Life Sci, 316: 1194-1199), while in the case of the “floraldip” method the developing floral tissue is incubated briefly with asurfactant-treated agrobacterial suspension (Clough, S J und Bent, A F(1998). The Plant J. 16, 735-743). A certain proportion of transgenicseeds are harvested in both cases, and these seeds can be distinguishedfrom nontransgenic seeds by growing under the above-described selectiveconditions.

The genetically modified plant cells can be regenerated via all methodswith which the skilled worker is familiar. Suitable methods can be foundin the abovementioned publications by S. D. Kung and R. Wu, Potrykus orHöfgen and Willmitzer.

Accordingly, the present invention thus also relates to a plant cellcomprising the nucleic acid construct according to the invention, thenucleic acid molecule according to the invention or the vector accordingto the invention.

Accordingly the present invention relates to any cell transgenic for anynucleic acid characterized as part of the invention, e.g. conferring theincrease of the fine chemical in a cell or an organism or a partthereof, e.g. the nucleic acid molecule of the invention, the nucleicacid construct of the invention, the antisense molecule of theinvention, the vector of the invention or a nucleic acid moleculeencoding the polypeptide of the invention, e.g. encoding a polypeptidehaving biological activity of the protein of the invention. Due to theabovementioned activity the fine chemical content in a cell or anorganism is increased. For example, due to modulation or manipulation,the cellular activity is increased y, e.g. due to a decreased expressionor decreased specific activity of the subject matters of the inventionin a cell or an organism or a part thereof.

A naturally occurring expression cassette—for example the naturallyoccurring combination of the promoter of the protein of the inventionwith the corresponding gene, which codes for the protein of theinvention—becomes a transgenic expression cassette when it is modifiedby non-natural, synthetic “artificial” methods such as, for example,mutagenization. Such methods have been described (U.S. Pat. No.5,565,350; WO 00/15815; also see above).

Further, the plant cell, plant tissue or plant can also be transformedsuch that further enzymes and proteins are (over)expressed whichexpression supports an increase of the fine chemical.

The term “transgenic plants” used in accordance with the inventionrefers to the progeny of a transgenic plant, for example the T₁, T₂, T₃and subsequent plant generations or the BC₁, BC₂, BC₃ and subsequentplant generations. Thus, the transgenic plants according to theinvention can be raised and selfed or crossed with other individuals inorder to obtain further transgenic plants according to the invention.Transgenic plants may also be obtained by propagating transgenic plantcells vegetatively. The present invention also relates to transgenicplant material, which can be derived from a transgenic plant populationaccording to the invention. Such material includes plant cells andcertain tissues, organs and parts of plants in all their manifestations,such as seeds, leaves, anthers, fibers, tubers, roots, root hairs,stems, embryo, calli, cotelydons, petioles, harvested material, planttissue, reproductive tissue and cell cultures, which are derived fromthe actual transgenic plant and/or can be used for bringing about thetransgenic plant.

Any transformed plant obtained according to the invention can be used ina conventional breeding scheme or in in vitro plant propagation toproduce more transformed plants with the same characteristics and/or canbe used to introduce the same characteristic in other varieties of thesame or related species. Such plants are also part of the invention.Seeds obtained from the transformed plants genetically also contain thesame characteristic and are part of the invention. As mentioned before,the present invention is in principle applicable to any plant and cropthat can be transformed with any of the transformation method known tothose skilled in the art.

In an especially preferred embodiment, the organism, the host cell,plant cell, plant, microorganism or plant tissue according to theinvention is transgenic.

Accordingly, the invention therefore relates to transgenic organismstransformed with at least one nucleic acid molecule, nucleic acidconstruct or vector according to the invention, and to cells, cellcultures, tissues, parts—such as, for example, in the case of plantorganisms, plant tissue, for example leaves, roots and the like—orpropagation material derived from such organisms, or intact plants. Theterms “recombinant (host)” and “transgenic (host)” are usedinterchangeably in this context. Naturally, these terms refer not onlyto the host organism or target cell in question, but also to theprogeny, or potential progeny, of these organisms or cells. Sincecertain modifications may occur in subsequent generations owing tomutation or environmental effects, such progeny is not necessarilyidentical with the parental cell, but still comes within the scope ofthe term as used herein.

Suitable organisms for the process according to the invention or ashosts are all these eukaryotic or prokaryotic organisms, which arecapable of synthesizing the fine chemical. The organisms used as hostsare microorganisms, such as bacteria, fungi, yeasts or algae, non-humananimals, or plants, such as dictotyledonous or monocotyledonous plants.

In principle all plants can be used as host organism, especially theplants mentioned above as source organism. Preferred transgenic plantsare, for example, selected from the families Aceraceae, Anacardiaceae,Apiaceae, Asteraceae, Brassicaceae, Cactaceae, Cucurbitaceae,Euphorbiaceae, Fabaceae, Malvaceae, Nymphaeaceae, Papaveraceae,Rosaceae, Salicaceae, Solanaceae, Arecaceae, Bromeliaceae, Cyperaceae,Iridaceae, Liliaceae, Orchidaceae, Gentianaceae, Labiaceae,Magnoliaceae, Ranunculaceae, Carifolaceae, Rubiaceae, Scrophulariaceae,Caryophyllaceae, Ericaceae, Polygonaceae, Violaceae, Juncaceae orPoaceae and preferably from a plant selected from the group of thefamilies Apiaceae, Asteraceae, Brassicaceae, Cucurbitaceae, Fabaceae,Papaveraceae, Rosaceae, Solanaceae, Liliaceae or Poaceae. Preferred arecrop plants such as plants advantageously selected from the group of thegenus peanut, oilseed rape, canola, sunflower, safflower, olive, sesame,hazelnut, almond, avocado, bay, pumpkin/squash, linseed, soya,pistachio, borage, maize, wheat, rye, oats, sorghum and millet,triticale, rice, barley, cassava, potato, sugarbeet, egg plant, alfalfa,and perennial grasses and forage plants, oil palm, vegetables(brassicas, root vegetables, tuber vegetables, pod vegetables, fruitingvegetables, onion vegetables, leafy vegetables and stem vegetables),buckwheat, Jerusalem artichoke, broad bean, vetches, lentil, dwarf bean,lupin, clover and Lucerne for mentioning only some of them.

Preferred plant cells, plant organs, plant tissues or parts of plantsoriginate from the under source organism mentioned plant families,preferably from the abovementioned plant genus, more preferred fromabovementioned plants species.

Transgenic plants comprising the fine chemical synthesized in theprocess according to the invention can be marketed directly withoutisolation of the compounds synthesized. In the process according to theinvention, plants are understood as meaning all plant parts, plantorgans such as leaf, stalk, root, tubers or seeds or propagationmaterial or harvested material or the intact plant. In this context, theseed encompasses all parts of the seed such as the seed coats, epidermalcells, seed cells, endosperm or embryonic tissue. The fine chemicalproduced in the process according to the invention may, however, also beisolated from the plant in the form of the free compound or bound toother components of the plant. The fine chemical produced by thisprocess can be harvested by harvesting the organisms either from theculture in which they grow or from the field. This can be done viaexpressing, grinding and/or extraction, salt precipitation and/orion-exchange chromatography of the plant parts, preferably the plantseeds, plant fruits, plant tubers and the like.

In a further embodiment, the present invention relates to a process forthe generation of a microorganism, comprising the introduction, into themicroorganism or parts thereof, of the nucleic acid construct of theinvention, or the vector of the invention or the nucleic acid moleculeof the invention.

In another embodiment, the present invention relates also to atransgenic microorganism comprising the nucleic acid molecule of theinvention, the nucleic acid construct of the invention or the vector asof the invention. Appropriate microorganisms have been described hereinbefore under source organism, preferred are in particular aforementionedstrains suitable for the production of fine chemicals.

Accordingly, the present invention relates also to a process accordingto the present invention whereby the fine chemical produced in theinventive process is isolated.

In this manner, more than 50% by weight, advantageously more than 60% byweight, preferably more than 70% by weight, especially preferably morethan 80% by weight, very especially preferably more than 90% by weight,of the fine chemical produced in the process can be isolated. Theresulting fine chemical can, if appropriate, subsequently be furtherpurified, if desired mixed with other active ingredients such asvitamins, amino acids, carbohydrates, antibiotics and the like, and, ifappropriate, formulated.

The amino acids obtained in the process are suitable as startingmaterial for the synthesis of further products of value. For example,they can be used in combination with each other or alone for theproduction of pharmaceuticals, foodstuffs, animal feeds or cosmetics.Accordingly, the present invention relates a method for the productionof pharmaceuticals, food stuff, animal feeds, nutrients or cosmeticscomprising the steps of the process according to the invention,including the isolation of the amino acid composition produced or theproduced methionine if desired and formulating the product with apharmaceutical acceptable carrier or formulating the product in a formacceptable for an application in agriculture. A further embodimentaccording to the invention is the use of the amino acids produced in theprocess or of the transgenic organisms in animal feeds, foodstuffs,medicines, food supplements, cosmetics or pharmaceuticals. Yet anotherembodiment of the invention is a composition comprising the protein ofthe invention, the nucleic acid molecule of the invention, thepolypeptide of the invention, the nucleic acid construct or the vectorof the invention, the antagonist of the invention, the antibody of theinvention and optionally a agricultural acceptable carrier.

In principle all microorganisms can be used as host organism especiallythe ones mentioned under source organism above. It is advantageous touse in the process of the invention transgenic microorganisms such asfungi such as the genus Claviceps or Aspergillus or Gram-positivebacteria such as the genera Bacillus, Corynebacterium, Micrococcus,Brevibacterium, Rhodococcus, Nocardia, Caseobacter or Arthrobacter orGram-negative bacteria such as the genera Escherichia, Flavobacterium orSalmonella or yeasts such as the genera Rhodotorula, Hansenula orCandida. Particularly advantageous organisms are selected from the groupof genera Corynebacterium, Brevibacterium, Escherichia, Bacillus,Rhodotorula, Hansenula, Candida, Claviceps or Flavobacterium. It is veryparticularly advantageous to use in the process of the inventionmicroorganisms selected from the group of genera and species consistingof Hansenula anomala, Candida utilis, Claviceps purpurea, Bacilluscirculans, Bacillus subtilis, Bacillus sp., Brevibacterium albidum,Brevibacterium album, Brevibacterium cerinum, Brevibacterium flavum,Brevibacterium glutamigenes, Brevibacterium iodinum, Brevibacteriumketoglutamicum, Brevibacterium lactofermentum, Brevibacterium linens,Brevibacterium roseum, Brevibacterium saccharolyticum, Brevibacteriumsp., Corynebacterium acetoaciclophilum, Corynebacterium acetoglutamicum,Corynebacterium ammoniagenes, Corynebacterium glutamicum (=Micrococcusglutamicum), Corynebacterium melassecola, Corynebacterium sp. orEscherichia coli, specifically Escherichia coli K12 and its describedstrains.

The process of the invention is, when the host organisms aremicroorganisms, advantageously carried out at a temperature between 0°C. and 95° C., preferably between 10° C. and 85° C., particularlypreferably between 15° C. and 75° C., very particularly preferablybetween 15° C. and 45° C. The pH is advantageously kept at between pH 4and 12, preferably between pH 6 and 9, particularly preferably betweenpH 7 and 8, during this. The process of the invention can be operatedbatchwise, semibatchwise or continuously. A summary of known cultivationmethods is to be found in the textbook by Chmiel (Bioprozeβtechnik 1.Einführung in die Bioverfahrenstechnik (Gustav Fischer Verlag,Stuttgart, 1991)) or in the textbook by Storhas (Bioreaktoren undperiphere Einrichtungen (Vieweg Verlag, Braunschweig/Wiesbaden, 1994)).The culture medium to be used must meet the requirements of therespective strains in a suitable manner. Descriptions of culture mediafor various microorganisms are present in the handbook “Manual ofMethods for General Bacteriology” of the American Society forBacteriology (Washington D.C., USA, 1981). These media, which can beemployed according to the invention include, as described above, usuallyone or more carbon sources, nitrogen sources, inorganic salts, vitaminsand/or trace elements. Preferred carbon sources are sugars such asmono-, di- or polysaccharides. Examples of very good carbon sources areglucose, fructose, mannose, galactose, ribose, sorbose, ribulose,lactose, maltose, sucrose, raffinose, starch or cellulose. Sugars canalso be added to the media via complex compounds such as molasses, orother byproducts of sugar refining. It may also be advantageous to addmixtures of various carbon sources. Other possible carbon sources areoils and fats such as, for example, soybean oil, sunflower oil, peanutoil and/or coconut fat, fatty acids such as, for example, palmitic acid,stearic acid and/or linoleic acid, alcohols and/or polyalcohols such as,for example, glycerol, methanol and/or ethanol and/or organic acids suchas, for example, acetic acid and/or lactic acid. Nitrogen sources areusually organic or inorganic nitrogen compounds or materials, whichcontain these compounds. Examples of nitrogen sources include ammonia inliquid or gaseous form or ammonium salts such as ammonium sulfate,ammonium chloride, ammonium phosphate, ammonium carbonate or ammoniumnitrate, nitrates, urea, amino acids or complex nitrogen sources such ascorn steep liquor, soybean meal, soybean protein, yeast extract, meatextract and others. The nitrogen sources may be used singly or as amixture. Inorganic salt compounds, which may be present in the mediainclude the chloride, phosphorus or sulfate salts of calcium, magnesium,sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron.For preparing sulfur-containing fine chemicals, in particularmethionine, it is possible to use as sulfur source inorganicsulfur-containing compounds such as, for example, sulfates, sulfites,dithionites, tetrathionates, thiosulfates, sulfides or else organicsulfur compounds such as mercaptans and thiols. It is possible to use asphosphorus source phosphoric acid, potassium dihydrogenphosphate ordipotassium hydrogenphosphate or the corresponding sodium-containingsalts. Chelating agents can be added to the medium in order to keep themetal ions in solution. Particularly suitable chelating agents includedihydroxyphenols such as catechol or protocatechuate, or organic acidssuch as citric acid. The fermentation media employed according to theinvention for cultivating microorganisms normally also contain othergrowth factors such as vitamins or growth promoters, which include, forexample, biotin, riboflavin, thiamine, folic acid, nicotinic acid,pantothenate and pyridoxine. Growth factors and salts are often derivedfrom complex media components such as yeast extract, molasses, cornsteep liquor and the like. Suitable precursors can moreover be added tothe culture medium. The exact composition of the media compounds dependsgreatly on the particular experiment and is chosen individually for eachspecific case. Information about media optimization is obtainable fromthe textbook “Applied Microbiol. Physiology, A Practical Approach”(editors P. M. Rhodes, P. F. Stanbury, IRL Press (1997) pp. 53-73, ISBN0 19 963577 3). Growth media can also be purchased from commercialsuppliers such as Standard 1 (Merck) or BHI (Brain heart infusion,DIFCO) and the like. All media components are sterilized either by heat(1.5 bar and 121° C. for 20 min) or by sterilizing filtration. Thecomponents can be sterilized either together or, if necessary,separately. All media components can be present at the start of thecultivation or optionally be added continuously or batchwise. Thetemperature of the culture is normally between 15° C. and 45° C.,preferably at 25° C. to 40° C., and can be kept constant or changedduring the experiment. The pH of the medium should be in the range from5 to 8.5, preferably around 7. The pH for the cultivation can becontrolled during the cultivation by adding basic compounds such assodium hydroxide, potassium hydroxide, ammonia or aqueous ammonia oracidic compounds such as phosphoric acid or sulfuric acid. Foaming canbe controlled by employing antifoams such as, for example, fatty acidpolyglycol esters. The stability of plasmids can be maintained by addingto the medium suitable substances having a selective effect, for exampleantibiotics. Aerobic conditions are maintained by introducing oxygen oroxygen-containing gas mixtures such as, for example, ambient air intothe culture. The temperature of the culture is normally from 20° C. to45° C. and preferably from 25° C. to 40° C. The culture is continueduntil formation of the desired product is at a maximum. This aim isnormally achieved within 10 hours to 160 hours. The fermentation brothsobtained in this way, containing in particular L-methionine, L-threonineand/or L-lysine, normally have a dry matter content of from 7.5 to 25%by weight. Sugar-limited fermentation is additionally advantageous, atleast at the end, but especially over at least 30% of the fermentationtime. This means that the concentration of utilizable sugar in thefermentation medium is kept at, or reduced to, ≧0 to 3g/l during thistime. The fermentation broth is then processed further. Depending onrequirements, the biomass can be removed entirely or partly byseparation methods, such as, for example, centrifugation, filtration,decantation or a combination of these methods, from the fermentationbroth or left completely in it. The fermentation broth can then bethickened or concentrated by known methods, such as, for example, withthe aid of a rotary evaporator, thin-film evaporator, falling filmevaporator, by reverse osmosis or by nanofiltration. This concentratedfermentation broth can then be worked up by freeze-drying, spray drying,spray granulation or by other processes.

However, it is also possible to purify the amino acid produced further.For this purpose, the product-containing composition is subjected to achromatography on a suitable resin, in which case the desired product orthe impurities are retained wholly or partly on the chromatographyresin. These chromatography steps can be repeated if necessary, usingthe same or different chromatography resins. The skilled worker isfamiliar with the choice of suitable chromatography resins and theirmost effective use. The purified product can be concentrated byfiltration or ultrafiltration and stored at a temperature at which thestability of the product is a maximum.

The identity and purity of the isolated compound(s) can be determined byprior art techniques. These include high performance liquidchromatography (HPLC), spectroscopic methods, mass spectrometry (MS),staining methods, thin-layer chromatography, NIRS, enzyme assay ormicrobiological assays. These analytical methods are summarized in:Patek et al. (1994) Appl. Environ. Microbiol. 60:133-140; Malakhova etal. (1996) Biotekhnologiya 11 27-32; and Schmidt et al. (1998)Bioprocess Engineer. 19:67-70. Ulmann's Encyclopedia of IndustrialChemistry (1996) Vol. A27, VCH: Weinheim, pp. 89-90, pp. 521-540, pp.540-547, pp. 559-566, 575-581 and pp. 581-587; Michal, G (1999)Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology,John Wiley and Sons; Fallon, A. et al. (1987) Applications of HPLC inBiochemistry in: Laboratory Techniques in Biochemistry and MolecularBiology, Vol. 17.

In yet another aspect, the invention also relates to harvestable partsand to propagation material of the transgenic plants according to theinvention which either contain transgenic plant cells expressing anucleic acid molecule according to the invention or which contains cellswhich show an reduced, decreased or deleted cellular activity of thepolypeptide of the invention, e.g. a decreased expression level or loweractivity of the described protein.

Harvestable parts can be in principle any useful parts of a plant, forexample, flowers, pollen, seedlings, tubers, leaves, stems, fruit,seeds, roots etc. Propagation material includes, for example, seeds,fruits, cuttings, seedlings, tubers, rootstocks etc. Preferred areseeds, seedlings, tubers or fruits as harvestable or propagationmaterial.

The invention furthermore relates to the use of the transgenic organismsaccording to the invention and of the cells, cell cultures, parts—suchas, for example, roots, leaves and the like as mentioned above in thecase of transgenic plant organisms—derived from them, and to transgenicpropagation material such as seeds or fruits and the like as mentionedabove, for the production of foodstuffs or feeding stuffs,pharmaceuticals or fine chemicals.

Accordingly in another embodiment, the present invention relates to theuse of the nucleic acid molecule, the organism, e.g. the microorganism,the plant, plant cell or plant tissue, the vector, or the polypeptide ofthe present invention for making the fine chemical. Fine chemicals inthe sense of the inventions are for example fatty acids, carotenoids,isoprenoids, vitamins, lipids, wax esters, (poly)saccharides and/orpolyhydroxyalkanoates, and/or its metabolism products, in particular,steroid hormones, cholesterol, prostaglandin, triacylglycerols, bileacids and/or ketone bodies, which are produced in cells, tissues and/orplants. There are a number of mechanisms by which the yield, production,and/or efficiency of production of fatty acids, carotenoids,isoprenoids, vitamins, wax esters, lipids, (poly)saccharides and/orpolyhydroxyalkanoates, and/or its metabolism products, in particular,steroid hormones, cholesterol, triacylglycerols, prostaglandin, bileacids and/or ketone bodies or further of above defined fine chemicalsincorporating such an altered protein can be affected. In the case ofplants, by e.g. increasing the expression of acetyl-CoA which is thebasis for many products, e.g., fatty acids, carotenoids, isoprenoids,vitamines, lipids, (poly)saccharides, wax esters, and/orpolyhydroxyalkanoates, and/or its metabolism products, in particular,prostaglandin, steroid hormones, cholesterol, triacylglycerols, bileacids and/or ketone bodies in a cell, it may be possible to increase theamount of the produced said compounds thus permitting greater ease ofharvesting and purification or in case of plants more efficientpartitioning. Further, one or more of said metabolism products,increased amounts of the cofactors, precursor molecules, andintermediate compounds for the appropriate biosynthetic pathways may berequired. Therefore, by increasing the number and/or activity oftransporter proteins involved in the import of nutrients, such as carbonsources (i.e., sugars), nitrogen sources (i.e., amino acids, ammoniumsalts), phosphate, and sulfur, it may be possible to improve theproduction of acetyl CoA and its metabolism products as mentioned above,due to the removal of any nutrient supply limitations on thebiosynthetic process. In particular, it may be possible to increase theyield, production, and/or efficiency of production of said compounds,e.g. fatty acids, carotenoids, isoprenoids, vitamins, was esters,lipids, (poly)saccharides, and/or polyhydroxyalkanoates, and/or itsmetabolism products, in particular, steroid hormones, cholesterol,prostaglandin, triacylglycerols, bile acids and/or ketone bodiesmolecules etc. in plants.

Furthermore preferred is a method for the recombinant production ofpharmaceuticals or fine chemicals in host organisms, wherein a hostorganism is transformed with one of the above-described nucleic acidconstructs comprising one or more structural genes which encode thedesired fine chemical or catalyze the biosynthesis of the desired finechemical, the transformed host organism is cultured, and the desiredfine chemical is isolated from the culture medium. This method can beapplied widely to fine chemicals such as enzymes, vitamins, amino acids,sugars, fatty acids, and natural and synthetic flavorings, aromasubstances and colorants or compositions comprising these. Especiallypreferred is the additional production of further amino acids,tocopherols and tocotrienols and carotenoids or compositions comprisingsaid compounds. The transformed host organisms are cultured and theproducts are recovered from the host organisms or the culture medium bymethods known to the skilled worker or the organism itself servers asfood or feed supplement. The production of pharmaceuticals such as, forexample, antibodies or vaccines, is described by Hood EE, Jilka J M.Curr Opin Biotechnol. 1999 August; 10(4):382-6; Ma J K, Vine N D. CurrTop Microbiol Immunol. 1999; 236:275-92.

Another embodiment of the invention is a double-stranded RNA molecule(dsRNA), whereby the sense strand of said double-stranded RNA nucleicacid molecule has a homology of at least 30%, 35%, 40%, 45%, 50%, 55% or60%, preferably 65%, 70%, 75% or 80%, more preferably 85%, 90%, 95%,96%, 97%, 98% or 99% to the nucleic acid molecule of the invention orencoding a protein conferring the expression of a protein having thebiological activity of the protein of the invention or comprising afragment of at least 10 base pairs (=bases, nt, nucleotides), preferablyat least 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40,45 or 50, especially preferably at least 40, 50, 60, 70, 80 or 90 basepairs, very especially preferably at least 100, 200, 300 or 400 basepairs, most preferably at least 500, 600, 700, 800, 900 or more basepairs or at least 1000 or 2000 base pairs of a nucleic acid moleculewith a homology of at least 50%, 60%, 70%, 80% or 90%, preferably 100%to a nucleic acid molecule conferring the expression of a protein havingthe biological activity of the protein of the invention or to thenucleic acid molecule of the invention.

In another preferred embodiment of the invention the encoded sequence orits part-segment amounts to 17, 18, 19, 20, 21, 22, 23, 24, 25, 26 or 27bases, preferably to 20, 21, 22, 23, 24 or 25 bases, whereby thehomology of the sequence is similar to the aforementioned homologies.

In a preferred embodiment of the invention the sense and antisensestrand of the double-stranded RNA are covalently bound or are bound byweak chemical bonds such as hydrogen bonds to each other and theantisense strand is essentially the complement of the sense-RNA strand.

Yet another embodiment of the invention is an antisense nucleic acidmolecule, whereby the antisense nucleic acid molecule has a homology ofat least 30% to a nucleic acid molecule antisense to a nucleic acidmolecule encoding the protein encoded by the nucleic acid molecule ofthe invention or the nucleic acid molecule of the invention or encodingthe protein of the invention and conferring the expression of a proteinhaving the biological activity of a protein having the biologicalactivity of the protein of the invention or an antisense nucleic acidmolecule comprising a fragment of at least 15, 16, 17, 18, 19, 20, 21,22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45 or 50, especiallypreferably at least 60, 70, 80 or 90 base pairs, very especiallypreferably at least 100, 200, 300 or 400 base pairs, most preferably atleast 500, 600, 700, 800, 900 or more base pairs or at least the entiresequence of a nucleic acid molecule with a homology of at least 50% 60%,70%, 80% or 90%, preferably 100% to an antisense nucleic acid moleculeto a nucleic acid molecule conferring the expression of a protein havingthe biological activity of the protein of the invention or to thenucleic acid molecule of the invention itself or to a nucleic acidencoding the protein of the invention.

A further embodiment of the invention is a ribozyme, which specificallycleaves a nucleic acid molecule conferring expression of a proteinhaving the biological activity of the protein of the invention or anucleic acid molecule encoding the protein encoded by the nucleic acidmolecule of the invention or the nucleic acid molecule of the inventionitself.

A further embodiment of the invention is an antibody, which specificallybinds to and therefore inhibits proteins encoded nucleic acid moleculeconferring expression of a protein having the biological activity of theprotein of the invention or a nucleic acid molecule encoding the proteinencoded by the nucleic acid molecule of the invention or the nucleicacid molecule of the invention itself.

Yet another embodiments of the invention are a viral nucleic acidmolecule conferring the decline of a RNA molecule conferring theexpression of a protein having the biological activity of the protein ofthe invention or of a nucleic acid molecule encoding the protein encodedby the nucleic acid molecule of the invention or of the nucleic acidmolecule of the invention or a dominant-negative mutant of the proteinof the invention or a nucleic acid molecule encoding such adominant-negative mutant.

In one embodiment, the present invention relates to a method for theidentification of a gene product conferring an increase in the finechemical production in a cell of an organism for example amicroorganism, a non-human animal or plant, comprising the followingsteps:

-   (a) contacting e.g. hybridising, the nucleic acid molecules of a    sample, e.g. cells, tissues, plants or microorganisms or a nucleic    acid library, which can contain a candidate gene encoding a gene    product conferring an increase in the fine chemical after reduction    or deletion of its expression, with the nucleic acid molecule of the    present invention;-   (b) identifying the nucleic acid molecules, which hybridize under    relaxed stringent conditions with the nucleic acid molecule of the    present invention in particular to the nucleic acid molecule    sequence shown in SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID    NO: 7, SEQ ID NO: 9, SEQ ID NO: 11, SEQ ID NO: 13, SEQ ID NO: 15,    SEQ ID NO: 19, SEQ ID NO: 21, SEQ ID NO: 23, SEQ ID NO: 25, SEQ ID    NO: 27, SEQ ID NO: 29, SEQ ID NO: 31; SEQ ID NO: 64, SEQ ID NO: 66,    SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID    NO: 76, SEQ ID NO: 78, SEQ ID NO: 80, SEQ ID NO: 82, SEQ ID NO: 84,    SEQ ID NO: 86, SEQ ID NO: 88, SEQ ID NO: 90, SEQ ID NO: 92, SEQ ID    NO: 94, SEQ ID NO: 96, SEQ ID NO: 98, SEQ ID NO: 100, SEQ ID NO:    102, SEQ ID NO: 104, SEQ ID NO: 106, SEQ ID NO: 108, SEQ ID NO: 110,    SEQ ID NO: 112, SEQ ID NO: 114, SEQ ID NO: 116, SEQ ID NO: 118, SEQ    ID NO: 120, SEQ ID NO: 122, SEQ ID NO: 124, SEQ ID NO: 126, SEQ ID    NO: 128, SEQ ID NO: 130, SEQ ID NO: 132, SEQ ID NO: 134, SEQ ID NO:    136, SEQ ID NO: 138, SEQ ID NO: 140, SEQ ID NO: , SEQ ID NO: 142,    SEQ ID NO: 151, SEQ ID NO: 153, SEQ ID NO: 155, SEQ ID NO: 157, SEQ    ID NO: 159, SEQ ID NO: 161, SEQ ID NO: 163, SEQ ID NO: 165, SEQ ID    NO: 167, SEQ ID NO: 169, SEQ ID NO: 171, SEQ ID NO: 173, SEQ ID NO:    175, SEQ ID NO: 177, SEQ ID NO: 179, SEQ ID NO: 181, SEQ ID NO: 183,    SEQ ID NO: 185, SEQ ID NO: 187, SEQ ID NO: 189, SEQ ID NO: 191, SEQ    ID NO: 193, SEQ ID NO: 195, SEQ ID NO: 197, SEQ ID NO: 199, SEQ ID    NO: 201, SEQ ID NO: 203, SEQ ID NO: 205, SEQ ID NO: 207, SEQ ID NO:    209, SEQ ID NO: 211, SEQ ID NO: 213, SEQ ID NO: 215, SEQ ID NO: 217,    SEQ ID NO: 219, SEQ ID NO: 228, SEQ ID NO: 230, SEQ ID NO: 232, SEQ    ID NO: 234, SEQ ID NO: 236, SEQ ID NO: 238, SEQ ID NO: 240, SEQ ID    NO: 242, SEQ ID NO: 249, SEQ ID NO: 251, SEQ ID NO: 253, SEQ ID NO:    255, SEQ ID NO: 257, SEQ ID NO: 259, SEQ ID NO: 261, SEQ ID NO: 263,    SEQ ID NO: 265, SEQ ID NO: 267, SEQ ID NO: 269, SEQ ID NO: 271, SEQ    ID NO: 282, SEQ ID NO: 284, SEQ ID NO: 286, SEQ ID NO: 288, SEQ ID    NO: 290, SEQ ID NO: 292, SEQ ID NO: 294, SEQ ID NO: 296, SEQ ID NO:    298, SEQ ID NO: 300, SEQ ID NO: 302, SEQ ID NO: 304, SEQ ID NO: 306,    SEQ ID NO: 308, SEQ ID NO: 310, SEQ ID NO: 312, SEQ ID NO: 314, SEQ    ID NO: 316, SEQ ID NO: 318, SEQ ID NO: 320, SEQ ID NO: 322, SEQ ID    NO: 324, SEQ ID NO: 326, SEQ ID NO: 328, SEQ ID NO: 330, SEQ ID NO:    332, SEQ ID NO: 334, SEQ ID NO: 336, SEQ ID NO: 338, SEQ ID NO: 340,    SEQ ID NO: 342, SEQ ID NO: 344, SEQ ID NO: 346, SEQ ID NO: 348, SEQ    ID NO: 350, SEQ ID NO: 352, SEQ ID NO: 354, SEQ ID NO: 356, SEQ ID    NO: 358, SEQ ID NO: 360, SEQ ID NO: 362, SEQ ID NO: 364, SEQ ID NO:    366, SEQ ID NO: 368, SEQ ID NO: 370, SEQ ID NO: 372, SEQ ID NO: 374,    SEQ ID NO: 376, SEQ ID NO: 378, SEQ ID NO: 380, SEQ ID NO: 382, SEQ    ID NO: 384, SEQ ID NO: 386, SEQ ID NO: 388, SEQ ID NO: 390, SEQ ID    NO: 392, SEQ ID NO: 394, SEQ ID NO: 396, SEQ ID NO: 398, SEQ ID NO:    400, SEQ ID NO: 402, SEQ ID NO: 404, SEQ ID NO: 406, SEQ ID NO: 408,    SEQ ID NO: 410, SEQ ID NO: 412, SEQ ID NO: 414, SEQ ID NO: 416, SEQ    ID NO: 418, SEQ ID NO: 420, SEQ ID NO: 422, SEQ ID NO: 424, SEQ ID    NO: 426, SEQ ID NO: 428, SEQ ID NO: 430, SEQ ID NO: 432, SEQ ID NO:    434 or SEQ ID NO: 440 and, optionally, isolating the full length    cDNA clone or complete genomic clone;-   (c) introducing the candidate nucleic acid molecules in host cells,    preferably in a plant cell or a microorganism, appropriate for    producing the fine chemical-   (d) reducing or deletion the expressing of the identified nucleic    acid molecules in the host cells;-   (e) assaying the fine chemical level in the host cells; and-   (f) identifying the nucleic acid molecule and its gene product which    reduction or deletion of expression confers an increase in the fine    chemical level in the host cell after expression compared to the    wild type.

Relaxed hybridisation conditions are: After standard hybridisationprocedures washing steps can be performed at low to medium stringencyconditions usually with washing conditions of 40°-55° C. and saltconditions between 2×SSC and 0.2×SSC with 0.1% SDS in comparison tostringent washing conditions as e.g. 60° to 68° C. with 0.1% SDS.Further examples can be found in the references listed above for thestringed hybridization conditions. Usually washing steps are repeatedwith increasing stringency and length until a useful signal to noiseratio is detected and depend on many factors as the target, e.g. itspurity, GC-content, size etc, the probe, e.g. its length, is it a RNA ora DNA probe, salt conditions, washing or hybridisation temperature,washing or hybridisation time etc.

In another embodiment, the present invention relates to a method for theidentification of a gene product the reduction of which conferring anincreased production of the fine chemical in a cell, comprising thefollowing steps:

-   (a) identifying nucleic acid molecules of an organism; which can    contain a candidate gene encoding a gene product conferring an    increase in the fine chemical after reduction or deletion of its    expression, which are at least 20%, preferably 25%, more preferably    30%, even more preferred are 35%. 40% or 50%, even more preferred    are 60%, 70% or 80%, most preferred are 90% or 95% or more homolog    to the nucleic acid molecule of the present invention, for example    via homology search in a data bank;-   (b) reducing or deleting the expression of the identified nucleic    acid molecules in the host cells;-   (c) assaying the fine chemical level in the host cells; and-   (d) identifying the nucleic acid molecule and its gene product which    reduction or deletion of expression confers an increase in the fine    chemical level in the host cell compared to the wild type

The nucleic acid molecules identified can then be used for theproduction of the fine chemical in the same way as the nucleic acidmolecule of the present invention. Accordingly, in one embodiment, thepresent invention relates to a process for the production of the finechemical, comprising (a) identifying a nucleic acid molecule accordingto aforementioned steps (a) to (d) and recovering the free or boundcompound of the invention from a organism having an decreased cellularactivity of a polypeptide encoded by the isolated nucleic acid moleculecompared to a wild type.

Furthermore, in one embodiment, the present invention relates to amethod for the identification of a compound stimulating the finechemical production to said plant comprising:

-   a) contacting cells which express the polypeptide of the present    invention or its mRNA with a candidate compound under cell    cultivation conditions;-   b) assaying a reduction, decrease or deletion in expression of said    polypeptide or said mRNA;-   c) comparing the expression level to a standard response made in the    absence of said candidate compound; whereby, a reduced, decreased or    deleted expression over the standard indicates that the compound is    stimulating the fine chemical production.

Furthermore, in one embodiment, the present invention relates to amethod for the screening for antagonists of the activity of thepolypeptide of the present invention, e.g. a polypeptide conferring anincrease of the fine chemical in an organism or a part thereof afterdecreasing the cellular activity, e.g. of the activity of a polypeptidehaving the biological activity represented by the protein of theinvention comprising:

-   (a) contacting cells, tissues, plants or microorganisms which    express the polypeptide according to the invention with a candidate    compound or a sample comprising a plurality of compounds under    conditions which permit the expression the polypeptide of the    present invention;-   (b) assaying the fine chemical level or the polypeptide expression    level in the cell, tissue, plant or microorganism or the media the    cell, tissue, plant or microorganisms is cultured or maintained in;    and-   (c) identifying an antagonist by comparing the measured fine    chemical level or polypeptide expression level with a standard fine    chemical or polypeptide expression level measured in the absence of    said candidate compound or a sample comprising said plurality of    compounds, whereby an increased level of the fine chemical over the    standard indicates that the compound or the sample comprising said    plurality of compounds is an antagonist.

Yet another embodiment of the invention relates to a process for theidentification of a compound conferring increased fine chemicalproduction in a plant; non-human animal or microorganism, comprising thefollowing step:

-   a. culturing or maintaining a plant or animal cell or their tissues    or microorganism expressing the polypeptide of the invention or a    polynucleotide encoding said polypeptide and a readout system    capable of interacting with the polypeptide under suitable    conditions which permit the interaction of the polypeptide with this    readout system in the presence of a chemical compound or a sample    comprising a plurality of chemical compounds and capable of    providing a detectable signal in response to the binding of a    chemical compound to said polypeptide under conditions which permit    the depression of said readout system and of the protein of the    invention; and-   b. identifying if the chemical compound is an effective antagonist    by detecting the presence or absence or decrease or increase of a    signal produced by said readout system.

Said compound may be chemically synthesized or microbiologicallyproduced and/or comprised in, for example, samples, e.g., cell extractsfrom, e.g., plants, animals or microorganisms, e.g. pathogens.Furthermore, said compound(s) may be known in the art but hitherto notknown to be capable of suppressing the polypeptide of the presentinvention. The reaction mixture may be a cell free extract or maycomprise a cell or tissue culture. Suitable set ups for the method ofthe invention are known to the person skilled in the art and are, forexample, generally described in Alberts et al., Molecular Biology of theCell, third edition (1994), in particular Chapter 17. The compounds maybe, e.g., added to the reaction mixture, culture medium, injected intothe cell or sprayed onto the plant.

If a sample containing a compound is identified in the method of theinvention, then it is either possible to isolate the compound from theoriginal sample identified as containing the compound capable ofactivating or increasing the content of the fine chemical in an organismor part thereof, or one can further subdivide the original sample, forexample, if it consists of a plurality of different compounds, so as toreduce the number of different substances per sample and repeat themethod with the subdivisions of the original sample. Depending on thecomplexity of the samples, the steps described above can be performedseveral times, preferably until the sample identified according to themethod of the invention only comprises a limited number of or only onesubstance(s). Preferably said sample comprises substances of similarchemical and/or physical properties, and most preferably said substancesare identical. Preferably, the compound identified according to thedescribed method above or its derivative is further formulated in a formsuitable for the application in plant breeding or plant cell and tissueculture.

The compounds which can be tested and identified according to a methodof the invention may be expression libraries, e.g., cDNA expressionlibraries, peptides, proteins, nucleic acids, antibodies, small organiccompounds, hormones, peptidomimetics, PNAs or the like (Milner, NatureMedicine 1 (1995), 879-880; Hupp, Cell 83 (1995), 237-245; Gibbs, Cell79 (1994), 193-198 and references cited supra). Said compounds can alsobe functional derivatives or analogues of known inhibitors oractivators. Methods for the preparation of chemical derivatives andanalogues are well known to those skilled in the art and are describedin, for example, Beilstein, Handbook of Organic Chemistry, Springeredition New York Inc., 175 Fifth Avenue, New York, N.Y. 10010 U.S.A. andOrganic Synthesis, Wiley, New York, USA. Furthermore, said derivativesand analogues can be tested for their effects according to methods knownin the art. Furthermore, peptidomimetics and/or computer aided design ofappropriate derivatives and analogues can be used, for example,according to the methods described above. The cell or tissue that may beemployed in the method of the invention preferably is a host cell, plantcell or plant tissue of the invention described in the embodimentshereinbefore.

Thus, in a further embodiment the invention relates to a compoundobtained or identified according to the method for identifying anantagonist of the invention said compound being an antagonist of thepolypeptide of the present invention.

Accordingly, in one embodiment, the present invention further relates toa compound identified by the method for identifying a compound of thepresent invention.

Said compound is, for example, a homolog of the polypeptide of thepresent invention. Homologues of the polypeptide of the presentinvention can be generated by mutagenesis, e.g., discrete point mutationor truncation of the polypeptide of the present invention. As usedherein, the term “homologue” refers to a variant form of the protein,which acts as an antagonist of the activity of the polypeptide of thepresent invention. An antagonist of said protein has lost the biologicalactivities of the polypeptide of the present invention. In particular,said antagonist confers a decrease of the expression level of thepolypeptide of the present invention and thereby the expression of saidantagonist in an organisms or part thereof confers the increase of freeand/or bound compound of the invention in the organism or part thereof.

In one embodiment, the invention relates to an antibody specificallyrecognizing the compound or antagonist of the present invention.

The invention also relates to a diagnostic composition comprising atleast one of the aforementioned nucleic acid molecules, vectors,proteins, antibodies or compounds of the invention and optionallysuitable means for detection.

The diagnostic composition of the present invention is suitable for theisolation of mRNA from a cell and contacting the mRNA so obtained with aprobe comprising a nucleic acid probe as described above underhybridizing conditions, detecting the presence of mRNA hybridized to theprobe, and thereby detecting the expression of the protein in the cell.Further methods of detecting the presence of a protein according to thepresent invention comprise immunotechniques well known in the art, forexample enzyme linked immunoabsorbent assay. Furthermore, it is possibleto use the nucleic acid molecules according to the invention asmolecular markers or primers in plant breeding. Suitable means fordetection are well known to a person skilled in the art, e.g. buffersand solutions for hydridization assays, e.g. the aforementionedsolutions and buffers, further and means for Southern-, Western-,Northern etc. -blots, as e.g. described in Sambrook et al. are known.

In another embodiment, the present invention relates to a kit comprisingthe nucleic acid molecule, the vector, the host cell, the polypeptide,the anti-sense nucleic acid, the antibody, plant cell, the plant orplant tissue, the harvestable part, the propagation material and/or thecompound and/or antagonist identified according to the method of theinvention.

The compounds of the kit of the present invention may be packaged incontainers such as vials, optionally with/in buffers and/or solution. Ifappropriate, one or more of said components might be packaged in one andthe same container. Additionally or alternatively, one or more of saidcomponents might be adsorbed to a solid support as, e.g. anitrocellulose filter, a glass plate, a chip, or a nylon membrane or tothe well of a micro titerplate. The kit can be used for any of theherein described methods and embodiments, e.g. for the production of thehost cells, transgenic plants, pharmaceutical compositions, detection ofhomologous sequences, identification of antagonists or agonists, as foodor feed or as a supplement thereof or as supplement for the treating ofplants, etc.

Further, the kit can comprise instructions for the use of the kit forany of said embodiments, in particular for the use for producingorganisms or part thereof having an increased free or bound compound ofthe invention content.

In one embodiment said kit comprises further a nucleic acid moleculeencoding one or more of the aforementioned protein, and/or an antibody,a vector, a host cell, an antisense nucleic acid, a plant cell or planttissue or a plant.

In a further embodiment, the present invention relates to a method forthe production of a agricultural composition providing the nucleic acidmolecule, the vector or the polypeptide of the invention or comprisingthe steps of the method according to the invention for theidentification of said compound or antagonist; and formulating thenucleic acid molecule, the vector or the polypeptide of the invention orthe antagonist, or compound identified according to the methods orprocesses of the present invention or with use of the subject matters ofthe present invention in a form applicable as plant agriculturalcomposition.

In another embodiment, the present invention relates to a method for theproduction of the fine chemical supporting plant culture compositioncomprising the steps of the method for of the present invention; andformulating the compound identified in a form acceptable as agriculturalcomposition.

Under “acceptable as agricultural composition” is understood, that sucha composition is in agreement with the laws regulating the content offungicides, plant nutrients, herbicides, etc. Preferably such acomposition is without any harm for the protected plants and the animals(humans included) fed therewith.

The present invention also pertains to several embodiments relating tofurther uses and methods. The nucleic acid molecule, polypeptide,protein homologues, fusion proteins, primers, vectors, host cells,described herein can be used in one or more of the following methods:identification of plants useful for the fine chemical production asmentioned and related organisms; mapping of genomes; identification andlocalization of sequences of interest; evolutionary studies;determination of regions required for function; modulation of anactivity.

The nucleic acid molecule of the invention, the vector of the inventionor the nucleic acid construct of the invention may also be useful forthe production of organisms resistant to inhibitors of the amino acidproduction biosynthesis pathways. In particular, the reduction, decreaseor deletion of the polypeptide of the present invention may protectplants against herbicides, which block the amino acid, in particular themethionine, synthesis in said plant.

Accordingly, the nucleic acid molecules of the present invention have avariety of uses. First, they may be used to identify an organism or aclose relative thereof. Also, they may be used to identify the presencethereof or a relative thereof in a mixed population of microorganisms orplants. By probing the extracted genomic DNA of a culture of a unique ormixed population of plants under stringent conditions with a probespanning a region of the gene of the present invention which is uniqueto this, one can ascertain whether the present invention has been usedor whether it or a close relative is present.

Further, the nucleic acid molecule of the invention may be sufficientlyhomologous to the sequences of related species such that these nucleicacid molecules may serve as markers for the construction of a genomicmap in related organism or for association mapping. Furthermore naturalvariation in the genomic regions corresponding to nucleic acids of theinvention or homologous thereof may lead to variation in the activity ofthe proteins of the invention and their homolgous and in consequence innatural variation in the production of the fine chemical. In consequencenatural variation eventually also exists in form of less active allelicvariants leading already to a relative increase in the production of thefine chemical. Different variants of the nucleic acids of the invention,which corresponds to different fine chemical production capabilities canbe identified and used for marker assisted breeding for increase in thefine chemical.

Accordingly, the present invention relates to a method for breedingplants for the production of the fine chemical, comprising

-   (a) selecting a first plant variety capable to produce the fine    chemical by reducing decreasing or deleting the expressing the    polypeptide according to invention;-   (b) associating the ability to produce a certain level of the fine    chemical with the expression level or the genomic structure of the    genes of the invention;-   (c) crossing the first plant variety with a second plant variety,    which significantly differs in its ability to produce the fine    chemical; and-   (d) identifying, which of the offspring varieties has got the    capacity to overproduce the fine chemical by means of analyzing the    expression or genomic structure of the genes of the invention.

The nucleic acid molecules of the invention are also useful forevolutionary and protein structural studies. By comparing the sequencesof the invention to those encoding similar enzymes from other organisms,the evolutionary relatedness of the organisms can be assessed.Similarly, such a comparison permits an assessment of which regions ofthe sequence are conserved and which are not, which may aid indetermining those regions of the protein which are essential for thefunctioning of the enzyme. This type of determination is of value forprotein engineering studies and may give an indication of what theprotein can tolerate in terms of mutagenesis without losing function.

Accordingly, the nucleic acid molecule of the invention can be used forthe identification of other nucleic acids conferring an increase of thefine chemical after reduction, decrease or deletion of their expression.

Further, the nucleic acid molecule of the invention or a fragment of agene conferring the expression of the polypeptide of the invention,preferably comprising the nucleic acid molecule of the invention, can beused for marker assisted breeding or association mapping of the finechemical derived traits.

Accordingly, the nucleic acid of the invention, the polypeptide of theinvention, the nucleic acid construct of the invention, the organisms,the host cell, the microorganisms, the plant, plant tissue, plant cell,or the part thereof of the invention, the vector of the invention, theantagonist identified with the method of the invention, the nucleic acidmolecule identified with the method of the present invention, can beused for the production of the fine chemical or the fine chemical incombination with one or more amino acids, in particular threonine,alanine, homoserine, glutamine, glutamic acid, valine, asparagine,phenylalanine, leucine, isoleucine, proline and/or tryptophane.

These and other embodiments are disclosed and encompassed by thedescription and examples of the present invention. Further literatureconcerning any one of the methods, uses and compounds to be employed inaccordance with the present invention may be retrieved from publiclibraries, using for example electronic devices. For example the publicdatabase “Medline” may be utilized which is available on the Internet,for example under the web site for ncbi.nlm.nih.gov/PubMed/medline.html.Further databases and addresses, such as the web sites forncbi.nlm.nih.gov/, infobiogen.fr/, fmi.ch/biology/research-tools.html,tigr.org/, are known to the person skilled in the art and can also beobtained using, e.g., the web site for lycos.com. An overview of patentinformation in biotechnology and a survey of relevant sources of patentinformation useful for retrospective searching and for current awarenessis given in Berks, TIBTECH 12 (1994), 352-364.

The present invention is illustrated by the examples, which follow. Thepresent examples illustrate the basic invention without being intendedas limiting the subject of the invention. The content of all of thereferences, patent applications, patents and published patentapplications cited in the present patent application is herewithincorporated by reference.

EXAMPLES Example 1 Cloning SEQ ID NO: 1 in Escherichia coli

SEQ ID NO: 1 can be cloned into the plasmids pBR322 (Sutcliffe, J. G.(1979) Proc. Natl Acad. Sci. USA, 75: 3737-3741); pACYC177 (Change &Cohen (1978) J. Bacteriol. 134: 1141-1156); plasmids of the pBS series(pBSSK+, pBSSK− and others; Stratagene, LaJolla, USA) or cosmids such asSuperCos1 (Stratagene, LaJolla, USA) or Lorist6 (Gibson, T. J.Rosenthal, A., and Waterson, R. H. (1987) Gene 53: 283-286) forexpression in E. coli using known, well-established procedures (see, forexample, Sambrook, J. et al. (1989) “Molecular Cloning: A LaboratoryManual”. Cold Spring Harbor Laboratory Press or Ausubel, F. M. et al.(1994) “Current Protocols in Molecular Biology”, John Wiley & Sons).

Unless otherwise specified, standard methods as described in Sambrook etal., Molecular Cloning: A laboratory manual, Cold Spring Harbor 1989,Cold Spring Harbor Laboratory Press are used throughout the examples.

Example 2 DNA Sequencing and Computerized Functional Analysis

The DNA was sequenced by standard procedures, in particular the chaindetermination method, using ABI377 sequencers (see, for example,Fleischman, R. D. et al. (1995) “Whole-genome Random Sequencing andAssembly of Haemophilus Influenza Rd., Science 269; 496-512)”.

Example 3 In-Vivo Mutagenesis

An in vivo mutagenesis of Corynebacterium glutamicum for the productionof amino acids can be carried out by passing a plasmid DNA (or anothervector DNA) through E. coli such as E. coli XL-1 red and othermicroorganisms (for example Bacillus spp. or yeasts such asSaccharomyces cerevisiae), which are not capable of maintaining theintegrity of its genetic information. Usual mutator strains havemutations in the genes for the DNA repair system [for example mutHLS,mutD, mutT and the like; for comparison, see Rupp, W. D. (1996) DNArepair mechanisms in Escherichia coli and Salmonella, pp. 2277-2294,ASM: Washington]. These strains are known to the skilled worker. The useof these strains is illustrated for example in Greener, A. and Callahan,M. (1994) Strategies 7; 32-34.

Example 4 DNA Transfer Between Escherichia coli and Corynebacteriumglutamicum

Several Corynebacterium and Brevibacterium species comprise endogenousplasmids (such as, for example, pHM1519 or pBL1), which replicateautonomously (for a review, see, for example, Martin, J. F. et al.(1987) Biotechnology 5: 137-146). Shuttle vectors for Escherichia coliand Corynebacterium glutamicum can be constructed easily using standardvectors for E. coli (Sambrook, J. et al., (1989), “Molecular Cloning: ALaboratory Manual”, Cold Spring Harbor Laboratory Press or Ausubel, F.M. et al. (1994) “Current Protocols in Molecular Biology”, John Wiley &Sons), which have a replication origin for, and suitable marker from,Corynebacterium glutamicum added. Such replication origins arepreferably taken from endogenous plasmids, which have been isolated fromCorynebacterium and Brevibacterium species. Genes, which are used inparticular as transformation markers for these species are genes forkanamycin resistance (such as those which originate from the Tn5 orTn-903 transposon) or for chloramphenicol resistance (Winnacker, E. L.(1987) “From Genes to Clones—Introduction to Gene Technology, VCH,Weinheim). There are many examples in the literature of the preparationof a large multiplicity of shuttle vectors which are replicated in E.coli and C. glutamicum and which can be used for various purposesincluding the overexpression of genes (see, for example, Yoshihama, M.et al. (1985) J. Bacteriol. 162: 591-597, Martin, J. F. et al., (1987)Biotechnology, 5: 137-146 and Eikmanns, B. J. et al. (1992) Gene 102:93-98) or the downregulation of genes. Suitable vectors, which replicatein coryneform bacteria are for example, pZ1 (Menkel et al., Appl.Environ. Microbiol. 64, 1989: 549-554) pEkEx1 (Eikmanns et al., Gene102, 1991: 93-98) or pHS2-1 (Sonnen et al, Gene 107, 1991: 69-74). Thesevectors are based on the cryptic plasmids pHM1519, pBL1 or pGA1. Otherplasmid vectors such as, for example, those based on pCG4 (U.S. Pat. No.4,489,160), pNG2 (Serwold-Davis et al., FEMS Microbiol. Lett., 66, 1990:119-124) or pAG1 (U.S. Pat. No. 5,158,891) can be used in the samemanner.

Using standard methods, it is possible to clone a gene of interest intoone of the above-described shuttle vectors and to introduce such hybridvectors into Corynebacterium glutamicum strains. The transformation ofC. glutamicum can be achieved by protoplast transformation (Kastsumata,R. et al., (1984) J. Bacteriol. 159, 306-311), electroporation (Liebl,E. et al., (1989) FEMS Microbiol. Letters, 53: 399-303) and in thosecases where specific vectors are used also by conjugation (such as, forexample, described in Schäfer, A., et al. (1990) J. Bacteriol. 172:1663-1666). Likewise, it is possible to transfer the shuttle vectors forC. glutamicum to E. coli by preparing plasmid DNA from C. glutamicum(using standard methods known in the art) and transforming it into E.coli. This transformation step can be carried out using standardmethods, but preferably using a Mcr-deficient E. coli strain, such asNM522 (Gough & Murray (1983) J. Mol. Biol. 166: 1-19).

If the transformed sequence(s) is/are to be integrated advantageouslyinto the genome of the coryneform bacteria, standard techniques known tothe skilled worker also exist for this purpose. Examples, which are usedfor this purpose are plasmid vectors as they have been described byRemscheid et al. (Appl. Environ. Microbiol., 60, 1994: 126-132) for theduplication and amplification of the hom-thrB operon. In this method,the complete gene is cloned into a plasmid vector, which is capable ofreplication in a host such as E. coli, but not in C. glutamicum.Suitable vectors are, for example, pSUP301 (Simon et al., Bio/Technology1, 1983: 784-791), pKIBmob or pK19mob (Schäfer et al., Gene 145, 1994:69-73), pGEM-T (Promega Corp., Madison, Wis., USA), pCR2.1-TOPO(Schuman, J. Biol. Chem., 269, 1994: 32678-32684, U.S. Pat. No.5,487,993), pCR®Blunt (Invitrogen, Groningen, the Netherlands) or pEM1(Schrumpf et al., J. Bacteriol., 173, 1991: 4510-4516).

Another method to introduce mutations in a target gene is disclosed bySchafer et al. [Gene. 1994 Jul. 22; 145(1):69-73]. Schafer et al.teaches small mobilizable multi-purpose cloning vectors derived from theEscherichia coli plasmids pK18 and pK19 for the selection of defineddeletions in the chromosome of Corynebacterium glutamicum.

Example 5 Determining the Expression of the Mutant/Transgenic Protein

The observations of the activity of a mutated, or transgenic, protein ina transformed host cell are based on the fact that the protein isexpressed in a modified manner and in therefore for example in lowerquantity as the wild-type protein. A suitable method for determining thetranscription quantity of the mutant, or transgenic, gene (a sign forthe amount of mRNA which is available for the translation of the geneproduct) is to carry out a Northern blot (see, for example, Ausubel etal., (1988) Current Protocols in Molecular Biology, Wiley: New York),where a primer which is designed in such a way that it binds to the geneof interest is provided with a detectable marker (usually a radioactiveor chemiluminescent marker) so that, when the total RNA of a culture ofthe organism is extracted, separated on a gel, applied to a stablematrix and incubated with this probe, the binding and quantity of thebinding of the probe indicates the presence and also the amount of mRNAfor this gene. Another method is a quantitative PCR. This informationdetects the extent to which the gene has been transcribed. Total cellRNA can be isolated from Corynebacterium glutamicum by a variety ofmethods, which are known in the art, as described in Bormann, E. R. etal., (1992) Mol. Microbiol. 6: 317-326.

Standard techniques, such as Western blot, may be employed to determinethe presence or relative amount of protein translated from this mRNA(see, for example, Ausubel et al. (1988) “Current Protocols in MolecularBiology”, Wiley, New York). In this method, total cell proteins areextracted, separated by gel electrophoresis, transferred to a matrixsuch as nitrocellulose and incubated with a probe, such as an antibody,which binds specifically to the desired protein. This probe is usuallyprovided directly or indirectly with a chemiluminescent or colorimetricmarker, which can be detected readily. The presence and the observedamount of marker indicate the presence and the amount of the soughtmutant protein in the cell. However, other methods are also known.

Example 6 Growth of Genetically Modified Corynebacterium glutamicum:Media and Culture Conditions

Genetically modified Corynebacteria are grown in synthetic or naturalgrowth media. A number of different growth media for Corynebacteria areknown and widely available (Lieb et al. (1989) Appl. Microbiol.Biotechnol. 32: 205-210; von der Osten et al. (1998) BiotechnologyLetters 11: 11-16; Patent DE 4 120 867; Liebl (1992) “The GenusCorynebacterium”, in: The Procaryotes, Vol. II, Balows, A., et al.,Ed-Springer-Verlag).

Said media, which can be used according to the invention usually consistof one or more carbon sources, nitrogen sources, inorganic salts,vitamins and trace elements. Preferred carbon sources are sugars such asmono-, di- or polysaccharides. Examples of very good carbon sources areglucose, fructose, mannose, galactose, ribose, sorbose, ribulose,lactose, maltose, sucrose, raffinose, starch or cellulose. Sugars mayalso be added to the media via complex compounds such as molasses orother by-products of sugar refining. It may also be advantageous to addmixtures of various carbon sources. Other possible carbon sources arealcohols and/or organic acids such as methanol, ethanol, acetic acid orlactic acid. Nitrogen sources are usually organic or inorganic nitrogencompounds or materials containing said compounds. Examples of nitrogensources include ammonia gas, aqueous ammonia solutions or ammonium saltssuch as NH₄Cl, or (NH₄)₂SO₄, NH₄OH, nitrates, urea, amino acids orcomplex nitrogen sources such as cornsteep liquor, soybean flour,soybean protein, yeast extract, meat extract and others. Mixtures of theabove nitrogen sources may be used advantageously.

Inorganic salt compounds, which may be included in the media comprisethe chloride, phosphorus or sulfate salts of calcium, magnesium, sodium,cobalt, molybdenum, potassium, manganese, zinc, copper and iron.Chelating agents may be added to the medium in order to keep the metalions in solution. Particularly suitable chelating agents includedihydroxyphenols such as catechol or protocatechulate or organic acidssuch as citric acid. The media usually also contain other growth factorssuch as vitamins or growth promoters, which include, for example,biotin, riboflavin, thiamine, folic acid, nicotinic acid, panthothenateand pyridoxine. Growth factors and salts are frequently derived fromcomplex media components such as yeast extract, molasses, cornsteepliquor and the like. The exact composition of the compounds used in themedia depends heavily on the particular experiment and is decided uponindividually for each specific case. Information on the optimization ofmedia can be found in the textbook “Applied Microbiol. Physiology, APractical Approach” (Ed. P. M. Rhodes, P. F. Stanbury, IRL Press (1997)S. 53-73, ISBN 0 19 963577 3). Growth media can also be obtained fromcommercial suppliers, for example Standard 1 (Merck) or BHI (Brain heartinfusion, DIFCO) and the like.

All media components are sterilized, either by heat (20 min at 1.5 barund 121° C.) or by filter sterilization. The components may besterilized either together or, if required, separately. All mediacomponents may be present at the start of the cultivation or addedcontinuously or batchwise, as desired.

The culture conditions are defined separately for each experiment. Thetemperature is normally between 15° C. and 45° C. and may be keptconstant or may be altered during the experiment. The pH of the mediumshould be in the range from 5 to 8.5, preferably around 7.0, and can bemaintained by adding buffers to the media. An example of a buffer forthis purpose is a potassium phosphate buffer. Synthetic buffers such asMOPS, HEPES, ACES and the like may be used as an alternative orsimultaneously. The culture pH value may also be kept constant duringthe culture period by addition of, for example, NaOH or NH₄OH. Ifcomplex media components such as yeast extract are used, additionalbuffers are required less since many complex compounds have a highbuffer capacity. When using a fermenter for the culture ofmicroorganisms, the pH value can also be regulated using gaseousammonia.

The incubation period is generally in a range of from several hours toseveral days. This time period is selected in such a way that themaximum amount of product accumulates in the fermentation broth. Thegrowth experiments, which are disclosed, can be carried out in amultiplicity of containers such as microtiter plates, glass tubes, glassflasks or glass or metal fermenters of various sizes. To screen a largenumber of clones, the microorganisms should be grown in microtiterplates, glass tubes or shake flasks, either using simple flasks orbaffle flasks. 100 ml shake flasks filled with 10% (based on the volume)of the growth medium required are preferably used. The flasks should beshaken on an orbital shaker (amplitude 25 mm) at a rate ranging from 100to 300 rpm. Evaporation lasses can be reduced by maintaining a humidatmosphere; as an alternative, a mathematical correction should becarried out for the evaporation losses.

If genetically modified clones are examined, an unmodified controlclone, or a control clone, which contains the basic plasmid withoutinsertion, should also be included in the tests. If a transgenicsequence is expressed, a control clone should advantageously again beincluded in these tests. The medium is advantageously inoculated to anOD600 of 0.5 to 1.5 using cells which have been grown on agar plates,such as CM plates (10 g/l glucose, 2.5 g/l NaCl, 2g/l urea, 10 g/lpolypeptone, 5 g/l yeast extract, 5 g/1 meat extract, 22g/l agar, pHvalue 6.8 established with 2M NaOH), which have been incubated at 30° C.The media are inoculated either by introducing a saline solution of C.glutamicum cells from CM plates or by addition of a liquid preculture ofthis bacterium.

Example 7 In-Vitro Analysis of the Function of the Proteins Encoded bythe Transformed Sequences

The determination of the activities and kinetic parameters of enzymes iswell known in the art. Experiments for determining the activity of aspecific modified enzyme must be adapted to the specific activity of thewild-enzyme type, which is well within the capabilities of the skilledworker. Overviews of enzymes in general and specific details regardingthe structure, kinetics, principles, methods, applications and examplesfor the determination of many enzyme activities can be found for examplein the following literature: Dixon, M., and Webb, E. C: (1979) Enzymes,Longmans, London; Fersht (1985) Enzyme Structure and Mechanism, Freeman,N.Y.; Walsh (1979) Enzymatic Reaction Mechanisms. Freeman, SanFrancisco; Price, N. C., Stevens, L. (1982) Fundamentals of Enzymology.Oxford Univ. Press: Oxford; Boyer, P. D: Ed. (1983) The Enzymes, 3rd Ed.Academic Press, New York; Bisswanger, H. (1994) Enzymkinetik, 2nd Ed.VCH, Weinheim (ISBN 3527300325); Bergmeyer, H. U., Bergmeyer, J., Graβl,M. Ed. (1983-1986) Methods of Enzymatic Analysis, 3^(rd) Ed. Vol. I-XII,Verlag Chemie: Weinheim; and Ullmann's Encyclopedia of IndustrialChemistry (1987) Vol. A9, “Enzymes”, VCH, Weinheim, pp. 352-363.

Example 8 Analysis of the Effect of the Nucleic Acid Molecule on theProduction of the Amino Acids

The effect of the genetic modification in C. glutamicum on theproduction of an amino acid can be determined by growing the modifiedmicroorganisms under suitable conditions (such as those described above)and analyzing the medium and/or the cellular components for theincreased production of the amino acid. Such analytical techniques arewell known to the skilled worker and encompass spectroscopy, thin-layerchromatography, various types of staining methods, enzymatic andmicrobiological methods and analytical chromatography such ashigh-performance liquid chromatography (see, for example, Ullman,Encyclopedia of Industrial Chemistry, Vol. A2, pp. 89-90 and pp.443-613, VCH: Weinheim (1985); Fallon, A., et al., (1987) “Applicationsof HPLC in Biochemistry” in: Laboratory Techniques in Biochemistry andMolecular Biology, Vol. 17; Rehm et al. (1993) Biotechnology, Vol. 3,Chapter III: “Product recovery and purification”, pp. 469-714, VCH:Weinheim; Belter, P. A. et al. (1988) Bioseparations: downstreamprocessing for Biotechnology, John Wiley and Sons; Kennedy, J. F. andCabral, J. M. S. (1992) Recovery processes for biological Materials,John Wiley and Sons; Shaeivitz, J. A. and Henry, J. D. (1988)Biochemical Separations, in Ullmann's Encyclopedia of IndustrialChemistry, Vol. B3; chapter 11, pp. 1-27, VCH: Weinheim; and Dechow, F.J. (1989) Separation and purification techniques in biotechnology, NoyesPublications).

In addition to the determination of the fermentation end product, othercomponents of the metabolic pathways which are used for the productionof the desired compound, such as intermediates and by-products, may alsobe analyzed in order to determine the total productivity of theorganism, the yield and/or production efficiency of the compound. Theanalytical methods encompass determining the amounts of nutrients in themedium (for example sugars, hydrocarbons, nitrogen sources, phosphateand other ions), determining biomass composition and growth, analyzingthe production of ordinary metabolites from biosynthetic pathways andmeasuring gases generated during the fermentation. Standard methods forthese are described in Applied Microbial Physiology; A PracticalApproach, P. M. Rhodes and P. F. Stanbury, Ed. IRL Press, pp. 103-129;131-163 and 165-192 (ISBN: 0199635773) and the references cited therein.

Example 9 Purification of the Amino Acid

The amino acid can be recovered from cells or from the supernatant ofthe above-described culture by a variety of methods known in the art.For example, the culture supernatant is recovered first. To this end,the cells are harvested from the culture by slow centrifugation. Cellscan generally be disrupted or lysed by standard techniques such asmechanical force or sonication. The cell debris is removed bycentrifugation and the supernatant fraction, if appropriate togetherwith the culture supernatant, is used for the further purification ofthe amino acid. However, it is also possible to process the supernatantalone if the amino acid is present in the supernatant in sufficientlyhigh a concentration. In this case, the amino acid, or the amino acidmixture, can be purified further for example via extraction and/or saltprecipitation or via ion-exchange chromatography.

If required and desired, further chromatography steps with a suitableresin may follow, the amino acid, but not many contaminants in thesample, being retained on the chromatography resin or the contaminants,but not the sample with the product (amino acid), being retained on theresin. If necessary, these chromatography steps may be repeated, usingidentical or other chromatography resins. The skilled worker is familiarwith the selection of suitable chromatography resin and the mosteffective use for a particular molecule to be purified. The purifiedproduct can be concentrated by filtration or ultrafiltration and storedat a temperature at which maximum product stability is ensured. Manypurification methods, which are not limited to the above purificationmethod are known in the art. They are described, for example, in Bailey,J. E. & Ollis, D. F. Biochemical Engineering Fundamentals, McGraw-Hill:New York (1986).

Identity and purity of the amino acid isolated can be determined bystandard techniques of the art. They encompass high-performance liquidchromatography (HPLC), spectroscopic methods, mass spectrometry (MS),staining methods, thin-layer chromatography, NIRS, enzyme assay ormicrobiological assays. These analytical methods are compiled in: Pateket al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al.(1996) Biotekhnologiya 11: 27-32; and Schmidt et al. (1998) BioprocessEngineer. 19: 67-70. Ulmann's Encyclopedia of Industrial Chemistry(1996) Vol. A27, VCH: Weinheim, pp. 89-90, pp. 521-540, pp. 540-547, pp.559-566, 575-581 and pp. 581-587; Michal, G (1999) Biochemical Pathways:An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons;Fallon, A. et al. (1987) Applications of HPLC in Biochemistry in:Laboratory Techniques in Biochemistry and Molecular Biology, Vol. 17.

Example 10 Engineering of Arabidopsis Plants by Inactivation orDown-Regulation of Genes

A binary knock out vector was constructed based on the modified pPZPbinary vector backbone (comprising the kanamycin-gene for bacterialselection; Hajdukiewicz, P. et al., 1994, Plant Mol. Biol., 25: 999-994)and the selection marker bar-gene (De Block et al., 1987, EMBO J. 6,2513-2518) driven by the mas2′1′ and mas271f promoters (Velten et al.,1984, EMBO J. 3, 2723-2730; Mengiste, Amedeo and Paszkowski, 1997, PlantJ., 12, 945-948).

Examples of other usable binary vectors for insertional mutagenesis arepBIN19, pBI101, pBinAR, pSun or pGPTV. An overview over binary vectorsand their specific features is given in Hellens et al., 2000, Trends inplant Science, 5:446-451 and in Guerineau F., Mullineaux P., 1993, Planttransformation and expression vectors in plant molecular biology, LABFAXSeries, (Croy R. R. D., ed.) pp. 121-127 Bios Scientific Publishers,Oxford.

Transformation of Agrobacteria

The plasmid was transformed into Agrobacterium tumefaciens (GV3101pMP90;Koncz and Schell, 1986 Mol. Gen. Genet. 204:383-396) using heat shock orelectroporation protocols. Transformed colonies were grown on YEB mediumand selected by respective antibiotics (Rif/Gent/Km) for 2 d at 28° C.These agrobacteria cultures were used for the plant transformation.

Arabidopsis thaliana of the ecotype C24 were grown and transformedaccording to standard conditions (Bechtold, N., Ellis, J., Pelletier, G.1993. In planta Agrobacterium mediated gene transfer by infiltration ofArabidopsis thaliana plants, C.R. Acad. Sci. Paris 316:1194-1199; Bent,A. F., Clough, J. C., 1998; Floral dip: a simplified method forAgrobacterium-mediated transformation of Arabidopsis thaliana, PLANT J.16:735-743).

Transformed plants (F1) were selected by the use of their respectiveresistance marker. In case of BASTA®-resistance, plantlets were sprayedfour times at an interval of 2 to 3 days with 0.02% BASTA® andtransformed plants were allowed to set seeds. 50-100 seedlings (F2) weresubjected again to marker selection, in case of BASTA-resistance byspaying with 0.1% BASTA® on 4 consecutive days during the plantletphase. Plants segregating for a single resistance locus (approximately3:1 resistant seedling to sensitive seedlings) were chosen for furtheranalysis. From these lines three of the resistant seedlings (F2) wereagain allowed to set seeds and were tested for homozygosis throughin-vitro germination of their seeds (F3) on agar medium containing theselection agent (BASTA®, 15 mg/L ammonium glufosinate, Pestanal, Riedelde Haen, Seeize, Germany). Those F2 lines which showed nearly 100%resistant offspring (F3) were considered homozygote and taken forfunctional analysis.

Example 11 Plant Culture for Bioanalytical Analyses

For the bioanalytical analyses of the transgenic plants, the latter weregrown uniformly a specific culture facility. To this end the GS-90substrate as the compost mixture was introduced into the potting machine(Laible System GmbH, Singen, Germany) and filled into the pots.Thereafter, 35 pots were combined in one dish and treated with Previcur.For the treatment, 25 ml of Previcur were taken up in 10 l of tap water.This amount was sufficient for the treatment of approximately 200 pots.The pots were placed into the Previcur solution and additionallyirrigated overhead with tap water without Previcur. They were usedwithin four days.

For the sowing, the seeds, which had been stored in the refrigerator (at−20° C.), were removed from the Eppendorf tubes with the aid of atoothpick and transferred into the pots with the compost. In total,approximately 5 to 12 seeds were distributed in the middle of the pot.

After the seeds had been sown, the dishes with the pots were coveredwith matching plastic hood and placed into the stratification chamberfor 4 days in the dark at 4° C. The humidity was approximately 90%.After the stratification, the test plants were grown for 22 to 23 daysat a 16-h-light, 8-h-dark rhythm at 20° C., an atmospheric humidity of60% and a CO₂ concentration of approximately 400 ppm. The light sourcesused were Powerstar HQI-T 250 W/D Daylight lamps from Osram, whichgenerate a light resembling the solar color spectrum with a lightintensity of approximately 220 μE/m²/s⁻¹.

The resistance plants were thinned when they had reached the age of 14days. The plants, which had grown best in the center of the pot wereconsidered the target plants. All the remaining plants were removedcarefully with the aid of metal tweezers and discarded.

During their growth, the plants received overhead irrigation withdistilled water (onto the compost) and bottom irrigation into theplacement grooves. Once the grown plants had reached the age of 23 days,they were harvested.

Example 12 Metabolic Analysis of Transformed Plants

The modifications identified in accordance with the invention, in thecontent of above-described metabolites, were identified by the followingprocedure.

a) Sampling and Storage of the Samples

Sampling was performed directly in the controlled-environment chamber.The plants were cut using small laboratory scissors, rapidly weighed onlaboratory scales, transferred into a pre-cooled extraction sleeve andplaced into an aluminum rack cooled by liquid nitrogen. If required, theextraction sleeves can be stored in the freezer at −80° C. The timeelapsing between cutting the plant to freezing it in liquid nitrogenamounted to not more than 10 to 20 seconds.

b) Lyophilization

During the experiment, care was taken that the plants either remained inthe deep-frozen state (temperatures <−40° C.) or were freed from waterby lyophilization until the first contact with solvents.

The aluminum rack with the plant samples in the extraction sleeves wasplaced into the pre-cooled (−40° C.) lyophilization facility. Theinitial temperature during the main drying phase was −35° C. and thepressure was 0.120 mbar. During the drying phase, the parameters werealtered following a pressure and temperature program. The finaltemperature after 12 hours was +30° C. and the final pressure was 0.001to 0.004 mbar. After the vacuum pump and the refrigerating machine hadbeen switched off, the system was flushed with air (dried via a dryingtube) or argon.

c) Extraction

Immediately after the lyophilization apparatus had been flushed, theextraction sleeves with the lyophilized plant material were transferredinto the 5 ml extraction cartridges of the ASE device (AcceleratedSolvent Extractor ASE 200 with Solvent Controller and AutoASE software(DIONEX)).

The 24 sample positions of an ASE device (Accelerated Solvent ExtractorASE 200 with Solvent Controller and AutoASE software (DIONEX)) werefilled with plant samples, including some samples for testing qualitycontrol.

The polar substances were extracted with approximately 10 ml ofmethanol/water (80/20, v/v) at T=70° C. and p=140 bar, 5 minutesheating-up phase, 1 minute static extraction. The more lipophilicsubstances were extracted with approximately 10 ml ofmethanol/dichloromethane (40/60, v/v) at T=70° C. and p=140 bar, 5minute heating-up phase, 1 minute static extraction. The two solventmixtures were extracted into the same glass tubes (centrifuge tubes, 50ml, equipped with screw cap and pierceable septum for the ASE (DIONEX)).

The solution was treated with internal standards: ribitol,L-glycine-2,2-d₂, L-alanine-2,3,3,3-d₄, methionine-methyl-d₃, andα-methylglucopyranoside and methyl nonadecanoate, methyl undecanoate,methyl tridecanoate, methyl pentadecanoate, methyl nonacosanoate.

The total extract was treated with 8 ml of water. The solid residue ofthe plant sample and the extraction sleeve were discarded.

The extract was shaken and then centrifuged for 5 to 10 minutes at least1 400 g in order to accelerate phase separation. 1 ml of the supernatantmethanol/water phase (“polar phase”, colorless) was removed for thefurther GC analysis, and 1 ml was removed for the LC analysis. Theremainder of the methanol/water phase was discarded. 0.5 ml of theorganic phase (“lipid phase”, dark green) was removed for the further GCanalysis and 0.5 ml was removed for the LC analysis. All the portionsremoved were evaporated to dryness using the IR Dancer infrared vacuumevaporator (Hettich). The maximum temperature during the evaporationprocess did not exceed 40° C. Pressure in the apparatus was not lessthan 10 mbar.

d) Processing the Lipid Phase for the LC/MS or LC/MS/MS Analysis

The lipid extract, which had been evaporated to dryness was taken up inmobile phase. The HPLC was run with gradient elution.

The polar extract, which had been evaporated to dryness was taken up inmobile phase. The HPLC was run with gradient elution.

e) Derivatization of the Lipid Phase for the GC/MS Analysis

For the transmethanolysis, a mixture of 140 μl of chloroform, 37 μl ofhydrochloric acid (37% by weight HCl in water), 320 μl of methanol and20 μl of toluene was added to the evaporated extract. The vessel wassealed tightly and heated for 2 hours at 100° C., with shaking. Thesolution was subsequently evaporated to dryness. The residue was driedcompletely.

The methoximation of the carbonyl groups was carried out by reactionwith methoxyamine hydrochloride (5 mg/ml in pyridine, 100 μl for 1.5hours at 60° C.) in a tightly sealed vessel. 20 μl of a solution ofodd-numbered, straight-chain fatty acids (solution of each 0.3 mg/mL offatty acids from 7 to 25 carbon atoms and each 0.6 mg/mL of fatty acidswith 27, 29 and 31 carbon atoms in 3/7 (v/v) pyridine/toluene) wereadded as time standards. Finally, the derivatization with 100 μl ofN-methyl-N-(trimethylsilyl)-2,2,2-trifluoroacetamide (MSTFA) was carriedout for 30 minutes at 60° C., again in the tightly sealed vessel. Thefinal volume before injection into the GC was 220 μl.

f) Analysis of the Various Plant Samples

The samples were measured in individual series of 20 plant samples each(also referred to as sequences). In screening experiments 20 differenttransgenic lines were analysed per sequence while in confirmationexperiments each sequence contained at least 3 replicates per transgeniclines plus at least 5 wild-type plants as controls. The peak areas foreach analyte were adjusted for the fresh weight established for theplant (normalized area). Ratio values were calculated by furthernormalization to the control. In screening experiments, this control wasthe median value calculated for a given analyte based on all normalizedareas of all plants in the corresponding sequence. In confirmationexperiments ratio values were calculated by dividing the normalized areaby the mean of the corresponding data of the wild-type control group ofthe same sequence. The values obtained are referred to asratio_by_control. They are comparable between sequences and indicate howmuch the analyte concentration in the mutant differs from the controlgroup, which is either the wild type control (confirmation experiments)or the rest of the mutants (screening) of a given sequence. Appropriatecontrols were done before to proof that the vector and transformationprocedure itself has no significant influence on the metaboliccomposition of the plants. Therefore the described changes in comparisonwith the control group were undoubtedly caused by the mutation.

As an alternative, the amino acids can be detected advantageously viaHPLC separation in ethanolic extract as described by Geigenberger et al.(Plant Cell & Environ, 19, 1996: 43-55).

The results of the different plant analyses can be seen from thefollowing table 2:

TABLE 2 Results of the analysis of the compound of the invention Min andMax seqID Accession Metabolite Ratio by WT Method 1 At2g25320 Methionine1.11-2.02 LC 7 At3g07610 Methionine 1.49-2.77 LC 17 At3g15600 Methionine1.08-1.77 LC 64 At1g14490 Methionine 1.01-1.83 LC 151 At1g30570Methionine 1.06-2.13 GC 228 At2g21290 Methionine 1.14-1.35 LC 282At3g14230 Methionine 1.22-2.19 LC

Column 1 shows the seqID of the gene, which has been knocked out, column2 display the respective genebank accession number. Column 3 shows theamino acid analyzed. Column 4 shows the ratio of the analyzed amino acidbetween the transgenic plants and the wild type. Column 5 indicates theanalytical method.

When the analyses were repeated independently, all results proved to besignificant.

Analysis of the Selected Metabolically Changed Lines

Since the lines were preselected for single insertion loci and ahomozygous situation of the resistance marker, the disruption (ormutation) of single genes through the integration of the T-DNA wereexpected to have lead to the metabolic phenotype. Lines which showed aconsistent phenotype were chosen for molecular analysis.

Genomic DNA was purified from approximately 100 mg of leaf tissue fromthese lines using standard procedures (either spins columns from Qiagen,Hilden, Germany or the Nucleon Phytopure Kit from Amersham Biosciences,Freiburg, Germany). The amplification of the insertion side of the T-DNAwas achieved using two different methods. Either by an adaptorPCR-method according to Spertini D, Béliveau C. and Bellemare G., 1999,Biotechniques, 27, 308-314 using T-DNA specific primers

-   LB1 (5′-TGA CGC CAT TTC GCC TTT TCA-3′; SEQ ID NO: 35) or-   RB 1-2 (5′-CAA CTT AAT CGC CTT GCA GCA CA-3′; SEQ ID NO: 36) for the    first and-   LB2 (5′-CAG AAA TGG ATA MT AGC CTT GCT TCC-3′; SEQ ID NO: 37) or-   RB4-2 (5′-AGC TGG CGT AAT AGC GAA GAG-3′; SEQ ID NO: 38) for the    second PCR respectively. Alternatively TAIL-PCR (Liu Y-G, Mitsukawa    N, Oosumi T and Whittier R F, 1995, Plant J. 8, 457-463) was    performed. In this case for the first PCR-   LB1 (5′-TGA CGC CAT TTC GCC TTT TCA-3′, SEQ ID NO: 35) or-   RB1-2 (5′-CAA CTT AAT CGC CTT GCA GCA CA-3′; SEQ ID NO: 36), for the    second PCR-   LB2 (5′-CAG AAA TGG ATA AAT AGC CTT GCT TCC-3′; SEQ ID NO: 37) or    RB4-2 (5′-AGC TGG CGT AAT AGC GAA GAG-3′, SEQ ID NO: 38) and for the    last PCR-   LB3 (5′-CCA ATA CAT TAC ACT AGC ATC TG-3′; SEQ ID NO: 39) or-   RB5 (5′-AAT GCT AGA GCA GCT TGA-3′; SEQ ID NO: 40) were used as    T-DNA specific primers for left or right T-DNA borders respectively.

Appropriate PCR-products were identified on agarose gels and purifiedusing columns and standard procedures (Qiagen, Hilden, Germany).PCR-products were sequenced with additional T-DNA-specific primerslocated towards the borders relative to the primers used foramplification. For adaptor PCR products containing left border sequencesprimer

-   LBseq (5′-CAA TAC ATT ACA CTA GCA TCT G-3′; SEQ ID NO: 41) and for    sequences containing right border sequences primer-   RBseq (5′-AGA GGC CCG CAC CGA TCG-3′; SEQ ID NO: 42) were used for    sequencing reactions. For TAIL PCR products containing left border    sequences primer-   Lbseq2 (5′-CTA GCA TCT GAA TTT CAT AAC C-3′; SEQ ID NO: 43) and for    PCR products containing right border sequences primer-   Rbseq2 (5′-GCT TGA GCT TGG ATC AGA TTG-3′; SEQ ID NO: 44) were used    for sequencing reactions. The resulting sequences were taken for    comparison with the available Arabidopsis genome sequence from    Genbank using the blast algorithm (Altschul et al., 1990. J Mol    Biol, 215:403-410).

Details on PCR products used to identify the genomic locus are given intable 3. Indicated are the identified annotated open reading frame inthe Arabidopsis genome, the estimated size of the obtained PCR product(in base pairs), the T-DNA border (LB: left border, RB: right border)for which the amplification was achieved, the method which resulted inthe indicated PCR product (explanation see text above), the respectiverestriction enzymes in case of adaptor PCR, and the degenerated primerin the case of TAIL PCR. Routinely degenerated primers

-   ADP2 (5′-NGT CGA SWG ANA WGA A-3′; SEQ ID NO: 45),-   ADP3 (5′-WGTGNAGWANCANAGA-3′; SEQ ID NO: 46),-   ADP5 (5′-STT GNT AST NCT NTG C-3′; SEQ ID NO: 47),-   ADP6 (5′-AGWGNAGWANCANAGA-3′; SEQ ID NO: 48),-   ADP8 (5′-NTG CGA SWG ANT AGA A-3′; SEQ ID NO: 49),-   ADP9 (5′-NTG CGA SWG ANT AGA A-3′; SEQ ID NO: 50) and-   ADP11 (5′-SST GGS TAN ATW ATW CT-3′; SEQ ID NO: 51) were used.

The identification of the insertion locus in each case was confirmed bya control PCR, using one of the above mentioned T-DNA-specific primersand a primer deduced from the identified genomic locus, near to theinsertion side. The amplification of a PCR-product of the expected sizefrom the insertion line using these two primers proved the disruption ofthe identified locus by the T-DNA integration.

Table 3: Details on PCR products used to identify the down-regulatedgenomic locus in lines showing increased Methionine.

TABLE 3 PCR-products PCR- Restriction enzyme SEQ ID ORF Product BorderMethod or deg. primer 1 at2g25320 1000 RB Adapter Mun I 7 at3g07610 820LB Adapter Spe I 17 at3g15600 750 LB Adapter Psp1406 I/Bsp119 I 64At1g14490 550 LB Adapter SpeI 151 At1g30570 250 LB AdapterPsp1406/Bsp119I 228 At2g21290 350 LB Adapter SpeI 282 At3g14230 300 LBAdapter BgIIIColumn 1 refers to the SEQ ID of the gene which has been knocked out,column 2 refers to the genebank accession of the gene, column 3 refersto the approximate length of the amplified PCR product, column 4 refersto the T-DNA border for which the PCR product was amplified, column 5refers to the PCR method for amplification and column 6 refers torestriction enzyme of degenerate primer used in the PRC method (fordetailed examplation to columns 5 and 6 see text above)

Example 13 Construction of Antisense Constructs for Repression of theGenes of the Invention

A fragment of SEQ ID NO: 7 is amplified by PCR. To enable cloning of thePCR product, restriction sites may be added to the primers used for theamplification. Alternatively recombination sites may be added to theprimers to enable a recombination reaction. The PCR fragment is eithercloned or recombined into a binary vector, preferently under control ofa strong constitutive, tissue or developmental specific promoters in away, that the orientation to the promoter is opposite of the directionthe genes has in its original genomic position.

The amplification of the fragment of the SEQ ID NO: 7 was performedusing the oligonucleotides that have been deduced from the genesequence:

(SEQ ID NO: 52) a3g07610fw: 5′-atattaattaaggtaattaggtatcatatct-3′ (SEQID NO: 53) a3g07610rev: 5′-ataccatggggatctacgtaattcgccag-3′

The Oligonucleotides have been solved in water to give a concentrationof 20 μM. The PCR reaction contained 5 μl Herculase buffer (Stratagene),0.4 μl dNTPs (25 mM each) (Amersham), 0.5 μl Primer a07610fw, 0.5 μlPrimer a07610rev, 0.5 μl Herculase (Stratagene), 0.5 μl gDNA and 42.6 μlwater. The PCR was performed on MJ-Cycler Tetrad (BioZym) with thefollowing program:

4 min 94° C., followed by 30 cycles of 1 min 94° C., 1 min 50° C., 2 min72° C. followed by 10 min 72° C. and cooling to 25° C.

The PCR product has been purified using a Kit from Qiagen. The DNA wassubsequently digested with NcoI/PacI at 37° C. over night. The fragmentwas then cloned into the vector 1bxPcUbicolic which has been digestedwith NcoI/PacI. The resulting construct was named 1bxPcUbianti3g07610

Example 14 Construction of RNAi Constructs for Repression of the Genesof the Invention

A fragment of SEQ ID NO: 7 is amplified by PCR. To enable cloning of thePCR product, restriction sites may be added to the primers used for theamplification. Alternatively recombination sites may be added to theprimers to enable a recombination reaction. The PCR fragment is eithercloned or recombined into a binary vector, preferently under control ofa strong constitutive, tissue or developmental specific promoters in away, that the fragment is introduced twice in the vector as an invertedrepeat, the repeats separated by a DNA spacer.

The amplification of the fragment of the SEQ ID NO: 7 was performedusing the oligonucleotides that have been deduced from the genesequence:

(SEQ ID NO: 54) ri3g07610fw: 5′-ataggtaccggtaattaggtatcatatct-3′ (SEQ IDNO: 55) ri3g07610rev: 5′-atagtcgacggatctacgtaattcgccag-3′

The Oligonucleotides have been solved in water to give a concentrationof 20 μM. The PCR reaction contained 5 μl Herculase buffer (Stratagene),0.4 μl dNTPs (25 mM each) (Amersham), 0.5 μl Primer a07610fw, 0.5 μlPrimer a07610rev, 0.5 μl Herculase (Stratagene), 0.5 μl gDNA and 42.6 μlwater. The PCR was performed on MJ-Cycler Tetrad (BioZym) with thefollowing program:

4 min 94° C., followed by 30 cycles of 1 min 94° C., 1 min 50° C., 2 min72° C. followed by 10 min 72° C. and cooling to 25° C.

The PCR product has been purified using a Kit from Qiagen. The DNA wassubsequently digested with Asp718/SalI at 37° C. over night. Thefragment was then cloned into the vector 10×PcUbispacer which has beendigested with Asp718/SalI. The resulting construct was digested withXhoI/BsrGI and the same Asp718/SalI digested PCR fragment was ligatedinto this vector. Subsequently, the expression cassette giving rise toBASTA resistance was ligated as XbaI fragment into this vector that hasbeen opened with XbaI and dephosphorilized before. The resultingconstruct was named 1bxPcUbiri3g07610

Example 15 Construction of Cosuppression Constructs for Repression ofthe Genes of the Invention

A fragment of SEQ ID NO: 7 is amplified by PCR. To enable cloning of thePCR product, restriction sites may be added to the primers used for theamplification. Alternatively recombination sites may be added to theprimers to enable a recombination reaction. The PCR fragment is eithercloned or recombined into a binary vector, preferently under control ofa strong constitutive, tissue or developmental specific promoters in away, that the orientation to the promoter is identical to the directionthe gene has in its original genomic position.

The amplification of the fragment of the SEQ ID NO: 7 was performedusing the oligonucleotides that have been deduced from the genesequence:

(SEQ ID NO: 56) c3g07610fw: 5′-ataccatggggtaattaggtatcatatct-3′ (SEQ IDNO: 57) c3g07610rev: 5′-atattaattaaggatctacgtaattcgccag-3′

The Oligonucleotides have been solved in water to give a concentrationof 20 μM. The PCR reaction contained 5 μl Herculase buffer (Stratagene),0.4 μl dNTPs (25 mM each) (Amersham), 0.5 μl Primer a07610fw, 0.5 μlPrimer a07610rev, 0.5 μl Herculase (Stratagene), 0.5 μl gDNA and 42.6 μlwater. The PCR was performed on MJ-Cycler Tetrad (BioZym) with thefollowing program:

4 min 94° C., followed by 30 cycles of 1 min 94° C., 1 min 50° C., 2 min72° C. followed by 10 min 72° C. and cooling to 25° C.

The PCR product has been purified using a Kit from Qiagen. The DNA wassubsequently digested with NcoI/PacI at 37° C. over night. The fragmentwas then cloned into the vector 1bxPcUbicolic which has been digestedwith NcoI/PacI. The resulting construct was named 1bxPcUbicos3g07610.

Example 16 Reducing the Expression of the Genes of the Invention byArtificial Transcription Factors

The genes of the invention and their homologous ORFs in other speciesmay also be down regulated by introducing a synthetic specificrepressor. For this purpose, a gene for a chimeric zinc finger protein,which binds to a specific region in the regulatory or coding region ofthe genes of interests or its homologs in other species is constructed.The artificial zinc finger protein comprises a specific DNA-bindingdomain consting for example of zinc finger and optional an repressionlike the EAR domain (Hiratsu et al., 2003. Plant J. 34(5), 733-739Dominant repression of target genes by chimeric repressors that includethe EAR motif, a repression domain, in Arabidopsis.)

Expression of this chimeric repressor for example in plants then resultsin specific repression of the target gene or of its homologs in otherplant species lead to increased metabolite production The experimentaldetails especially about the design and construction of specific zincfinger domains may be carried out as described, or WO 01/52620 or OrdizM I, (Proc. Natl. Acad. Sci. USA, 2002, Vol. 99, Issue 20, 13290) orGuan, (Proc. Natl. Acad. Sci. USA, 2002, Vol. 99, Issue 20, 13296).

Example 17 Engineering Ryegrass Plants by Repressing the Nucleic AcidSequence Homologous of the Invention in Ryegrass

Seeds of several different ryegrass varieties can be used as explantsources for transformation, including the commercial variety Gunneavailable from Svalof Weibull Seed Company or the variety Affinity.Seeds are surface-sterilized sequentially with 1% Tween-20 for 1 minute,100% bleach for 60 minutes, 3 rinses with 5 minutes each with de-ionizedand distilled H₂O, and then germinated for 3-4 days on moist, sterilefilter paper in the dark. Seedlings are further sterilized for 1 minutewith 1% Tween-20, 5 minutes with 75% bleach, and rinsed 3 times withddH2O, 5 min each.

Surface-sterilized seeds are placed on the callus induction mediumcontaining Murashige and Skoog basal salts and vitamins, 20 g/l sucrose,150 mg/l asparagine, 500 mg/l casein hydrolysate, 3g/l Phytagel, 10 mg/lBAP, and 5 mg/l dicamba. Plates are incubated in the dark at 25° C. for4 weeks for seed germination and embryogenic callus induction.

After 4 weeks on the callus induction medium, the shoots and roots ofthe seedlings are trimmed away, the callus is transferred to freshmedia, is maintained in culture for another 4 weeks, and is thentransferred to MSO medium in light for 2 weeks. Several pieces of callus(11-17 weeks old) are either strained through a 10 mesh sieve and putonto callus induction medium, or are cultured in 100 ml of liquidryegrass callus induction media (same medium as for callus inductionwith agar) in a 250 ml flask. The flask is wrapped in foil and shaken at175 rpm in the dark at 23° C. for 1 week. Sieving the liquid culturewith a 40-mesh sieve is collected the cells. The fraction collected onthe sieve is plated and is cultured on solid ryegrass callus inductionmedium for 1 week in the dark at 25° C. The callus is then transferredto and is cultured on MS medium containing 1% sucrose for 2 weeks.

Transformation can be accomplished with either Agrobacterium or withparticle bombardment methods. An expression vector is created containinga constitutive plant promoter and the repression construct of the genein a pUC vector. The plasmid DNA is prepared from E. coli cells usingwith Qiagen kit according to manufacturer's instruction. Approximately 2g of embryogenic callus is spread in the center of a sterile filterpaper in a Petri dish. An aliquot of liquid MSO with 10 g/l sucrose isadded to the filter paper. Gold particles (1.0 μm in size) are coatedwith plasmid DNA according to method of Sanford et al., 1993 and aredelivered to the embryogenic callus with the following parameters: 500μg particles and 2 μg DNA per shot, 1300 psi and a target distance of8.5 cm from stopping plate to plate of callus and 1 shot per plate ofcallus.

After the bombardment, calli are transferred back to the fresh callusdevelopment medium and maintained in the dark at room temperature for a1-week period. The callus is then transferred to growth conditions inthe light at 25° C. to initiate embryo differentiation with theappropriate selection agent, e.g. 250 nM Arsenal, 5 mg/l PPT or 50 mg/LKanamycin. Shoots resistant to the selection agent are appearing andonce rooted are transferred to soil.

Samples of the primary transgenic plants (T0) are analyzed by PCR toconfirm the presence of T-DNA. These results are confirmed by Southernhybridization in which DNA is electrophoresed on a 1% agarose gel andtransferred to a positively charged nylon membrane (Roche Diagnostics).The PCR DIG Probe Synthesis Kit (Roche Diagnostics) is used to prepare adigoxigenin-labelled probe by PCR, and used as recommended by themanufacturer.

Transgenic T0 ryegrass plants are propagated vegetatively by excisingtillers. The transplanted tillers are maintained in the greenhouse for 2months until well established. The shoots are defoliated and allowed togrow for 2 weeks.

Example 18 Engineering Soybean Plants by Repressing the Nucleic AcidSequence Homologous of the Invention in Soybean

Soybean can be transformed according to the following modification ofthe method described in the Texas A&M U.S. Pat. No. 5,164,310. Severalcommercial soybean varieties are amenable to transformation by thismethod. The cultivar Jack (available from the Illinois Seed Foundation)is commonly used for transformation. Seeds are sterilized by immersionin 70% (v/v) ethanol for 6 min and in 25% commercial bleach (NaOCl)supplemented with 0.1% (v/v) Tween for 20 min, followed by rinsing 4times with sterile double distilled water. Removing the radicle,hypocotyl and one cotyledon from each seedling propagates seven-dayseedlings. Then, the epicotyl with one cotyledon is transferred to freshgermination media in petri dishes and incubated at 25° C. under a 16-hrphotoperiod (approx. 100 μE-m-2s-1) for three weeks. Axillary nodes(approx. 4 mm in length) are cut from 3 to 4 week-old plants. Axillarynodes are excised and incubated in Agrobacterium LBA4404 culture.

Many different binary vector systems have been described for planttransformation (e.g. An, G. in Agrobacterium Protocols. Methods inMolecular Biology vol 44, pp 47-62, Gartland KMA and MR Davey eds.Humana Press, Totowa, N.J.). Many are based on the vector pBIN19described by Bevan (Nucleic Acid Research. 1984. 12:8711-8721) thatincludes a plant gene expression cassette flanked by the left and rightborder sequences from the Ti plasmid of Agrobacterium tumefaciens. Aplant gene expression cassette consists of at least two genes—aselection marker gene and a plant promoter regulating the transcriptionof the repression cassette of the trait gene. Various selection markergenes can be used as described above, including the Arabidopsis geneencoding a mutated acetohydroxy acid synthase (AHAS) enzyme (U.S. Pat.Nos. 57,673,666 and 6,225,105). Similarly, various promoters can be usedto regulate the repression cassette to provide constitutive,developmental, tissue or environmental repression of gene transcriptionas described above. In this example, the 34S promoter (GenBank Accessionnumbers M59930 and X16673) is used to provide constitutive repression ofthe repression cassette.

After the co-cultivation treatment, the explants are washed andtransferred to selection media supplemented with 500 mg/L timentin.Shoots are excised and placed on a shoot elongation medium. Shootslonger than 1 cm are placed on rooting medium for two to four weeksprior to transplanting to soil.

The primary transgenic plants (T0) are analyzed by PCR to confirm thepresence of T-DNA. These results are confirmed by Southern hybridizationin which DNA is electrophoresed on a 1% agarose gel and transferred to apositively charged nylon membrane (Roche Diagnostics). The PCR DIG ProbeSynthesis Kit (Roche Diagnostics) is used to prepare adigoxigenin-labelled probe by PCR, and is used as recommended by themanufacturer.

Example 19 Engineering Corn Plants by Repressing Nucleic Acid SequenceHomologous of the Invention in Corn

Transformation of maize (Zea Mays L.) is performed with a modificationof the method described by Ishida et al. (1996. Nature Biotech14745-50). Transformation is genotype-dependent in corn and onlyspecific genotypes are amenable to transformation and regeneration. Theinbred line A188 (University of Minnesota) or hybrids with A188 as aparent are good sources of donor material for transformation (Fromm etal. 1990 Biotech 8:833-839), but other genotypes can be usedsuccessfully as well. Ears are harvested from corn plants atapproximately 11 days after pollination (DAP) when the length ofimmature embryos is about 1 to 1.2 mm. Immature embryos areco-cultivated with Agrobacterium tumefaciens that carry “super binary”vectors and transgenic plants are recovered through organogenesis. Thesuper binary vector system of Japan Tobacco is described in WO patentsWO94/00977 and WO95/06722. Vectors can be constructed as described.Various selection marker genes can be used including the maize geneencoding a mutated acetohydroxy acid synthase (AHAS) enzyme (U.S. Pat.No. 6,025,541). Similarly, various promoters can be used to regulate therepression cassette to provide constitutive, developmental, tissue orenvironmental repression of gene transcription. In this example, the 34Spromoter (GenBank Accession numbers M59930 and X16673) is used toprovide constitutive expression of the repression cassette.

Excised embryos are grown on callus induction medium, then maizeregeneration medium, containing imidazolinone as a selection agent. ThePetri plates are incubated in the light at 25° C. for 2 to 3 weeks, oruntil shoots develop. The green shoots are transferred from each embryoto maize rooting medium and incubated at 25° C. for 2 to 3 weeks, untilroots develop. The rooted shoots are transplanted to soil in thegreenhouse. T1 seeds are produced from plants that exhibit tolerance tothe imidazolinone herbicides and which are PCR positive for thetransgenes.

The T1 generation of single locus insertions of the T-DNA can segregatefor the transgene in a 3:1 ratio. Those progeny containing one or twocopies of the transgene are tolerant of the imidazolinone herbicide.Homozygous T2 plants can exhibited similar phenotypes as the T1 plants.Hybrid plants (F1 progeny) of homozygous transgenic plants andnon-transgenic plants can also exhibited increased similar phenotypes.

Example 20 Engineering Wheat Plants by Repressing Nucleic Acid SequenceHomologous of the Invention in Wheat

Transformation of wheat is performed with the method described by Ishidaet al. (1996 Nature Biotech. 14745-50). The cultivar Bobwhite (availablefrom CYMMIT, Mexico) is commonly used in transformation. Immatureembryos are cocultivated with Agrobacterium tumefaciens that carry“super binary” vectors, and transgenic plants are recovered throughorganogenesis. The super binary vector system of Japan Tobacco isdescribed in WO patents WO94/00977 and WO95/06722. Vectors wereconstructed as described. Various selection marker genes can be usedincluding the maize gene encoding a mutated acetohydroxy acid synthase(AHAS) enzyme (U.S. Pat. No. 6,025,541). Similarly, various promoterscan be used to regulate the repression cassette to provide constitutive,developmental, tissue or environmental regulation of gene repression. Inthis example, the 34S promoter (GenBank Accession numbers M59930 andX16673) can be used to provide constitutive expression of the repressioncassette.

After incubation with Agrobacterium, the embryos are grown on callusinduction medium, then regeneration medium, containing imidazolinone asa selection agent. The Petri plates are incubated in the light at 25° C.for 2 to 3 weeks, or until shoots develop. The green shoots aretransferred from each embryo to rooting medium and incubated at 25° C.for 2 to 3 weeks, until roots develop. The rooted shoots aretransplanted to soil in the greenhouse. T1 seeds are produced fromplants that exhibit tolerance to the imidazolinone herbicides and whichare PCR positive for the transgenes.

The T1 generation of single locus insertions of the T-DNA can segregatefor the transgene in a 3:1 ratio. Those progeny containing one or twocopies of the transgene are tolerant of the imidazolinone herbicide.Homozygous T2 plants exhibited similar phenotypes.

Example 21 Engineering Rapeseed/Canola Plants by Repressing Nucleic AcidSequence Homologous of the Invention in Rapeseed/Canola Plants

Cotyledonary petioles and hypocotyls of 5-6 day-old young seedlings areused as explants for tissue culture and transformed according to Babicet al. (1998, Plant Cell Rep 17: 183-188). The commercial cultivarWestar (Agriculture Canada) is the standard variety used fortransformation, but other varieties can be used.

Agrobacterium tumefaciens LBA4404 containing a binary vector are usedfor canola transformation. Many different binary vector systems havebeen described for plant transformation (e.g. An, G. in AgrobacteriumProtocols. Methods in Molecular Biology vol 44, pp 47-62, Gartland KMAand MR Davey eds. Humana Press, Totowa, N.J.). Many are based on thevector pBIN19 described by Bevan (Nucleic Acid Research. 1984.12:8711-8721) that includes a plant gene expression cassette flanked bythe left and right border sequences from the Ti plasmid of Agrobacteriumtumefaciens. A plant gene expression cassette consists of at least twogenes—a selection marker gene and a plant promoter regulating thetranscription of the repression cassette of the trait gene. Variousselection marker genes can be used including the Arabidopsis geneencoding a mutated acetohydroxy acid synthase (AHAS) enzyme (U.S. Pat.Nos. 57,673,666 and 6,225,105). Similarly, various promoters can be usedto regulate the repression cassette to provide constitutive,developmental, tissue or environmental regulation of gene repression. Inthis example, the 34S promoter (GenBank Accession numbers M59930 andX16673) can be used to provide constitutive expression of the repressioncassette.

Canola seeds are surface-sterilized in 70% ethanol for 2 min., and thenin 30% Clorox with a drop of Tween-20 for 10 min, followed by threerinses with sterilized distilled water. Seeds are then germinated invitro 5 days on half strength MS medium without hormones, 1% sucrose,0.7% Phytagar at 23° C., 16 hr. light. The cotyledon petiole explantswith the cotyledon attached are excised from the in vitro seedlings, andare inoculated with Agrobacterium by dipping the cut end of the petioleexplant into the bacterial suspension. The explants are then culturedfor 2 days on MSBAP-3 medium containing 3 mg/l BAP, 3% sucrose, 0.7%Phytagar at 23° C., 16 hr light. After two days of co-cultivation withAgrobacterium, the petiole explants are transferred to MSBAP-3 mediumcontaining 3 mg/l BAP, cefotaxime, carbenicillin, or timentin (300 mg/l)for 7 days, and then cultured on MSBAP-3 medium with cefotaxime,carbenicillin, or timentin and selection agent until shoot regeneration.When the shoots are 5 to 10 mm in length, they are cut and transferredto shoot elongation medium (MSBAP-0.5, containing 0.5 mg/l BAP). Shootsof about 2 cm in length are transferred to the rooting medium (MSO) forroot induction.

Samples of the primary transgenic plants (T0) are analyzed by PCR toconfirm the presence of T-DNA. These results are confirmed by Southernhybridization in which DNA is electrophoresed on a 1% agarose gel andare transferred to a positively charged nylon membrane (RocheDiagnostics). The PCR DIG Probe Synthesis Kit (Roche Diagnostics) isused to prepare a digoxigenin-labelled probe by PCR, and used asrecommended by the manufacturer.

Example 22 Engineering Alfalfa Plants by Repressing Nucleic AcidSequence Homologous of the Invention in Alfalfa

A regenerating clone of alfalfa (Medicago sativa) is transformed usingthe method of McKersie et al., 1999 Plant Physiol 119: 839-847.Regeneration and transformation of alfalfa is genotype dependent andtherefore a regenerating plant is required. Methods to obtainregenerating plants have been described. For example, these can beselected from the cultivar Rangelander (Agriculture Canada) or any othercommercial alfalfa variety as described by Brown DCW and A Atanassov(1985. Plant Cell Tissue Organ Culture 4: 111-112). Alternatively, theRA3 variety (University of Wisconsin) has been selected for use intissue culture (Walker et al., 1978 Am J Bot 65:654-659).

Petiole explants are cocultivated with an overnight culture ofAgrobacterium tumefaciens C58C1 pMP90 (McKersie et al., 1999 PlantPhysiol 119: 839-847) or LBA4404 containing a binary vector. Manydifferent binary vector systems have been described for planttransformation (e.g. An, G. in Agrobacterium Protocols. Methods inMolecular Biology vol 44, pp 47-62, Gartland KMA and MR Davey eds.Humana Press, Totowa, N.J.). Many are based on the vector pBIN19described by Bevan (Nucleic Acid Research. 1984. 12:8711-8721) thatincludes a plant gene expression cassette flanked by the left and rightborder sequences from the Ti plasmid of Agrobacterium tumefaciens. Aplant gene expression cassette consists of at least two genes—aselection marker gene and a plant promoter regulating the transcriptionof the repression cassette of the trait gene. Various selection markergenes can be used including the Arabidopsis gene encoding a mutatedacetohydroxy acid synthase (AHAS) enzyme (U.S. Pat. Nos. 57,673,666 and6,225,105). Similarly, various promoters can be used to regulate therepression cassette that provides constitutive, developmental, tissue orenvironmental regulation of gene repression. In this example, the 34Spromoter (GenBank Accession numbers M59930 and X16673) can be used toprovide constitutive expression of the repression cassette.

The explants are cocultivated for 3 d in the dark on SH induction mediumcontaining 288 mg/L Pro, 53 mg/L thioproline, 4.35 g/L K₂SO₄, and 100 μmacetosyringinone. The explants are washed in half-strengthMurashige-Skoog medium (Murashige and Skoog, 1962) and plated on thesame SH induction medium without acetosyringinone but with a suitableselection agent and suitable antibiotic to inhibit Agrobacterium growth.After several weeks, somatic embryos are transferred to BOi2Ydevelopment medium containing no growth regulators, no antibiotics, and50 g/L sucrose. Somatic embryos are subsequently germinated onhalf-strength Murashige-Skoog medium. Rooted seedlings are transplantedinto pots and grown in a greenhouse.

The T0 transgenic plants are propagated by node cuttings and rooted inTurface growth medium. The plants are defoliated and grown to a heightof about 10 cm (approximately 2 weeks after defoliation).

Example 23 Knock Out of the Genes of the Invention by HomologousRecombination

Identifying mutations in the genes of the invention in randommutagenized populations

a) In Chemically or Radiation Mutated Population

Production of chemically or radiation mutated populations is a commontechnique and known to the skilled worker. Methods are described byKoorneef et al. 1982 and the citations therein and by Lightner andCaspar in “Methods in Molecular Biology” Vol 82. These techniquesusually induce pointmutations that can be identified in any known geneusing methods such as TILLING (Colbert et al. 2001).

b) T-DNA or Transposon Mutated Population by Reserve Genetics

Reverse genetic strategies to identify insertion mutants in genes ofinterest have been described for various cases eg. Krysan et al., 1999(Plant Cell 1999, 11, 2283-2290); Sessions et al., 2002 (Plant Cell2002, 14, 2985-2994); Young et al., 2001, (Plant Physiol. 2001, 125,513-518); Koprek et al., 2000 (Plant J. 2000, 24, 253-263); Jeon et al.,2000 (Plant J. 2000, 22, 561-570); Tissier et al., 1999 (Plant Cell1999, 11, 1841-1852); Speulmann et al., 1999 (Plant Cell 1999, 11,1853-1866). Briefly material from all plants of a large T-DNA ortransposon mutagenized plant population is harvested and genomic DNAprepared. Then the genomic DNA is pooled following specificarchitectures as described for example in Krysan et al., 1999 (PlantCell 1999, 11, 2283-2290). Pools of genomics DNAs are then screened byspecific multiplex PCR reactions detecting the combination of theinsertional mutagen (eg T-DNA or Transposon) and the gene of interest.Therefore PCR reactions are run on the DNA pools with specificcombinations of T-DNA or transposon border primers and gene specificprimers. General rules for primer design can again be taken from Krysanet al., 1999 (Plant Cell 1999, 11, 2283-2290) Rescreening of lowerlevels DNA pools lead to the identification of individual plants inwhich the gene of interest is disrupted by the insertional mutagen.

EQUIVALENTS

Those of ordinary skill in the art will recognize, or will be able toascertain using no more than routine experimentation, many equivalentsto the specific embodiments of the invention described herein. Suchequivalents are intended to be encompassed by the following claims.

1. A process for the production of methionine, which comprises thefollowing steps: a) reducing or deleting the expression of at least onenucleic acid molecule in a non-human organism, wherein the at least onenucleic acid molecule is selected from the group consisting of: i) anucleic acid molecule encoding the polypeptide shown in SEQ ID NO: 152,ii) a nucleic acid molecule comprising the nucleotide sequence shown inSEQ ID NO: 151, and iii) a nucleic acid molecule encoding a polypeptidehaving at least 95% identity with the amino acid sequence of SEQ ID NO:152, and b) growing the non-human organism under conditions which permitthe production of methionine in said non-human organism, wherein thenon-human organism is a microorganism or a plant, and wherein reducingor deleting the expression of said at least one nucleic acid moleculeresults in production of methionine.
 2. The process as claimed in claim1, wherein the methionine synthesized by the non-human organism isrecovered.
 3. The process as claimed in claim 1, wherein the reductionor deletion of the expression of the at least one nucleic acid moleculeis caused by a chemical compound.
 4. The process as claimed in claim 1,wherein the non-human organism is a plant.
 5. The process as claimed inclaim 4, wherein the plant is selected from the group consisting ofAnacardiaceae, Asteraceae, Apiaceae, Betulaceae, Boraginaceae,Brassicaceae, Bromeliaceae, Caricaceae, Cannabaceae, Convolvulaceae,Chenopodiaceae, Cucurbitaceae, Elaeagnaceae, Ericaceae, Euphorbiaceae,Fabaceae, Geraniaceae, Gramineae, Juglandaceae, Lauraceae, Leguminosae,Linaceae, perennial grass, fodder crops, vegetables and ornamentals. 6.The process as claimed in claim 1, wherein the microorganism is selectedfrom the group consisting of Actinomycetaceae, Bacillaceae,Brevibacteriaceae, Corynebacteriaceae, Enterobacteriacae, Gordoniaceae,Micrococcaceae, Mycobacteriaceae, Nocardiaceae, Pseudomonaceae,Rhizobiaceae, Streptomycetaceae, Chaetomiaceae, Choanephoraceae,Cryptococcaceae, Cunninghamellaceae, Demetiaceae, Moniliaceae,Mortierellaceae, Mucoraceae, Pythiaceae, Sacharomycetaceae,Saprolegniaceae, Schizosacharomycetaceae, Sodariaceae,Sporobolomycetaceae, Tuberculariaceae, Adelotheciaceae, Dinophyceae,Ditrichaceae and Prasinophyceae.
 7. The process as claimed in claim 1,wherein the at least one nucleic acid molecule encoding the polypeptideshown in SEQ ID NO:
 152. 8. The process as claimed in claim 1, whereinthe at least one nucleic acid molecule comprises the nucleotide sequenceshown in SEQ ID NO:151.